Papers by Pisit Poolprasert
The Synthesis of Molecularly Imprinted Polymers on Microcentrifuge Tube Filters for Solid-Phase Extraction and the HPLC-UV Determination of Andrographolides
Journal of Chromatographic Science
Molecular imprinting-based solid-phase extraction has been in the spotlight to improve recognitio... more Molecular imprinting-based solid-phase extraction has been in the spotlight to improve recognition selectivity and detection sensitivity of andrographolides. The synthesis of molecularly imprinted polymers on micro centrifuge tube filters for the extraction and the determination of andrographolides were investigated. Molecularly imprinted polymers were synthesized using the photo-polymerization method for the preconcentration of andrographolides (AD) template molecule using 2,2-dimethoxy-2-phenylacetophenone as initiators, the mixture of 1-dodecanol and toluene solvent, 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate as functional monomers and cross-linked, respectively. The resultant AD molecularly imprinted polymers (AD-MIPs) were characterized using the Fourier-transform infrared spectrum and scanning electron microscopy. The maximum adsorption of AD-MIPs toward the andrographolides was found to be 85%, and could reach binding equilibrium within 60 min. The sample ...
There is no available report on histological observation of the anterior kidney of the yellow mys... more There is no available report on histological observation of the anterior kidney of the yellow mystus Hemibagrus filamentus (Fang and Chaux, 1949). Thus, the histology of the anterior kidney of specimens from the Tapee river, Chawang district, Nakhon Si Thammarat province, Thailand, was investigated under a light microscope in this study. The results revealed that the anterior kidney of H. filamentus consists of 3 distinct parts; pro, mid and hind anterior kidneys. Based upon different observations of the excretory portion and tissue types, this presented study is considered to be the first such report from Thailand. The pro-anterior kidney comprises of interregnal and exclusively hematopoietic tissues. The mid-anterior kidney is moderately composed of hematopoietic tissue and an increase of the melanomacrophage center (MMC) with renal tubules. The last part of is the anterior kidney, which contains mainly renal tubules with a lesser amount of interstitial hematopoietic, which an increase in the glomerulus.
The histological and histochemical characteristics of the digestive tract of Stoliczkae's barb Pu... more The histological and histochemical characteristics of the digestive tract of Stoliczkae's barb Puntius stoliczkanus (Day, 1871) were examined. This fish had an intermediate gut length with the nonexistence of the stomach. The histology revealed that the intestine consisted of four different layers: mucosa, submucosa, muscularis and serosa. The mucosa comprised the various villi and simple, columnar epithelia which were lying on the lamina propria. Muscularis mucosae and mucosal tubular glands were not present. Eosinophilic granular cells were observed along the intestine, mainly beneath the lamina propria. Histochemical analysis showed the goblet cells and eosinophilic granular cells react positively with periodic acid-Schiff. This research reports on the histological study of the alimentary tract of P. stoliczkanus for the first time.
Context: Microarchitectural observation of the olfactory organ in Devario regina (Fowler, 1934) i... more Context: Microarchitectural observation of the olfactory organ in Devario regina (Fowler, 1934) is still unknown. Objectives: The normal histology and chemical detailed of glycoprotein in D. regina olfactory organ were investigated using histochemical analysis. Materials and Methods: Fishes were collected from the Tapee River, Nakhon Si Thammarat Province, Thailand and were processed by the standard histological technique. Results: Microarchitecture of olfactory organ revealed that it was a paired olfactory sac. Each sac was composed of the olfactory chamber and many lamellae surrounding by olfactory epithelium. This epithelium contained the differential cell types in both sensory (sensory ciliated cells) and non-sensory olfactory epithelium. The special localization of glycoprotein was intensively detected on the mucous cells. Conclusion: This study provided the basic histology of the fish olfactory organ that will support the investigation regarding the physiological and ultrastructural analysis.
