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Effect of enrichment of organic selenium, inorganic selenium and vitamin E in quail ration on the performances and potency of quail egg as a source of antioxidant Akil, Syahrir; Piliang, Wiranda Gentini; Hanny Wijaya, C.; Utomo, Desianto Budi; Wiryawan, I Komang Gede
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.156 KB) | DOI: 10.14334/jitv.v14i1.357

Abstract

The change of life style influences human health and it contributes to many human diseases outbreak, therefore nutrition of antioxidant is required. This study was aimed to get an optimum level of combined organic selenium, inorganic selenium and vitamin E in obtaining the best production and reproduction of quails and the highest antioxidant level in quail eggs. This study was conducted from January to August 2008.  Numbers of observed quails were 720 individuals (360 female and 360 male quails). The treatments were applied when the quails were six weeks old. Nine treatment diets were: To (commercial diet), T1 (0.46 ppm inorganic Se + 43.50 ppm vitamin E), T2 (0.46 ppm inorganic Se + 87.00 ppm vitamin E), T3 (0.92 ppm inorganic Se + 43.50 ppm vitamin E), T4 (0.92 ppm inorganic Se +87.00 ppm vitamin E), T5 (0.46 ppm organic Se + 43.50 ppm vitamin E), T6 (0.46 ppm organic Se + 87.00 ppm vitamin E), T7 (0.92 ppm organic Se + 43.50 vitamin E) and T8 (0.92 ppm organic Se 0.92 + 87.00 ppm vitamin E). The design of the experimental applied was a factorial – nested design. Any significant differences among the treatment diets were analysed using Duncans test. The result of this study indicated that treatment T7 (0.92 ppm organic selenium + 43.50 ppm vitamin E) in general gave the highest content of selenium in meat, in egg albumin, egg yolk, vitamin E in egg yolk, glutathione peroxidase (GSH-Px), activity of antioxidant, hatchability and low mortality. Key Words: Quail, Selenium, Vitamin E, Antioxidant
The effect of passion fruit hulls level (Passiflora edulis Sims f. edulis Deg) as Kacang goat feed component: I. Intake digestibility and nitrogen retention Simanihuruk, Kiston; Wiryawan, Komang G; Ginting, Simon P
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.742 KB) | DOI: 10.14334/jitv.v11i2.513

Abstract

To study the effect of utilization of passion fruit hulls (Passiflora edulis Sims f. edulis Deg) as feed component on consumption, digestion and nitrogen retention in kacang goats, a trial was conducted using 20 young kacang goats (average initial body weight 23.73±2.16 kg). The experiment was arranged in completely randomized design consisting of 4 diets and 5 replications. Animal were randomly allocated into 4 diets (0, 15, 30, 45% of passion fruit hulls). Each diet contains 2550 Kcal/kg metabolism energy and 14% crude protein. The feeding level was set at 3.8% of body weight based on dry matter. The results of the experiment showed that average dry matter intake, nutrient digestibility, and nitrogen retention were not affected by level of passion fruit hulls (P>0.05), although nutrient digestibility and nitrogen retention tended to decrease with the increasing level of passion fruit hulls. It was concluded that passion fruit hulls can be used till 45% level in the diet. Key Words: Passion Fruit, Consumption, Digestibility, Nitrogen Retention, Kacang Goat
Isolasi Bakteri Pendegradasi Mimosin Asal Rumen Sapi dan Domba yang Diberi Daun Lamtoro dan Pengaruhnya Pada Karakteristik Fermentasi In Vitro Suharti, Sri; Alwi, Windawati; Wiryawan, Komang Gede
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 18, No 1 (2020): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.096 KB) | DOI: 10.20961/sainspet.v18i1.33228

