Papers by Muhammad Tayyib Naseem
DNA barcoding is highly effective for identifying specimens once a reference sequence library is ... more DNA barcoding is highly effective for identifying specimens once a reference sequence library is available for the species assemblage targeted for analysis. Despite the great need for an improved capacity to identify the insect pests of crops, the use of DNA barcoding is constrained by the lack of a well-parameterized reference library. The current study begins to address this limitation by developing a DNA barcode reference library for the pest aphids of Pakistan. It also examines the affinities of these species with conspecific populations from other geographic regions based on both conventional taxonomy and Barcode Index Numbers (BINs). A total of 809 aphids were collected from a range of plant species at sites across Pakistan. Morphological study and DNA barcoding allowed 774 specimens to be identified to one of 42 species while the others were placed to a genus or subfamily. Sequences obtained from these specimens were assigned to 52 BINs whose monophyly were supported by neighbor-joining (NJ) clustering and Bayesian inference. The 42 species were assigned to 41 BINs with 38 showing BIN concordance. These species were represented on BOLD by 7,870 records from 69 countries. Combining these records with those from Pakistan produced 60 BINs with 12 species showing a BIN split and three a BIN merger. Geo-distance correlations showed that intraspecific divergence values for 49% of the species were not affected by the distance between populations. Forty four of the 52 BINs from Pakistan had counterparts in 73 countries across six continents, documenting the broad distributions of pest aphids.
SUMMARY Co-infection of Carrot red leaf virus (CtRLV), Carrot mottle virus (CMoV) and Carrot red ... more SUMMARY Co-infection of Carrot red leaf virus (CtRLV), Carrot mottle virus (CMoV) and Carrot red leaf virus associated RNA (CtRLVaRNA) causes Carrot motley dwarf (CMD) disease. This study examined the capacity of the aphid Myzus persicae at transmitting viruses associated with CMD. M. persicae exposed to CMD-infected chervil plants transmitted CtRLV-, CMoV-and CtRLVaRNA to disease-free chervil, fennel, celery, carrot, cilantro, and parsley, as shown by RT-PCR using specific primers. Recipient plants developed typical CMD symptoms. Sequence analysis of the amplified virus genes showed high sequence diversity with corresponding sequences available in GenBank. This study expands on Cavariella aegopodii, the only previously recognized aphid vector of CMD-causing viruses.
Alps, the Central Midlands, the Hungarian Plains, the Western Dinaric Balcan, Italy (Southern Alp... more Alps, the Central Midlands, the Hungarian Plains, the Western Dinaric Balcan, Italy (Southern Alps), and the Carpathians.(Sub-) endemic species are concentrated at the south-eastern margins of the Alps indicating the effects of earlier glaciation processes. Most species are crenal or rhithral preferring elements but some potamobiont Perlid-and Perlodid-taxa, which are extremely rare or even extinct in most of Europe still live in the south-eastern plains of Austria.
Journal of Stored Products and …, Jan 1, 2011
Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) is one of the most prevalent stored-produc... more Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) is one of the most prevalent stored-product insect pests in Pakistan. It feed on those grains only, which have already been damaged by primary pest. To check the efficacy of different essential oil repellency behavior, Experiment was conducted in to determine the efficacy of oil extracted from extraction of Eucalyptus camaldulensis and Piper nigrum in various concentrations that is 20, 40 and 60% for the suppression control of red flour beetle under laboratory conditions. The incubator was maintained at 30 ± 2°C and 65 ± 5% R.H. The experiment was laid in completely randomize design. The results showed that higher concentration of plant extracted is in the form of essential oils resulted in maximum repellency at maximum exposure period as compared to minimum oil concentrations at minimum exposure period. Furthermore, it was also observed that E. camaldulensis proved more effective as compared to P. nigrum at all the concentrations as well as at all the post treatment interval.
Uploads
Papers by Muhammad Tayyib Naseem