Papers by Ivone Giffard Mena
Unraveling the gut microbiota of Mexican pinnipeds: the dominance of life histories over phylogeny
Applied and environmental microbiology, May 21, 2024
Bacterial and viral co-infections in aquaculture under climate warming: co-evolutionary implications, diagnosis, and treatment
Diseases of aquatic organisms, Apr 11, 2024
Marine Genomics, 2018
The Crassostrea virginica oyster has biological and economic importance in the Gulf of Mexico, an... more The Crassostrea virginica oyster has biological and economic importance in the Gulf of Mexico, an area with a high extraction and production of hydrocarbons. Exposure to hydrocarbons affects the reproductive processes in bivalves. In C. virginica, the effect of hydrocarbons on the gonad of the undifferentiated organism has not been evaluated to determine the possible damage during the maturation process. To evaluate this effect, RNA-seq data was generated from C. virginica gonads exposed to a 200 μg/L of hydrocarbons at different exposure times (7, 14 and 21 days) and a control treatment (without hydrocarbons). The analysis of the gonad transcriptome showed the negative effect of hydrocarbons on maturation, with a sub-expression of 22 genes involved in different stages of this process. Additionally, genes in the immune system were down-regulated, which may indicate that exposure to hydrocarbons causes immunosuppression in bivalves. A group of oxidative stress genes was also reduced. These data contribute to a better understanding of the effect of hydrocarbons on the reproductive process in bivalves and, at the same time, allow us to identify possible biomarkers associated with hydrocarbon contamination in the gonad of C. virginica.
Metabarcoding Used for the First Time to Identify Prey of Wild Totoaba macdonaldi
Integrative And Comparative Biology
Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploita... more Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploitation resulted in the Mexican government banning the fishing of this species in 1975, and it being listed as endangered. However, the species is still subject to illegal fishing. Despite its conservation status, little is known about totoaba biology. The present study aimed to implement, for the first time, a metabarcoding protocol to describe the totoaba diet. Four wild totoaba individuals, seized by Mexican law enforcement agents, were dissected, and their stomach contents were collected. Three representative amplicon libraries were generated for cephalopods, chordates, and eukaryotes. After sequencing, 18 different taxa were identified, of which 11 species were recognized as prey. The totoaba were found to have consumed Pacific anchovy (Cetengraulis mysticetus), flathead grey mullet (Mugil cephalus), bigeye croaker (Micropogonias megalops), northern anchovy (Engraulis mordax), ocean wh...
White Spot Syndrome Virus (WSSV) infects several economically important aquacultural species, cau... more White Spot Syndrome Virus (WSSV) infects several economically important aquacultural species, causing significant losses to the industry. This virus belongs to the Nimaviridae family, and has a dsDNA genome ranging from 257 to 309 kb (more than 20 isolate genomes fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. In particular, Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with gain or loss of virulence.The pathogenicity of three isolates from three locations in Mexico (two from Sinaloa: 'CIAD', and 'Angostura', and one from Sonora: 'Sonora') was evaluated in vivo in white shrimp (P. vannamei) infection assays. Differences were observed in the mortality rate of shrimp among the three isolates, with the Sonora isolate being the most virulent. Subsequently, ...
Metabarcoding Used for the First Time to Identify Prey of Wild <i>Totoaba macdonaldi</i>
Integrative and Comparative Biology, May 10, 2023
Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploita... more Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploitation resulted in the Mexican government banning the fishing of this species in 1975, and it being listed as endangered. However, the species is still subject to illegal fishing. Despite its conservation status, little is known about totoaba biology. The present study aimed to implement, for the first time, a metabarcoding protocol to describe the totoaba diet. Four wild totoaba individuals, seized by Mexican law enforcement agents, were dissected, and their stomach contents were collected. Three representative amplicon libraries were generated for cephalopods, chordates, and eukaryotes. After sequencing, 18 different taxa were identified, of which 11 species were recognized as prey. The totoaba were found to have consumed Pacific anchovy (Cetengraulis mysticetus), flathead grey mullet (Mugil cephalus), bigeye croaker (Micropogonias megalops), northern anchovy (Engraulis mordax), ocean whitefish (Caulolatilus princeps), milkfish (Chanos chanos), and Pacific sardine (Sardinops sagax). Members of the Euphausiidae family (krill) were also identified. This study identified up to four times more species in much fewer samples than previous studies based on morphological recognition, thus confirming metabarcoding as an effective method for studying the feeding habits of this species and one providing the tools required for further analysis of the totoaba diet.