Abstract

Penelitian ini bertujuan untuk mengisolasi bakteri pendegradasi mimosin dari cairan rumen domba dan sapi yang diberi pakan mengandung daun lamtoro dan menganalisis pengaruh inokulasi bakteri pendegradasi mimosin pada karakteristik fermentasi rumen secara in vitro. Tahap pertama adalah isolasi bakteri pendegradasi mimosin dari cairan rumen domba yang diberi pakan mengandung daun lamtoro 30% dan dari rumen sapi Bali di Sumbawa yang diberi pakan daun lamtoro 100%. Tahap kedua adalah inokulasi bakteri pendegradasi mimosin pada fermentasi in vitro dengan substrat mengandung daun lamtoro. Rancangan yang digunakan pada percobaan in vitro adalah rancangan acak kelompok pola faktorial dengan 2 faktor. Pengelompokan berdasarkan waktu pengambilan cairan rumen. Faktor pertama terdiri dari 3 taraf daun lamtoro yaitu 0, 30, dan 60% dalam ransum. Faktor kedua terdiri dari 2 inokulasi pendegradasi mimosin yaitu dengan dan tanpa inokulasi bakteri pendegradasi mimosin. Peubah yang diamati adalah populasi bakteri dan protozoa, nilai pH, konsentrasi NH3, produksi volatile fatty acid (VFA), kecernaan bahan kering (KcBK) dan kecernaan bahan organik (KcBO). Hasil penelitian ditemukan 2 isolat bakteri pendegradasi mimosin dari rumen domba dan 3 isolat rumen sapi Bali. Kelima isolat tersebut mempunyai kemiripan dengan Streptococcus sp. Hasil uji in vitro menunjukkan tidak ada interaksi antara taraf pemberian daun lamtoro dengan inokulasi bakteri pendegradasi mimosin pada fermentasi rumen. Inokulasi bakteri pendegradasi mimosin dapat meningkatkan konsentrasi NH3 dan cenderung memperbaiki produksi VFA dibandingkan dengan perlakuan tanpa inokulasi bakteri. Nilai pH, KcBK dan KcBO tidak berbeda antar perlakuan. Kesimpulan yang dapat diambil adalah inokulasi bakteri pendegradasi mimosin dapat menstimulasi fermentasi rumen secara in vitro.
Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Siti Nur Jannah; Achmad Dinoto; Komang Gede Wiryawan; Iman Rusmana
Media Peternakan Vol. 37 No. 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5594.722 KB) | DOI: 10.5398/medpet.2014.37.3.182

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The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken Fathin Hamida; Komang G Wiryawan; Anja Meryandini
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.879 KB) | DOI: 10.5398/medpet.2015.38.2.138

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Lactic acid bacteria (LAB) regarded as safe microorganisms; they can naturally live in gastrointestinal tract, so appropriately used as a probiotic for chicken. This study aimed to select six isolates of LAB (E1223, E3, E4, E5, E7, and E8) to obtain the isolates potentially as probiotic candidate for chicken. The six isolates were derived from spontaneous fermented corn obtained from Laboratory of Animal Biotechnology and Biomedical, PPSHB, Bogor Agricultural University, Indonesia. LAB isolates were tested their susceptibility to antibiotics (bambermycin, erythromycin, chloramphenicol, and tetracycline) then were examined in vitro for their tolerance to gastrointestinal pH (2, 3, 4, and 7.2) and 0.5% bile salt condition, antimicrobial activity against Salmonella enteritidis and Enterococcus casseliflavus, and ability to adhere to chicken ileal cells. The results showed the isolates E5, E7, and E8 were sensitive to tetracycline and chloramphenicol, they could survive at pH 2, 3, 4, and 7.2, could survive at 0.5% bile salts, produced antimicrobial activity, and able to adhere to ileal cells (9.40±0.00 Log CFU/cm2 of E8) and were significantly (P<0.05) higher than those of control (5.30±0.14 Log CFU/cm2). In conclusion, this study showed that isolate E8 had better potential compared to isolates E5 and E7 in most in vitro assays as a probiotic candidate for chicken. E5, E7, and E8 were closely related with Pediococcus pentosaceus based on 16S rRNA gene.Key words: LAB, probiotic, chicken, in vitro
Fermentation Characteristics and Nitrogen Retention of Madura Cattle Fed Complete Rations Containing Soybean Pod and By-Products Komang Gede Wiryawan; Arief Saefudin; Asnath Maria Fuah; Rudi Priyanto; Lilis Khotijah; Sri Suharti
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.596 KB) | DOI: 10.5398/medpet.2017.40.1.28

