Bio web of conferences/BIO web of conferences, 2024
Vaccination is one of the methods of preventing SARS-Cov-2 infection. Although the efficacy of se... more Vaccination is one of the methods of preventing SARS-Cov-2 infection. Although the efficacy of several vaccines has been observed, it is unknown how long the antibodies remain in the body. The purpose of this study was to examine the antibody titers in adults, 18 months after receiving two doses of the Astra Zeneca vaccine. This is a cross-sectional study with adult subjects who received two doses of the Astra Zeneca vaccine in the Bogor area, conducted in 2023. To evaluate antibody titers (IgG) in serum specimens, the Chemiluminescent Microparticle Immunoassay (CMIA) method was utilized. The antibody titer ≥ 50.0 AU/mL was considered positive. The characteristics 122 subjects were mostly: female (63,1%), 36-45 years old, senior high school graduate (45.9%), unemployed (62,3%), had hypertension history (44.7%), and had no COVID-19 infection during the last six months. The results demonstrate that all the subjects had a positive SARS Cov-2 antibody titer, with a range titer of 87 -80260 AU/mL and a geometric mean titer of 3246.4 AU/mL. In conclusion, this study found that the two doses of the Astra Zeneca vaccine made a significant contribution to the acquisition of anti-SARS-CoV-2S antibodies in adults.
Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022), 2023
Hospital is a place with the highest risk of Covid-19 transmission. Healthcare workers should app... more Hospital is a place with the highest risk of Covid-19 transmission. Healthcare workers should apply safety precautions in order to avoid infection. This study was conducted to obtain information about hospital loci (rooms) that are at risk of transmission of the SARSCov-2 virus. Six hospitals located in DKI Jakarta, East Java, West Java, and North Sumatra provinces were chosen as study locations. Surface swab specimens from rooms and objects that were potential as a source of transmission, based on WHO recommendations were collected. The RNA virus was detected using RT-qPCR, with N1, and N2 genes (CDC) as primers, also the RNP gene as a control. All 556 surface swab samples were retrieved on the first week of December 2020, taken from 14 types of rooms consisting of 67 rooms. The distribution of genetic material of the SARS-CoV-2 virus was mostly obtained in the ICU by 60% (3/5), followed by patient rooms, patient bathrooms, and ER, each 30% (2/6) respectively. Based on the number of specimens, the ICU rooms have also the highest proportion of positive results (12,77% of 47), then the ERs (9.8% of 51). The RNA virus was detected in oximeters, ventilation/air filters, floors, walls, beddings, IV poles, call buttons, garbage bins, tables, lavatory flush buttons, and sinks. In conclusion, ICUs, ERs, patient rooms, and patient bathrooms were sites where the RNA virus was mostly obtained in hospitals, especially in ICUs for Covid-19 patients. It is recommended that hospitals should improve measures to contain the virus and protect healthcare workers, including engineering controls, providing adequate personal protective equipment and maintaining health protocols.
Background. Hepatitis B is a viral infection that has a high prevalence in Indonesia. The Ministr... more Background. Hepatitis B is a viral infection that has a high prevalence in Indonesia. The Ministry of Health of Indonesia has conducted a national vaccination program for hepatitis B. In order to evaluate the success of the hepatitis B vaccination in Indonesia, a community study based on basic health research (Riskesdas) was performed nationwide since 2007 for five year period in 2007, 2013, and 2018. Methods. Further statistical analysis was performed specifically for the children under 59 months old (toddlers) immunized in both urban and rural areas in 2007, 2013, and 2018 based on certain characteristics by examining antibodies against HBsAg (anti-HBs), IgG antibodies against the core antigen (HBcAb), surface antigen (HBsAg) of hepatitis B virus (HBV). The data obtained from the data management laboratory of Ministry of Health, Indonesia, was analyzed with Bivariate analysis with continuity correction chi-square or Pearson chi-square using Stata software version 16. Results. This study showed an increase in hepatitis B coverage of complete immunization (30% in 2007, 60.3% in 2013, and 57% in 2018), which was also influenced by mothers' level of education (Pearson chi-square , p < 0.05) and access to health service points within 30 minutes (OR = 1.3-2.8, p < 0.05). The trend of the percentage of immune status (anti-HBs) was increased (41.8% in 2007; 56.1% in 2013; and 79.1% in 2018). The higher anti-HBs was found in complete hepatitis B immunization status (OR = 1.5-2, p < 0.05) and in good nutritional status (p < 0.05). However, the anti-HBs was found decreased with increasing age (p < 0.05). The trend of positive HBcAb (exposure to HBV infection) showed a decrease gradually of almost ten times from 2007 (8.6%-13.5%) compared to 2013 (2.6%-11.1%) and 2018 (1.1%-2%). Urban areas were at higher risk of hepatitis B exposure (OR = 1.4-2.2) than rural areas (OR = 0.37-0.80). The HBsAg data were only available in 2013 and 2018. Riskesdas data analysis showed the prevalence of hepatitis B (HBsAg) was lower in complete immunization status than that in incomplete one (p < 0.05), but with an increase from 3.9% (2013) to 9.3% (2018), possibly due to inappropriate implementation of birth dose immunization or a vaccine-escape mutant from the HBV variants.
