JANET WILLIAMS

RIVERS STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY, Microbiology, Lecturer 1

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Janet Olufunmilayo Williams is an Environmental and Petroleum Microbiologist with research interest in remediation of polluted environments using microalgae,bacteria, fungi and microorganisms generally. She is involved in modeling of oil spill simulated environments, industrial microbiology, use of lipolytic microorganisms for remediation exercises and lots more....

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Performance Evaluation of Non-Saccharomyces in Wine Production

by Awengi Alabere and JANET WILLIAMS

South Asian Journal of Research in Microbiology, 2020

Wines with different tastes, aromas, colours and acceptability produced through the fermentative ... more Wines with different tastes, aromas, colours and acceptability produced through the fermentative action of yeasts are consumed worldwide Several studies have centred on the use of Saccharomyces sp. as the choice yeast for fermentation due to its desirable characteristics; however, in recent years, researches are being focused on alternatives to Saccharomyces sp. This study however was aimed at carrying out a performance evaluation of wine produced by the fermentative action of non-Saccharomyces (Meyerozyma guilliermondii and Pichia guilliermondii). The wines were produced using a mixture of pineapple juice and blended banana pulp as substrate.The fermentation process comprised of two set ups: one was fermented by Meyerozyma guilliermondii strain 1621 and the other was fermented by Pichia guilliermondii strain PAX-PAT 18S. An organoleptic (sensory) evaluation of both wines produced was carried out to ascertain the quality of the wines in comparison to a commercial wine. This was done...

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Ecotoxicological Evaluatation of Artisanal Effluents on Bacillus, Enterobacter, Amorphotheca, Cladosporium and Penicillium Species in Brackish Water

Microbiology Research Journal International

Aim: To assess the effect of the hydrocarbon discharges from the artisanal refineries on the comm... more Aim: To assess the effect of the hydrocarbon discharges from the artisanal refineries on the community structure of microbial mats in an aquatic environment Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: The microbial mats, surface water and sediments samples were collected from four hydrocarbon polluted stations (A, B, C and D) and a control sampling station in Yellow island (Iyalla kiri) in Degema Local Government Area, in Rivers state Nigeria. The samples were immediately transported with ice packs to the Microbiology Laboratory of Rivers State University, Port Harcourt. The study lasted from March 2020 to February 2021, covering both wet and dry seasons. Methodology: Different concentrations of fresh effluent (0, 1.625, 3.25, 6.5, 12.5, 25, 50 and 75%) were prepared in test tubes to final volume of 10ml. Each of the test tubes was inoculated with one milliliter (1ml) of the test organism. Fiv...

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Bacteriological Quality of Money from the Cash Registers of Banks and Markets in the Port Harcourt Metropolis and Bacterial Susceptibility to Antibiotics

South Asian Journal of Research in Microbiology

Bacterial contamination of money acts as vehicle for the transmission of pathogenic and drug resi... more Bacterial contamination of money acts as vehicle for the transmission of pathogenic and drug resistant organisms. This study was carried out to investigate the bacteriological quality and antibiotic susceptibility patterns of money bank cash-points and markets in Port Harcourt metropolis. Two hundred and eighty-eight (288) naira notes belonging to the following denominations, N5, N10, N20, N50, N100, N200, N500 and N1000 were collected for three months from banks and markets and subjected to standard microbiological procedures such as standard plate counts, identification, sensitivity testing using Kirby-Bauer disk diffusion method. Total heterotrophic bacterial count (THB) ranged from 0.6±0.00x106 to 12.80±9.19x106cfu/g; 0.65±0.21x106 to 13.05±9.55x106cfu/g; 0.65±0.20x106 to 8.05±2.48x106Cfu/g in N5 and N100 from Access Bank (AB), United Bank of Africa (UBA) and Guaranty Trust Bank (GTB), respectively. There was a significant difference (p≤0.05) in the THB between the different nai...

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Fungal Population and Physicochemical Characteristics of Abattoir-Impacted Soil in Iwofe, Rivers State

South Asian Journal of Research in Microbiology, 2021

Aim: To determine the fungal population and physicochemistry of abattoir impacted soil in Iwofe, ... more Aim: To determine the fungal population and physicochemistry of abattoir impacted soil in Iwofe, Rivers State. Study Design: This study focused on Abattoir impacted soil. Statistical analysis of data and interpretation was carried out. Place and Duration of Study: Abattoir impacted soil was collected from three points in an abattoir located in Iwofe, Rivers State while the unpolluted soil which served as control was collected from the Rivers State University, Port Harcourt in January, 2021. Methodology: Standard microbiological techniques were used: the fungal population was determined by inoculating aliquots of an appropriate dilution resulting from a ten-fold serial dilution on prepared Sabouraud dextrose agar plates in duplicates. Plates were later incubated for 3-5 days after which colonies were enumerated and used in obtaining the fungal population in the soil samples while distinct colonies were subcultured for macroscopic and microscopic identification of fungi. The physicoch...