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This study was aimed to evaluate the effect of complete rations containing soybean pod and soybean by-products (soybean meal and tofu waste) on rumen microbial population, fermentation characteristics, nutrient digestibility, and nitrogen retention of Madura cattle. Twelve Madura cattle of 1.5 years of age were given 4 feeding treatments in triplicates in randomized block design experiment. The treatments included T0 (100% native grass) as a negative control, T1 (concentrate: grass (60:40) as a positive control, T2 (complete ration containing 15% soybean pods), and T3 (complete ration containing 30% soybean pods). The treatments were based on feeding practices commonly applied by farmers in the village. The results showed that the use of concentrate rations or complete rations containing soybean pod and by-product did not affect protozoa population, ammonia concentration, and total VFA production compared to cattle fed 100% native grass. In contrast, the use of concentrate rations or complete rations containing soybean pod and by-products reduced acetate and increased butyrate proportion compared to native grass. The use of a concentrate ration resulted the highest propionate proportion. Methane estimation increased with the use of concentrate ration or complete ration containing 15% soybean pod, but it decreased when the level of soybean pod was increased to 30%. It can be concluded that soybean pod has a potential to be used as a fiber source in beef cattle ration to substitute native grass.
Evaluasi Fraksi Serat untuk Mengestimasi Relative Feed Value pada Tanaman Sorgum Galur Mutan Teguh Wahyono; Dewi Apri Astuti; Anuraga Jayanegara; I Komang Gede Wiryawan; Irawan Sugoro
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 15, No 2 (2019): Desember 2019
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.65 KB) | DOI: 10.17146/jair.2019.15.2.5281

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Sorgum G5 dan G8 adalah dua sorgum galur mutan yang diproyeksikan sebagai sorgum khusus pakan ternak. Sampai saat ini, belum terdapat cukup informasi terkait profil serat yang terkandung dalam kedua galur mutan tersebut. Profil serat yang diasosiasikan dalam fraksi neutral detergent fiber (NDF) dan acid detergent fiber (ADF) akan mempengaruhi kecernaan tanaman sorgum. Tujuan dari penelitian ini adalah untuk: 1) mengevaluasi profil fraksi serat dan mengestimasi relative feed value (RFV) pada tanaman sorgum galur mutan, 2) mengevaluasi profil serat pada fase generatif yang berbeda, dan 3) mengetahui profil serat sorgum secara kualitatif menggunakan fourier transform mid-infrared (FTIR). Rancangan yang digunakan adalah rancangan acak kelompok pola faktorial (4x3x3). Faktor pertama adalah varietas/galur mutan tanaman (varietas Numbu, Pahat, galur mutan G5 dan G8). Faktor kedua adalah waktu pemanenan (flowering, soft dough dan hard dough). Faktor ketiga adalah Bagian tanaman (batang, daun dan malai sorgum).  Parameter yang diamati adalah profil serat kuantitatif (NDF dan ADF), RFV dan profil serat secara kualitatif menggunakan FTIR. Hasil penelitian menunjukkan bahwa terdapat pengaruh perbedaan varietas/galur mutan, fase generatif dan bagian tanaman terhadap profil serat tanaman sorgum. Terdapat interaksi antara perbedaan varietas/galur mutan dengan fase generatif tanaman. Sorgum G5 fase hard dough menghasilkan kandungan NDF batang yang lebih rendah dibandingkan Numbu (54,23 % vs 60,41 %) (P<0,01). Sorgum G8 fase hard dough menghasilkan kandungan ADF daun yang lebih rendah dari Numbu (30,07 % vs 32,63 %) (P<0,01). Nilai RFV tertinggi pada bagian batang, daun dan malai berturut-turut dihasilkan oleh sorgum G5, G8 dan Pahat. Hasil pengukuran kualitatif menggunakan FTIR berasosiasi dengan pengukuran NDF dan ADF secara konvensional. Kesimpulan dari penelitian ini adalah: 1) galur mutan G5 dan G8 menghasilkan fraksi sera yang lebih mudah dicerna dibandingkan varietas Numbu. Hal tersebut ditunjukkan oleh nilai RFV yang tinggi pada bagian batang dan daun; 2) Fase hard dough adalah umur panen yang terbaik; dan 3) FTIR dapat digunakan untuk menggambarkan profil serat sorgum secara kualitatif.
Effect of enrichment of organic selenium, inorganic selenium and vitamin E in quail ration on the performances and potency of quail egg as a source of antioxidant Syahrir Akil; Wiranda Gentini Piliang; C. Hanny Wijaya; Desianto Budi Utomo; I Komang Gede Wiryawan
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.156 KB) | DOI: 10.14334/jitv.v14i1.357

