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Abstract
Introduction Biogeography Overview
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References

A JOURNEY IN SEARCH OF Tetragonula biroi (Friese 1898) PROPOLIS

Profile image of Abu Hassan JalilAbu Hassan Jalil

2022, AGRIKULTURA: Central Bicol State University of Agriculture Research and Innovation Journal

Abstract

This paper looks into the mapping of Tetragonula biroi in the different islands of the Phlippines exploring into the propolis sources, the derivatives from different sources and exudates, harvesting and processing of propolis, and other research gaps involving the T. biroi species across regions. The dominant vegetation and host trees were scrutinized for the possible exudates utilized and processed by the bees. These are tabulated and charted with images from the Meliponaries, Bee Farms and Eco-Parks visited in many different islands of the Philippines regions. The resultant propolis and cerumen textures and consistency were compared for value-added product derivative considerations. The considerations include possibilities for use in aromatherapy, cosmeceuticals, nutraceuticals and pharmaceuticals. Some recommendations on the chemical processes and procedures for product derivatives are provided herein. Harvesting and post-harvest modus operandi are suggested with references from F.A.O. literature and past experiences.

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References (40)

  1. Appanah, S., & Turnbull, J.W (eds) 1998. A Review of Dipterocarps: Taxonomy, Ecology and Silviculture. Publisher: Center for International Forestry Research, Indonesia. Retrieved from: https://doi.org/10. 17528/cifor/000463.
  2. Baltazar, C. R. 1966. A Catalogue of Philippine Hymenoptera (with a bibliography, 1758-1963). Pacific Insects Monograph 8: 1-488. Retrieved from: http://scinet.science .ph/union/Downloads/Baltazar,%20 Clare%20R.%201966%20Pacific% 20Insects%20Monograph%208 (library)_14.pdf.
  3. Boonthai, S. and Sawatthum, A., 2014, Efficacy of stingless bee as Insect pollinator of rambutan var. Sri Tong, pp. 214-215, In The 5th Phetchaburi Rajabhat University Research for Sustainable Thailand National
  4. Conference, Phetchaburi. Chandra, G. (n.d.). Wallace's Line, Weber's Line and Wallacea. Retrieved from IASZoology.com. http://www.ias zoology.com/wallacea/.
  5. Ciar, R. R., Bonto, L.S., Bayer, Mc H.P., Rabajante, J.F., Lubag, S.P., Fajardo, A.C., Cervancia, C.R. (n.d). Foraging Behavior of Stingless Bees (Tetragonula biroi Friese): Distance, Direction and Height of Preferred Food Source. Retrieved from: https://arxiv.org/ftp/ arxiv/papers/1310/1310.3919.pdf. Cortopassi-Laurino, M., Imperatriz- Fonseca, V.L., Roubik, D.W., Dollin, A., Heard, T., Aguilr, I., Venturier, G. C., Eardley, C., & Nogueira-Neto, P. 2006. Global meliponiculture: challenges and opportunities. Apidologie 37, 275-292. Retrieved from: https://www.apidologie.org/ articles/apido/pdf/2006/02/m6035sp. pdf.
  6. Eltz, T., Bruhl, C.A., Imiyabir, Z., & Linsenmair, K.E. 2003. Nesting and nest trees of stingless bees (Apidae; Meliponini) in the lowland dipterocarp forest in Sabah, Malaysia, with implications for forest management. Forest Ecology and Management 172, 301-313.
