As the epidemiological distribution of Candida species changed in recent years and also to decide... more As the epidemiological distribution of Candida species changed in recent years and also to decide proper antifungal agents, the rapid and reliable identification of Candida species is essential. The conventional methods are labour intensive and take longer time to identify the Candida species and to judge proper antifungal agent. The automated systems proved to be considerably expensive. Many workers in their studies have been shown that chromogenic agars can identify Candida clearly to species level based upon the characteristic coloured colonies. The present study is carried out to prove the utility of HiCrome Candida differential agar in identification of Candida species. A total of 127 Candida strains, including 77 C. albicans, 23 C. tropicalis, 18 C. parapsilosis and 9 C. krusei identified by standard techniques were subcultured on HiCrome Candida differential agar and then incubated at 37 0 c for 48 hours to study their characteristics. The HiCrome Candida differential agar sh...
As the epidemiological distribution of Candida species changed in recent years and also to decide... more As the epidemiological distribution of Candida species changed in recent years and also to decide proper antifungal agents, the rapid and reliable identification of Candida species is essential. The conventional methods are labour intensive and take longer time to identify the Candida species and to judge proper antifungal agent. The automated systems proved to be considerably expensive. Many workers in their studies have been shown that chromogenic agars can identify Candida clearly to species level based upon the characteristic coloured colonies. The present study is carried out to prove the utility of HiCrome Candida differential agar in identification of Candida species. A total of 127 Candida strains, including 77 C. albicans, 23 C. tropicalis, 18 C. parapsilosis and 9 C. krusei identified by standard techniques were subcultured on HiCrome Candida differential agar and then incubated at 37 0 c for 48 hours to study their characteristics. The HiCrome Candida differential agar sh...
Seroprevalence of Hepatitis B Surface Antigen Among Pregnant Women in Rural Based Teaching Hospital of Northern
Background: Hepatitis B virus infection is an important global health problem and India accounts ... more Background: Hepatitis B virus infection is an important global health problem and India accounts for 10–15 % of the entire pool of HBV carriers of the world. Hepatitis B virus infection during pregnancy is associated with a high risk of maternal complications and has been reported as a leading cause of maternal mortality. Furthermore, the strong possibility of vertical transmission lends importance to diagnosing acute or chronic hepatitis B virus infection in pregnant women and justifies mandatory antepartum serum HBsAg screening. Aims & Objectives: This study aimed to determine the seroprevalence of hepatitis B surface antigen among pregnant women. Materials and Methods: The present retrospective hospital record-based study was conducted at the rural tertiary care teaching hospital in northern Maharashtra, India, over a period of 4 & 1/2 years, from January 2010 to June 2014. The screening for HBsAg, in all the serum samples collected from pregnant women, was performed using commer...
Bacteriological and Haematological P rofile of Neonatal Sepsis in Rural Teaching Hospital in Central India
Aim: This study was aimed to determine the bacteriological profile with their antimicrobial susce... more Aim: This study was aimed to determine the bacteriological profile with their antimicrobial susceptibility as well as haematological profile related to neonatal sepsis. Materials and Methods: In this prospective study conducted during August 2012 to July 2014, the blood samples collected from total 405 newborns suggestive of suspected sepsis were subjected to blood culture and initial sepsis screen. The identification of causative organisms was carried out by standard microbiological tests and antibiotic sensitivity testing as per Clinical Laboratory Standards Institute (CLSI) guidelines. Results: Of the 405 neonates with suspected sepsis, 29.38% had positive blood culture among which Gram negative organisms (68.06%) predominated over Gram positive organisms (31.93%) with Klebsiella species (35.29%) as the commonest bacterial pathogens. Both Gram negative and Gram positive organisms exhibited high to considerable resistance to commonly used antibiotics and found susceptible to merop...
Seroprevalence of Hepatitis B in Male Blood Donors in Rural Teaching Hospital of Northern Maharashtra, India
Aim: This study was aimed to know the seroprevalence of hepatitis B infection in male blood donor... more Aim: This study was aimed to know the seroprevalence of hepatitis B infection in male blood donors. Material & Methods: The present retrospective hospital record-based study was conducted at the blood bank of a rural tertiary care teaching hospital in northern Maharashtra, India over a period of 4 &1/2 years from January 2010 to June 2014. All the blood units collected were screened for HBV, HCV, HIV 1 and 2, syphilis and malaria. The data of HBsAg alone was analyzed with chi square test and results were considered significant if P value was < 0.05. Results: The overall seroprevalence rate was 1.63% among the total 3920 blood donors included in this study. The correlations of seroprevalence rate among selected age groups and in voluntary and replacement blood donors were found to be statistically insignificant. Conclusion: The lower seroprevalence rate in this study, further recommends strict abiding to donor selection criteria, comprehensive screening of blood donors, better awa...
As the epidemiological distribution of Candida species changed in recent years and also to decide... more As the epidemiological distribution of Candida species changed in recent years and also to decide proper antifungal agents, the rapid and reliable identification of Candida species is essential. The conventional methods are labour intensive and take longer time to identify the Candida species and to judge proper antifungal agent. The automated systems proved to be considerably expensive. Many workers in their studies have been shown that chromogenic agars can identify Candida clearly to species level based upon the characteristic coloured colonies. The present study is carried out to prove the utility of HiCrome Candida differential agar in identification of Candida species. A total of 127 Candida strains, including 77 C. albicans, 23 C. tropicalis, 18 C. parapsilosis and 9 C. krusei identified by standard techniques were subcultured on HiCrome Candida differential agar and then incubated at 370 c for 48 hours to study their characteristics. The HiCrome Candida differential agar sho...
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Papers by Vijay Ambade