Vishnu Kumar
Professor and HOD Biochemistry
Madhav Prasad Tripathi Government Medical College Sidharth Nagar Uttar Pradesh
Supervisors: PROFESSOR AND HOD BIOCHEMISTRY and Professor and HOD Biochemistry
Madhav Prasad Tripathi Government Medical College Sidharth Nagar Uttar Pradesh
Supervisors: PROFESSOR AND HOD BIOCHEMISTRY and Professor and HOD Biochemistry
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Papers by Vishnu Kumar
antidiabetic and antioxidant activities of Cassia tora (C.
tora) seeds extract against streptozotocin induced diabetes
in experimental rats to scientifically validate its use against
diabetes. Ethanolic extract of C. tora seeds extract and
standard drug (glibenclamide) prepared in aqueous gum
acacia (2 %, w/v) suspension and fed orally to streptozotocin
induced male adult diabetic rats of Charles Foster
strain for 15 days. Biochemical parameters in normal,
diabetic control, standard (600 lg/kg bw p.o.) and treated
(500 mg/kg bw p.o.) animal groups were quantified and
compared. Treatment of streptozotocin induced diabetic
rats with ethanolic seeds extract caused significant
(p\0.001) reduction in blood glucose (270–220 mg/dl),
total cholesterol (140–104 mg/dl), triglyceride
(149–99 mg/dl), phospholipids (100–74 mg/dl), free fatty
acid (2.39–2.00 lmol/l), lipid peroxide (9–5.63 nmol
MDA/dl) and significantly increased post heparin lipolytic
activity (11–14 nmol FFA released/h/l plasma)
(p\0.001). Furthermore, the seeds extract (100–400 lg)
when tested for its antioxidant activity in vitro, showed
significant (p\0.001) inhibition in the generation of super
oxide anions in enzymic system a (46–37, 33, 23, 21 nmol
uric acid formed/min), in enzymic system b (113–91, 77,
60, 51 nmol formazon formed/min), non-enzymic system
(324–230, 211, 161, 141 nmol uric acid formed/min) and
hydroxyl radicals in enzymic system (544–501, 411, 319,
291 nmol 2,3-dihydroxybenzoate formed/h) and non-enzymic
system (28–21, 17, 14, 12). The results of the present
study demonstrated antidiabetic, antidyslipidemic and
antioxidant activities of C. tora seeds which could help in
prevention of diabeticdyslipidemia and related
complications.
Keywords Cassia tora seeds extract Anti-dyslipidemic
agent Post heparin lipolytic activity Streptozotocin
induced diabetes Plant antioxidant
Diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia with glycosuria and it is well documented that increased level of blood glucose is a marker of disorder of carbohydrate metabolism and is associated with the initiation of diabetic dyslipoproteinemia and other complications. Prolonged free radical mediated lipotoxicity and abnormal glucose tolerance are involved in the pathogenesis of diabetes mellitus. Diabetic dyslipoproteinemia is characterized by the increased level of cholesterol, reduced HDL and high triglyceride (TG) levels.The present study was carried out to explore the status of oxidative stress, antioxidant enzymes, lipids and lipoprotein profile in type-2 diabetic patients. This study was conducted on type 2 diabetic patients attending the diabetes OPD, Era’s Lucknow Medical & Hospital, Sarfaraz Ganj, Hardoi Road, Lucknow. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid peroxide, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein, antioxidant enzymes and reduced glutathione were noted in type 2 diabetic patients compared to healthy controls. Type 2 diabetic patients are consistently associated with disorder of metabolism and disturb redox status.
Key words: Lipid profile-Lipoprotein profile-Oxidative stress-Antioxidants-LCAT.
Hyperglycemia, oxidative stress and dyslipoproteinemia are well known factors for Coronary artery disease (CAD) which is the major cause of mortality and morbidity worldwide. The incident of CAD with type 2 diabetes mellitus (T2DM) is rising and they are predicted to be the biggest cause of death by 2020 in India. Therefore, the aim of the present study was to assess the association of hyperglycemia, oxidative stress, antioxidant enzymes, lipid profile and lipoprotein sub fractions in CAD with T2DM patients as well as to compare the results with age-sex matched healthy human volunteers. Three hundred participants were enrolled for the present study, with their ages ranging from 45 to 55 years from Era’s Lucknow Medical College & Hospital, Lucknow. Out of which one hundred fifty were clinically new diagnosed case of CAD with T2DM like hyperglycemia, angina pectoris and Myocardial infarction (MI), remaining one hundred fifty were healthy controls. All biochemical assays were carried out by the standard kit methods. These participants were investigated for serum lipid profile, Lipoprotein sub fractions along with blood levels of lipid peroxide, reduced glutathione and antioxidant enzymes. A marked impairment in plasma levels of lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein, reduced glutathione as well as level of antioxidant enzymes were noted in CAD with T2DM patients with respected to healthy controls.