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Dec 1, 2022
Diabetes mellitus is one of metabolic diseases characterized by the elevated blood glucose levels... more Diabetes mellitus is one of metabolic diseases characterized by the elevated blood glucose levels. There are growing studies regarding the effect of diabetes to the development of other diseases such as hypertension, stroke, and even blindness. In the study performed by Dzięgielewska-Gęsiak et al, the comparison of two different treatments in diabetic type-2 patients regarding the effect to the microbiota composition was shown and discussed. Their study compared the microbiota profile in patients treated by metformin only and metformin supplemented with insulin. This study is an interesting and informative study, which gives a novel idea and hint of the different pattern of microbiota composition as the effect of different treatments given to patients. However, certain suggestions are given to the authors as consideration for their future studies. The alteration of homeostatic gut microbiota composition has been continuously reported to play important role in the health field, especially in metabolic diseases, such as diabetes mellitus. 1 The study performed by Dzięgielewska-Gęsiak, et al, compared the profile of gut microbiota of the patients with diabetes mellitus type-2 given by two different treatments, metformin or metformin and insulin. 2 Their results showed that there were no significant differences in the gut microbiota profile detected and analyzed from the patients treated with metformin compared to those treated with metformin and insulin. A question addressed here was why and how? Were the patients included in this study maintaining significant diet and life circumstances for that long time? Does insulin as a hormonal agent really do nothing in the gut microbiota alteration? Meanwhile, the microbiota composition was reported as one of the essential aspects in the development of metabolic diseases and one of factors causing the metabolic disorders itself, in which diabetes mellitus is included. 3 After a deep reading, we provided several suggestions for the authors of this study or even for other scientists in the field as worth considering suggestions for being hopefully unraveled in the future studies. The long term treatment with metformin only or metformin+insulin, based on this particular study, caused no significant effect on the gut microbiota profile of the patients whom got the treatment for more than five years. However, there could be significant differences in gut microbiota profile if being compared to patients whom were just recently being treated with metformin. The comparison of gut microbiota profile could have been performed in comparing those in patients treated with metformin and other different anti-diabetic agent (treatment). The abundant bacteria found in the undertreatment type-2 diabetic patients were Clostridiales, Firmicutes and Verrucomicrobia. Meanwhile, Bacteroidetes and Proteobacteria were in the smaller total proportions. In previous study, Bacteroidetes was reported to be associated with the high risk of diabetes mellitus while being compared to the
Actualmente las ontologías son consideradas uno de los pilares de la web semántica por todas las ... more Actualmente las ontologías son consideradas uno de los pilares de la web semántica por todas las ventajas que ellas pueden ofrecer en la representación formal del conocimiento. Paralelamente a esto, a medida que el campo de las ontologías se va desarrollando, la cantidad de usuarios no expertos en lenguajes de etiquetado va creciendo. Surge entonces la necesidad de contar con herramientas automatizadas que permitan una sencilla interacción con el usuario a fin de facilitar la creación y diseño de ontologías de propósito específico. En este trabajo se presenta OntoACTION, una herramienta de software que permita al usuario definir ontologías activas mediante grafos o mediante lenguajes de etiquetado, traduciéndolas al conjunto de axiomas procesables por una máquina razonadora de ontologías ACTION. Para probar la herramienta se especificó de forma gráfica y textual el dominio de los fenómenos climatológicos dado por el Servicio de