Limited research has been reported in the basic information about the structural organizations of... more Limited research has been reported in the basic information about the structural organizations of fish organs in their juvenile state that could be used as histopathological biomarkers. Thus, the histological structures of important organs of the female fish Devario regina (Fowler, 1934) during its juvenile stage were exclusively examined using histological and histochemical approaches. Specimens were collected during the fishing season (July and October 2010) from the Tapee river, Thailand. Using histological analysis, the digestive system was distinctly composed of two parts; the digestive tract and accessory organs (liver and pancreas). Based on their histological structure, the epithelial organization of the oral cavity and pharynx was lined by stratified epithelium whereas the intestine was covered by a simple columnar epithelium and contained several goblet cells. The goblet cells were negatively stained with hematoxylin and eosin (H&E) and Masson's Trichrome (MT). In contrast, they were positively stained with Periodic Acid Schiff reaction (PAS) and aniline blue (AB). The liver tissue in this fish was composed of polyhedral hepatocytes, with their sinusoids being distinctly located between the hepatocytes. The sinusoids were lined by a simple squamous epithelium. The pancreatic parenchyma mainly consisted of pyramidal cells that rested on a basal lamina in the acinar. Moreover, the pancreatic cells had a basophilic cytoplasm, a distinct basal nucleus and contained large eosinophilic zymogen granules. The excretory system especially referred to the kidney, and was composed of renal tubules and hematopoietic tissue. The female reproductive system was the ovary that was surrounded by a tunica albuginea. The ovary contained oocytes at differential stages of development including oogonia and the previtellogenic stage. Finally, the integument of this species consisted mainly of three layers of epidermis, dermis and hypodermis, respectively.
The eye structures and chemical details of the retinal layer in juvenile Queen Danio—Devario regi... more The eye structures and chemical details of the retinal layer in juvenile Queen Danio—Devario regina, an ornamental fish—were histologically investigated under a light microscope. Sample fish were collected from the Tapee River, Nakhon Si Thammarat province, Thailand and their heads were
exclusively prepared using a standard histological technique. The results revealed that the eye of D. regina was composed of three layers—inner, middle and external—based on histological organization and cell types. The inner layer was composed of 10 layers; 1) pigment epithelium, 2) photoreceptor layer, 3) outer liming membrane, 4) outer nuclear layer, 5) outer plexiform layer, 6) inner nuclear layer, 7) inner plexiform layer, 8) ganglion cell layer, 9) optic nerve layer and 10) inner limiting membrane,
respectively. The localization and chemical details showed that a periodic acid-Schiff reaction for the detection of glycoprotein was intensive in the pigment epithelial layer whereas the inner plexifrom layer had only a slight reaction. Reaction of aniline blue was employed for the detection of mucopolysaccharide
which was slightly positive for three layers—the outer limiting membrane, outer plexiform and inner plexiform.
Anopheles dirus females landing on humans inside experimental huts treated with residual applicat... more Anopheles dirus females landing on humans inside experimental huts treated with residual applications of DDT or deltamethrin were observed during the wet season in Pu Teuy Village, Kanchanaburi Province, western Thailand. Two identical experimental huts were constructed in the fashion of typical local rural Thai homes. Pretreatment (baseline) human-landing collections (HLC) in both huts showed an early evening peak of activity between 1900 and 2000 h with no signiÞcant difference in numbers of mosquitoes captured between huts over a period of 30 collection nights. During posttreatment HLC, female mosquitoes continued to show greater landing activity inside huts Þtted with insecticide-treated panels during the Þrst half of the evening compared with the second half. A greater number (proportion) of An. dirus females landed on humans in the hut treated with deltamethrin compared with DDT. Comparing pre-and posttreatment HLC, the DDT-treated hut showed a 79.4% decline in attempted blood feeding, whereas exposure to deltamethrin resulted in a 56.3% human-landing reduction. An odds ratio was performed to demonstrate the relative probability (risk) of mosquitoes entering and attempting to blood feed in the two treated huts compared with untreated control huts. Mosquitoes were 0.47 times less likely to land on humans inside a DDT-treated hut compared with the deltamethrin-treated hut. Although both chemicals exerted strong excitatory responses, DDT appears to have a more pronounced and signiÞcant (P 0.002) effect on behavior than deltamethrin, resulting in greater movement away from the insecticide source and thus potential reduction of blood-feeding activity.
