Papers by Ajibola D . Adelakun
EWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed ... more EWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed across cell lines and involved in multiple parts of RNA processing, such as transcription, splicing, and mRNA transport. EWSR1 has also been implicated in cellular mechanisms to control formation of Rloops, a three-stranded nucleic acid structure consisting of a DNA:RNA hybrid and a displaced singlestranded DNA strand. Unscheduled R-loops result in genomic and transcription stress. Loss of function of EWSR1 functions commonly found in Ewing Sarcoma correlates with high abundance of R-loops. In this study, we investigated the mechanism for EWSR1 to recognize an R-loop structure specifically. Using electrophoretic mobility shift assays (EMSA), we detected the high affinity binding of EWSR1 to substrates representing components found in R-loops. EWSR1 specificity could be isolated to the DNA fork region, which transitions between double-and single-stranded DNA. Our data suggests that the Zinc-finger domain (ZnF) with flanking arginine and glycine rich (RGG) domains provide high affinity binding, while the RNA recognition motif (RRM) with its RGG domains offer improved specificity. This model offers a rational for EWSR1 specificity to encompass a wide range in contexts due to the DNA forks always found with R-loops. .
EWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed ... more EWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed across cell lines and involved in multiple parts of RNA processing, such as transcription, splicing, and mRNA transport. EWSR1 has also been implicated in cellular mechanisms to control formation of R-loops, a three-stranded nucleic acid structure consisting of a DNA:RNA hybrid and a displaced single-stranded DNA strand. Unscheduled R-loops result in genomic and transcription stress. Loss of function of EWSR1 functions commonly found in Ewing Sarcoma correlates with high abundance of R-loops. In this study, we investigated the mechanism for EWSR1 to recognize an R-loop structure specifically. Using electrophoretic mobility shift assays (EMSA), we detected the high affinity binding of EWSR1 to substrates representing components found in R-loops. EWSR1 specificity could be isolated to the DNA fork region, which transitions between double-and single-stranded DNA. Our data suggests that the Zinc-finger domain (ZnF) with flanking arginine and glycine rich (RGG) domains provide high affinity binding, while the RNA recognition motif (RRM) with its RGG domains offer improved specificity. This model offers a rational for EWSR1 specificity to encompass a wide range in contexts due to the DNA forks always found with R-loops.
Nigerian Journal of Natural Products and Medicine, 2015
The essence of standardization of medicinal plants is to produce herbal medicines with acceptable... more The essence of standardization of medicinal plants is to produce herbal medicines with acceptable safety, efficacy, quality and reproducibility. Allium cepa var. cepa L. (Amaryllidaceae) bulbs purchased from Sasha Market, Ibadan, Nigeria during the rainy season was subjected to organoleptic, morphological and physico-chemical experiments. The total ash gave 5.40 ± 0.001%, acid-insoluble ash (0.62 ± 0.0001%), water-soluble extractive (2.30 ± 0.004%), alcohol-soluble extractive (1.14 ± 0.005%) and moisture content (91.1 ± 0.009%). Microscopical evaluation of the bulb showed distinct cell-walls devoid of nucleus while the inner epidermis possessed well delineated cell-walls, transparent cytoplasm containing nucleus but without stomata. Histochemical experiments showed lignified, suberized and cellulosic cell-walls, starch grains, oils, resins, tannins and anthraquinones. The study has provided some data for the pharmacopoeial monograph on Allium cepa var. cepa bulb as a possible antidiabetic agent for inclusion into the 2 nd editions of the Nigerian Herbal Pharmacopoeial (NHP) and West African Herbal Pharmacopoeial (WAHP). A B C D E Plate 1: Upper epidermal layer of the bulb scale of Allium cepa var. cepa (unstained) Plate 2: Inner epidermal layer of the bulb scale of Allium cepa var. cepa (stained) Key: A = cell-wall, B = cytoplasm, C = nucleus Plate 3: Transverse Section of Allium cepa var. cepa bulb showing 3 scales Key: D = cells contained in a scale, E = spaces between scales Plate 4: Longitudinal Section showing the stem tissues of Allium cepa var. cepa bulb
Book Reviews by Ajibola D . Adelakun
Journal of Phytomedicine and Therapeutics, 2024
Natural dyes are becoming increasingly common for practical finishes that encourage an eco-friend... more Natural dyes are becoming increasingly common for practical finishes that encourage an eco-friendly lifestyle. Natural dyes are renewable materials safe for the environment and do not cause toxicity, cancer, or allergic reactions. Transdermal Drug Delivery offers a pain-free method for delivering medication systemically by administering a drug formulation to intact, healthy skin. Herbal clothing dyed with these herbs has anti-inflammatory, antibacterial, and antioxidant qualities that help the skin and treat dermatitis. Herbal textiles/garments have immense potential in the worldwide textile industry and may one day become a significant textile product. Using herbs and textiles to achieve health while being ecologically conscious is a fantastic way to live a healthy lifestyle. Herbal clothing is becoming increasingly fashionable all around the world. Herbal-treated textiles are one of the most effective approaches to strengthen and extend the worldwide textile market share of the batik business. Herbal clothing is also non-toxic, non-carcinogenic, biodegradable, and eco-friendly, making it an appealing option for modern, well-informed shoppers seeking therapy. Furthermore, developing bio-colorants to meet rising demand will compel entrepreneurs to enter this sector in search of bigger profits, resulting in more employment creation. However, there is a need for standardization of dosages of extract to be used in dying clothes meant for the treatment of specific disease conditions.
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Papers by Ajibola D . Adelakun
Book Reviews by Ajibola D . Adelakun