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PENGARUH ANGKAK HASIL FERMENTASI BERAS OLEH Monascus purpureus JMBa TERHADAP AKTIVITAS ANTIOKSIDAN DAN GLUTATHION PEROKSIDASE (GPx) SERTA HISTOPATOLOGI HATI TIKUS GALUR SPRAGUE DAWLEY [The effect of Angkak from rice fermented by Monascus purpureus JMBa on antioxidant and glutathion peroxidase (GPx) activity and liver histopatology of Sprague Dawley Rats] Kasim, Ernawati; Triana, Evi; Yulinery, Titin; Nurhidayat, Novik
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.487

Free radicals released from metabolism processes in the body were reactive and toxic to cells and tissues because it cause protein, cell membrane and nucleic acid damages which lead to cancer. It could be overcame if antioxidant system in the body is in good function.Nevertheles antioxidant system could be destructed by variety of conditions. Modern lifestyle that frequently consumed of high saturated fat, additives, and low fiber content in foods were potential risk for cancer and hypercholesterolemia. To overcome that risks with specific drugs were high cost. Therefore this research was conducted to take advantages of natural sources which potential for antioxidant and antihypercholesterolemia activities that could be fast, easy and inexpensive processing. One of the natural sources that meet the criteria was angkak resulted from rice fermented by Monascus purpureus. The result revealed that angkak contained lovastatin showed antioxidant and antihypercholesterolemia activities and increased glutathion peroxidase activity of optimal dose 5 g/day. The histopathologi observation of ratÃ¢â¬â¢s liver showed that administered of angkak on rats feed high level of cholesterol inhibited accumulation of fat in ratÃ¢â¬â¢s liver.

IDENTIFIKASI GEN SELENOMETIL TRANSFERASE (smt) PADA ISOLAT Geobacillus sp. 20K YANG RESISTEN TERHADAP SELENIUM Triana, Evi; Nurhidayat, Novik; Yulinery, Titin; Kasim, Ernawati; Dewi, Ratih M
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The trace element Selenium is toxic at high concentration.Most of organisms living in selenium rich environment are selenium resistant.One of the resistance mechanisms is methylation,in which selenium is methylated and transformed to non-toxic selenium compound.The methylation is catalyzed by seleno methyltransferase (SMT) coded by smt gene. The gene are expressed by selenium tolerant plants. However, there was no available report yet on such specific gene in the bacterial genome. This study was carried out to determine smt homologous gene on selected selenium accumulator bacteria, Geobacillus sp. 20k, The smt gene of was determined by amplifying target DNA and analyses its sequences through homology search (BLAST). The result showed that the DNA and its protein part of thermophilic enzyme involved selenium metabolisms.

Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Triana, Evi; Nurhidayat, Novik; Rahayu, Sri Hartin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.

Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Triana, Evi; Nurhidayat, Novik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.

IDENTIFIKASI GEN TERMOASIDOFILIK ALKOHOL 1 DEHIDROGENASE PADA BACILLUS SP-PJV MS, Yulia Atika; Artika, I Made; Nurhidayat, Novik
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1872

One of the constrains in the conversion process of biomass to bioethanol is the unoptimum technology for the conversion process.One of the causes is the difference between optimum temperature for enzymatic hydrolytic saccharification and for fermentation.Enzymatic hydrolysis requires high temperature and acidic pH, while fermentation operates at mild condition.Hence, thermophilic fermentative microbes are needed so that simultaneous saccharification and fermentation processes can be carried out.Alcoholdehydrogenase(ADH), an enzyme functions in the final step of fermentation, catalyzes reduction of acetaldehydeto ethanol so that it being one of indicator fermentative microbial.Protium javanicum, a typical fruit from Lombok grow at high temperature and has sour taste so that it predicted has a thermo acidophilic ADH.This study was aimed toidentify gene encoding ADHin Protiumjavanicum(Pjv) microbial isolates.ADH gene identification was carried out by DNA amplification using spesific adh primers inqPCR machine. Results showed that all isolates tested had adh gene and that of Bacillus sp-Pjv was the most efficiently amplified. Results of fermentationtest also showed that the Bacillus sp-Pjv isolate was a better ethanol-producer than the others.

Highly Sensitive Phenol Biosensor Utilizing Selected Bacillus Biofilm Through an Electrochemical Method Ariyanti, Dita; Iswantini, Dyah; Sugita, Purwantiningsih; Nurhidayat, Novik; Effendi, Hefni; Ghozali, Ali Aulia; Kurniawan, Yehezkiel Steven
Makara Journal of Science Vol. 24, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus isolate code 100 was selected because it produced an adequate amount of tyrosinase enzyme and a high potentiostat current. The response surface methodology was also used to optimize the phenol sensing condition through an electrochemical method. Results showed that the optimum condition was achieved after 6 days on a phosphate buffer solution (pH of 8), with an optical density of 0.33. Furthermore, the limits of detection and quantification were 3.0 and 13 ng/L, respectively. The measurements of precision yielded a relative standard deviation of < 5%, which is remarkable. Although the biosensor material was used for 35 days, the current throughout was still maintained at 90%, indicating that the evaluated biosensor material has the potential to be used for phenol monitoring on environmental samples in the near future.

Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol 29, No 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.

Superoxide Anion Radicals Biosensor Based on Protein Extract from Deinococcus Radiodurans Immobilization by Glutaraldehyde Cross-Linked Afifi, Muhammad Ridho; Iswantini, Dyah; Nurhidayat, Novik; Saprudin, Deden
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.153-160

A common enzyme for superoxide anion radicals biosensors is superoxide dismutase (SOD). Deinococcus radiodurans protein extract can replace the use of pure SOD enzymes for superoxide anion radicals biosensors. The purpose of this research is to determine the analytical performance of the superoxide anion radicals biosensor with protein extract of Deinococcus radiodurans immobilized on carboxymethylcellulose-gelatin-zeolit (CMC-G-Z) that is crosslinked by glutaraldehyde. The response surface method showed that the optimum condition for the biosensor was pH 7, protein extract concentration of 1075 µg/ml, zeolite concentration of 5 mg/ml, and glutaraldehyde concentration of 0.0042 M. Stability of the biosensor retained 67% of its sensitivity after use for 24 hours. The biosensor exhibits good analytical performance with a linear range from 0.1 – 0.8 mM, a detection limit of 77.84 µM, and a limit of quantification of 259.5 µM with a correlation coefficient of 0.9905.

Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol. 29 No. 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.

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