SCBT

Nanoenabled drug carriers are emerging as alternatives to conventional small-molecule drugs and their in-vitro biocompatibility evaluation with blood components is a necessary part of early preclinical development. In the present study, Chitosan-based bionanocomposites of sol-gel derived TiO 2 and SiO 2 were synthesised by the ex situ process. Samples were characterised by X-ray diffraction, Fourier transformed infrared spectrophotometer, transmission electron microscopy and selected area electron diffraction techniques. The analysis reveals the formation of single-phase oxides and their bionanocomposites. Haemolysis (destruction of red blood cell) study was performed by spectrophotometer to assess the haemocompatibility nature as a function of different incubation time. Experimental results reveal the percentage of haemolysis increase with the increase of the incubation time, but it was found to be <2%. This confirms our bionanocomposites are more haemocompatible as compared with respective nanocrystalline ceramics. Also, the interaction of our bionanocomposites with serum proteins at various incubation periods (1-24 h) were investigated using sodium dodecyl sulphate-polyacrylamide gel experiment, the obtained results were discussed in details.

Objectives: In this present work, we have compared titanium dioxide nanocrystals synthesized by both chemical method and biological method from Titanium tetra isopropoxide as precursor. The biological method was performed by using the aqueous root extract of Desmodium gangeticum and further they were characterized and checked for their biological activities. Methods: The TiO2 Results: The nanocrystals synthesized by biological method were found to have higher antioxidant potential, antimicrobial activity and phenolic content than the chemically synthesized ones. Evaluation of toxicity in cell line LLC-PK1 suggests that TiO nanocrystals were characterized by UV-Visible spectrophotometer for its maximum SPR peak, X-ray diffractometry for its crystalline size and shape, Fourier transform infrared spectroscopy for functional entity identification and Zeta potential for its colloidal stability. Antioxidant assay and antimicrobial test were performed to evaluate its biological behaviour. ...

Cardiac mitochondrial dysfunction is considered to be the main manifestation in the pathology of ischemia reperfusion injury, and by restoring its functional activity, hydrogen sulfide (H 2 S), a novel endogenous gaseotransmitter renders cardioprotection. Given that interfibrillar (IFM) and subsarcolemmal (SSM) mitochondria are the two main types in the heart, the present study investigates the specific H 2 Smediated action on IFM and SSM during ischemic reperfusion in the Langendorff rat heart model. Rats were randomly divided into five groups, namely normal, ischemic control, reperfusion control (I/R), ischemic post-conditioning (POC), and H 2 S post-conditioning (POC_H 2 S). In reperfusion control, cardiac contractility decreased, and lactate dehydrogenase, creatine kinase, and infracted size increased compared to both normal and ischemic group. In hearts post-conditioned with H 2 S and the classical method improved cardiac mechanical function and decreased cardiac markers in the perfusate and infarct size significantly. Both POC and POC_H 2 S exerts its cardioprotective effect of preserving the IFM, as evident by significant improvement in electron transport chain enzyme activities and mitochondrial respiration. The in vitro action of H 2 S on IFM and SSM from normal and I/R rat heart supports H 2 S and mediates cardioprotection via IFM preservation. Our study indicates that IFM play an important role in POC_H 2 S mediated cardioprotection from reperfusion injury.

High-pressure x-ray powder diffraction has been measured on the half doped rare earth manganite Nd 0:5 Ca 0:5 MnO 3 up to a pressure of 15 GPa. We report the presence of a quantifiable amount of shear distortion of the MnO 6 octahedra in Nd 0:5 Ca 0:5 MnO 3 at high pressures. The lattice strain of Nd 0:5 Ca 0:5 MnO 3 is minimal at a crossover pressure of p 7 GPa, with the same lattice strain above and below this pressure achieved by shear and Jahn-Teller –type distortions, respectively. The increase in shear strain with increasing pressure provides a mechanism for the insulating behavior of manganites at