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Fig. 4 : Effect of elicitors on cell growth and proliferation a. Methyljasmonate (MJ); b. Yeast extract (YE); c. Pectin; d. Chitin

Figure 4 : Effect of elicitors on cell growth and proliferation a. Methyljasmonate (MJ); b. Yeast extract (YE); c. Pectin; d. Chitin

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Fig. 1 Structure of gymnemagenin In yeast extract treated cultures, at 0.5 g 1”! concentra- tion for 20 days of treatment elicited the highest gymnemic acid (5.32 mg/500 mg DW) production. While at other concentrations also elicited higher production of gymnemic acid, with 1.5 g 1 concentration for 15 days (2.24 mg/ 500 mg DW), 2 g' concentration for 20 days (1.95 mg/ 500 mg DW) of treatment and 1 g™! concentration for
Fig. 1 Structure of gymnemagenin In yeast extract treated cultures, at 0.5 g 1”! concentra- tion for 20 days of treatment elicited the highest gymnemic acid (5.32 mg/500 mg DW) production. While at other concentrations also elicited higher production of gymnemic acid, with 1.5 g 1 concentration for 15 days (2.24 mg/ 500 mg DW), 2 g' concentration for 20 days (1.95 mg/ 500 mg DW) of treatment and 1 g™! concentration for
cultures there was a gradual accumulation of gymnemic acid content till the 15th day (46. 7 + 0.31% DW), while in 0.4% treated cultures there was an initial increase in gymnemic acid content (49.07 + 0.17% DW) till 5th day, that decreased thereafter. A grad ual increase in gymnemic acid content was observed in 0.6% pectin treated cultures, that reached highest value of 64.19 + 0.19% DW at the end of 20 days of culture period. While, in cultures treated 10 days (1.67 mg/500 mg DW) compared to respective controls. The highest yield of gymnemagenin elicited with 0.5 g! of yeast extract, 20 days is sixfolds higher than the respective control (Fig. 3b). Yeast extract also affected cell growth as indicated by the decrease in dry weight (Fig. 4b). srowth as indicated by the decrease in dry weight (Fig. 4b). In Pectin treated cultures, at 0.6% pectin concentration for 20 days of treatment elicited highest gymnemic acid (64.19 + 0.19%DW) production. In 0.2% pectin treated
cultures there was a gradual accumulation of gymnemic acid content till the 15th day (46. 7 + 0.31% DW), while in 0.4% treated cultures there was an initial increase in gymnemic acid content (49.07 + 0.17% DW) till 5th day, that decreased thereafter. A grad ual increase in gymnemic acid content was observed in 0.6% pectin treated cultures, that reached highest value of 64.19 + 0.19% DW at the end of 20 days of culture period. While, in cultures treated 10 days (1.67 mg/500 mg DW) compared to respective controls. The highest yield of gymnemagenin elicited with 0.5 g! of yeast extract, 20 days is sixfolds higher than the respective control (Fig. 3b). Yeast extract also affected cell growth as indicated by the decrease in dry weight (Fig. 4b). srowth as indicated by the decrease in dry weight (Fig. 4b). In Pectin treated cultures, at 0.6% pectin concentration for 20 days of treatment elicited highest gymnemic acid (64.19 + 0.19%DW) production. In 0.2% pectin treated
Fig. 3 Effect of elicitors on gymnemic acid production a. Methylj- asmonate (MJ); b. Yeast extract (YE); c. Pectin; d. Chitin
Fig. 3 Effect of elicitors on gymnemic acid production a. Methylj- asmonate (MJ); b. Yeast extract (YE); c. Pectin; d. Chitin
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