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Assessment of Fungi Associated with Bakery Products in Port Harcourt Metropolis

Microbiology Research Journal International, 2020

Bakery products such as bread, cakes, etc are staple foods consumed by both the poor and rich. Br... more Bakery products such as bread, cakes, etc are staple foods consumed by both the poor and rich. Bread and other bakery products are subject to fungal contamination. This study is aimed at identifying the different fungi associated with bakery products in Port Harcourt metropolis, Nigeria. Sampling was done once monthly for three months, January to March for twenty-three (23) bakery products. Various bakery products were collected from different locations (Abacha Road, Ikwerre Road and Mile 3) in Port Harcourt Metropolis for enumeration, isolation and identification of different fungi that cause spoilage of the bakery products. In the month of January, bread samples had the following fungal counts: 2.0x10 6 sfu/g, 1.0 x10 6 sfu/g, 2.0x10 6 sfu/g, 1.0x10 6 sfu/g, 1.0x10sfu/g and 3.0x10sfu/g. The lowest count was 1.0 x10sfu/g and the highest count was 3.0 x10 6 sfu/g with mean values of 1.07±1.00 a and 2.5±0.55 a , respectively. During the study, ten (10) different species of fungi were...

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Trajectory of Medium Oil Spill in a Brackish Aquatic System

The trajectory of a simulated medium oil spill (930 m 3 ) in a brackish water system of 1.5m 2 wa... more The trajectory of a simulated medium oil spill (930 m 3 ) in a brackish water system of 1.5m 2 was investigated employing MapInfo GIS software and solid phase continuous flow system (SPCFS) for monitoring biodegradation. The objective of this study was to determine the path and speed a simulated oil spill could take during the rainy and dry seasons. From a hypothetical spill site with actual spill position on latitude 4 o 47 N and longitude 6 o 58 and 7000 ,the distance travelled from the point of spill to New Calabar River during the rainy season was 361.4 m and 467.0 m for the dry season. Biodegradation was monitored for a period of 90 days. Physicochemical parameters of the liquid phase of the SPCFS include pH 6.55 -7.14, dissolved oxygen 2.4 – 6.8mg/l and nitrate 2.65 – 6.53 mg/l. During the rainy season, the tributaries flowed North East and South West directions towards New Calabar River as a result of increase in volume due to rainfall but for the dry season, they maintained ...

Assimilation of Microplastics and Bacteria Associated with the Organs of Wild Mullet Harvested from an Estuary in Rivers State, Nigeria

This research investigates the assimilation ofmicropastics (MPs) and bacteria associated with int... more This research investigates the assimilation ofmicropastics (MPs) and bacteria associated with internal organs of wild Mullet (Liza grandisquamis)harvested from Ohiakwo estuary. The bacterial quality wasevaluated using standard microbiological and Fourier transform infrared (FTIR) spectroscopy analyses. The adult mullet samples used have supposedly acclimatized to the biogeochemical, stress and plastisphere ecology of theaquatic ecosystems. Bacterial group counts (BGCs) among the organs (gill, intestine and tissue) was high (10 7 -10 8 CFU/g) but varied slightly with the highest been total coliform counts, 2.33x10 8 CFU/g.The bacteria associated were identified from culture-dependent molecular techniquewith accession numbers asStaphylococcus epidermidis (AE015931), Pseudomonas xiamenensis (MH734834), Bacillus licheniformis (AF478085), Klebsiella pneumoniae (MH021669), Bacillus lentus (AB021189), Escherichia coli (KX609714) and Vibrio alginolyticus (MH734825) respectively. FTIR spectr...

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Performance Evaluation of Non-Saccharomyces in Wine Production

South Asian Journal of Research in Microbiology, 2020

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Bacteriological Analysis of Top Soil from an Electronic Waste Dumpsite in Port Harcourt Metropolis

South Asian Journal of Research in Microbiology, 2020

Aim: To determine the bacterial and physicochemical analysis of topsoil from an electronic waste ... more Aim: To determine the bacterial and physicochemical analysis of topsoil from an electronic waste (e-waste) dumpsite within Port Harcourt metropolis using standard procedures. Place and Duration of Study: The study area was Kaduna street, beside Fruit and Vegetable Garden Market, Mile l which is located in Port Harcourt, Rivers State, Nigeria. The coordinates are 4o47’57.5” N 7o00’02.7” E. The duration of study was between March and September, 2019. Methodology: The waste disposed were mainly television sets, computer monitors, radio sets, stoves, laptops and central processing units. Soil samples were cleared off top debris, collected within 5cm of the top soil from four (4) different points of the dumpsite and a control was collected from area devoid of waste disposal, 20m away from the dumpsite. The five samples were kept in clean sterile polythene bags. Contamination observed from soil samples was attributed to the waste disposal. The total heterotrophic bacterial count was perfo...

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Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

Journal of Advances in Microbiology, 2020

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cu... more This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l. The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the cont...

