Papers by Teresinha Silva
Schistosomicidal effect of divaricatic acid from Canoparmelia texana (Lichen): In vitro evaluation and ultrastructural analysis against adult worms of Schistosoma mansoni
Acta Tropica, Oct 1, 2021
In this study we evaluated the in vitro effect of divaricatic acid against coupled worms of Schis... more In this study we evaluated the in vitro effect of divaricatic acid against coupled worms of Schistosoma mansoni. The schistosomicidal effect was evaluated through the bioassay of motility and mortality, cellular viability of the worms and ultrastructural analysis through Scanning Electron Microscopy. To evaluate the cytotoxicity of divaricatic acid, a cell viability assay was performed with human peripheral blood mononuclear cells. Divaricatic acid proved effect against S. mansoni after 3 hours of exposure. At the end of 24 h the concentrations of 100 - 200 μM presented lethality to the worms. Motility changes were observed at sublethal concentrations. The IC50 obtained by the cell viability assay for S. mansoni was 100.6 μM (96.24 - 105.2 μM). Extensive damage to the worm's tegument was observed such as peeling, erosion, bubbles, edema, damage and loss of tubercles and spines, fissures and tissue ruptures. No cytotoxicity was observed in human peripheral blood mononuclear cells. This report provides data showing the schistosomicidal effect of divaricatic acid on S. mansoni, causing death, motile changes and ultrastructural damage to worms. In addition, divaricatic acid was shown to be non-toxic to human peripheral blood mononuclear cells at concentrations effective on S. mansoni.
Molecules
Cymbopogon winterianus, known as “citronella grass”, is an important aromatic and medicinal tropi... more Cymbopogon winterianus, known as “citronella grass”, is an important aromatic and medicinal tropical herbaceous plant. The essential oil of C. winterianus (EOCw) is popularly used to play an important role in improving human health due to its potential as a bioactive component. The present study aimed to identify the components of the essential oil of C. winterianus and verify its leishmanicidal and trypanocidal potential, as well as the cytotoxicity in mammalian cells, in vitro. The EOCw had geraniol (42.13%), citronellal (17.31%), and citronellol (16.91%) as major constituents. The essential oil only exhibited significant cytotoxicity in mammalian fibroblasts at concentrations greater than 250 μg/mL, while regarding antipromastigote and antiepimastigote activities, they presented values considered clinically relevant, since both had LC50 < 62.5 μg/mL. It can be concluded that this is a pioneer study on the potential of the essential oil of C. winterianus and its use against the...
Research, Society and Development, Nov 24, 2021
Antioxidant and gastroprotective effects of the ethyl acetate extract from Stemodia maritima L. i... more Antioxidant and gastroprotective effects of the ethyl acetate extract from Stemodia maritima L. in ethanol-induced gastric ulcer model Efeitos antioxidante e gastroprotetor do extrato acetato de etila de Stemodia maritima L em modelo de úlcera gástrica induzida por etanol Efectos antioxidante y gastroprotector del extracto de acetato de etilo de Stemodia maritima L. en el modelo de úlcera gástrica inducida por etanol
Sorghum (<i>Sorghum bicolor</i> L.) as a potential source of bioactive substances and their biological properties
Critical Reviews in Food Science and Nutrition, Dec 1, 2020
Abstract Sorghum is the fifth cereal most produced in the world after wheat, rice, maize, and bar... more Abstract Sorghum is the fifth cereal most produced in the world after wheat, rice, maize, and barley. In some regions, this crop is replacing maize, due to its high yield, resistance to drought and heat. There are several varieties of sorghum, whose coloration varies from cream, lemon-yellow, red, and even black. Pigmented sorghum grain is a rich source of antioxidants like polyphenols, mainly tannins, which have multiple benefits on human health such as, antiproliferative properties associated with the prevention of certain cancers, antioxidant activities related to the prevention of associated diseases to oxidative stress, antimicrobial and anti-inflammatory effects, it also improves glucose metabolism. Despite having these types of compounds, it is not possible to assimilate them, their use in the food industry has been limited, since sorghum is considered a food of low nutritional value, due to the presence of anti-nutritional factors such as strong tannins which form complexes with proteins and iron, thus reducing their digestibility. Based on these restrictions that this product has had as food for humans, the analysis of this review emphasizes the valorization of sorghum as a source of bioactive substances and the importance they confer on human health because of the biological potential it has.
