PhD Medical Biochemistry Thesis work by Vishnu Kumar
Abstract The present study was undertaken to evaluate
antidiabetic and antioxidant activities of ... more Abstract The present study was undertaken to evaluate
antidiabetic and antioxidant activities of Cassia tora (C.
tora) seeds extract against streptozotocin induced diabetes
in experimental rats to scientifically validate its use against
diabetes. Ethanolic extract of C. tora seeds extract and
standard drug (glibenclamide) prepared in aqueous gum
acacia (2 %, w/v) suspension and fed orally to streptozotocin
induced male adult diabetic rats of Charles Foster
strain for 15 days. Biochemical parameters in normal,
diabetic control, standard (600 lg/kg bw p.o.) and treated
(500 mg/kg bw p.o.) animal groups were quantified and
compared. Treatment of streptozotocin induced diabetic
rats with ethanolic seeds extract caused significant
(p\0.001) reduction in blood glucose (270–220 mg/dl),
total cholesterol (140–104 mg/dl), triglyceride
(149–99 mg/dl), phospholipids (100–74 mg/dl), free fatty
acid (2.39–2.00 lmol/l), lipid peroxide (9–5.63 nmol
MDA/dl) and significantly increased post heparin lipolytic
activity (11–14 nmol FFA released/h/l plasma)
(p\0.001). Furthermore, the seeds extract (100–400 lg)
when tested for its antioxidant activity in vitro, showed
significant (p\0.001) inhibition in the generation of super
oxide anions in enzymic system a (46–37, 33, 23, 21 nmol
uric acid formed/min), in enzymic system b (113–91, 77,
60, 51 nmol formazon formed/min), non-enzymic system
(324–230, 211, 161, 141 nmol uric acid formed/min) and
hydroxyl radicals in enzymic system (544–501, 411, 319,
291 nmol 2,3-dihydroxybenzoate formed/h) and non-enzymic
system (28–21, 17, 14, 12). The results of the present
study demonstrated antidiabetic, antidyslipidemic and
antioxidant activities of C. tora seeds which could help in
prevention of diabeticdyslipidemia and related
complications.
Keywords Cassia tora seeds extract Anti-dyslipidemic
agent Post heparin lipolytic activity Streptozotocin
induced diabetes Plant antioxidant
The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxida... more The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxidant activities of Tinospora cordifolia (Menispermaceae) in type 2 diabetic patients. In this study clinical trial was conducted on type 2 diabetic patients attending the diabetes OPD, at Kaya Chikitsha Vibhag, Rajkiya Ayurvedic Chikitsha Mahavidyalaya, Touriya Ganj, Lucknow, following all ethical guide lines. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein were noted in type 2 diabetic patients compared to healthy controls. The results after prescription of Tinospora cordifoia (powered stem; 50 mg/kg body weight, p. o.) for 15 days in type 2 diabetic patients resulted significant decrease in the level of fasting blood sugar, total cholesterol, β lipoproteins and triglycerides. The decrease in lipids and apoprotein levels of β lipoproteins was accompanied with stimulation of plasma lecithin cholesterol acyltransferase (LCAT). Lipid and apoprotein level of α lipoproteins were also recovered partially. Results of this study may provide scope for further research and leading to development of a potent anti diabetic, antidyslipoproteinemic anti oxidant drug from Tinospora cordifolia (T. cordifolia) stem.
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PhD Medical Biochemistry Thesis work by Vishnu Kumar
antidiabetic and antioxidant activities of Cassia tora (C.
tora) seeds extract against streptozotocin induced diabetes
in experimental rats to scientifically validate its use against
diabetes. Ethanolic extract of C. tora seeds extract and
standard drug (glibenclamide) prepared in aqueous gum
acacia (2 %, w/v) suspension and fed orally to streptozotocin
induced male adult diabetic rats of Charles Foster
strain for 15 days. Biochemical parameters in normal,
diabetic control, standard (600 lg/kg bw p.o.) and treated
(500 mg/kg bw p.o.) animal groups were quantified and
compared. Treatment of streptozotocin induced diabetic
rats with ethanolic seeds extract caused significant
(p\0.001) reduction in blood glucose (270–220 mg/dl),
total cholesterol (140–104 mg/dl), triglyceride
(149–99 mg/dl), phospholipids (100–74 mg/dl), free fatty
acid (2.39–2.00 lmol/l), lipid peroxide (9–5.63 nmol
MDA/dl) and significantly increased post heparin lipolytic
activity (11–14 nmol FFA released/h/l plasma)
(p\0.001). Furthermore, the seeds extract (100–400 lg)
when tested for its antioxidant activity in vitro, showed
significant (p\0.001) inhibition in the generation of super
oxide anions in enzymic system a (46–37, 33, 23, 21 nmol
uric acid formed/min), in enzymic system b (113–91, 77,
60, 51 nmol formazon formed/min), non-enzymic system
(324–230, 211, 161, 141 nmol uric acid formed/min) and
hydroxyl radicals in enzymic system (544–501, 411, 319,
291 nmol 2,3-dihydroxybenzoate formed/h) and non-enzymic
system (28–21, 17, 14, 12). The results of the present
study demonstrated antidiabetic, antidyslipidemic and
antioxidant activities of C. tora seeds which could help in
prevention of diabeticdyslipidemia and related
complications.
Keywords Cassia tora seeds extract Anti-dyslipidemic
agent Post heparin lipolytic activity Streptozotocin
induced diabetes Plant antioxidant