raman thakur

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Bose Institute

Universiti Malaya

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Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi

Biotechnology Research International, 2015

Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To over... more Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To overcome stress, organism expresses heat-shock proteins (Hsps) or chaperons to perform biological function. Hsps are involved in various routine biological processes such as transcription, translation and posttranslational modifications, protein folding, and aggregation and disaggregation of proteins. Thus, it is important to understand holistic role of Hsps in response to stress and other biological conditions in fungi. Hsp104, Hsp70, and Hsp40 are found predominant in replication and Hsp90 is found in transcriptional and posttranscriptional process. Hsp90 and Hsp70 in combination or alone play a major role in morphogenesis and dimorphism. Heat stress in fungi expresses Hsp60, Hsp90, Hsp104, Hsp30, and Hsp10 proteins, whereas expression of Hsp12 protein was observed in response to cold stress. Hsp30, Hsp70, and Hsp90 proteins showed expression in response to pH stress. Osmotic stress is con...

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In silico Identification of Potential Peptides or Allergen Shot Candidates Against Aspergillus fumigatus

by raman thakur and Shankar Jata

BioResearch Open Access, 2016

Aspergillus fumigatus is capable of causing invasive aspergillosis or acute bronchopulmonary aspe... more Aspergillus fumigatus is capable of causing invasive aspergillosis or acute bronchopulmonary aspergillosis, and the current situation is alarming. There are no vaccine or allergen shots available for Aspergillus-induced allergies. Thus, a novel approach in designing of an effective vaccine or allergen shot candidate against A. fumigatus is needed. Using immunoinformatics approaches from the characterized A. fumigatus allergens, we have mapped epitopic regions to predict potential peptides that elicit both Aspergillus-specific T cells and B cell immune response. Experimentally derived immunodominant allergens were retrieved from www.allergen.org. A total of 23 allergenic proteins of A. fumigatus were retrieved. Out of 23 allergenic proteins, 13 of them showed high sequence similarity to both human and mouse counterparts and thus were eliminated from analysis due to possible cross-reactivity. Remaining allergens were subjected to T cell (major histocompatibility complex class I and II alleles) and B cell epitope prediction using immune epitope database analysis resource. Only five allergens have shown a common B and T cell epitopic region between human and mouse. They are Asp f1 {147-156 region (RVIYTYPNKV); Mito-gillin}, Asp f2 {5-19 region (LRLAVLLPLAAPLVA); Hypothetical protein}, Asp f5 {305-322 region (LNNYRPSSSSLSFKY); Metalloprotease}, Asp f17 {98-106 region (AANAGGTVY); Hypothetical protein}, and Asp f34 {74-82 region (YIQDGSLYL); PhiA cell wall protein}. The epitopic region from these five allergenic proteins showed potential for development of single peptide-or multipeptide-based vaccine or allergen shots for experimental prioritization.

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A698. Hip Arthroplasty for Failed Trochanteric Fracture Fixation

Journal of Bone Joint Surgery British Volume, Nov 1, 2011

Failure of internal fixation of intertrochanteric fractures is associated with delayed union or m... more Failure of internal fixation of intertrochanteric fractures is associated with delayed union or malunion resulting in persistent pain and diminished function. We evaluated 15 elderly patients treated with a tapered, fluted, modular, distally fixing cementless stem. At an average follow up of 2.86 years, mean Harris hip score improved from 35.90 preoperatively to 83.01 (P

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Spontaneous Displacement of Olecranon Fracture Through Geode Salvaged by Elbow Replacement

Rheumatology international, 2010

We present a case of pathological fracture of olecranon through a giant geode. Fracture was initi... more We present a case of pathological fracture of olecranon through a giant geode. Fracture was initially undisplaced and was treated conservatively. It later pro-gressed to a transolecranon dislocation as a result of a pseudarthrosis at the fracture site. The patient presented 4 ...

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