Papers by Serenella Nardi
Rhizosphere: a communication between plant and soil
Influence of tillage systems on biological properties, organic matter and total N, on soil profile under continuous maize production
Use of organic amendments in ornamental plant nursery production: biological and agronomical evaluation of cultural substrates
Effects of zinc supraoptimal concentrations in wheat (Triticum aestivum L.) seedlings
Available P determination by chemical and P-sink methods in alkaline and calcareous soil samples from Sardinia
Different response to UV-B radiation of nitrate reductase from two maize hybrids
Influence of two different soil management methods on erosive processes and productivity of a young olive-grove in mediterranean environment
The results of a research, which started in april 1997 and is still in progress, carried out in a... more The results of a research, which started in april 1997 and is still in progress, carried out in a ten year old experimental olive-grove located in a hilly area of the Calabria (Italy) are reported. The aim of the present study was to evaluate the influence of two different soil management methods, namely permanent cover crop and ploughing, on runoff, soil and some nutrient losses, such as K, Na, nitrates , phosphates, available P and organic matter. In addition, the behaviour of the main vegetative and productive characteristics of the olive trees (annual growth rate, flowering, fruit set, fruit drop and yield) is reported. The obtained results were analysed by a one-way variance analysis. In order to evaluate the response of the two management systems assayed on the erosive processes trend for every runoff event, a discriminant analysis was also applied. The results reported showed an highly significant improvement of all erosive parameters when permanent cover crop management system was used. Moreover, the data coming from the discriminant analysis, showing a very high percentage (92.7 %) of cases correctly classified by the model, underlined the different behaviour of the two soil management methods also at the single events level.
Humus forestali: manuale di ecologia per il riconoscimento e l’interpretazione: applicazione alle faggete
Indicators of biodiversity, microbial biomass and activity through the profile of an organic soil
Porosity in wet-sieved aggregates and its effects on SOC protection
15th IHSS MEETING "Humic Substances and the Maintenance of Ecosystem Services., 2010
since the organic matter is a binding agent promoting the aggregation, but it is also protected b... more since the organic matter is a binding agent promoting the aggregation, but it is also protected by porosity. The soil pore distribution controls the spatial inaccessibility to microorganisms and the low O2 diffusion, that are very important factors in SOC protection. The smaller pores internal to microaggregates (53\u2013250 μm) are more likely to exclude biota and their enzymes than those of macroaggregates. The aim of this study was to investigate the chemical and physical mechanisms of SOC protection in a long-term experiment established in the early 1960s in North-eastern Ital
Effects of humic fractions on morphological changes related to invertase and peroxidase activities in wheat seedling roots
Chemical and Biological Technologies in Agriculture, Aug 11, 2023
Background The threatening impact of conventional agriculture (CA) on soils could be due to the d... more Background The threatening impact of conventional agriculture (CA) on soils could be due to the detrimental effects on soil microbial communities. Conversely, organic agriculture (OA) is envisaged as potentially enhancing helpful microbial communities and is proposed as environmentally sustainable. The soil microbiome influences soil health and quality, hence, it requires deeper investigation and understanding. In this study, applying 16S metabarcoding and qPCR techniques, we compared the microbial patterns of long-term organically and conventionally managed soils to explore their similarities and differences. Results Total DNA quantification showed an over 20-fold higher amount of DNA in OA soils (mean = 22.1 ± 3.92 μg g −1), compared to CA soils (mean = 0.95 ± 0.17 μg g −1). While 16S metabarcoding evidenced the absence of significant differences among communities of the two farming systems in terms of ecological indices, the qPCR analyses targeting functional genes reported a significantly higher abundance of all considered targets in OA sites spanning up to four-fold log increases. While OA and CA did not appear to affect overall bacterial diversity or evenness per se, qPCR-based functional analysis in OA showed a consistently higher abundance of all the salient microbial genes tested, when compared to CA, underlying a potentially beneficial impact on soil fertility and sustainability. Conclusions In essence, the sequencing-based analysis of absolute bacterial diversity could not differentiate the farming systems based on the amount of diversity but identified a unique set of taxa defining each. Hence, pairing this evaluation with the qPCR-based functional gene analyses can be a suitable approach to distinguish the exerted effects of CA or OA on soils.
