Papers by Thiruvengadam Thirumurugan
Identification And Functional Diversity Of HAP Gene Families In Rice
Crop Science, 2004
ture of female line seed in the hybrid seed results in yield reduction of 100 kg per hectare (Mao... more ture of female line seed in the hybrid seed results in yield reduction of 100 kg per hectare (Mao et al., 1996). Ensuring the genetic purity of parental lines and hybrids is a prereq-The Indian seed act prescribes that, for hybrid rice, uisite to realize their full potential. The cytoplasmic male sterile (CMS) the purity should be 98% (Verma, 1996), while in the lines that are utilized for developing the popular "three-line" hybrids, often get contaminated with their isonuclear maintainer lines during People's Republic of China it is mandated that the purity CMS line multiplication. Use of such CMS lines in hybrid seed produc-of hybrid rice should be at least 96% (Yan, 2000). To tion results in the production of genetically impure hybrid seed. We ensure the required levels of purity in hybrid seed, the report the identification of a DNA sequence that is homologous to parental lines that are utilized in hybrid seed production rice mitochondrial DNA but unique to the Wild Abortive (WA) should have a very high (about 99%) level of purity. cytoplasmic male sterile lines of rice (Oryza sativa L.). In a polymerase One of the common admixtures that is observed durchain reaction (PCR) using total genomic DNA as a template, oligoing hybrid seed production is that of maintainer lines nucleotide primers based on this unique DNA sequence could amplify with those of the CMS lines. Because these are isoa fragment from CMS lines of rice and their hybrids but not from nuclear, it is not possible to distinguish between them their cognate maintainer lines. In tests on mixed samples of plants until they flower. The availability of appropriate DNA containing both CMS and Maintainer lines, this PCR assay was used markers would greatly facilitate the screening of seed to correctly predict the genotypes of these plants indicating that it lots for contamination of the CMS line with male fertile can be used to detect the mixture of maintainer lines in the seed lines. RFLPs that distinguish WA-CMS lines from their stocks of the CMS line.
Assessment of purity of rice hybrids using microsatellite and STS markers
Crop Science, 2002
... J. Yashitola a ,; T. Thirumurugan b ,; RM Sundaram b ,; MK Naseerullah c ,; MS Ramesha b ,; N... more ... J. Yashitola a ,; T. Thirumurugan b ,; RM Sundaram b ,; MK Naseerullah c ,; MS Ramesha b ,; NP Sarma b and; Ramesh V. Sonti * a. a Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad-500 007, India b Directorate of Rice Research, Rajendranagar, Hyderabad ...
Studies on OsHAP2,OsHAP3 and OsHAP5 gene family members in rice with biological characterization of OsHAP3E
Studies on OsHAP2,OsHAP3 and OsHAP5 gene family members in rice with biological characterization of OsHAP3E
Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation
Theoretical and Applied Genetics, 2004
Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia... more Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. The deployment of molecular markers linked to gall midge resistance genes in breeding programmes can accelerate the development of resistant cultivars. In the present study, we have tagged and mapped a dominant gall midge resistance gene, Gm1, from the Oryza sativa cv. W1263 on chromosome 9, using SSR markers. A progeny-tested F2 mapping population derived from the cross W1263/TN1 was used for analysis. To map the gene locus, initially a subset of the F2 mapping population consisting of 20 homozygous resistant and susceptible lines each was screened with 63 parental polymorphic SSR markers. The SSR markers RM316, RM444 and RM219, located on chromosome 9, are linked to Gm1 at genetic distances of 8.0, 4.9 and 5.9 cM, respectively, and flank the gene locus. Further, gene/marker order was also determined. The utility of the co-segregating SSR markers was tested in a backcross population derived from the cross Swarna/W1263//Swarna, and allelic profiles of these markers were analysed in a set of donor rice genotypes possessing Gm1 and in a few gall midge-susceptible, elite rice varieties.
Plant Science, 2011
We generated transgenic rice plants overexpressing OsHAP3E which encodes a subunit of a CCAAT-mot... more We generated transgenic rice plants overexpressing OsHAP3E which encodes a subunit of a CCAAT-motif binding HAP complex. The OsHAP3E-overexpressing plants showed various abnormal morphologies both in their vegetative and reproductive phases. The OsHAP3E-overexpressing plants were dwarf with erected leaves and similar to brassinosteroid mutants in the vegetative phase. In the reproductive phase, dense panicle was developed, and occasionally successive generation of lateral rachises and formation of double flowers were observed. These phenotypes indicate association of OsHAP3E with determination of floral meristem identity. On the other hand, repression of OsHAP3E by RNAi or by overexpressing chimeric repressor fusion constructs brought about lethality to transformed cells, and almost no transformant was obtained. This suggests that the OsHAP3E function is essential for rice cells. Altogether, our lossof-function and gain-of-function analyses suggest that OsHAP3E plays important pleiotropic roles in vegetative and reproductive development or basic cellular processes in rice.
Identification, characterization and interaction of HAP family genes in rice
Molecular Genetics and Genomics, 2008
A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (... more A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.
Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation
Theoretical and Applied Genetics, 2004
Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia... more Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. The deployment of molecular markers linked to gall midge resistance genes in breeding programmes can accelerate the development of resistant cultivars. In the present study, we have tagged and mapped a dominant gall midge resistance gene, Gm1, from the Oryza sativa cv. W1263 on chromosome 9, using SSR markers. A progeny-tested F2 mapping population derived from the cross W1263/TN1 was used for analysis. To map the gene locus, initially a subset of the F2 mapping population consisting of 20 homozygous resistant and susceptible lines each was screened with 63 parental polymorphic SSR markers. The SSR markers RM316, RM444 and RM219, located on chromosome 9, are linked to Gm1 at genetic distances of 8.0, 4.9 and 5.9 cM, respectively, and flank the gene locus. Further, gene/marker order was also determined. The utility of the co-segregating SSR markers was tested in a backcross population derived from the cross Swarna/W1263//Swarna, and allelic profiles of these markers were analysed in a set of donor rice genotypes possessing Gm1 and in a few gall midge-susceptible, elite rice varieties.
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Papers by Thiruvengadam Thirumurugan