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Groundnut Shell Infusion Agar as a Culture Medium for Moulds

South Asian Journal of Research in Microbiology, 2019

Aim: To carry out a Comparative mould analysis using groundnut shell infusion agar (GSA) and pota... more Aim: To carry out a Comparative mould analysis using groundnut shell infusion agar (GSA) and potato dextrose agar (PDA), as the control. Study Design: Laboratory-experimental design was used in this study. Place and Duration of Study: Soil samples were obtained from three different locations (Garden soil beside Biology Main Laboratory, opposite Faculty of Law and Faculty of Agriculture) in Rivers State University, Port Harcourt Rivers State, Nigeria. The study was carried out for three (3) months at the Microbiology Laboratory, Rivers State University, Port Harcourt. Methodology: Groundnut Shell Infusion Agar (GSA) was prepared by weighing 28 g of blended gari and 15 g of agar powder into 1L of groundnut shells filtrate. Potato dextrose Agar (PDA), a conventional medium was prepared according to the manufacturer’s specifications. GSA was prepared by weighing 28 g of blended gari and 15 g of agar powder into 1L of groundnut shells filtrate. Potato dextrose agar, a conventional medium...

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Comparative Bioremediation Potentials of Pseudomonas aeruginosa KX828570 and Bacillus megaterium KY085976 on Polluted Terrestrial Soil Treated with Oil Spill dispersant

South Asian Journal of Research in Microbiology, 2019

Aim: To assess the bioremediation potential of Pseudomonas aeruginosa and Bacillus megaterium on ... more Aim: To assess the bioremediation potential of Pseudomonas aeruginosa and Bacillus megaterium on oil spill dispersant polluted terrestrial soil. Study Design: The study employs experimental design, statistical analysis of data and interpretation. Place and Duration of the Study: Polluted Terrestrial Soil (Ts) samples were collected from Kegbara-Dere community in Gokana Local Government Area of Rivers State with sterile shovel from three different spots at the same location and put in black polythene bags and transported to the microbiological laboratory within 24 hours for physicochemical and microbiological analyses. Oil spill dispersant (OSD/LT and OSD/Seacare) were obtained from Baker and Hughes Nigeria Limited in Rivers state, Nigeria. Methodology: Standard microbiological procedures were used to enumerate, isolate and identify the bacterial isolates. Pseudomonas aeruginosa and Bacillus megaterium in oil spill dispersants contaminated soil were monitored over a period of 1, 7, 1...

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Antibiogram of Bacteria Isolated in the Air of Some Public Toilets in Port Harcourt Metropolis, Rivers State, Nigeria

South Asian Journal of Research in Microbiology, 2019

Antimicrobial resistance is one of the major challenges facing the health sector. This study was ... more Antimicrobial resistance is one of the major challenges facing the health sector. This study was aimed at investigating the antimicrobial susceptibility pattern of bacterial isolates from indoor air of public toilets in motor parks with a view of developing an antibiogram. The study sites included the public toilets in Mile 3 Motor Park, Rivers Transport Company (RTC) park and a General Motor Park in Waterlines, Port Harcourt, Nigeria. The indoor air was sampled using the plate exposure and disc diffusion techniques in determining the antimicrobial susceptibility pattern of the bacterial isolates. The bacterial isolates in the genera, Staphylococcus, Bacillus, Providencia, Pseudomonas, Escherichia, Enterobacter and Klebsiella species were identified. The antibiogram of the bacteria isolated showed that the bacterial isolates exhibited multi-drug resistant species as the isolates were resistant to more than two antibiotics. Ciprofloxacin and Tarivid are the drug of choice and recomm...

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A Mathematical Model Incorporating the Influence of Biodegradation on the Fate of a Simulated Oil Spill in a Brackish Aquatic System

British Journal of Environment and Climate Change, 2012

Aims: To address the limitation in the Nigerian Oil Spill Model (NOSM) which models biodegradatio... more Aims: To address the limitation in the Nigerian Oil Spill Model (NOSM) which models biodegradation using the first order decay process in which the rate of oil biodegraded is proportional to the initial mass and an empirical decay coefficient. It does not monitor the total biomass of the microorganisms involved in biodegradation. It is not a reflection of natural systems which is a combination of first, second and third order reactions.

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Assessment of Microplastic Degrading Potential of Fungal Isolates from an Estuary in Rivers State, Nigeria

The potential of fungi as bio degraders of micro plastic particles was assessed using standard mi... more The potential of fungi as bio degraders of micro plastic particles was assessed using standard microbiological and Fourier transformed Infra-Red (FTIR) spectroscopic analysis methods. The highest mean Total Heterotrophic fungal (THF) count of 4.24x10 4 cfu/ml was obtained with the least THF (2.72x10 cfu/ml) recorded during the dry season. Mean hydrocarbon Utilizing Fungal (HUF) count was highest (1.78x10 4 cfu/ml) during the wet period while the least HUF count (1.46x10 cfu/ml) was recorded during the dry period. Spectra of FTIR showed that the water contained microplastic particles in these proportions; polyethylene of low density (LDPE) 0.01%, 0.11% protein, 0.15% polystyrene, 0.37% polyamide, 1.14% cellulose, 1.21% polyurethane, 1.90% polyvinyl chloride, 3.11% polyester and 92% polypropylene, respectively. Species of fungi identified were Aspergillus niger, Penicillium spp., Rhizopus spp., Mucor spp., Aspergillus nidulans, Fusarium spp., Microsporum canis, and Aspergillus fumigat...

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