Papers by jerrine joseph
Acinetobacter baumannii is opportunistic Gram-negative bacterial pathogen which causes hospital-a... more Acinetobacter baumannii is opportunistic Gram-negative bacterial pathogen which causes hospital-acquired infections. Existence of numerous antibiotic-resistant strains of A. baumanii emphasizing the need for the development of new antimicrobials to combat them. Alanineracemase (Alr) is a pyridoxal 5'-phosphate dependent enzyme present in A. baumanii is responsible for racemization between enantiomers of alanine. As D-alanine is an essential component of the bacterial cell wall, its inhibition is lethal to prokaryotes, making it an excellent antibiotic drug target. The structure of Alr Aba provides the template required for future structure-based drug-design. In this study, insilico docking studies of the sideromycins albomycin and salmycin against alanineracemase was performed. The binding energy and docked poses evaluation enabled us to conclude that the sideromycins could serve as potential inhibitor of alanine racemase which would be useful in the fight against A. baumanii .
Effect of seaweed on seed germination and biochemical constituents of Capsicum annuum
Biocatalysis and Agricultural Biotechnology
Antiseptic Efficacy of Combination of Certain Essential Oils Against Multi Drug Resistant Pathogens
Bioscience Biotechnology Research Communications
Microbial degradation, spectral analysis and toxicological assessment of malachite green by Streptomyces chrestomyceticus S20
Bioprocess and Biosystems Engineering
Bioresources and Bioprocessing
Background E-waste management is extremely difficult to exercise owing to its complexity and haza... more Background E-waste management is extremely difficult to exercise owing to its complexity and hazardous nature. Printed circuit boards (PCBs) are the core components of electrical and electronic equipment, which generally consist of polymers, ceramics, and heavy metals. Results The present study has been attempted for removal of heavy metals from printed circuit board by metal-resistant actinobacterium Streptomyces albidoflavus TN10 isolated from the termite nest. This bacterium was found to recover different heavy metals (Al 66%, Ca 74%, Cu 68%, Cd 65%, Fe 42%, Ni 81%, Zn 82%, Ag 56%, Pb 46%) within 72 h under laboratory conditions. The metal content of PCB after bioleaching was analyzed by ICP-MS. The crude PCB and bioleaching residue were characterized by FT-IR, XRD, SEM for the determination of structural and functional group changes for confirmation of bioleaching. Conclusion The findings of the present study concluded that Streptomyces albidoflavus TN10 is a promising candidate...
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: Phytochemical is naturally present in the seaweeds which biologically play a significa... more Objective: Phytochemical is naturally present in the seaweeds which biologically play a significant role. The intention of this study was designed to screen the phytochemical constituents and antimicrobial potential of selected seaweed collected from Rameshwaram and Tuticorin Southern coast of India.Methods: The present study investigated the presence of phytochemical constituents and also total phenol, total carbohydrate and total protein quantity of the brown seaweed. Dictyopteris delicatula, Padina gymnospora, Acanthophora spicifera, Portieria hornemannii and Ulva faciata were extracted with solvents having different polarities like methanol, ethanol, chloroform and water and screened for the phytochemical constituents, total phenol, total carbohydrate, total protein and DPPH with standard procedure. The antibacterial activities of the seaweeds were examined by agar well diffusion method.Results: Among the five seaweeds, U. faciata showed the maximum number of active constituents...
Metagenomic analysis holds immense potential for identifying rare and uncharacterized microorgani... more Metagenomic analysis holds immense potential for identifying rare and uncharacterized microorganisms from many ecological habitats. Actinobacteria have been proved to be an excellent source of novel antibiotics for several decades. The present study was designed to delineate and understand the bacterial diversity with special focus on Actinobacteria from pond sediment collected from Sanjeeviraya Hanuman Temple, Ayyangarkulam, Kanchipuram, Tamil Nadu, India. The sediment had an average temperature (25.32%), pH (7.13), salinity (0.960 mmhos/cm) and high organic content (10.7%) posing minimal stress on growth condition of the microbial community. Subsequent molecular manipulations, sequencing and bioinformatics analysis of V3 and V4 region of 16S rRNA metagenomics analysis confirmed the presence of 40 phyla, 100 classes, 223 orders, 319 families and 308 genera in the sediment sample dominated by Acidobacteria (18.14%), Proteobacteria (15.13%), Chloroflexi (12.34), Actinobacteria (10.84...