Journal of Applied Phycology, Oct 14, 2017
The characterization of nutrient and biostimulant effects in crops is complex and needs rigorous ... more The characterization of nutrient and biostimulant effects in crops is complex and needs rigorous evaluations. In this study, we evaluated morphological and molecular responses induced by microalgae (Chlorella vulgaris and Scenedesmus quadricauda) extracts in Beta vulgaris L. The two microalgae extracts were firstly characterized by CNS, Fourier transform infrared spectroscopic analysis (FT-IR), and carbon-13 nuclear magnetic resonance (13 C NMR). Seedlings were grown in Hoagland's solution under controlled conditions. After 5 days of growth, 2 mL L −1 (1 mg C org L −1) and 4 mL L −1 (2 mg C org L −1) of the two microalgae extracts were added to the Hoagland solution. Roots were sampled 36 h after treatments. Inductively coupled plasma spectrometry (ICP-OES) and nanofluidic real-time PCR (OpenArray system) were used for sample profiling. Fifty-three sugar beet genes putatively involved in sulfate starvation were tested in treated and untreated samples. Root morphological traits were measured by means of a scanner-based image analysis system. Multivariate statistical analysis revealed no significant changes in the ionomic profile of Hoagland's solutions treated with the two microalgae extracts with respect to that of the untreated solution. At the molecular level, microalgae extract supplies upregulated many of the evaluated genes. Functional categorization revealed these genes to be related to various biological pathways and processes including primary and secondary metabolism and intracellular transport. At the morphological level, the treated seedlings showed significantly higher values for root traits related to soil exploration and nutrient uptake, such as total root length, fine root length (diameter < 0.5 mm), and number of root tips, than the untreated plants. These data indicate that microalgae extracts have biostimulant effects on the expression of root traits and genes related to nutrient acquisition in sugar beet.
Molecular and ionomic responses of Solanum lycopersicum L. (cv. Micro-Tom) plants treated with a novel calcium-based plant biostimulant
Plant Gene
Una Strada Armentaria Dell’Età Del Bronzo Nelle Valli Grandi Veronesi: Il Contributo Delle Analisi Chimiche Dei Suoli
Effect of two different humic substances on some glycolytic enzymes in callus culture of Pinus laricio
Agricoltura e allevamento nell'agro orientale di Altinum: il caso di Ca' Tron
Since 2000 a multidisciplinary team of archaeologists, geologists and paleobotanists has been car... more Since 2000 a multidisciplinary team of archaeologists, geologists and paleobotanists has been carrying out joint investigations at Ca’ Tron, a 11 km2 estate located at the northern lagoon of Venice and included in the east countryside of the ancient Venetian-Roman Altinum. During the systematic survey conducted in Ca’ Tron estate nine Roman sites were identified, three of which were excavated in extension. Two rural settlements are analyzed in this paper, both attended since Ist to IV-V th century AD, having different planimetric features and economic role: the first one was a farm with several outbuildings, the second one was specialized in breeding sheep, with a large rectangular building, interpretable as a sheepfold on the basis of comparison with the Roman “bergeries” investigated in the Crau of Arles (Provence). Particularly relevant to the understanding of economic activities were the micromorphological analysis of soil types, chemical and biological, especially to clarify th...
Carbohydrate metabolism in Pinus laricio callus is affected by humic substances
Journal of Microbiology and Biotechnology, 2013
The fermented manure derivative known as Preparation 500 is traditionally used as a field spray i... more The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormonelike activity. The material was found to harbor a bacterial community of 2.38 × 10 8 CFU/g dw dominated by Grampositives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of β-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.
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Papers by Serenella Nardi