  7. Esselstyn, J. A., Oliveros, C.H., Moyle, R. G., Peterson, A.T., McGuire, J.A. & Brown, R.M. 2010. Integrating phylogenetic and taxonomic evidence illuminates complex biogeographic patterns along with Huxley's modification of Wallace's Line. Journal of Biogeography 37 (11), 2054-2066. Retrieved from: https://doi.org/10.1111/j.1365-2699. 2010.02378.x.
  8. Freise, H. 1898. Die Trigona-arten Australiens. Termeszetrajzi Fuzetek 21: 427-431.
  9. Jalil, A. H. 2014. Beescape for Meliponines: Conservation of Indo-Malayan Stingless Bees. Partridge Publishing Singapore. (ISBN: 9781482823622).
  10. Jalil, A. H. 2018. Southern Luzon Meliponiculture Tour. Luzon, The Philippines: Akademu Kelulut
  11. Malaysia Sdn Bhd.. (ISBN: 978-967- 2290-14-8).
  12. Jalil, A. H. 2019. Cohabitation of Meliponines in Ants' or Termites' Arvoreal nests. Alademi Kelulut Malaysia Sdn Bhd. (ISBN: 978-967- 2290-15-5).
  13. Jalil, A. H. 2019. Visayas Meliponiculture Tour. Cebu, The Philippines: Akademi Kelulut Malaysia Sdn Bhd. (ISBN: 978-967-2290-16-2).
  14. Jalil, A. H., & Roubik (ed), D. W. 2019. Filipino Meliponiculture and Beyond inc. MIMAROPA Tours. Mindoro, The Philippines: Akademu Kelulut Malaysia Sdn Bhd. (978-967-2290- 19-3).
  15. Jalil, A., & Roubik(ed), D. 2016. Handbook of Meliponiculture. Akademi Kelulut Malaysia Sdn Bhd. (ISBN: 978-967- 14783-0-1).
  16. Jalil, A., & Roubik(ed), D. 2018. Handbook of Meliponiculture Vol 2. Akademi Kelulut Malaysia Sdn Bhd. (ISBN: 978-967-2290-11-7).
  17. Krell, R. 1996. Value-Added Products from Beekeeping. FAO Agricultural Services Bulletin, 124, Food and Agriculture Organization of the United Nations, Rome, Italy. (ISBN 92-5-103819-8). Retrieved from: h t t p s : / / w w w. a p i s e r v i c e s . b i z / documents/articles-en/value_added _products_from_beekeeping.pdf.
  18. Leonhardt, S. D., Zeilhofer, S. Blüthgen , N. and Schmitt, T. 2010. Stingless Bees Use Terpenes as Olfactory Cues to Find Resin Sources, Chemical Senses, 35 (7) : 603-611. Retrieved from: https://doi.org/10. 1093/chemse/bjq058.
  19. Leonhardt, S.D., Jung, L.M., Schmitt, T. and S. Blüthgen, S. 2010.. Terpenoids tame aggressors: role of chemicals in stingless bee communal nesting. Behavioral Ecolology and Sociobioiology. 64: 1415-1423. Retrieved from: https:// doi.org/10.1007/s00265-010-0956-
  20. Michener, C. D. 1961. Observations on the Nests and Behavior of Trigona in Australia and New Guinea (Hymenoptera, Apidae). American Museum Novitates, No. 2026, 46pp. Retrieved from: http://hdl.handle.net /2246/3473.
  21. Michener, C. D. 2007. The Bees of the World 2nd ed. John Hopkins University Press, Baltimore, 953pp. Retrieved from: https://static1. squarespace.com/static/5a849d4c8 dd041c9c07a8e4c/t/5ad3bc968a92 2d44a4728936/1523825933048/ Michener+2007+The+Bees+of+the +World.pdf.
  22. Mostoles, MDJ and S.M. Baja. 2017. Colony Characterization and Amplicon Sequencing of Stingless Bees (Tetragonula biroi). Proceedings of the Conference, Malaysia. p. 27. Retrieved from: https://cbsua.edu.ph/wp-content/ uploads/2021/09/STINGLESS- BEES-HYMENOPTERA-APIDAE -MELIPONINI-UNDER-GENUS- Tetragonula-IN-THE-PHILIPPINES. pdf. Mostoles, M. D. 2017. Meliponiculture in Philippines - (Meliponiculture Initiatives in Bicol Region). In: A. H. Jalil, Handbook of Meliponiculture. Akademi Kelulut Malaysia Sdn Bhd.