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Inflammation Research, Sep 28, 2012
The various extracts of Merremia tridentata were investigated for its anti-inflammatory and anti-... more The various extracts of Merremia tridentata were investigated for its anti-inflammatory and anti-arthritic activities in male albino rats. The antiinflammatory activity was carried out using carrageenan-induced rat paw oedema model and the anti-arthritic activity was carried out using complete Freund's adjuvant induced arthritis model. Indomethacin (10 mg/kg bw) was used as a standard drug. The ethanol extract of M. tridentata exhibited significant dose dependent activity in acute inflammation and the doses of 100 mg/kg bw and 200 mg/kg bw produced 38.3% and 42.8% inhibition respectively after 3 h as compared with that of the standard drug which showed 48.5% inhibition. In arthritis model, the doses of 100 mg/kg bw and 200 mg/kg bw of the ethanol extract produced 49.0% and 51.7% inhibition respectively after 19 days when compared with that of the standard drug (55.5%). Both doses of the ethanol extract of M. tridentata exhibit significant anti-inflammatory and anti-arthritic activities.
Latin American Journal of Pharmacy, 2011
This paper describes the cytotoxic and antitumoral activities of kaempferol 3-O-(6"-O-E-pcoumaroy... more This paper describes the cytotoxic and antitumoral activities of kaempferol 3-O-(6"-O-E-pcoumaroyl)-β-D-glucopyranoside (tiliroside), kaempferol 3,7-di-O-α-L-rhamnoside (dhiramnoside) and of the mixture of sitosteryl-3-O-β-D-glucopyranoside and stigmasteryl-3-O-β-D-glucopyranoside (GM) isolated of the Herissantia crispa. The compounds did not present cytotoxic activity against NCI-H292, HEp-2 and KB cells. In vivo, dhiramnoside did not present significant inhibitory activity of the growth of sarcoma 180 when compared with the control group; however, tiliroside and GM-treated animals showed a high inhibition rate in the growth of the tumor. Tiliroside inhibits significantly the growth of the carcinoma of Ehrlich. In conclusion, tiliroside exhibited promising antitumor effects without an expressive toxicity.
Avaliação da toxidade aguda e das atividades citotóxica, antimicrobiana e antiinflamatória de 7-aril-2,3-diidrotiazolo[3,2-a]pirimidin-5-ona-6-carbonitrila
Latin American Journal of Pharmacy, 2009
... 7-aril-2,3-diidrotiazolo[3,2-a]pirimidin-5-ona-6-carbonitrila Fernando AC VITAL 1, Sebastião ... more ... 7-aril-2,3-diidrotiazolo[3,2-a]pirimidin-5-ona-6-carbonitrila Fernando AC VITAL 1, Sebastião J. MELO 1*, Teresinha G. SILVA 1, Silene CARNEIRO 1, Janete M. ARAÚJO 1 &amp; Francisco JB MENDONÇA Jr 2 ... 25. Ram, VJ, RC Srimal, DS Kushwaha &amp; L. Mishra (1990) J. Prakt. ...
International Immunopharmacology, Nov 1, 2014
Diethylcarbamazine citrate (DEC) is widely used to treat lymphatic filariasis and Tropical Pulmon... more Diethylcarbamazine citrate (DEC) is widely used to treat lymphatic filariasis and Tropical Pulmonary Eosinophilia. A number of studies have reported a possible role in the host immune system, but exactly how DEC exerts this effect is still unknown. The present study reports the effects of DEC pretreatment on NF-κB regulation using the pleurisy model induced by carrageenan. Swiss male mice (Mus musculus) were divided into four experimental groups: control (SAL); carrageenan (CAR); diethylcarbamazine (DEC) and curcumin (CUR). The animals were pretreated with DEC (50 mg/kg, v.o), CUR (50 mg/kg, i.p) or distilled water for three consecutive days before pleurisy. One way analysis of variance (ANOVA) was performed by Tukey post-hoc test, and values were considered statistically significant when p b 0.05. DEC pretreatment reduced tissue damage and the production of inflammatory markers, such as NO, iNOS, PGE2, COX-2, and PARP induced by carrageenan. Similarly, a known inhibitor of NF-κB pathway (curcumin) was also able to reduce these parameters. Like curcumin, DEC prevents NF-κB activation by reducing NF-κB p65 phosphorylation and IκBα degradation. DEC prevented NF-κB activation via p38 MAPK, but did not interfere in the ERK pathway in this experimental model. However, further studies should be developed to confirm this hypothesis. These findings suggest that DEC could be a promising drug for inflammatory disorders, especially in pulmonary diseases such as Acute Lung Inflammation, due its high antiinflammatory potential which prevents NF-κB activation.
