Mrl/mpj Mice Are Protected Against Acute Lung Injury
/ Thematic Poster Session / Tuesday, May 17/8:15 AM-4:30 PM / Area G, C74 ACUTE LUNG INJURY: DIVE... more / Thematic Poster Session / Tuesday, May 17/8:15 AM-4:30 PM / Area G, C74 ACUTE LUNG INJURY: DIVERSE NOVEL INSIGHTS ... Hall B (Upper Level), Colorado Convention Center ... Mrl/mpj Mice Are Protected Against Acute Lung Injury ... , R. Anthuvan , C. Luong , A. ...
Computational Binding Studies of Thiamine and Receptor Based Discovery of New Antiprion Compounds Against Prion Protein via Docking and Pharmacophore Modeling
Comparative analysis of prion proteins for evolutionarily diverse vertebrate species, polymorphic variants and mutants-Structure and essential dynamics
A simple and sensitive biosensor for rapid detection of nanoparticles in water
ABSTRACT Advances in nanoscience have led to a greater use of engineered nanoparticles (ENPs) in ... more ABSTRACT Advances in nanoscience have led to a greater use of engineered nanoparticles (ENPs) in numerous applications. Due to their small size and unique surface properties, ENPs have many desirable features. However, they also interact with living cells in potentially undesirable manners highlighting the need to develop improved detection systems to manage risks associated with their accidental occupational exposure or environmental release. However, the routine detection of ENPs has not yet been demonstrated, especially for aquatic environments. Using standard protein engineering techniques, we generated a protein-based biosensor that can sensitively detect negatively charged ENPs in aquatic matrices. In particular, we genetically engineered a green fluorescent protein with a poly-lysine tag (His-GFP-LYS) to facilitate its electrostatic interaction with commercially available negatively charged NPs. These 5–6-nm-sized NPs have metallic cores comprising gold, iron oxide, cerium oxide, and zinc oxide and are stabilized via poly-acrylic acid (PAA) coating. The interaction between the recombinant positively charged GFP and the PAA coating of the negatively charged NPs resulted in visually observable turbidity changes that were quantified using a portable spectrophotometer (NANODROP). These interactions were confirmed using dynamic light scattering and visualized using agarose native gel electrophoresis. This simple and portable system could detect ENPs resuspended in pure aqueous buffer (0.08 mg/L) and those resuspended in environmental matrices, such as pond water (0.6 mg/L). This detection system also sensed ENPs in the presence of moderate concentrations of natural organic matter that is ubiquitously present in surface waters. These results suggest that this biosensor system could be used for the routine, portable, and affordable detection of negatively-charged ENPs under environmentally relevant aquatic conditions.
Metabolomics approach reveals lipid and carbohydrate alterations in the urine of FVB/N female mice subcutaneously infected with RML and RML plus lipopolysaccharide
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Papers by David Wishart