Bee Propolis Trigona spp Potential and uniqueness in Indonesia

Tarım Bilimleri Dergisi, 2021

Honey bees collect the main material of propolis from the buds, leaves, branches, and barks or other botanical sources and mixed that resinous material with beeswax produced from abdominal exocrine glands, mandibular gland secretions, and pollen to produce propolis. The composition of propolis changes depends on multiple factors such as honey bee races, geographical locations, phytogeography, harvesting seasons, extraction methods and solvents. In this study, two different studies were conducted in two different locations in order to reveal the effect of race and season variables on the composition and antioxidant value of propolis. The effect of race factor was studied on three different honey bee races (Apis mellifera caucasica, Apis mellifera syriaca and Apis mellifera carnica) and two different ecotypes (Muğla ecotype and Yığılca ecotype) to investigate the effects of race factor on propolis composition in Central Anatolia by under the controlled conditions. The effect of seasonal change was determined by Yığılca ecotype of A. melifera anatoliaca in the apiary located in Yığılca, Düzce location. Studied samples were harvested by propolis trap between May and October. Total phenolic content, total antioxidant capacity and chemical profiles of propolis samples were determined using HPLC-UV. The obtained results showed that honey bee race and season have an effect on the antioxidant capacity and chemical composition of propolis.

International Journal of Applied Pharmaceutics, 2024

Objective: Propolis is a bee product collected by honeybees from a resinous substance of various plant sources. Its antioxidant activities are different from various geographic origins. This study aimed to analyze bioactive compounds by lC-MS/MS, compare antioxidant activity total phenolic and flavonoid contents in stingless bee propolis samples from several regions in Indonesia. Methods: The propolis samples were taken from stingless bee hives of Tetragonula clypearis from Sumbawa, Tetragonula laeviceps from Magelang, Tetragonula biroi from Bogor, and Geniotrigona thoracica from South Kalimantan). Analysis of bioactive compounds was identified by lC-MS/MS. The quantification of the chemical compound determined its total phenolics and flavonoid (TPC and TFC) contents. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay.

Food Science and Technology International, 2020

Propolis is a highly complex resinous substance collected by honeybees from the fresh plant buds or secretions and mixed with beeswax and utilized for different purposes in the hive and includes more than 300 primary compounds (Guo et al., 2015). The human medicine has been interested in this product, used for several purposes, such as paving the hive surfaces, filling the cracks and fractures, polishing the honeycomb cells, narrowing and widening the hive entrance, and coating and isolating possible disease agents, for thousands of years, and it was used in several areas, including mummification and to treat mouth sores (Fokt et al., 2010; Can et al., 2015).

Heliyon, 2020

Propolis and honey produced by stingless bees are regarded as high economic value products due to their bioactive components, which are significantly influenced by conditions at the cultivation location. This study investigated the effect of cultivation location on the amount and quality of propolis and honey produced by Tetragonula laeviceps cultivated in Modular Tetragonula Hives. Fifteen bee colonies were cultivated for at least three months in coffee plantations at two different locations, namely Cibodas and Cileunyi Wetan, Indonesia. The propolis was harvested from the hives and then evaluated to compare product quality from each location. The average production of propolis in both locations was found to lie in the range of 4.26-4.54 g/colony/month with a flavonoid content of 11.4-14.8 mg/g qE. Meanwhile, the average production of honey in both locations after eight months of cultivation was found to lie in the range of 0.93-1.44 g/colony/month. The vitamin C content of the honey obtained from both locations was 17.2-69.5 mg/100 g with an IC 50 of 1188-1341 mg/L, in terms of its ability to inhibit the free radical 2,2-diphenyl-2-picrylhydrazyl. This study shows that cultivation of stingless bees on a coffee plantation in the studied locations has the potential to provide sustainable production of propolis and honey from T. laeviceps.