Acta Tropica, Apr 1, 2019
Usnic acid potassium salt from Cladonia substellata (Lichen): Synthesis, cytotoxicity and in vitr... more Usnic acid potassium salt from Cladonia substellata (Lichen): Synthesis, cytotoxicity and in vitro anthelmintic activity and ultrastructural analysis against adult worms of Schistosoma mansoni
Journal of Applied Microbiology, Dec 1, 2022
A new meroditerpene, sartorypyrone C (5), was isolated, together with the known tryptoquivalines ... more A new meroditerpene, sartorypyrone C (5), was isolated, together with the known tryptoquivalines L (1a), H (1b), F (1c), 3′-(4-oxoquinazolin-3-yl) spiro [1H-indole-3,5′]-2,2′-dione (2) and 4(3H)-quinazolinone (3), from the culture of the marine sponge-associated fungus Neosartorya paulistensis (KUFC 7897), while reexamination of the fractions remaining from a previous study of the culture of the diseased coral-derived fungus N. laciniosa (KUFC 7896) led to isolation of a new tryptoquivaline derivative tryptoquivaline T (1d). Compounds 1a-d, 2, 3, and 5, together with aszonapyrones A (4a) and B (4b), chevalones B (6) and C (7a), sartorypyrones B (7b) and A (8), were tested for OPEN ACCESS Mar. Drugs 2014, 12 823 their antibacterial activity against four reference strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa), as well as the environmental multidrug-resistant isolates. Only aszonapyrone A (4a) and sartorypyrone A (8) exhibited significant antibacterial activity as well as synergism with antibiotics against the Gram-positive multidrug-resistant strains. Antibiofilm assays of aszonapyrone A (4a) and sartorypyrone A (8) showed that practically no biofilm was formed in the presence of their 2× MIC and MIC. However, the presence of a sub-inhibitory concentration of ½ MIC of 4a and 8 was found to increase the biofilm production in both reference strain and the multidrug-resistant isolates of S. aureus.
Brazilian Journal of Infectious Diseases, 2013
Background: Sepsis is a systemic inflammation associated with infection caused by pathogenic micr... more Background: Sepsis is a systemic inflammation associated with infection caused by pathogenic microorganisms with high mortality rates. Objective: In this study, we investigated the protective effect of Propionibacterium acnes-killed against polymicrobial sepsis induced by cecal ligation and puncture. Methods: The mice were treated by intramuscular route in 1, 3, 5, and 7 days before the cecal ligation and puncture induction. The control group animals received vehicle (saline solution 0.9%) and the animals of the treated group received the P. acnes-killed (0.4 mg/animal). After anesthesia, midline laparotomy was performed with exposure of cecum followed by ligature and one transverse perforation of the same, with a 18 G needle, for induction of lethal sepsis. After surgery, the cecum of the animals was replaced into the peritoneal cavity, and it was closed with a 4.0 nylon suture. The survival of animals subjected to lethal sepsis was evaluated after cecal ligation and puncture induction. Six hours after the induction of sepsis, neutrophil migration, the number of bacteria, TNF-␣, MCP-1, IL-6, and IL-10 were performed in the peritoneal lavage. Results: Prophylactic treatment with P. acnes-killed increased the survival of the animals, followed by a significant decrease in the TNF-␣, IL-10, and MCP-1 levels, 6 h after cecal ligation and puncture. Furthermore, P. acnes-killed administration reduced the number of bacteria in the peritoneal cavity with increased migration of leukocytes, especially neutrophils. Conclusion: P. acnes-killed promoted increased survival rate of animals with sepsis, in part attributed to its immunomodulatory properties against pathogenic microorganisms, as well as better control of infection by reducing bacterial counts.
