Daniele Mauro

Queen Mary, University of London, Centre for Experimental Medicine and Rheumatology, Department Member

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Address: London, United Kingdom

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Inhibitory Effect of Thymol on Suicidal Erythrocyte Death

Cellular Physiology and Biochemistry, 2009

The antibacterial plant component thymol has antioxidant activity. Oxidative stress is known to a... more The antibacterial plant component thymol has antioxidant activity. Oxidative stress is known to activate Ca(2+)-permeable cation channels with subsequent Ca(2+) entry, activation of Ca(2+)-sensitive K(+) channels, cell shrinkage, cell membrane scrambling and phosphatidylserine exposure at the erythrocyte surface. Cell shrinkage and phosphatidylserine exposure are typical features of suicidal erythrocyte death or eryptosis. Eryptotic cells are cleared from circulating blood thus causing anemia and may adhere to the vascular wall thus interfering with the microcirculation. The present experiments explored whether thymol interacts with eryptosis. Annexin V-binding was utilized to determine phosphatidylserine exposure, forward scatter to detect alterations of cell volume and Fluo3 fluorescence to depict changes of the cytosolic Ca(2+) activity. Oxidative stress (30 min. 0.3 mM tert-butylhydroperoxide), energy depletion (48 h glucose removal) and isotonic cell shrinkage (48 h replacement of extracellular Cl(-) with gluconate) significantly increased annexin V-binding and decreased the forward scatter, effects significantly blunted in the presence of thymol 2.5 - 20 microg/ml. Thymol is a potent inhibitor of suicidal erythrocyte death particularly following oxidative stress. In conclusion, thymol may be useful incounteracting anemia and impairment of microcirculation.

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Improvement in insulin resistance after short-term treatment with abatacept: case report and short review

by Denis Gagliardi, Daniele Mauro, Rosa Daniela Grembiale, and Francesco Ursini

Clinical Rheumatology, 2012

Insulin resistance, a key feature of type 2 diabetes

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Triggering of Suicidal Erythrocyte Death by Amphotericin B

Cell Physiol …, 2009

Amphotericin B is widely used as antifungal drug. Side effects include anemia. A variety of drugs... more Amphotericin B is widely used as antifungal drug. Side effects include anemia. A variety of drugs and diseases associated with anemia has recently been shown to trigger suicidal erythrocyte death or eryptosis, i.e. cell membrane scrambling and cell shrinkage. Eryptosis may be triggered by increased cytosolic Ca 2+ activity and by lack of ATP. The present study explored whether amphotericin B stimulates eryptosis. Cell membrane scrambling was estimated from annexin V-binding to phosphatidylserine exposed at the cell surface, cell shrinkage from forward scatter in FACS analysis, cytosolic Ca 2+ activity from Fluo3 fluorescence and the cytosolic ATP concentration from a luciferase-based assay. Exposure to amphotericin B (0.1 -1 µg/ml) within 48 hours significantly increased annexin V-binding, decreased forward scatter, increased cytosolic Ca 2+ activity and decreased cytosolic ATP content. In conclusion, amphotericin B stimulates suicidal cell death of erythrocytes, which may in turn contribute to the clearance of circulating erythrocytes and thus to anemia.

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“Blockade of Nitric Oxide Overproduction and Oxidative Stress by Nigella Sativa Oil Attenuates Morphine-Induced Tolerance” by Abdel-Zaher et al

Neurochemical Research, 2011

... 7. Moran MM, McFarland K, Melendez RI, Kalivas PW, Seamans JK (2005) Cystine/glutamate exchan... more ... 7. Moran MM, McFarland K, Melendez RI, Kalivas PW, Seamans JK (2005) Cystine/glutamate exchange regulates metabotropic glutamate receptor presynaptic ... 6393 8. LaRowe SD, Mardikian P, Malcolm R, Myrick H, Kalivas P, McFarland K, Saladin M, McRae A, Brady K (2006 ...

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Daniele Mauro

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