Papers by Solange I Mussatto
L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids
Frontiers in Bioengineering and Biotechnology
L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leuke... more L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leukemia. ASNase products available in the market are produced by bacteria and usually present allergic response and important toxicity effects to the patients. Production of ASNase by yeasts could be an alternative to overcome these problems since yeasts have better compatibility with the human system. Recently, it was found that Leucosporidium scottii, a psychrotolerant yeast, produces ASNase. In order to advance the production of ASNase by this yeast, the present study aimed to select suitable process conditions able to maximize the production of this enzyme in a bench-scale bioreactor. Additionally, the accumulation of lipids during the enzyme production process was also determined and quantified. Experiments were carried out with the aim of selecting the most appropriate conditions of initial cell concentration (1.0, 3.5, and 5.6 g L–1), carbon source (sucrose and glycerol, individually ...
Catalysts
This study aimed to select the best reaction conditions to produce 5-hydroxymethylfurfural (HMF) ... more This study aimed to select the best reaction conditions to produce 5-hydroxymethylfurfural (HMF) from cellulose using heterogeneous catalyst based on a heteropolyacid (H3PW12O40—HPW) and Nb2O5. Initially, the influence of the temperature (160 or 200 °C), acetone:water ratio (50:50 or 75:25 v/v), cellulose load (5% or 10% w/v) and catalyst concentration (1% or 5% w/v) on HMF production from cellulose was evaluated through a Taguchi’s L16 screening experimental design. Afterwards, the main variables affecting this process, namely the temperature (160–240 °C) and acetone:water ratio (60:40–90:10 v/v), were optimized using a central composite rotatable design. Next, a kinetic study on HMF production from cellulose was carried out. Finally, HMF production from cellulose obtained from different biomass sources was evaluated. It was found that the reaction conditions able to result in maximum HMF yield, i.e., around 20%, were 200 °C, acetone:water ratio of 75:25 (v/v), 10% w/v of cellulose...
Energies
Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approac... more Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% (w/v) initial solids (41.5 °C, 18 spheres). Then, assays were carried out at higher solid loadings (16% and 24% w/v). At 8% or 16% solids, the fermentation efficiency was similar (ηF~75%), but the ethanol volumetric productivity (QP) reduced from 1.50 to 1.14 g/L.h. By increasing the solids to 24%, the process was strongly affected (ηF = 40% and QP = 0.7 g/L.h). To overcome this drawback, three different feeding profiles of 24% pre-treated rice straw were investigated. Gradual feeding of the substrate (initial load of 16% with additions of 4% at 10 and 24 h) and an inoculum level of 3 g/L resulted in a high ethanol titer (52.3 g/L) with QP of 1...
Energies
This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a fe... more This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a feedstock for the production of itaconic acid by fermentation. Additionally, different process strategies were tested with the aim of selecting suitable conditions for an efficient production of itaconic acid by the fungus Aspergillus terreus. The feasibility of using cellulose pulp was demonstrated through assays that revealed the preference of the strain in using glucose as carbon source instead of xylose, mannose, sucrose or glycerol. Additionally, the cellulose pulp was easily digested by enzymes without requiring a previous step of pretreatment, producing a glucose-rich hydrolysate with a very low level of inhibitor compounds, suitable for use as a fermentation medium. Fermentation assays revealed that the technique used for sterilization of the hydrolysate (membrane filtration or autoclaving) had an important effect in its composition, especially on the nitrogen content, consequently...
Energies
This study aimed to evaluate the synthesis and application of heterogeneous catalysts based on he... more This study aimed to evaluate the synthesis and application of heterogeneous catalysts based on heteropolyacids for 5-hydroxymethylfurfural (HMF) production from glucose. Initially, assays were carried out in order to establish the most favorable catalyst synthesis conditions. For such purpose, calcination temperature (300 or 500 °C), type of support (Nb2O5 or Al2O3), and active phase (H3PW12O40—HPW or H3PMo12O40—HPMo) were tested and combined based on Taguchi’s L8 orthogonal array. As a result, HPW-Nb2O5 calcined at 300 °C was selected as it presented optimal HMF production performance (9.5% yield). Subsequently, the reaction conditions capable of maximizing HMF production from glucose using the selected catalyst were established. In these experiments, different temperatures (160 or 200 °C), acetone-to-water ratios (1:1 or 3:1 v/v), glucose concentrations (50 or 100 g/L), and catalyst concentrations (1 or 5% w/v) were evaluated according to a Taguchi’s L16 experimental design. The c...