Zeitschrift für Naturforschung C, May 1, 2015
The chemical composition, antitumor activity and toxicity of the essential oil from Lippia microp... more The chemical composition, antitumor activity and toxicity of the essential oil from Lippia microphylla leaves (OEL) were investigated. The major constituents were thymol (46.5%), carvacrol (31.7%), p-cymene (9%), and γ-terpinene (2.9%). To evaluate the toxicity of OEL in non-tumor cells, the hemolytic assay with Swiss mice erythrocytes was performed. The concentration producing 50% hemolysis (HC 50) was 300 μg/mL. Sarcoma 180 tumor growth was inhibited in vivo 38% at 50 mg/kg, and 60% at 100 mg/kg, whereas 5-FU at 50 mg/kg caused 86% inhibition. OEL displays moderate gastrointestinal and hematological toxicity along with causing some alteration in liver function and morphology. However, the changes were considered reversible and negligible in comparison to the effects of several anticancer drugs. In summary, OEL displays in vivo antitumor activity and a moderate toxicity, which suggests further pharmacological study.
High doses of gamma radiation suppress allergic effect induced by food lectin
Radiation Physics and Chemistry, Apr 1, 2013
ABSTRACT One of the most promising areas for the development of functional foods lies in the deve... more ABSTRACT One of the most promising areas for the development of functional foods lies in the development of effective methods to reduce or eliminate food allergenicity, but few reports have summarized information concerning the progress made with food irradiation. In this study, we investigated the relationship between allergenicity and molecular structure of a food allergen after gamma irradiation and evaluate the profile of the allergic response to irradiated allergens. Cramoll, a lectin isolated from a bean and used as a food allergen, was irradiated and the possible structural changes were accompanied by spectrofluorimetry, circular dichroism and microcalorimetry. Subsequently, sensitized animals subjected to intragastric administration of non-irradiated and irradiated Cramoll were treated for 7 days. Then, body weight, leukocytes, cytokine profiles and histological parameters were also determined. Cramoll showed complete inhibition of intrinsic activity after high radiation doses. Changes in fluorescence and CD spectra with a simultaneous collapse of the tertiary structure followed by a pronounced decrease of native secondary structure were observed after irradiation. After oral challenge, sensitized mice demonstrate an association between Cramoll intake, body weight loss, eosinophilia, lymphocytic infiltrate in the gut and Eotaxin secretion. Irradiation significantly reduces, according to the dose, the effects observed by non-irradiated food allergens. We confirm that high-dose radiation may render protein food allergens innocuous by irreversibly compromising their molecular structure.
Toxicology and Applied Pharmacology, Apr 1, 2013
Thiazacridine derivatives (ATZD) are a novel class of cytotoxic agents that combine an acridine a... more Thiazacridine derivatives (ATZD) are a novel class of cytotoxic agents that combine an acridine and thiazolidine nucleus. In this study, the cytotoxic action of four ATZD were tested in human colon carcinoma HCT-8 cells: (5Z)-5-acridin-9-ylmethylene-3-(4-methylbenzyl)-thiazolidine-2,4-dione-AC-4; (5ZE)-5-acridin-9ylmethylene-3-(4-bromo-benzyl)-thiazolidine-2,4-dione-AC-7; (5Z)-5-(acridin-9-ylmethylene)-3-(4-chlorobenzyl)-1,3-thiazolidine-2,4-dione-AC-10; and (5ZE)-5-(acridin-9-ylmethylene)-3-(4-fluoro-benzyl)-1,3thiazolidine-2,4-dione-AC-23. All of the ATZD tested reduced the proliferation of HCT-8 cells in a concentration-and time-dependent manner. There were significant increases in internucleosomal DNA fragmentation without affecting membrane integrity. For morphological analyses, hematoxylin-eosin and acridine orange/ethidium bromide were used to stain HCT-8 cells treated with ATZD, which presented the typical hallmarks of apoptosis. ATZD also induced mitochondrial depolarisation and phosphatidylserine exposure and increased the activation of caspases 3/7 in HCT-8 cells, suggesting that this apoptotic cell death was caspase-dependent. In an assay using Saccharomyces cerevisiae mutants with defects in DNA topoisomerases 1 and 3, the ATZD showed enhanced activity, suggesting an interaction between ATZD and DNA topoisomerase enzyme activity. In addition, ATZD inhibited DNA topoisomerase I action in a cell-free system. Interestingly, these ATZD did not cause genotoxicity or inhibit the telomerase activity in human lymphocyte cultures at the experimental levels tested. In conclusion, the ATZD inhibited the DNA topoisomerase I activity and induced tumour cell death through apoptotic pathways.
