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Figure 4. Effect of incubation time on KAS II activity. The purified enzyme was incubated under standard conditions for various periods. At the time specified, the reaction was stopped by adding 40% perchloric acid. Means + S.D. (n=3).

Figure 4 Effect of incubation time on KAS II activity. The purified enzyme was incubated under standard conditions for various periods. At the time specified, the reaction was stopped by adding 40% perchloric acid. Means + S.D. (n=3).

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Note: Each individual sample was measured in triplicate. *Means + s.d. (n=5). bMeans +s.d. (n = 4). ‘Significantly different (p<0.001) between Elaeis guineensis, Elaeis oleifera and hybrids. *Iodine value was determined according to PORIM Test Method (1995). TABLE 1a. KAS II ACTIVITY, IODINE VALUE* AND FATTY ACID COMPOSITIONS OF SELECTED THREE OIL PALM POPULATIONS (Elaeis guineensis', hybrids? and Elaeis oleifera*)
Note: Each individual sample was measured in triplicate. *Means + s.d. (n=5). bMeans +s.d. (n = 4). ‘Significantly different (p<0.001) between Elaeis guineensis, Elaeis oleifera and hybrids. *Iodine value was determined according to PORIM Test Method (1995). TABLE 1a. KAS II ACTIVITY, IODINE VALUE* AND FATTY ACID COMPOSITIONS OF SELECTED THREE OIL PALM POPULATIONS (Elaeis guineensis', hybrids? and Elaeis oleifera*)
Figure1. KAS Il activity at various stages of fruit development. Oil palm inflorescences were tagged and fruits were freshly harvested at various stages. Enzyme activities were assayed as described under Materials and Methods. Means + S.D. (n = 3).
Figure1. KAS Il activity at various stages of fruit development. Oil palm inflorescences were tagged and fruits were freshly harvested at various stages. Enzyme activities were assayed as described under Materials and Methods. Means + S.D. (n = 3).
Note: Regression analysis was performed using data from Table 1a. TABLE 1b. REGRESSION ANALYSIS OF RELATIONSHIP BETWEEN KAS II ACTIVITY, PALM OIL FATTY ACIL COMPOSITION AND IODINE VALUE (IV)
Note: Regression analysis was performed using data from Table 1a. TABLE 1b. REGRESSION ANALYSIS OF RELATIONSHIP BETWEEN KAS II ACTIVITY, PALM OIL FATTY ACIL COMPOSITION AND IODINE VALUE (IV)
Figure 2. Purification of oil palm KAS IL. Elution profile of KAS II on: (a) HR-DEAE column, (b) hydroxylapatite column and (c) ACP-Sepharose column as described under Materials and Methods. Note: “Recovery from pooled peak fractions.
Figure 2. Purification of oil palm KAS IL. Elution profile of KAS II on: (a) HR-DEAE column, (b) hydroxylapatite column and (c) ACP-Sepharose column as described under Materials and Methods. Note: “Recovery from pooled peak fractions.
3IOCHEMICAL STUDIES AND PURIFICATION OF OIL PALM (Elaeis guineensis Jacq.) B-KETOACYL-ACYL-CARRIER-PROTEIN (ACP) SYNTHASE (KAS) II
3IOCHEMICAL STUDIES AND PURIFICATION OF OIL PALM (Elaeis guineensis Jacq.) B-KETOACYL-ACYL-CARRIER-PROTEIN (ACP) SYNTHASE (KAS) II
Figure 3. Isoelectric gel focusing on purified KAS IIL. Purified oil palm KAS II was loaded into SDS-polyacrylamide gel and subjected to isoelectric focusing as described under Materials and Methods. The gel was analysed on a polyacrylamide gel containing Pharmalyte 2.5 — 5.0.
Figure 3. Isoelectric gel focusing on purified KAS IIL. Purified oil palm KAS II was loaded into SDS-polyacrylamide gel and subjected to isoelectric focusing as described under Materials and Methods. The gel was analysed on a polyacrylamide gel containing Pharmalyte 2.5 — 5.0.
Figure 5. Effect of incubation pH on KAS II activity. The purified enzyme was incubated in various buffers; 50 mM acetate pH 4.0-5.5, potassium phosphate pH 6.0-8.5, and glycine pH 9.0. Means + S.D. (n=3).
Figure 5. Effect of incubation pH on KAS II activity. The purified enzyme was incubated in various buffers; 50 mM acetate pH 4.0-5.5, potassium phosphate pH 6.0-8.5, and glycine pH 9.0. Means + S.D. (n=3).
Figure 6. Effect of divalent cations on KAS II activity. The assay was carried out in the presence of divalent cations at 1 mM and 10 mM concentrations. Means +S.D. (n=3).
Figure 6. Effect of divalent cations on KAS II activity. The assay was carried out in the presence of divalent cations at 1 mM and 10 mM concentrations. Means +S.D. (n=3).
Figure 7. Effect of EDTA on KAS II activity. Panel (a) shows KAS II activity in 1 mM EDTA and panel (b) the KAS II activity regained by the addition of metal cations, Mg** and Mn**. Means + S.D. (n=3).
Figure 7. Effect of EDTA on KAS II activity. Panel (a) shows KAS II activity in 1 mM EDTA and panel (b) the KAS II activity regained by the addition of metal cations, Mg** and Mn**. Means + S.D. (n=3).
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