Papers by Shailesh K Tiwari
De novo transcriptome assembly of Solanum melongena Ramnagar Giant
Vegetable Science, Dec 28, 2023
The Indian Journal of Agricultural Sciences
Genetic variation among 29 bitter gourd genotypes was assessed using morphological and ISSR marke... more Genetic variation among 29 bitter gourd genotypes was assessed using morphological and ISSR markers during 2013–14. High genetic variability was observed for yield per plant (397–1990 g), number of fruits per plant (9.18–43), individual fruit weight (25.47–125.67 g), plant height (110–503 cm), fruit length (6.39–25.97 cm), fruit diameter (2.53–6.1 cm), number of seeds (5–22.33) and number of branches per plant (4.67–16.44). The pair-wise Jaccard’s similarity coefficient ranged between 0.22–1.00 based on morphological traits. The size of fragments varied from 200 bp (by ISSR marker UBC-808, 811, and 835) to 1550 bp (by ISSR marker UBC-807). Maximum polymorphism was shown by primers UBC-825 and lowest level of polymorphism was shown by UBC-807, UBC-809 followed by UBC-812, UBC-810. On an average 63.16% polymorphism was obtained per primer. All genotypes were grouped into five main clusters. Jaccard’s similarity coefficient ranged from 0.73–1.00. These data re...
Whether Leucinodes orbonalis Guenée is becoming a serious problem to brinjal seedlings in nursery
Pest Management in Horticultural Ecosystems, 2015
Incidences of brinjal shoot and fruit borer, Leucinodes orbonalis were observed in brinjal seedli... more Incidences of brinjal shoot and fruit borer, Leucinodes orbonalis were observed in brinjal seedlings at nursery in and around Varanasi, Uttar Pradesh during August September 2015. Maximum of 37.16% seedlings were damaged in nursery. The affected seedlings drooped down, wilted and finally die prompting the farmers to re-sow the crop. Critical observation revealed that gravid female prefers to lay eggs at the junction of two leaves and the seedlings with minimum stem thickness of 1.65 mm with an average of 1.78±0.13 mm diameter were preferred for egg laying. Histological studies had shown that on damaged stem, larvae fed the cells from pith (parenchyma), vascular bundles (xylem, vascular cambium, phloem and bundle sheath), bundle caps and endodermal cells leaving only the epidermal and hypodermal cells. Prophylactic spraying(s) in the nursery must be adopted before attaining this preferred minimum stem thickness along with other management practices in endemic areas to get rid of fr...
Vegetable Varieties/Hybrids Developed by IIVR and Possibilities of Public-Private Partnerships in Vegetable Seeds
Hetrosis for productivity traits in brinjal (Solanum melongena L.)
Vegetable Science, 2013
Brinjal ( Solanum melongena L.), also known as eggplant, is commercially important solanaceous ve... more Brinjal ( Solanum melongena L.), also known as eggplant, is commercially important solanaceous vegetable crop of Indian subcontinent. Rich variability exist for both cultivated type and its wild species in the country. It is popular among people of all social strata and hence, it is rightly called as vegetable of masses (Patel and Sarnaik, 2003). Hybrid vigour in brinjal was probably first reported by Nagai and Kida (1926) across combinations of some Japanese varieties of brinjal. With increasing popularity of F 1 hybrids in eggplant, it is imperative to obtain hybrids having excellent quality coupled with high yields. To obtain high yield per unit area, exploitation of hybrid vigour is one of the good way and particularly in crop like brinjal, whereas more seeds per fruit are obtained of hybrid brinjal. The common approach of selecting parents on the basis of per se performance does not necessarily lead to fruitful results but it provides to the breeders an insight in to nature and...
Harnessing endophytic microbial diversity for stress alleviation: Current perspectives for crop improvement
Mitigation of Plant Abiotic Stress by Microorganisms
Promotion and protection of ITKs to withstand impacts of climate change on agro-biodiversity and food security
Transplantation Proceedings, 2003
National Journal of Maxillofacial Surgery, 2013
Lichen planus is a relatively common disorder, estimated to effect 0.5% to 2.0%of the general pop... more Lichen planus is a relatively common disorder, estimated to effect 0.5% to 2.0%of the general population. It is a chronic inflammatory disease affecting mucosal and cutanous tissue. Oral lichen planus occurs more frequently than the cutanous form and tends to be more resistant to any treatment.
Long-term field performance of micropropogated Aloe vera plants
Long-term field performance of micropropagated, primary (tissue cultured plants) and secondary (s... more Long-term field performance of micropropagated, primary (tissue cultured plants) and secondary (suckers produced by tissue cultured plants) plants of Aloe vera plants was compared with control (non- tissue cultured plants) plants. The regenerated plants both primary and secondary showed 100 % survival in the field like control plants. Growth of primary tissue cultured plants lagged compared to that of control plants especially on the basis of leaf fresh weight and leaf length. Secondary tissue cultured plants did not show significant difference in growth from the control plants in any of the character evaluated. RAPD markers were used to assess the clonal fidelity of 25 plants. As all the bands produced by these plants were monomorphic, no variation was detected between tissue cultured and control plants.