cordifolia (T. cordifolia): family Menispermaceae, Hindi
name: Giloya; Gurch or Tinospora in english, extract have
been studied in alloxan induced diabetic rats. In this model,
oral administration of stem extract (500 mg/kg bw. p.o.)
for 30 days resulted significant decreased in the levels of
blood glucose, plasma lipids and reactivated post heparin
lipoprotein lipase activity in alloxan induced diabetic rats.
Furthermore, the stem extract (50–500 lg) when tested for
its antioxidant activity, shown to inhibit the generation of
super oxide anions and hydroxyl radicals, in both enzymic
and non-enzymic systems in vitro. The results of the present
study demonstrated antidyslipidemic and antioxidant
activities of T. cordifolia which could help in prevention of
diabetic-dyslipidemia and related complications.
Keywords Tinospora cordifolia Anti-dyslipidemic
agent Natural antioxidants Post heparin lipolytic
activity Alloxan induced-diabetes Hypoglycemic agent
HOSPITAL LABORATORY SERVICES,
ERA’S LUCKNOW MEDICAL COLLEGE & HOSPITAL (ELMC&H),
SARFARAZ GANJ, HARDOI ROAD, LUCKNOW – 226003
DOCUMENT NO. : NABL 160
ISSUE DATE : dd/mm /yy
COPY NO. :
ISSUED BY : Dr…………………………
HOLDER’S NAME :
QUALITY MANAGER : DR VISHNU KUMAR
ERA’S LUCKNOW MEDICAL COLLEGE & HOSDPITAL, SARFARAZ GANJ, HARDOI ROAD, LUCKNOW - 226003
QUALITY MANUAL
(Written by Dr Vishnu Kumar)
The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract have been studied in alloxan-induced diabetic rats. In this model oral administration of seed extract (500 mg/kg bw. P. o.) for 15 days in diabetic-dyslipidemic rats exerted significant lipid lowering effect as assessed by the reversal of serum levels of total cholesterol, phospholipids, triglyceride, free fatty acids, protein and lipid peroxide. The decrease of lipids and apoprotein levels of Very Low Density Lipoprotein and Low Density Lipoprotein were followed by stimulation of plasma post-heparin lipolytic activity as well as lecithin cholesterol acyltransferase, hepatic superoxide dismutase, catalase, triglyceride lipase and lipoprotein lipase activities with increase in reduced glutathione. Lipid and apoprotein level of High Density Lipoprotein were also recovered partially on treatment with seeds extract. The results of the present study demonstrated antidyslipidemic and antioxidant activities in seed extract of C. tora which could be used in prevention of diabetic dyslipidemia and related complications. The hypolipidemic activity of Cassia tora seeds was compared with a standard drug glibenclamide (600 µg/ kg body wt/ day p.o.).
KEYWORDS: Alloxan model of hyperglycemia – Cholic and Deoxycholic acid – Antioxidant enzymes - Lipases - Lecithin cholesterol acyltransferase – Plant antioxidants.
The antidyslipidemic activity of Tinospora cordifolia (T. cordifolia): family Menispermaceae, Hindi name: Giloya; Gurch or Tinospora in english, extract has been studied in alloxan induced diabetic rats. In this model, oral administration of stem extract (500 mg/kg bw. p.o.) for 30 days resulted significant decreased in the levels of blood glucose, plasma lipids and reactivated post heparin lipoprotein lipase activity in alloxan induced diabetic rats. Furthermore, the stem extract (50-500 µg) when tested for its antioxidant activity, shown to inhibit the generation of super oxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems in vitro. The results of the present study demonstrated antidyslipidemic and antioxidant activities of Tinospora cordifolia which could help in prevention of diabetic- dyslipidemia and related complications.
KEY WORDS
Tinospora cordifolia, Anti-dyslipidemic agent, Natural Antioxidants, Post heparin lipolytic activity, Alloxan induced-diabetes. Hypoglycemic agent.