rachael duche

Follower

Following

Co-author

Public Views

Related Authors

National Dairy Research Institute

CINZIA LUCIA RANDAZZO

Dr. Anila Kumari

Mbarga Manga Joseph Arsene

People's Friendship University of Russia

Interests

Uploads

Papers by rachael duche

Surface Properties and Potential Immunomodulatory Effects of Candidate Probiotic Lactobacilli for Gastrointestinal Applications

Advanced Gut and Microbiome research, 2025

This study was aimed at evaluating the adhesive and immunomodulatory properties of indigenous lac... more This study was aimed at evaluating the adhesive and immunomodulatory properties of indigenous lactobacilli strains isolated from fermented foods, infant feces, and human milk. Methodology: The adhesive properties of lactobacilli strains were evaluated for hydrophobicity, coaggregation, and autoaggregation capacities. The best strains were evaluated for adhesion to HT-29 cell line in the presence of lipopolysaccharides. Further, the best performing strains were evaluated in post, pre, and coculture methods for modulation of cytokine release and their expression in cell through RT-qPCR and ELISA, respectively. Results: The test lactobacilli strains showed strong affinity toward toluene compared to hexadecane. Seven of the strains, namely, 3ST3, 3ST7, 8BM6, 8ST7, BK5, GR27, and GR8, were strongly (> 60%) hydrophobic toward both the solvents. Strains belonging to the Lacticaseibacillus casei group (3ST5, 72%; PT1, 64.33%; and 3BM4, 60%), except Lacticaseibacillus paracasei BK5, showed promising autoaggregation. The majority of strains displayed marked coaggregation within the ranges of 50%-70%, significantly higher than probiotic Lacticaseibacillus rhamnosus GG (LGG) (33%). Qualitative adhesion revealed that strain Lactiplantibacillus pentosus 15ST2 exhibited the highest rate of adhesion (45.33%) around the edges and surface of the HT-29 cells. Most of the lactobacilli significantly (p < 0 05) inhibited Staphylococcus aureus by exclusion interference. During transcriptional studies, a significant upregulation in the expression of TLR2 and the proinflammatory marker TNF-α was observed for KN3 (14.08-fold) and the 3BM3 strain (7.76-fold) in the coculture model. While in pre-and postculture, no significant difference in the expression of TLR2, TNF-α, and IL-10 was recorded in comparison to LPS-stimulated cells. The proinflammatory cytokine IL-8 was upregulated during pre-and postculture conditions at both transcription and translation levels. Conclusion: The indigenous lactobacilli (15ST2, 3BM3, and KN3) strains effectively adhered to intestinal cell surface and thereby have shown their potential to modulate the production of proinflammatory cytokines at both transcription and translation levels, when introduced alongside pathogens or established before bacterial infection.

DownloadEdit

Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria

by rachael duche and Lewis Ezeogu

BMC Microbiology

Introduction Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in... more Introduction Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized. Objectives This study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles. Methods Susceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes. Results A variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded a...

DownloadEdit

Antibiotic Resistance in Potential Probiotic Lactobacillary Strains of Fermented Foods and Human Origin From Nigeria

Probiotic Lactobacillus strains are generally recognized as safe and are being used in several fo... more Probiotic Lactobacillus strains are generally recognized as safe and are being used in several food and pharma formulations. Antimicrobial resistance is of growing concern globally. The presence of antibiotic resistance traits in bacterial strains of food origin and their possible transmission via functional foods is increasingly being emphasized. To be adequately suitable for use in foods/feed production for human and animal consumption, probiotic strains need to be free from transferable antibiotic resistance traits. This study screened potential probiotic Lactobacillus strains for their phenotypic and genotypic antibiotic resistance (AR) profiles. A wide spectrum of susceptibility and resistance was observed against different antibiotic classes. LAB isolates irrespective of origin displayed marked resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides and methicillin among beta-lactams with few exceptions. In contrary, high sensitivity was recorded against ...

Edit

Milk-Derived Antimicrobial Peptides: Overview, Applications, and Future Perspectives

Probiotics and Antimicrobial Proteins

The growing consumer awareness towards healthy and safe food has reformed food processing strateg... more The growing consumer awareness towards healthy and safe food has reformed food processing strategies. Nowadays, food processors are aiming at natural, effective, safe, and low-cost substitutes for enhancing the shelf life of food products. Milk, besides being a rich source of nutrition for infants and adults, serves as a readily available source of precious functional peptides. Due to the existence of high genetic variability in milk proteins, there is a great possibility to get bioactive peptides with varied properties. Among other bioactive agents, milk-originated antimicrobial peptides (AMPs) are gaining interest as attractive and safe additive conferring extended shelf life to minimally processed foods. These peptides display broadspectrum antagonistic activity against bacteria, fungi, viruses, and protozoans. Microbial proteolytic activity, extracellular peptidases, food-grade enzymes, and recombinant DNA technology application are among few strategies to tailor specific peptides from milk and enhance their production. These bioprotective agents have a promising future in addressing the global concern of food safety along with the possibility to be incorporated into the food matrix without compromising overall consumer acceptance. Additionally, in conformity to the current consumer demands, these AMPs also possess functional properties needed for value addition. This review attempts to present the basic properties, synthesis approaches, action mechanism, current status, and prospects of antimicrobial peptide application in food, dairy, and pharma industry along with their role in ensuring the safety and health of consumers.

DownloadEdit

Bile salt hydrolase and cholesterol assimilation potential of lactobacilli fromNigerian fermented foods and human sources

Indian Journal of Dairy Science

Bile salt hydrolases (BSH) results in microbes releasing enzymes conferring physiological functio... more Bile salt hydrolases (BSH) results in microbes releasing enzymes conferring physiological functions for both the producing organisms and the host and subsequently lower serum cholesterol. In recent years, these attributes have generated interest towards screening of lactic acid bacteria (LAB) for functional food production. In this present study, lactobacilli (n=34) (13 L. casei, 6 L. paracasei, 11 L. brevis, 3 L. pentosus and 1 L. plantarum) from varied sources viz. Nigerian fermented foods and human specimens were screened qualitatively and quantitatively for BSH activity using the direct plate assay and cell free extracts (CFE) of overnight cultures. To establish a correlation between BSH and cholesterol reduction, 12 isolates displaying high precipitation over bile salt agar plates, and high BSH enzyme activity in CFE were selected for in vitro cholesterol reduction assay. Results showed that 58% of the isolates from humans, and 36% from fermented food origin showed larger zones...

Edit

rachael duche

Uploads

Papers by rachael duche

Log In