  23. I S B N : 9 7 8 -9 6 7 -1 4 7 8 3 -0 -1 ) . Retrieved from: https://www. academia.edu/35615675/Meliponi culture_in_Philippines.
  24. Moure, J. S. 1961. A preliminary supra- specific classification of the Old World meliponine bees (Hym., Apoidea). Studia Entomologica, 4: 181-.242.
  25. Nogueira-Neto P. 1997. Vida e criaçao de abelhas indigenas sem ferrao, Editora Nogueirapis, Sao Paulo.
  26. O'Connell, D. 2013. An investigation into the cryptic diversity of sunbird species (Nectariniidae) across south-east Sulawesi (Sulawesi Tenggara). B.Sc.(Hons) Zoology, University College Dublin, 80 pp. DOI: 10.13140/2.1.1867.5523.
  27. Poinar, G. O. 1992. Fossil Evidence of Resin Utilization by Insects.
  28. Biotropica, 24 (3): 466-468. Retrieved from: https://doi.org/10.23 07/2388620.
  29. Raes, N., Cannon, C.H., Hijmans, R.J., Piessens, T., Saw L.G., van Welzen, P.C. Ferry Slik, J.W.. 2014. Historical distribution of Sunda land's Dipterocarp rainforests at Quaternary glacial maxima. Proceedings of the National Academy of Sciences, 111 (47): 167
  30. -16795. Retrieved from: https:// doi.org/10.1073/pnas.1403053111.
  31. Rasmussen. 2008. Catalogue of the Indo -Malayan/Australasian stingless bees (Hymenoptera: Apidae: Meliponini). Zootaxa. 1935 (1):80pp. ISBN 978-1-86977-295-6 (paper back). DOI: https://doi.org/10.11646/ zootaxa.1935.1.1.
  32. Roubik, D. 2006. Stingless bee nesting biology. Apidologie 37 (2): 124-143. DOI: 10.1051/apido:2006026.
  33. Sakagami, S. F. 1978. Tetragonula Stingless Bees of the Continental Asia and Sri Lanka (Hymenoptera, Apidae). Journal Faculty Science Hokkaido Univ. Ser. VI, Zool. 21 (2): 165-247. Retrieved from: http://hdl. handle.net/2115/27635.
  34. Sakagami, S. F., Inoue, T., Yamane, S., & salmah, S. 1989. Nests of the Myrmecophilous Stingless Bee, Trigona moorei: How do Bees Initiate Their Nest Within an Arboreal Ant Nest? Biotropica, 21 (3): 265-274.
  35. Schwarz, H. F. 1937. Results of the Oxford University Sarawak (Borneo) expedition: Bornean stingless bees of the genus Trigona. Bulletin American Museum of Natural History. 73: 281-328. Retrived from: http://hdl.handle.net/2246/870.
  36. Schwarz, H. F. 1939. The Indo-Malayan Species of Trigona. Bulletin American Museum of Natural History. 76: 83-141. Retrieved from: http://hdl.handle.net/2246/867.
  37. Starr, C. K.and Sakagami, S. F. 1987. An Extraordinary Concentration of Stingless Bee in the Philippines, with Notes on Nest Architecture (Hymenoptera: Apidae: Trigona spp). Insectes Sociaux. 34 (2): 96- 107. Retrieved from: https://link. springer.com/content/pdf/10.1007/ BF02223828.pdf.
  38. Van Welzen, P.C., Parnell, J.A.N. and Ferry Slik, J.W.. 2011. Wallace's Line and plant distributions: Two or three phytogeographical areas and where to group Java? Biological Journal of the Linnean Society 103 (3): 531-545. https://doi.org/10.1111/ j.1095-8312.2011.01647.
  39. Vit, P., Pedro, S., & Roubik, D. 2018. Pot -Pollen in Stingless Bee Melittology. Springer International Publishing AG. DOI: 10.1007/978-3-319-61839- 5.
  40. Vit, P., Roubik, D. W., & Pedro, S. M. 2013. Pot Honey -A Legacy of Stingless Bees. Springer, DOI -10.1007/978- 1-4614-4960-7.

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