Sugarcane bagasse hydrolysate as a potential feedstock for red pigment production by Monascus ruber
Food chemistry, Jan 15, 2018
Sugarcane bagasse (SCB) hydrolysate could be an interesting source for red pigment production by ... more Sugarcane bagasse (SCB) hydrolysate could be an interesting source for red pigment production by Monascus ruber Tieghem IOC 2225. The influence of different wavelength of light-emitting diode (LED) at 250 μmol.m-2.s-1 of photon flux density on red pigment production by M. ruber in glucose-based medium was evaluated. Then, SCB hydrolysate was used as carbon source under the previously selected light incidence conditions. In glucose-based medium, the highest pigment production was achieved in fermentation assisted with orange LED light (8.28 UA490nm), white light (8.26 UA490nm) and under dark condition (7.45 UA490nm). By using SCB hydrolysate-based medium, the highest red pigment production (18.71 AU490nm) was achieved under dark condition and the glucose and cellobiose present in the hydrolysate were metabolized. SCB enzymatic hydrolysate was demonstrated to be a promising carbon source for high thermal stability red pigment production (activation energy of 10.5 kcal.mol-1), turning ...
Hydrodynamic cavitation as a strategy to enhance the efficiency of lignocellulosic biomass pretreatment
Critical reviews in biotechnology, Jan 12, 2017
Hydrodynamic cavitation (HC) is a process technology with potential for application in different ... more Hydrodynamic cavitation (HC) is a process technology with potential for application in different areas including environmental, food processing, and biofuels production. Although HC is an undesirable phenomenon for hydraulic equipment, the net energy released during this process is enough to accelerate certain chemical reactions. The application of cavitation energy to enhance the efficiency of lignocellulosic biomass pretreatment is an interesting strategy proposed for integration in biorefineries for the production of bio-based products. Moreover, the use of an HC-assisted process was demonstrated as an attractive alternative when compared to other conventional pretreatment technologies. This is not only due to high pretreatment efficiency resulting in high enzymatic digestibility of carbohydrate fraction, but also, by its high energy efficiency, simple configuration, and construction of systems, besides the possibility of using on the large scale. This paper gives an overview reg...
Non-digestible oligosaccharides: A review
Carbohydrate Polymers, Apr 1, 2007
... beneficially affect the host health, and for this reason, the NDOs are considered as function... more ... beneficially affect the host health, and for this reason, the NDOs are considered as functional food (Rivero-Urgell and Santamaria-Orleans, 2001 and Roberfroid and Slavin, 2000), which can be defined as a food ingredient which affects physiological function (s) of the body in a ...
20Th European Biomass Conference and Exhibition, 2012
Olive tree pruning (OTP) and rapeseed straw (RS) are agricultural residues generated in significa... more Olive tree pruning (OTP) and rapeseed straw (RS) are agricultural residues generated in significant amounts in Europe, and their reuse has been strongly encouraged for economical and environmental reasons. In the case of olive tree pruning, over 4.8 million hectares are cultivated in Europe, most of them in the Mediterranean countries. Pruning operation generates every year a volume of lignocellulosisc residues estimated in 3000 kg/ha. The conversion of OTP into ethanol (Cara et al., 2008) and other chemical production (Castro et al., 2008) has been proposed. Rapeseed cultivation has increased in recent years due to growing demand for this feedstock for biodiesel production. Over 5 million ha of rapeseed plant were cultivated in Europe in 2009. Rapeseed straw has been proposed as raw material to produce bioethanol (Díaz et al., 2010; Castro et al., 2011). OTP and RS contain a significant fraction of hemicellulose, a heteropolymer composed by pentose and hexose sugars. Extraction of the sugars from these residues, and subsequent use of them in fermentative processes, could be an interesting alternative for their valorization since a variety of valuable compounds may be produced by bioconversion of sugars.