The behavioral and physiological responses of 6-d-old Aedes aegypti (L.) adult females exposed to... more The behavioral and physiological responses of 6-d-old Aedes aegypti (L.) adult females exposed to deltamethrin and DDT were characterized using a free-choice excito-repellency test
system. Excluding varying pretest age and carbohydrate availability as possible confounders, insecticide contact (measuring irritancy) and noncontact (measuring repellency) behavioral assays were conducted on two nonbloodfed groups, either unmated or mated (nulliparous), and two blood-fed
groups, either parous or newly full-engorged mosquitoes. The degree of escape response to deltamethrin and DDT varied according to the physiological conditioning. Escape rates from contact and noncontact chambers with deltamethrin were more conspicuous in nonbloodfed groups compared with mosquitoes previously bloodfed. There were no significant differences in escape responses between unmated and nulliparous test populations. With DDT, a more pronounced escape response was observed in unmated compared with other physiological conditions. More moderate escape
response was seen in nulliparous mosquitoes, and the least was observed in full bloodfed test individuals, regardless of test compound. Ae. aegypti, regardless of pretest conditioning, was completely susceptible to deltamethrin, whereas showing high resistance to DDT. Despite profound differences in resistance, there was no significant difference in avoidance response between chemicals and mosquito conditioning. Moreover, pre- and postbloodmeals were found to influence assay outcome and thus to have relevance on the interpretation of usceptibility and excito-repellency assays.
Biting patterns of natural populations of Anopheles minimus s.l. females entering experimental hu... more Biting patterns of natural populations of Anopheles minimus s.l. females entering experimental huts treated with DDT and deltamethrin were carried out at Pu Teuy Village, Sai Yok District, Kanchanaburi Province, western Thailand. Two experimental huts, control and treatment, were constructed in the fashion of local Thai homes. Pre-spray biting activity of An. minimus females peaked at 19:00-22:00. Post-treatment exposure continued to show greater landing activity during the first half of the evening. An overall greater proportion of An. minimus females entered the hut treated with deltamethrin compared to DDT. The hut fitted with DDT-treated net panels showed a 71.5% decline in attempted blood feeding, whereas exposure to deltamethrin-treated panels resulted in a 42.8% human-landing reduction. DDT exhibited significantly more pronounced (P < 0.05) effects in overall reduction of biting activity than did deltamethrin. Journal of Vector Ecology 33 (2): 285-292. 2008.
A new species of webspinner, Eosembia apterosa Poolprasert and Edgerly, n. sp., from Chiang Mai P... more A new species of webspinner, Eosembia apterosa Poolprasert and Edgerly, n. sp., from Chiang Mai Province in Thailand, is described and illustrated. The new species is distinguished from previously described Eosembia by the occurrence of winglessness in the males. Beyond just winglessness, other morphological characters of the unidentified specimens when compared to E. auripecta Ross and E. aequicercata Ross suggest that they represent a new species. We present the description of the new species, along with a distribution map and key to the currently known species of SE Asian Eosembia.
Four new species of webspinners in the genus Ptilocerembia Friederichs (Ptilocerembiidae) are des... more Four new species of webspinners in the genus Ptilocerembia Friederichs (Ptilocerembiidae) are described including Ptilocerembia thaidina sp. n., P. senathami sp. n., P. catherinae sp. n. and P. rossi sp. n.. from Thailand. Illustrations of heads, genitalia and papilla of adult males, sternite pattern of adult females together with photographs of adult males, females and their galleries for each species are provided. Notes on field observations and egg mass structure are given for P. catherinae sp. n. A distribution map and a dichotomous key to the Thai species in the genus Ptilocerembia are also included.
Isozyme frequencies were compared in seven field collections of Anopheles minimus complex using s... more Isozyme frequencies were compared in seven field collections of Anopheles minimus complex using starch gel electrophoresis. Mosquito collections were sampled from four districts in Kanchanaburi Province where malaria is endemic.
From eight enzyme systems, nine loci and seven polymorphisms were detected, indicating limited genetic differentiation among the seven collections (FST = 0.061). The highest percent polymorphic loci were observed in Bong Ti Noi (BTN) Village (55.6%), whereas the least percent polymorphism was seen in Tha Kradan (TK) Village (22.2%). Comparing villages
Pra Jedee (PJ) with Pu Teuy C (PTC) and Huai Khayeng (HK) with Pra Jedee (PJ), gene flow among collections varied from 3.72 to 62.25 reproductive migrants per generation. Among the seven collections, no correlation was seen between genetic and geographical distances (P > 0.05). Anopheles minimus (former species A) and Anopheles harrisoni (former species C)
from Pu Teuy fit most closely in the same cluster, possibly indicating relatively recent divergence between taxa. The genetic and epidemiological ramifications of these findings are discussed.