Bioinformation
To study the involvement of compounds stigmasterol and oleic acid isolated from marine sponge Aur... more To study the involvement of compounds stigmasterol and oleic acid isolated from marine sponge Aurora globostellata and docking against the Human Epidermal Growth Factor Receptor-2 in breast cancer. The comparative molecular docking was performed with the natural compounds from marine sponge and the synthetic drugs used in breast cancer treatment against the target HER2. The molecular docking analysis was done using GLIDE in Schrodinger software package. The ADME properties were calculated using the Qikprop. The observation of the common binding site for all the ligands confirms the binding pocket; where the isolated compound Stigmasterol agrees well with the binding residues and thus can be optimized further to arrive at a molecule that has a high binding affinity and low binding constant. The results of the docking studies carried out on HER2 provide an insight for the compound stigmasterol to have drug like properties than oleic acid. These results are supportive to confirm the marine sponges as a better lead for cancer therapeutics.
Bioinformation
The development of shrimp aquaculture has been severely affected by viral diseases resulting in a... more The development of shrimp aquaculture has been severely affected by viral diseases resulting in a huge economic burden to the industry. White spot disease (WSD) has caused severe mortality in farmed shrimp in many countries. Globally aquaculture industries face huge economic losses due to rapid spread of White Spot Syndrome Virus (WSSV) disease that can cause 100% mortality in a short period of 3-10 days. In the present study in order to prevent the spread of WSSV disease in shrimps, the receptor, PmRab7 has been chosen as the drug target. Due to the absence of a precise 3D structure of the target, homology-modeling approach was employed to obtain the structure that was validated later. This structure was then used as a template to screen selective phytomolecules as potential antiviral agents and their docking results with the target are analyzed based on their energy scores. Identification of the drug-like molecule obtained from the docking analysis would be used to optimize to a candidate drug. This is expected to play a role of the inhibitor that blocks the binding of the viral protein to the receptor, duly preventing the WSSV disease.
Asian Pacific Journal of Tropical Biomedicine, 2012
In silico sequence and structure analysis for mycobacteriophages
Asian Pacific Journal of Tropical Biomedicine, 2012
... Nigel 94 81 13 8 5 0 Ramsey 108 82 26 10 16 0 Solon 86 65 21 10 11 1 adjutor 86 74 12 2 10 1 ... more ... Nigel 94 81 13 8 5 0 Ramsey 108 82 26 10 16 0 Solon 86 65 21 10 11 1 adjutor 86 74 12 2 10 1 angel 61 49 12 6 6 0 ... J Mol Biol 2001; 307(4): 1113-1143. [4] Bateman A, Birney E, Cerruti L, Durbin R, Etwiller L, Eddy SR, et al. The Pfam protein families database. ...
Bioinformation, 2011
Mycobacteriophage genome database (MGDB) is an exclusive repository of the 64 completely sequence... more Mycobacteriophage genome database (MGDB) is an exclusive repository of the 64 completely sequenced mycobacteriophages with annotated information. It is a comprehensive compilation of the various gene parameters captured from several databases pooled together to empower mycobacteriophage researchers. The MGDB (Version No.1.0) comprises of 6086 genes from 64 mycobacteriophages classified into 72 families based on ACLAME database. Manual curation was aided by information available from public databases which was enriched further by analysis. Its web interface allows browsing as well as querying the classification. The main objective is to collect and organize the complexity inherent to mycobacteriophage protein classification in a rational way. The other objective is to browse the existing and new genomes and describe their functional annotation.
articles by jerrine joseph
Journal of Biomolecular Structure and Dynamics, 2020
SARS-CoV-2 a virus which caused the global pandemic the Coronavirus Disease- 2019 (COVID-2019) ha... more SARS-CoV-2 a virus which caused the global pandemic the Coronavirus Disease- 2019 (COVID-2019) has infected about 1,203,959 patients and brought forth death rate about 64,788 among 206 countries as mentioned by WHO in the month of April 2020. The clinical trials are underway for Remdesivir, an investigational anti-viral drug from Gilead Sciences. Antimalarial drugs such as Chloroquine and Hydroxychloroquine derivatives are being used in emergency cases; however, they are not suitable for patients with conditions like diabetes, hypertension and cardiac issues. The lack of availability of approved treatment for this disease calls forth the scientific community to find novel compounds with the ability to treat it. This paper evaluates the compound Andrographolide from Andrographis paniculata as a potential inhibitor of the main protease of SARS-COV-2 (Mpro) through in silico studies such as molecular docking, target analysis, toxicity prediction and ADME prediction. Andrographolide was docked successfully in the binding site of SARS-CoV-2 Mpro. Computational approaches also predicts that this molecule to have good solubility, pharmacodynamics property and target accuracy. This molecule also obeys Lipinski’s rule, which makes it a promising compound to pursue further in biochemical and cell based assays to explore its potential for use against COVID-19.
Uploads
Papers by jerrine joseph
articles by jerrine joseph