Stepping into Farmer's field through Public-Private Partnership (PPP): Initiatives at IIVR, Varanasi
Organogenesis from Leaf Tissue of Spondias pinnata (L. f.) Kurz, SEM study and Genetic Fidelity Assessment by ISSR and ScoT
Plant Cell, Tissue and Organ Culture (PCTOC)
In vitro raised plantlets were obtained from nodal tissue through direct organogenesis and they s... more In vitro raised plantlets were obtained from nodal tissue through direct organogenesis and they served as donor plants for the collection of leaf explants. Leaf explants were inoculated on Murashige and Skoog’s medium with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). Hundred percent callogenesis was observed on medium supplemented with 5 mg l−1 2,4-D. For the multiplication of cells (proliferation), calli were transferred to either basal medium or media containing different types and concentrations of cytokinins. Proliferation was observed maximum on media containing 0.5 mg l−1 BAP or 1.0 mg l−1 BAP. Shoot differentiation from calli took place on media supplemented with BAP in combinations with TDZ or ZN. Initiation of organogenesis was observed in calli within two weeks of their subculture on differentiation medium. Shoot differentiation was maximum, when calli proliferated on medium having 1 mg l−1 BAP were transferred to the medium containing 1 mg l−1 BAP and 0.5 mg l−1 TDZ. Organogenic responses after four weeks of subculture on above differentiation medium were as such: number of shoots per explants (25.33 ± 0.88), number of shoots per calli replicate (3.67 ± 0.33) and maximum shoot length (3.43 ± 0.20 cm). Medium supplemented with 2.5 mg l−1 NAA was most responsive for rooting of shoots (54.16 ± 1.39%). About 62.5% plantlets survived after hardening and 54.17% plantlets got acclimatized. All acclimatized plants were transferred to field condition successfully. Formation of unipolar shoots and their multicellular attachment with callus were observed by scanning electron microscopy. To confirm the genetic fidelity of micropropagated plants, five micropropagated plants derived from different leaf explants and two mother plants (randomly selected from micropropagated plants raised from nodal explants) were subjected to molecular analysis. The genetic fidelity of in vitro regenerated plants was assessed by using SCoT and ISSR molecular markers. 12.5% polymorphism was reported in both studies, which may be due to callus mediated regeneration of shoots. Indirect organogenesis from leaf tissues of Spondias pinnata can be used both for conservation as well as the improvement of plants.
Plant Cell, Tissue and Organ Culture (PCTOC), 2022
An efficient encapsulation and germplasm conservation protocol were developed for Nyctanthes arbo... more An efficient encapsulation and germplasm conservation protocol were developed for Nyctanthes arbor-tristis L. In this study the gel matrix containing three percent sodium alginate (SA) and 100 mM calcium chloride (CaCl2 . 2H2O) was found best for the formation of encapsulated seeds from node explant of this economically valuable species. The viability of encapsulated seeds and shoot sprouting potential was optimized. Encapsulated seeds stored at 4ºC and 24 ºC maintained its viability up to 90 days and showed sprouting potential 42.89±6.04 and 33.53±7.15 percent respectively. Node explant maintain under slow growth condition up to 180 days on one-eighth (1/8 th ) strength MS medium supplemented with 0.5 percent sucrose found suitable to maintain high span viability percent (40.28±2.04) with average number of shoots/ node (1.61±0.28) and shoots length (1.12±0.32 cm) respectively. One-eighth (1/8 th ) strength MS medium supplemented with 0.5 percent sucrose and enriched with 0.5 mg/l abscisic acid (ABA) prolonged the viability up to 40.36±1.01 percent of explant. The best rooting response was achieved on half (½) strength MS medium enriched with 4 mg/l indole-3-acetic acid (IAA). The rooted plant shows 65 percent survivability in open field condition. The true-to-type clonal fidelity assessment of tissue culture recovered acclimated plants with start codon targeted (SCoT) primer profile shows same banding mobility patterns as with source parent mother plant. The maximum banding profile is monomorphic and consistent. Hence on this basis it confirmed the true-to-type clonal stability among them. The protocols display the novel method for conservation of this species under in-vitro condition and facilitate easy exchange of plant germplasm.