Abstract

The change of life style influences human health and it contributes to many human diseases outbreak, therefore nutrition of antioxidant is required. This study was aimed to get an optimum level of combined organic selenium, inorganic selenium and vitamin E in obtaining the best production and reproduction of quails and the highest antioxidant level in quail eggs. This study was conducted from January to August 2008.  Numbers of observed quails were 720 individuals (360 female and 360 male quails). The treatments were applied when the quails were six weeks old. Nine treatment diets were: To (commercial diet), T1 (0.46 ppm inorganic Se + 43.50 ppm vitamin E), T2 (0.46 ppm inorganic Se + 87.00 ppm vitamin E), T3 (0.92 ppm inorganic Se + 43.50 ppm vitamin E), T4 (0.92 ppm inorganic Se +87.00 ppm vitamin E), T5 (0.46 ppm organic Se + 43.50 ppm vitamin E), T6 (0.46 ppm organic Se + 87.00 ppm vitamin E), T7 (0.92 ppm organic Se + 43.50 vitamin E) and T8 (0.92 ppm organic Se 0.92 + 87.00 ppm vitamin E). The design of the experimental applied was a factorial – nested design. Any significant differences among the treatment diets were analysed using Duncan's test. The result of this study indicated that treatment T7 (0.92 ppm organic selenium + 43.50 ppm vitamin E) in general gave the highest content of selenium in meat, in egg albumin, egg yolk, vitamin E in egg yolk, glutathione peroxidase (GSH-Px), activity of antioxidant, hatchability and low mortality. Key Words: Quail, Selenium, Vitamin E, Antioxidant
Gas production and rumen fermentation characteristics of buffalo diets containing by-product from some sorghum varieties Irawan Sugoro; Komang G. Wiryawan; Dewi Apri Astuti; Teguh Wahyono
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.378 KB) | DOI: 10.14334/jitv.v20i4.1241

Abstract

Sorghum is one of potencial fibre sources as buffalo feed. Quality of sorghum could be increased by irradiation mutation breeding. Samurai 1 and Samurai 2 were products of the irradiation mutation breeding of Pahat. This study was conducted to compare buffalo diets containing Samurai 2 sorghum straw and Samurai 1 bagasse sorghum compared with Pahat sorghum straw using in vitro study. Completely randomized design with 6 treatments and 3 replications was applied in this experiment. The treatment diets were P1 (50% Pahat sorghum straw + 50% concentrate), P2 (50% Pahat sorghum straw silage + 50% concentrate), P3 (50% Samurai 2 sorghum straw + 50% concentrate), P4 (50% Samurai 2 sorghum straw silage + 50% concentrate), P5 (50% Samurai 1 sorghum bagasse + 50% concentrate) and P6 (50% Samurai 1 sorghum bagasse silage + 50% concentrate). The 200 mg DM samples of diets were incubated in 30 ml rumen-buffer fluid for 48 hours. Variables measured were total gas production, CH4 production and rumen fermentation characteristics. Results showed that P2 and P4 produce the highest of gas production (P<0.05) with 60.99 and 60.86 ml/200 mg dry matter respectively. Treatments of P1, P2 and P4 produced the lowest CH4 concentration (P<0.05) with 10.57, 10.90 and 9.82% of total gas, respectively. The P4 produced the highest total volatile fatty acids (VFA), dry matter degradability and organic matter degradability with 109.83 mM, 62.93% and 59.97% respectively, meanwhile ammonia (NH3) concentration was not significantly different. The conclusion showed that straw silage of Samurai 2 was comparable to the Pahat sorghum straw and Samurai 1 bagasse sorghum as buffalo diet. Key Words: Buffalo, CH4 Concentration, Rumen Fermentation Characteristics, Sorghum
Synergistic Effect of Biofat and Biochar of Cashew Nutshell on Mitigate Methane in the Rumen Andi Saenab; Komang G Wiryawan; Y Retnani; Elizabeth Wina
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 3 (2020): SEPTEMBER 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i3.2475