Kejadian Infeksi Ulang Virus Dengue Berdasar Respon Imun Humoralnya Pada Penderita DBD DI Dua Rumah Sakit DI Dki Jakarta
Demam Berdarah Dengue (DBD) merupakan penyakit endemik di Indonesia. Upaya pencegahan dengan imun... more Demam Berdarah Dengue (DBD) merupakan penyakit endemik di Indonesia. Upaya pencegahan dengan imunisasi Dengue menghadapi beberapa kendala. Dewasa ini telah dilakukan penelitian dan pengembangan propfilaksi DBD di beberapa negara di Asia. Penelitian ini mencoba memberikan masukan mengenai pola infeksi ulang virus Dengue pada penderita DBD di dua RS di Jakarta. Telah diperiksa sebanyak 49 penderita tersangka Demam Berdarah Dengue di RSPI dan RS Pasar Rebo Jakarta. Konfirmasi DBD dilakukan dengan menentukan serokonversi anti Dengue menggunakan Uji Hambatan Hemaglutinasi (HI) terhadap ke empat antigen Dengue, DV-1; DV-2; DV-3 dan DV-4. Selain itu dilakukan uji kualitatif untuk mendeteksi IgM anti Dengue dengan Uji Dengue Strip, Enzyme Immuno Assay (EIA). Hasil pengamatan menunjukkan bahwa dari 49 penderita tersangka DBD ditemukan 19 penderita (38,8%) infeksi primer dan 11 penderita (22,4%) infeksi sekunder DBD; 16,3% presumptif dan sisanya 22,4% seronegatif Dengue. Hasil pemeriksaan ku...
Telah dilakukan, penelitian tentang manifestasi klinik penderita DBD dewasa di Jakarta dan Suraba... more Telah dilakukan, penelitian tentang manifestasi klinik penderita DBD dewasa di Jakarta dan Surabaya, masing-masing di Bagian Penyakit Dalam RS. Persahabatan dan Bagian Penyakit Dalam RS Dr. Sutomo. Tujuan penelitian ini adalah untuk menentukan standart kriteria diagnosa klinik bagi penderita DD/DBD pada orang dewasa. Sebagai patokan diagnosa klinis dipakai kriteria WHO 1985 pada anak untuk menjaring kasus DD/DBD. Sebanyak 206 kasus, masing-masing 123 kasus dari Jakarta dan 83 kasus dari Surabaya diteliti gejala klinis yang timbul dan dibandingkan dengan hasil tes serologi menggunakan IgM capture ELISA. Manifestasi klinik penderita DBD dewasa yang diperoleh menunjukkan: - Tourniquet positif 72.9% - Perdarahan Spontan * Pada gusi 42.6% * Epistaksis 38.5% * Petechie 35.6% - Nyeri perut 50.0% - Nyeri kepala 72.9% - Batuk 35.1% - Muntah 48.7% - Syok 1.6% - Trombositopenia 64.2% Dibandingkan dengan manifestasi klinik DBD anak tidak berbeda jauh, hanya pada DBD dewasa perdarahan umumnya le...
Chikungunya is a re-emerging disease that caused byalphavirus family Togaviridae. Chikungunya als... more Chikungunya is a re-emerging disease that caused byalphavirus family Togaviridae. Chikungunya also can often caused outbreak in Asia, including Indonesia. Disease characterized by fever accompanied by pain in the joints of the dominant. Chikungunya virus is transmitted by mosquitoes, especially Aedes aegypti, A. Albopictus and Mansoniasp. This study was conducted to identify significant clinical symptoms of chikungunya virus infection. Sampling was carried from Chikungunya outbreak area. Suspected Chikungunya are interviewed and taken blood. Specimens were examined using reverse transcription - polymerase chain reactions (RT-PCR). Interviews were conducted on111suspectedchikungunya from 6 provinces.Clinical symptoms analisys was performed from 28 confirm chikungunya cases based on laboratory result. Chikungunyashowedmore onthe malesex, and the productive age group (64.3%). The most joint pain symptom reported is wristand knee joints (92.9%), followed by the ankle joint (75%) and fin...