Aquaculture and Fisheries
Melanization of shrimp gills is a general symptom in shrimp black bill diseases (sBG); therefore,... more Melanization of shrimp gills is a general symptom in shrimp black bill diseases (sBG); therefore, the cause is not immediately apparent. This study aimed to determine the primary and secondary agents associated with a black gill outbreak in the Delvar complex in the Bushehr Province of Iran. During July and August of 2020, 450 healthy and affected shrimps were collected based on brown to black gill color from three farms of the Bushehr Province in Iran. Characterization of pathogens was performed by gross signs, wet mounts, microbiological tests, histopathology methods, and sequencing of molecular markers. The mean weight and length of infected shrimps was significantly (P &lt; 0.05) lower than the healthy shrimps. The histological examination of sBG indicated the adhesion of secondary lamellae, accumulation of hemocyte cells at the end of the secondary lamellae, and necrosis of epithelial cells with pyknosis and karyorrhexis. According to our results, Aspergillus sydowii as a primary agent correlates with sBG disease in Litopenaeus vannamei from Iran, and other biotic factors, including bacterial (Vibrio owensii and V. parahaemolyticus) probably are secondary agents associated with sBG disease.
Ontogenetical development of branchial chambers of Litopenaeus vannamei (Boone, 1931) and their involvement in osmoregulation: ionocytes and Na+/K+-ATPase
Cell and Tissue Research, Sep 9, 2022
Incidencia de BACTERIAS LUMINISCENTES en cultivos larvarios de Penaeus stylirostris
CICIMAR Oceánides, 2018
Some microalgae are recognized for producing pigments and other metabolites with biotechnological... more Some microalgae are recognized for producing pigments and other metabolites with biotechnological importance, particularly, Dunaliella salina is a remarkable one. These kind of compounds are used as food and have a great industrial potential. The pigment industry comprises a millionaire market value, being β-carotene one of the most profitable one. In this study we describe the morphology, molecular identification, growth dynamics, proximal composition, nutrients and pigment content of a recently isolated Dunaliella salina strain (SQ) under different salinity/light conditions, in order to highlight its remarkable properties for biotech/biomed industry. D. salina SQ reached the highest densities (1.07-1.25 cell mL-1 x106) at low salinities (100-500 mM NaCl) under continuous light regimen (24:0 h Light:Dark). Neoxanthin (Neo) and violaxanthin (Viol) were the most abundant pigments when exposed to 500 mM NaCl (18:6 h Light:Dark). Furthermore, this peculiar strain produces other compoun...
Beilstein Journal of Organic Chemistry, 2021
The white spot syndrome virus (WSSV), currently affecting cultured shrimp, causes substantial eco... more The white spot syndrome virus (WSSV), currently affecting cultured shrimp, causes substantial economic losses to the worldwide shrimp industry. An antiviral therapy using double-stranded RNA interference (dsRNAi) by intramuscular injection (IM) has proven the most effective shrimp protection against WSSV. However, IM treatment is still not viable for shrimp farms. The challenge is to develop an efficient oral delivery system that manages to avoid the degradation of antiviral RNA molecules. The present work demonstrates that VLPs (virus-like particles) allow efficient delivery of dsRNAi as antiviral therapy in shrimp. In particular, VLPs derived from a virus that infects plants, such as cowpea chlorotic mottle virus (CCMV), in which the capsid protein (CP) encapsidates the dsRNA of 563 bp, are shown to silence the WSSV glycoprotein VP28 (dsRNAvp28). In experimental challenges in vivo, the VLPs- dsRNAvp28 protect shrimp against WSSV up to 40% by oral administration and 100% by IM. The...