A cerveja é uma bebida que apresenta características desfavoráveis para a multiplicação de vários... more A cerveja é uma bebida que apresenta características desfavoráveis para a multiplicação de vários microrganismos, sendo reconhecida como um produto de considerável estabilidade microbiológica. Porém, algumas espécies de microrganismos, incluindo bactérias Gram-positivas (Lactobacillus e Pediococcus), Gram-negativas (Pectinatus e Megasphaera) e leveduras selvagens (Saccharomyces e não-Saccharomyces) são capazes de se multiplicar nesta bebida, conferindo características indesejáveis, tais como turbidez e mudanças sensoriais, as quais prejudicam a qualidade do produto final. Este fato tem motivado uma série de pesquisas visando ao desenvolvimento de métodos rápidos e específicos para a detecção dos microrganismos deteriorantes da cerveja. Apesar de a detecção desses microrganismos, por cultivo em meios de laboratório, nem sempre proporcionar a especificidade e sensibilidade requerida, o uso de diferentes meios seletivos e condições de incubação continua sendo o método preferido pelas cervejarias. O presente artigo apresenta uma revisão da literatura sobre os principais microrganismos deteriorantes da cerveja, incluindo as causas que favorecem sua multiplicação e os métodos que podem ser empregados para sua detecção.
Improvement on D-xylose to Xylitol Biotransformation by Candida guilliermondii Using Cells Permeabilized with Triton X-100 and Selected Process Conditions
Applied biochemistry and biotechnology, Jan 2, 2016
Cells of Candida guilliermondii permeabilized with Triton X-100 were able to efficiently produce ... more Cells of Candida guilliermondii permeabilized with Triton X-100 were able to efficiently produce xylitol from a medium composed only by D-xylose and MgCl2·6H2O in potassium phosphate buffer, at 35 °C and pH 6.5. Under these conditions, the results were similar to those obtained when cofactor and co-substrate or nutrients were added to the medium (about 95 % D-xylose was assimilated producing 42 g/L of xylitol, corresponding to 0.80 g/g yield and 2.65 g/L h volumetric productivity). Furthermore, the permeabilized cells kept the D-xylose assimilation in about 90 % and the xylitol production in approx. 40 g/L during three bioconversion cycles of 16 h each. These values are highly relevant when compared to others reported in the literature using enzyme technology and fermentative process, thereby demonstrating the effectiveness of the proposed method. The present study reveals that the use of permeabilized cells is an interesting alternative to obtain high xylitol productivity using low...
Biotech 2014 and 6th Czech Swiss Symposium With Exhibition, Jun 11, 2014
Application of xylitol in food formulations and benefits for health
8Th Isanh World Congress on Polyphenols Applications, Jun 5, 2014
Brazilian Journal of Food Technology, 2007
A cerveja é uma bebida que apresenta características desfavoráveis para a multiplicação de vários... more A cerveja é uma bebida que apresenta características desfavoráveis para a multiplicação de vários microrganismos, sendo reconhecida como um produto de considerável estabilidade microbiológica. Porém, algumas espécies de microrganismos, incluindo bactérias Gram-positivas (Lactobacillus e Pediococcus), Gram-negativas (Pectinatus e Megasphaera) e leveduras selvagens (Saccharomyces e não-Saccharomyces) são capazes de se multiplicar nesta bebida, conferindo características indesejáveis, tais como turbidez e mudanças sensoriais, as quais prejudicam a qualidade do produto final. Este fato tem motivado uma série de pesquisas visando ao desenvolvimento de métodos rápidos e específicos para a detecção dos microrganismos deteriorantes da cerveja. Apesar de a detecção desses microrganismos, por cultivo em meios de laboratório, nem sempre proporcionar a especificidade e sensibilidade requerida, o uso de diferentes meios seletivos e condições de incubação continua sendo o método preferido pelas cervejarias. O presente artigo apresenta uma revisão da literatura sobre os principais microrganismos deteriorantes da cerveja, incluindo as causas que favorecem sua multiplicação e os métodos que podem ser empregados para sua detecção.
Xiv Congreso Nacional De Biotecnologia Y Bioingenieria, 2011
Bioresource Technology, 2016
h i g h l i g h t s Aguamiel is a potential source for production of fructosyltransferases enzyme... more h i g h l i g h t s Aguamiel is a potential source for production of fructosyltransferases enzymes. FOS production in aguamiel as substrate is a viable alternative. Fructosyltransferase and FOS production by Solid State Fermentation is a viable and economic alternative.
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Papers by Solange I Mussatto