Three embiid species of the genus Aposthonia Krauss (Oligotomidae) are recognized from Thailand (... more Three embiid species of the genus Aposthonia Krauss (Oligotomidae) are recognized from Thailand (Aposthonia borneen-sis, A. ceylonica, A. problita sp. n.) and the new species is described. The distribution of the three species in Thailand is shown and keys to the males of 25 species of this genus and to females found in Thailand are provided.
Two new species of webspinners (Embioptera) in the genus Eosembia Ross from Thailand, E. lamunae ... more Two new species of webspinners (Embioptera) in the genus Eosembia Ross from Thailand, E. lamunae sp. n. and E. par-adorni sp. n., are described and illustrated. These species bring the total number of species known from eastern, southeastern and southern Asia to 15 species. An identification key to the currently recognized species in the genus Eosembia, based on adult males, is included.
Dachtylembia gen. nov. (Embioptera: Teratembiidae), is described and illustrated based on specime... more Dachtylembia gen. nov. (Embioptera: Teratembiidae), is described and illustrated based on specimens of a new species (D. siamensis) collected from Thailand. The geographical distribution of this species in Thailand is mapped.
Field sampling was performed at 17 locations within 14 provinces in Thailand by hand collection ... more Field sampling was performed at 17 locations within 14 provinces in Thailand by hand collection from January 2008 to July 2011 and resulted in the collection of three distinct species of Oligotoma—namely, O. humbertiana (Saussure, 1896), O. nigra (Hagen, 1885) and O. saundersii (Westwood, 1837)—that are new records for Thailand. Details of the important morphological characteristics of the female were added and illustrated. Identification keys to the three species, for both male and female adults, were compiled and the geographic distributions of the three species in this genus in Thailand
were also mapped.
A determination was conducted in eastern Thailand of the family composition of dung-utilizing ins... more A determination was conducted in eastern Thailand of the family composition of dung-utilizing insects in relation to various elephant dung pad stages: A (dung intact with odor and moisture), B (dung intact, no odor, no moisture), C 1 (less than 50% degraded), C 2 (50% or more degraded) and stage D (flat mass). A total of 4,857 individuals representing 30 families of insects utilized the sampled elephant dung; these included beetles from three prominent families: Ptiliidae, Staphylinidae and Scarabaeidae. Scarab beetles could be found throughout all stages of dung disintegration. The Shannon-Wiener index (H') for all insects in all elephant dung piles was considered relatively high. In addition, H' obtained by calculating the pooled data set of this study was 2.09, with high evenness (E = 0.615), and dominance by the Simpson index was high for all dung-utilizing insects (D = 0.723). Stage B showed the highest family diversity index (H' = 2.274), whereas stage C 1 had the lowest levels of diversity (H' = 1.401). Significant differences in the family diversity indices of five Asian elephant dung stages were detected (P < 0.05) in all comparisons. The results show that such biodiversity parameters as the richness, diversity and composition of these insect communities differ during the various stages of elephant dung decay. The relevance of these findings to the interpretation of what constitutes a dung stage preference is discussed.
There is no available report on histological observation of the anterior kidney of the yellow mys... more There is no available report on histological observation of the anterior kidney of the yellow mystus Hemibagrus filamentus (Fang and Chaux, 1949). Thus, the histology of the anterior kidney of specimens from the Tapee river, Chawang district, Nakhon Si Thammarat province, Thailand, was investigated under a light microscope in this study. The results revealed that the anterior kidney of H. filamentus consists of 3 distinct parts; pro, mid and hind anterior kidneys. Based upon different observations of the excretory portion and tissue types, this presented study is considered to be the first such report from Thailand. The pro-anterior kidney comprises of interregnal and exclusively hematopoietic tissues. The mid-anterior kidney is moderately composed of hematopoietic tissue and an increase of the melanomacrophage center (MMC) with renal tubules. The last part of is the anterior kidney, which contains mainly renal tubules with a lesser amount of interstitial hematopoietic, which an increase in the glomerulus.
The histological and histochemical characteristics of the digestive tract of Stoliczkae's barb Pu... more The histological and histochemical characteristics of the digestive tract of Stoliczkae's barb Puntius stoliczkanus (Day, 1871) were examined. This fish had an intermediate gut length with the nonexistence of the stomach. The histology revealed that the intestine consisted of four different layers: mucosa, submucosa, muscularis and serosa. The mucosa comprised the various villi and simple, columnar epithelia which were lying on the lamina propria. Muscularis mucosae and mucosal tubular glands were not present. Eosinophilic granular cells were observed along the intestine, mainly beneath the lamina propria. Histochemical analysis showed the goblet cells and eosinophilic granular cells react positively with periodic acid-Schiff. This research reports on the histological study of the alimentary tract of P. stoliczkanus for the first time.