Journal of Aquaculture & Marine Biology, 2021
In this short review, we highlight the importance of combining cell culture and nextgeneration se... more In this short review, we highlight the importance of combining cell culture and nextgeneration sequencing for the study of viruses in aquaculture. Moreover, we summarize some key examples of previously published studies that have implemented this approach and discuss the advantages of nanopore sequencing and other long-read sequencing technologies. With the rapid advances of genomic research, selection of the best tool to carry out analyses, it's a computational challenge, but the potential for their applications is enormous. Therefore, this mini-review highlights important NGS bioinformatic tools and recent studies in cell culture for the study of aquatic virology.
White spot syndrome virus (WSSV) has a worldwide distribution and is considered one of the most p... more White spot syndrome virus (WSSV) has a worldwide distribution and is considered one of the most pathogenic and devastating viruses to the shrimp industry. A few studies have explored the effect of WSSV on shrimp acclimated to low (5 practical salinity units [psu]) or high (>40 psu) salinity conditions. In this work, we analysed the physiological response of WSSV-infected Litopenaeus vannamei juveniles that were acclimated to different salinities (5, 15, 28, 34 and 54 psu). We evaluated the osmotic response and survival of the shrimp at different times after infection (0 to 48 hours), and we followed the expression levels of a viral gene (vp664) in shrimp haemolymph using real-time PCR. Our results indicate that the susceptibility of the shrimp to the virus increased at extreme salinities (5 and 54 psu), with higher survival rates at 15 and 28 psu, which were closer to the iso-osmotic point (24.7 psu, 727.5 mOsmol/kg). Acute exposure to the virus made the haemolymph less hyperosmotic at 5 and 15 psu and less hypo-osmotic at higher salinities (>28 psu). The capacity of white shrimp to osmoregulate, and thus survive, significantly decreased following WSSV infection. According to our results, extreme salinities (5 or 54 psu) are more harmful than seawater.
Ontogenèse De L'Adaptation À La Salinité Chez Le Téléostéen Dicentrarchus Labrax : Rôle Du Tube Digestif Dans La Balance Hydro-Minérale
L'implication du tube digestif (TD) dans l'osmoregulation a ete etudiee durant le develop... more L'implication du tube digestif (TD) dans l'osmoregulation a ete etudiee durant le developpement du loup (bar) Dicentrarchus labrax. Differents niveaux d'integration (genes, proteines, cellule, physiologie, comportement) ont ete pris en compte, en liaison avec l'ecologie, dans une approche ecophysiologique. L'expression et la localisation des proteines impliquees dans la balance ionique et hydrique (ATPase Na+/K+, aquaporines AQP) ont ete suivies par histologie, microscopie electronique, immunofluorescence et biologie moleculaire. Les AQP1 et AQP3 ont ete sequencees et caracterisees. L'ontogenese du TD a ete decrite. Fonctionnel des l'eclosion, son implication dans l'osmoregulation s'accroit avec l'augmentation de la capacite osmoregulatrice a la transition metamorphique larve/juvenile. La capacite a absorber des ions precede celle de l'absorption de l'eau, en liaison respective avec l'expression de l'ATPase Na+/K+ et d'AQP1. La regulation de l'expression de ces proteines selon la salinite apparait au cours de la metamorphose. Le TD est fortement implique dans l'absorption d'eau compensant la deshydratation en eau de mer. L'exposition a l'eau douce provoque chez les juveniles des mortalites differentielles entre survivants (FWS) et ceux qui y meurent (FWU). Chez les FWU, la densite des tubules urinaires est plus faible dans le rein, ce qui limite la reabsorption ionique, et l'AQP1 est surexprimee dans le TD, entrainant probablement une augmentation de l'invasion d'eau via l'intestin. Ces deux phenomenes sont a l'origine de la faible osmolarite sanguine des FWU, cause de leur mort. Durant l'ontogenese du loup, les regulations combinees effectuees par le TD, le rein et les branchies repondent aux variations de salinite du milieu naturel, influant sur la tolerance a la salinite, et permettant le peuplement de divers milieux, de la mer aux lagunes et estuaires