Ethnobotanical importance of brinjal in India
Indian horticulture, Sep 29, 2020
Biology of Stem Borer Euzophera Perticella Lepidoptera Pyralidae and Association of Endoparasitoid Pristomerus Euzopherae Hymenoptera Ichneumonidae in Grafted and Ratoon Brinjal Crop
Indian Council of Agricultural Research, Jun 12, 2017
The present study envisages the bionomics of brinjal stem borer, Euzophera perticella on eggplant... more The present study envisages the bionomics of brinjal stem borer, Euzophera perticella on eggplant and occurrence of its prominent endoparasitoid, Pristomerus euzopherae under Varanasi, Uttar Pradesh condition. Affected plants can easily be visualized by their appearance as light yellow in colour, completely drooped, withered and wilted with stunted growth, reduced plant vigour, lowered fruit bearing capacity and detected in patches across the plot. Damage is more severe in near maturing, ratoon and grafted brinjal crop. Its incidence was observed from the second fortnight of February onwards (13.5% stem damage) which increased gradually with 29.75, 51.5, 76.5 and 89.7% stem damage during March, April, May and June, respectively and where almost all the plants were affected by this borer during July. Oviposition, incubation, larval and pupal periods of E. perticella ranged from 4-11, 3-9, 29-47 and 7-14 days, respectively, whereas adult longevity were 4-8 days for male and 5-13 days for females. An Ichneumonid endoparasitoid, Pristomerus euzopherae was recorded associated with this stem borer. Incidence of this parasitoid was noted during second fortnight of April (1.91% parasitization) with highest parasitization (12.48%) during July followed by June (7.73%).
De novo transcriptome assembly of Solanum incanum L
Antioxidant and phytochemical levels and their interrelation in stem and leaf extract of water spinach (Ipomea aquatica)
Indian Journal of Agricultural Sciences, 2016
An investigation was carried out to evaluate nutritional and anti-oxidant properties of leaf and ... more An investigation was carried out to evaluate nutritional and anti-oxidant properties of leaf and stem extracts from 10 genotypes of water spinach (Ipomea aquatica Forsk). The extracts were analyzed in terms for protein, sugar, chlorophyll, carotenoid, phenol, proline, flavonoids and ascorbic acid contents. The analysis revealed a significant variation in the level of protein (10.4-151.6 mg/g FW), sugar (0.33-2.98 g/100g FW), chlorophyll (2.50-4.98 mg/g FW), phenol (0.75-2.11 mg/g FW), proline (0.14-36.14 μg/g FW), carotenoid (0.85-0.1.59 mg/g FW), flavonoid (0.1-0.431 mg/g FW) and ascorbic acid (0.21-1.03 g/100g FW) in leaf extract and (protein 13.1-144.8 mg/g FW, sugar 0.43-3.55 g/100g FW, chlorophyll 0.32-0.76 mg/g FW, phenol 0.54-4.76 mg/g FW, proline 0.09-29.46 μg/g FW, carotenoid 0.12-0.29 mg/g FW, flavonoid 0.05-0.231 mg/g FW and ascorbic acid 0.28-0.62 g/100g FW) in stem extract of the genotypes. The results indicate that total sugar, proline and protein in both stem and leaf...
LTR retroelement in genes related to abiotic stress in Capsicum annuum L
Vegetable Science, 2017
Long terminal repeats (LTRs) are a type of retrotransposons possessing all commonly found structu... more Long terminal repeats (LTRs) are a type of retrotransposons possessing all commonly found structural features of long interspersed element (LINEs), including RNA polymerase III promoter, polyA tail, and flanking repeats. Here we report identification and characterization of LTR element in abiotic stress genes of solanaceae family along with an attempt to explain their functional importance through their secondary structure. We retrieved 366 abiotic stress genes of 5 species of solanaceae family-Solanum lycopersicum, S. melongena, S. tuberosum, Nicotiana tabacum and Capsicum annuum from NCBI nucleotide Database. C. annuum, accession HI543260.1 with the gene Id gi|311377312 possesses the LTR region. The gene reportedly has role in stress tolerance and high yield production. In gene masking, 16 simple repeats were detected, which best masked 569 bp out of 308042 bp, however, in protein masking 1 LTR (Ty1/copia) of 83 bp, 17 simple repeats of 603 bp and 15 low complexity regions of 514 ...
polymorphism detection, as they detected 84.26% as compared to 76.54% for ISSR markers. But, reso... more polymorphism detection, as they detected 84.26% as compared to 76.54% for ISSR markers. But, resolving power (Rp), average bands per primer, Nei’s genetic diversity (h), Shannon’s Information Index (I), total genotype diversity among population (Ht), within population diversity (Hs) and gene flow (Nm) estimates were more for ISSR (7.098, 5.79, 0.245, 0.374, 0.244, 0.137 and 0.635, respectively) as compared to RAPD markers (5.669, 5.35, 0.225, 0.359, 0.225, 0.115 and 0.518, respectively). The regression test between the two Nei’s genetic diversity indexes gave r 2 = 0.3318, showing low regression between RAPD and ISSR based similarities. Regression value for ISSR and ISSR + RAPD combined data is moderate (0.6027), while it is maximum for RAPD and ISSR+RAPD based similarities (0.9125). Thus both the markers are equally important for genetic diversity analysis in Jatropha curcas. Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrogram were compared, ...
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Papers by Shailesh K Tiwari