Papers by Olaoluwa Akinwale
Human cytomegalovirus infection in Nigerians living with human immunodeficiency virus
Annals of Tropical Medicine and Public Health, 2013
Living conditions and public health status in three urban slums of Lagos, Nigeria
South East Asia Journal of Public Health, 2013
Survey of hypertension, diabetes and obesity in three Nigerian urban slums
Iranian Journal of Public Health , 2013
Benefits of using community-engaged research approach to promote healthy lifestyles in three Nigerian urban slums
Southern African Journal of Epidemiology and Infection, 2013
Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by PCR amplification of a species specific mitochondrial gene
Tropical Parasitology, 2014
Parasites & Vectors, 2010
Background: Studies on biting behaviours and infectivity status of insect vectors are pre-requisi... more Background: Studies on biting behaviours and infectivity status of insect vectors are pre-requisites in understanding the epidemiology of the vector-borne diseases and planning effective control measures. A longitudinal study was carried out to investigate the transmission index of Simulium damnosum complex species along Osun River, South Western Nigeria. Adult flies were collected on human attractants from 07:00 to 18:00 hours for two consecutive days from February 2008 to June 2009 at three communities: Osun Eleja, Osun Ogbere and Osun Budepo. The infectivity rate was determined by dissection and Polymerase Chain Reaction amplification (PCR) of 0-150 genes of Onchocerca parasite using the pool screening technique.
Veterinary Parasitology, 2006
Sahel goats, also known as Borno whites are found in the northern semi-arid, tsetse free Sahel re... more Sahel goats, also known as Borno whites are found in the northern semi-arid, tsetse free Sahel region of Nigeria. They are transported alongside cattle from this zone to all other zones in the country, including the tsetse-infested zones, for commercial purposes and are kept for some time in these tsetse-infested zones until they are sold. This study therefore assessed the susceptibility of this breed of goats to trypanosome infection and its response to treatment with Berenil. Six bucks were inoculated intravenously with Trypanosoma vivax through the jugular vein while two served as uninfected control. The mean pre-patent period was 4.5 days and increasing parasitaemia followed the establishment of infection. Onset of parasitaemia was associated with increase in rectal temperature in all the infected goats and the temperature peak coincided with the only parasitaemic peak second week post-infection. The infected goats were treated with Berenil (Hoechst, Germany) 3.5 mg/kg body weight at 4 weeks post-infection. The packed cell volume (PCV) continued to fall from a mean 30.73 AE 0.01% preinfection to a mean 13.21 AE 0.18% at 1 week post-treatment. Deaths were recorded for 4 of the infected goats 1 week posttreatment while the remaining two died 2 weeks post-treatment, not responding to treatment. #
Acta Tropica, 2005
Schistosomiasis is hyperendemic in Nigeria. The national policy on schistosomiasis control has ad... more Schistosomiasis is hyperendemic in Nigeria. The national policy on schistosomiasis control has adopted praziquantel as the main drug of use in the control strategy aimed at reducing morbidity. However, the best delivery channel for praziquantel in schistosomiasis control in Nigeria is yet to be determined. This study assessed different channels for praziquantel delivery in a mass treatment effort. Three groups, each of two communities, were assigned to one of the following three channels: health facility (HF), school, and community for mass delivery of praziquantel to school-aged children. Distributors were trained on all aspects of praziquantel distribution and sufficient praziquantel tablets were provided for the drug distribution within 3 weeks following which an evaluation of the drug distribution by the different treatment channels was conducted. Overall, the community channel of delivery achieved the best coverage of 72.2% (range: 69-73%) compared to 44.3% (range: 39.5-62%) and 28.5% (range: 26.3-74.5%) for the HF and school channels, respectively. Community channel of praziquantel delivery as shown in this study ensures good coverage of both in and out-of-school children.
Detection of schistosomes polymerase chain reaction amplified DNA by oligochromatographic dipstick
Molecular and Biochemical Parasitology, 2008
The applications of highly specific and sensitive molecular techniques based on polymerase chain ... more The applications of highly specific and sensitive molecular techniques based on polymerase chain reaction (PCR) have constituted a valuable tool for the diagnosis of schistosomiasis and also for the detection of schistosome infections in the snail intermediate hosts. The common method of detecting PCR amplicons is gel electrophoresis in the presence of ethidium bromide, a carcinogen, which is followed by UV transillumination. Other methods, which are available for detecting PCR products, are real-time PCR, PCR-enzyme-linked immunosorbent assay (PCR-ELIZA) and mass spectrometry but they are cumbersome while they are sometimes complex and expensive. Therefore, a simple method of PCR product detection would be a welcome idea and a most valuable tool particularly in disease endemic countries with limited research facilities and resources. In this study, we applied a simple and rapid method for the detection of Schistosoma haematobium and Schistosoma mansoni PCR amplified DNA products using oligochromatographic (OC) dipstick. The amplicons are visualized by hybridization with a gold conjugated probe, while a control for the chromatographic migration is incorporated in the assay. The lower detection limit observed was 10fg of genomic DNA from each of the two species, while the dipstick was also specific for each of the species used in this study.
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Papers by Olaoluwa Akinwale