Cancer is now one of the leading causes of death in developing countries, killing more people globally each year than any other. By 2020 there are expected to be 15 million new cases of cancer every year globally, 70% of which will be in developing countries. International Status: It is estimated that about 9 million new cancer cases are diagnosed every year and over 4.5 million people die from cancer each year in the world. National Status: The estimated number of new cancers in India per year is about 7 lakhs and over 3.5 lakhs people die of cancer each year. Bangalore tops the list of metros in the country with the highest number of women suffering from cancer and breast cancer continues to be the killer, according to the Population Based Cancer Registry (PBCR). Propolis, a bee hive product rich in natural flavonoids can be used as a drug for cancer. A lot of research is being carried out worldwide directed toward finding natural antioxidants and antitumor activity in propolis. Methanolic extract of Indian Propolis was screened for flavonoid content responsible for antitumor activity. Materials and Methods-Aluminum chloride method for total flavonoid content and GCMS for Quantification of flavonoids. Conclusions: Our findings provide evidence that methanolic extract of propolis is a potential source of Lupeol which can be isolated and used for cancer therapy.

Propolis is well known for its valuable biological activities widely used in the treatment of many diseases and beneficial effects on human. It is rich in chemical compounds which depend on the type of plant accessible to the bees. In the present study, the chemical composition of 6 Indian propolis samples were analysed using GCMS where 93 compounds were identified and 14 being new for Indian propolis. These propolis were characterized by the presence of significant amount of carboxylic acid (20.4%), terpenoids (15.0%), steroids (11.5%), hydrocarbons (9.6%), sugars (6.4%), alkaloids (6.4%), flavonoids (4.3%), phenols (3.2%), ketones (2.1%), amino acid (2.1%), vitamins (2.1%) and other compounds (15.0%). In addition, compounds such as carbohydrates, amino acids, lipids, vitamin C, phenols, flavonoids, alkaloids and terpenoids were quantified. According to chemical composition of propolis, the Indian propolis is a promising source of new bioactive compounds, which need identification of compounds responsible for the biological activity. Keywords- Propolis, chemical constituents, GCMS, polyphenols, hydrocarbons, steroids.

2000

International Journal of Pharmacy and Pharmaceutical Sciences, 2021

Objective: The aim of this study is to map out the distribution and composition of the main active components found in stingless bee propolis from various regions in Indonesia. Methods: The stingless bee propolis used was obtained from ten different provinces in Indonesia and the active components analysis using Gas Chromatography-Mass Spectrometer (GC-MS) pyrolyzer. Results: This study found 85 main types of active components with concentrations of ≥ 1%. The most frequently found active component was alpha-d-glucopyranoside, which had an average concentration of 28.20%. Conclusion: There were differences between the main active components found in 14 samples of stingless bee propolis obtained from 10 provinces in Indonesia, which was due to the variety of bee species and plant origin.

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

In this study, it was investigated whether different bee breeds prefer different plant sources to collect propolis. For this purpose four different honey bee race (Apis mellifera caucasica, A. m. carnica, A. m. syriaca and A. m. anatoliaca) naturally have been in Turkey were placed in the same isolated apiary; and Propolis was harvested from these races. chemical contents of alcoholic extractions of the harvested propolis were were analyzed by liquid chromatography-tandem mass spectrometry (LCMS / MS). In addition that, the pollen content of the same propolis samples were determined with a microscope. According to the LCMS / MS results the propolis samples collected by different honey bee race differed significantly in terms of quercetin and ferulic acid. Data obtained from polen analyses revealed that Fabaceae and Apiaceae (PD >45%) families were mostly detected in propolis samples obtained from different races. Although the polen from the Campanulaceae family was dedected only ...

International Journal of Pharmacy and Pharmaceutical Sciences, 2021

The aim of this study is to map out the distribution and composition of the main active components found in stingless bee propolis from various regions in Indonesia. Methods: The stingless bee propolis used was obtained from ten different provinces in Indonesia and the active components analysis using Gas Chromatography-Mass Spectrometer (GC-MS) pyrolyzer. Results: This study found 85 main types of active components with concentrations of ≥ 1%. The most frequently found active component w as alpha-d-glucopyranoside, which had an average concentration of 28.20%. Conclusion: There were differences between the main active components found in 14 samples of stingless bee propolis obtained from 10 provinces in Indonesia, which was due to the variety of bee species and plant origin.