White spot syndrome virus (WSSV) has a worldwide distribution and is considered one of the most p... more White spot syndrome virus (WSSV) has a worldwide distribution and is considered one of the most pathogenic and devastating viruses to the shrimp industry. A few studies have explored the effect of WSSV on shrimp acclimated to low (5 practical salinity units [psu]) or high ([40 psu) salinity conditions. In this work, we analysed the physiological response of WSSV-infected Litopenaeus vannamei juveniles that were acclimated to different salinities (5, 15, 28, 34 and 54 psu). We evaluated the osmotic response and survival of the shrimp at different times after infection (0 to 48 hours), and we followed the expression levels of a viral gene (vp664) in shrimp haemolymph using real-time PCR. Our results indicate that the susceptibility of the shrimp to the virus increased at extreme salinities (5 and 54 psu), with higher survival rates at 15 and 28 psu, which were closer to the iso-osmotic point (24.7 psu, 727.5 mOsmol/kg). Acute exposure to the virus made the haemolymph less hyperosmotic at 5 and 15 psu and less hypo-osmotic at higher salinities ([28 psu). The capacity of white shrimp to osmoregulate, and thus survive, significantly decreased following WSSV infection. According to our results, extreme salinities (5 or 54 psu) are more harmful than seawater.
Renal dysfunction and water channels in fresh water unadapted European sea-bass (Dicentrarchus labrax)
The Faseb Journal, Mar 1, 2008
Mechanisms of acclimation to hypersalinity in two European sea bass lineages: a focus on the kidney function
Aquaculture
Effects of salinity on survival and plasma osmolarity of Totoaba macdonaldi eggs, larvae, and juveniles
Journal of Experimental Marine Biology and Ecology
Aquaculture Research
Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudd... more Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudden salinity changes in their natural environment, which is more common than the exception, so they must adapt to survive and cope with extreme salt conditions. Therefore, Bullseye puffer juveniles were exposed to short-term stress (39 hr) by fluctuating salinity conditions (41,
Osmoregulation, growth, and survival during the larval development of bullseye puffer fish Sphoeroides annulatus (Jenyns, 1842, Pisces: Tetraodontidae)
Marine and Freshwater Behaviour and Physiology, 2015
By: Pérez-Robles, Javier Diaz, Fernando Denise Re, Ana Giffard-Mena, Ivone Abdo-de la Parra, Marí... more By: Pérez-Robles, Javier Diaz, Fernando Denise Re, Ana Giffard-Mena, Ivone Abdo-de la Parra, María Isabel Ibarra-Castro, Leonardo We analysed the effects of salinity on survival, growth and the osmoregulatory capacity (OC) of puffer fish larvae (Sphoeroides annulatus). In experiment 1, we compared the growth of larvae maintained at a constant temperature (28 ± 0.5 °C) under seven salinity levels (5, 12, 19, 26, 33, 35 and 40 psu) over a 28-day period. No significant differences were found for larval growth. However, salinity significantly affected survival. The larvae hyperosmoregulated at low salinities and hypo-osmoregulated at high salinities. The isosmotic point ranged from 338.9 to 355.9 mOsm kg−1, depending on salinity. In experiment 2, survival increased with increasing salinity following direct exposure to the same seven salinity levels over a 72-h period. The isosmotic point ranged from 256.5 to 466.7 mOsm kg−1 depending on the developmental stage. This species hyper-regulated at 5–12 psu, hypo-regulated at 19–40 psu and demonstrated a high euryhalinity at 7 DAH with 95% survival.
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Papers by Ivone Giffard Mena