Body odors change with health status and the odors of sick animals can induce avoidance behaviors in healthy conspecifics. Exposure to sickness odors might also alter the physiology of healthy conspecifics and modify the odors they...
moreBody odors change with health status and the odors of sick animals can induce avoidance behaviors in healthy conspecifics. Exposure to sickness odors might also alter the physiology of healthy conspecifics and modify the odors they produce. We hypothesized that exposure to odors of sick (but non-infectious) animals would alter the odors of healthy cagemates. To induce sickness, we injected mice with a bacterial endotoxin, lipopolysaccharide. We used behavioral odor discrimination assays and analytical chemistry techniques followed by predictive classification modeling to ask about differences in volatile odorants produced by two types of healthy mice: those cohoused with healthy conspecifics and those cohoused with sick conspecifics. Mice trained in Y-maze behavioral assays to discriminate between the odors of healthy versus sick mice also discriminated between the odors of healthy mice cohoused with sick conspecifics and odors of healthy mice cohoused with healthy conspecifics. Chemical analyses paired with statistical modeling revealed a parallel phenomenon. Urine volatiles of healthy mice cohoused with sick partners were more likely to be classified as those of sick rather than healthy mice based on discriminant model predictions. Sickness-related odors could have cascading effects on neuroendocrine or immune responses of healthy conspecifics, and could affect individual behaviors, social dynamics, and pathogen spread. Living in groups can increase access to resources such as food, shelter, and mates 1 , but can also impose direct costs on individuals, such as increased competition, greater conspicuousness to predators, and higher risk of infections transmitted from conspecifics . The ability to identify and respond to indicators of health status is therefore critical in groups. Many animals, including humans, have evolved sophisticated sensory mechanisms to detect the risk of contagion in the social environment . Visual, auditory, somatosensory, and olfactory cues are processed by the central nervous system and can orchestrate physiological and behavioral responses, enabling an organism to optimize survival and reproductive success under potentially adverse circumstances . Chemical cues are key mediators of two-way communication in social animals 6,8,12-14 , and convey fixed characteristics of individuals such as sex, age, and relatedness, as well as dynamic traits, such as physical condition, social dominance status, and health . Body odor may be among the most salient indicators of risk of contagion in an individual's social environment , particularly if visual or other types of pathological signs of infection or illness are absent, attenuated, or socially suppressed 18 . A large body of literature has shown that chronic disease, pathogenic and parasitic infection, inflammation, and vaccination can alter body odors . The consequences of such odor changes can be context-dependent , but experimental studies with rodents and humans have generally shown that healthy individuals limit their investigation of and rate as more aversive the odors of sick or infected, compared to healthy individuals . Several studies have specifically examined behavioral responses of healthy rodents to the odors of conspecifics injected with the non-replicating bacterial endotoxin, lipopolysaccharide (LPS) . Notably, most of these behavioral assessments of responses to the odors of LPS-injected individuals have involved presentation of odor stimuli (e.g., live animals or their urine or bedding material) within 4 hours of injection . This early time point following LPS injection is associated with robust pro-inflammatory immune responses, increased body temperature (fever), and the expression of sickness behaviors such as lethargy, anorexia, and self-isolation . It is unknown whether avoidance or constrained social investigation of sick animals and their odors by healthy conspecifics persists beyond early