The histology of the organization of the eye in the Stoliczkae's barb Puntius stoliczkanus was in... more The histology of the organization of the eye in the Stoliczkae's barb Puntius stoliczkanus was investigated using a light microscope. It was found that the eye in P. stoliczkanus comprised three layers—external, middle uveal and inner. The external layer consisted of the cornea and sclera. The cornea showed three layers: 1) corneal epithelium, 2) corneal stroma and 3) corneal endothelium, while the sclera exhibited a thick connective fi ber and an external sheath of hyaline cartilage supporting the sclera. The middle uveal layer showed only the lens and choroid. The lens protruded into the aqueous chamber. The choroid was subdivided into two distinct layers: 1) a connective layer adjacent to the sclera and 2) the lamina vasculosa and choriocapillaris. The inner layer or retinal tunica was composed of 10 distinct layers: 1) pigment epithelium, 2) photoreceptor layer, 3) outer liming membrane, 4) outer nuclear layer, 5) outer plexiform layer, 6) inner nuclear layer, 7) inner plexiform layer, 8) ganglion cell layer, 9) optic nerve layer and 10) inner limiting membrane, respectively, from the outside inward. These results provided data for other investigations in aquaculture, reproductive biology and histopathology.
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Papers by Pisit Poolprasert
exclusively prepared using a standard histological technique. The results revealed that the eye of D. regina was composed of three layers—inner, middle and external—based on histological organization and cell types. The inner layer was composed of 10 layers; 1) pigment epithelium, 2) photoreceptor layer, 3) outer liming membrane, 4) outer nuclear layer, 5) outer plexiform layer, 6) inner nuclear layer, 7) inner plexiform layer, 8) ganglion cell layer, 9) optic nerve layer and 10) inner limiting membrane,
respectively. The localization and chemical details showed that a periodic acid-Schiff reaction for the detection of glycoprotein was intensive in the pigment epithelial layer whereas the inner plexifrom layer had only a slight reaction. Reaction of aniline blue was employed for the detection of mucopolysaccharide
which was slightly positive for three layers—the outer limiting membrane, outer plexiform and inner plexiform.
system. Excluding varying pretest age and carbohydrate availability as possible confounders, insecticide contact (measuring irritancy) and noncontact (measuring repellency) behavioral assays were conducted on two nonbloodfed groups, either unmated or mated (nulliparous), and two blood-fed
groups, either parous or newly full-engorged mosquitoes. The degree of escape response to deltamethrin and DDT varied according to the physiological conditioning. Escape rates from contact and noncontact chambers with deltamethrin were more conspicuous in nonbloodfed groups compared with mosquitoes previously bloodfed. There were no significant differences in escape responses between unmated and nulliparous test populations. With DDT, a more pronounced escape response was observed in unmated compared with other physiological conditions. More moderate escape
response was seen in nulliparous mosquitoes, and the least was observed in full bloodfed test individuals, regardless of test compound. Ae. aegypti, regardless of pretest conditioning, was completely susceptible to deltamethrin, whereas showing high resistance to DDT. Despite profound differences in resistance, there was no significant difference in avoidance response between chemicals and mosquito conditioning. Moreover, pre- and postbloodmeals were found to influence assay outcome and thus to have relevance on the interpretation of usceptibility and excito-repellency assays.
From eight enzyme systems, nine loci and seven polymorphisms were detected, indicating limited genetic differentiation among the seven collections (FST = 0.061). The highest percent polymorphic loci were observed in Bong Ti Noi (BTN) Village (55.6%), whereas the least percent polymorphism was seen in Tha Kradan (TK) Village (22.2%). Comparing villages
Pra Jedee (PJ) with Pu Teuy C (PTC) and Huai Khayeng (HK) with Pra Jedee (PJ), gene flow among collections varied from 3.72 to 62.25 reproductive migrants per generation. Among the seven collections, no correlation was seen between genetic and geographical distances (P > 0.05). Anopheles minimus (former species A) and Anopheles harrisoni (former species C)
from Pu Teuy fit most closely in the same cluster, possibly indicating relatively recent divergence between taxa. The genetic and epidemiological ramifications of these findings are discussed.
were also mapped.