Study Epidemiologi Molekuler Virus Chikungunya Penyebab Kejadian Luar Biasa (KLB) Di Indonesia Tahun 2011
Penelitian epidemiologi virus chikungunya dilaksanakan tahun 2011, sesuai dengan judul dari penel... more Penelitian epidemiologi virus chikungunya dilaksanakan tahun 2011, sesuai dengan judul dari penelitian bahwa responden yang akan dipilih adalah didaerah yang melaporkan adanya Kejadian Luar Biasa, sepanjang tahun 2011 sebanyak 6 provinsi yang mempunyai kasus dimana saat pemberitahuan dilapangan masih ada klinis chikungunya. Dari ke enam provinsi diperoleh responden sebanyak 111 orang yang merupakan klinis chikungunya. Pada pemeriksaan dengan menggunakan PCR terhadap virus chikungunya sebanyak 30 (27%) dari seluruh responden ternyata positive. Gejala klinis berupa sakit pada sendi dialami oleh semua penderita, sendi yang dikeluhkan oleh penderita adalah sendi lutut (93.3%), sendi tangan (90%), sendi pergelangan kaki (73.3%), dan sendi jari tangan (63.3%). Bila dilihat lama demam yang dialami oleh penderita terlihat bahwa 90% positive antara 1 s/d 3 hari, dan sisanya 10% lama demam 4 s/d 7 hari, dan tidak satupun ditemukan positive pada responden yang sudah menderita demam lebih dari ...
Pertussis cases have been reported most frequently in developed countries, but they are predicted... more Pertussis cases have been reported most frequently in developed countries, but they are predicted to be the most prevalent in developing countries. Indonesia, a developing country, routinely conducts case-based surveillance for pertussis. We reviewed the data on pertussis cases and close contacts based on clinical sample documents examined in the National Reference Laboratory for pertussis, Indonesia (2016-2020). Our objective was to analyze the laboratory and epidemiological aspects of pertussis cases and close contacts, particularly to evaluate the implementation of a 5-year case-based surveillance of pertussis in Indonesia. Data were collected from sample documents and annual laboratory reports between January 2016 and December 2020. We analyzed the proportion of pertussis cases and close contacts by geographic region, year, age, and sex. We used the χ 2 test to correlate the laboratory and epidemiological data. In total, 274 clinical cases of pertussis and 491 close contacts were recorded in 15 provinces. The peak number of cases occurred in 2019, with a positivity rate (percentage of laboratory-confirmed cases) of 41.23% (47/114). Clinical cases were dominated by infants aged <1 year (55.5%), and 52.9% of them were aged <6 months. Similarly, 72.3% (68/94) of the laboratory-confirmed cases were infants. Both clinical cases and positivity rates tended to be higher in females (155 cases, 38.1%) than in males (119 cases, 29.4%). No confirmed cases were found in children aged �10 years, although positive results still occurred in close contact. Age-group and laboratory-confirmed cases were correlated (p = 0.00). Clinical and confirmed cases of pertussis occurred mostly in the early age group and may be lower in those aged �10 years, especially in confirmed cases. New policies
Infeksi Japanese enchepahalitis pada Babi di beberapa Provinsi Indonesia Tahun 2012 (Japanese enchephalitis Infection in Pigs in Some Provinces, Indonesia in 2012
The treatments of diphtheria were done by giving Anti-diphtheria serum (ADS) and antibiotics. Pen... more The treatments of diphtheria were done by giving Anti-diphtheria serum (ADS) and antibiotics. Penicillin and eritromisin are drugs of choice were recommended for cases and contacts of diphtheria. The presence of antimicrobial resistance against C.diphtheriae will increase the problem of eradication, prevention and treatment of diphtheria.Meanwhile the selection of sensitivity test methods must be considerated with the laboratory conditions. The aims of study to determine the pattern of resistance and effectiveness several antibiotics againts to C. diphtheriae and compare two antibiotic sensitivity test methods, between disc immunodiffusion and minimum inhibitory concentration (MIC). Determination of two sensitivity test methods were conducted by using diffusion disk agar with medium Muhler hilton agar by addition of 2-5% sheep blood. A total of 57 diphtheria isolates showed by MIC E-test methods were sensitive to ciprofloxacin, rifampicin, gentamicin and linezolid. Then all ciprofloxacin and linezolid showed sensitive against to C,diphtheriae by agar disc diffusion method. A quite high resistance was found 84.2% tetracycline for agar disc diffusion method and 77.2% for MIC E-test method. Furthermore, the drugs programs of C. diphtheriae infection showed 10.5% penicillin and 5.3% erythromycin resistant by agar diffusion disc method. Then 7% penicillin and 3,5% Erythromycin showed resistance by the MIC E-test methods. Both of those methods have agreement between 82.0%-100% depending on the antibiotic agents.
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