Abstract

The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 treatments and 4 replications. A series of biofat (BF) and biochar (BC) combination were added each to substrate as followed BFBC1 = 0: 100%; BFBC2 = 25:75%; BFBC3 = 50:50%; BFBC4 = 75:25%; BFBC5 = 100: 0%. While, a series of biofat (BF) and biosmoke (BS) combination as followed BFBS1 = 0: 100%; BFBS2 = 25:75%; BFBS3 = 50:50%; BFBS4 = 75:25%; BFBS5 = 100: 0%. Both series used a control treatment which contained only substrate. The in vitro experiment was repeated 4 times and each treatment was done in duplicates. The measured variables were: total gas and CH4 productions, dry matter, organic matter, NDF degradability, NH3 and partial VFA concentrations. The results showed that the combination of biofat and biochar levels resulted in a significant decrease (P
Co-Authors Achmad Dinoto Adawiah, Adilah Agus Setiyono Alwi, Windawati Aminuddin Parakkasi Aminuddin Parakkasi Andi Saenab Anhar Faisal Fanani Anja Meryandini Anuraga Jayanegara Ari Wibowo Arif Saefudin Asep Sudarman Asnath M Fuah Asnath Maria Fuah, Asnath Maria Barkah, Nisa Nurmilati C Hanny Wijaya Christina Lini DADIK PANTAYA Desianto Budi Utomo DEWI APRI ASTUTI Didid Diapari, Didid Djumali Mangunwijaja Djumali Mangunwijaya Dudi Firmansyah Dwi Kusuma Purnamasari, Dwi Kusuma Dwi Setyaningsih Dwi Sisriyenni Dwierra Evvyernie Eddy Gurnadi Elizabeth Wina Elizabeth Wina Endang Sulistyowati Endang Sulistyowati Erika B. Laconi Erwan Erwan Fajrih, Nurul Fathin Hamida GINA LESMANA MADUNINGSIH Hairani, Atikah Hanny Hafiar I Nengah Sujaya I Nyoman Suarsana I Wayan Suardana I wayan Teguh Wibawan Iman Rusmana Irawan Sugoro Irawan Sugoro Jakaria Jakaria Jakaria Jakaria Jola J.M.R. Londok Kiston Simanihuruk Kokom Komalasari Lilis Khotijah MADE ASTAWAN Mareistia Fassah, Dilla Maritje Aleonor Hilakore Matutu, Ardi Mawar Mawar Muhammad Daud Nanda Nadhifa Nuraini Negara, Windu Rarah Ratih Adjie Maheswari Rita Mutia Rohmatul Anwar Rudi Priyanto Rudy Priyanto Rusli, Ridho Kurniawan S. Syahrir Salundik Salundik Sigit Mujiharjo Simon P Ginting Sinta Agustina Siti Nur Jannah Sitti Wajizah Soewarno Soekarto Sofia Sandi SRI RAHAYU Sri Suharti Sri Sulastri Suhardi Sumiati Sumiati Sumiati Sumiati Sumiati Sumiati Suryahadi (Suryahadi) Suryahadi Suryahadi Suryahadi Suryahadi Syahriani Syahrir Syahrir Akil Syamsuhaidi Syamsuhaidi TATI NURHAYATI Teguh Wahyono Toto Toharmat Tutik Wresdiyati - Vishara Sekar Fadhilah W. Ramdania Wasmen Manalu Winugroho Winugroho Wiranda G Piliang Wiranda Gentini Piliang Y Retnani Yuli Retnani Yurleni Yurleni Yuspihana Fitrial