Cytotoxic and leishmanicidal properties of garcinielliptone FC, a prenylated benzophenone from<i>Platonia insignis</i>
Natural Product Research, Mar 1, 2013
Garcinielliptone FC (GFC), a natural prenylated benzophenone, was extracted from Platonia insigni... more Garcinielliptone FC (GFC), a natural prenylated benzophenone, was extracted from Platonia insignis Mart. (Clusiaceae), a native plant commonly known as bacuri and used in traditional Brazilian medicine for the treatment of skin diseases. The aim of this study was to evaluate the cytotoxic and leishmanicidal effects of GFC using in vitro models. The experimental data demonstrated that the polyisoprenylated benzophenone GFC possesses cytotoxic and leishmanicidal activities.
Research, Society and Development, Aug 14, 2021
Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp... more Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp. isolated of the Amazon-Brazil region Atividades antioxidante, antimicrobiana e citotóxica de metabólitos secundários de Streptomyces sp. isolado da região Amazônica-Brasil Actividades antioxidante, antimicrobiana y citotóxica de los metabolitos secundarios de Streptomyces sp. aislado de la región Amazónica-Brasileña
Molecular Biology Reports, 2021
Secondary metabolites have been recognized for centuries as medicinal agents, in particular monot... more Secondary metabolites have been recognized for centuries as medicinal agents, in particular monoterpenes which have been the target of research in the discovery of antineoplastic drugs, as they have potential antitumor effect and low toxicity and are used as additives in foods and cosmetics. Another advantage of monoterpenes is structural diversity, which gives greater plasticity when interacting with cells. The purpose of this review was to summarize and critically discuss the anticancer potential of monoterpenes and their respective mechanisms of action. A systematic review of articles in the MEDLINE/ PubMed, Web of Science, Scopus and Science Direct electronic databases was independently conducted by three reviewers using the combination of the following keywords: monoterpenes AND anticancer AND in vitro. Restriction in selecting articles followed pre-established inclusion and exclusion criteria by the reviewers, and also a time limitation with works published between 2015 and 2019 being selected. In total, 39 works were deemed eligible for inclusion in the final review. Monoterpenes have cytotoxic activity in a wide variety of tumor cell lines, and mainly appear to exert this effect by inducing apoptosis caused by oxidative stress. In addition, improved use of monoterpenes when used in drug delivery systems and the synergistic effect with conventional chemotherapeutic drugs are reported. These findings validate this class of compounds as a promising source of chemotherapeutic drugs yet to be explored.
Evidence-Based Complementary and Alternative Medicine, 2020
Babassu (Attalea speciosaMart. ex Spreng., Arecaceae) is a palm tree endemic to Brazil and found ... more Babassu (Attalea speciosaMart. ex Spreng., Arecaceae) is a palm tree endemic to Brazil and found mainly in the borders of Amazon forest, where the harvesting of its fruits is an important source of income for more than 300,000 people. Among the communities of coconut breakers women, babassu oil is used in culinary, as fuel, and mostly as medicinal oil for the treatment of skin wounds and inflammation. This study aimed to evaluate in vitro and in vivo the wound healing effects of babassu oil. In vitro, babassu oil increased the migration of L929 fibroblasts, inhibited the production of nitric oxide by LPS-stimulated peritoneal macrophages, and increased the levels of INF-γand IL-6 cytokines production. In vivo, babassu oil accelerated the healing process in a full-thickness splinted wound model, by an increase in the fibroblasts number, blood vessels, and collagen deposition in the wounds. The babassu oil also increased the recruitment of inflammatory cells into the wound site and sh...
Applied Clay Science, 2019
The present research aimed at the synthesis of two new systems containing glibenclamide (GLIB) an... more The present research aimed at the synthesis of two new systems containing glibenclamide (GLIB) and double layered hydroxides (LDH). GLIB is an important drug used in the treatment of diabetes mellitus II (DM-II). The choice of zinc LDH (Zn 2 Al-Cl-GLIB) and magnesium (Mg 2 Al-Cl-GLIB) was performed with a possible antioxidant action, important for the treatment of DM II. The systems were characterized by x-ray diffractometry, medium infrared spectroscopy, surface area, thermogravimetry and differential exploratory analysis, mass analysis, chemical analyzes of carbon, nitrogen, and hydrogen (CHN) and metals (ICP-OES). The amount of GLIB carried by Mg 2 Al-Cl-GLIB was 0.625 mMol•g −1 and 0.666 mMol•g −1 by Zn 2 Al-Cl-GLIB. The release was performed by the dissolution test in simulated gastric and intestinal media. In the intestinal simulated medium LDH Zn 2 Al-Cl-GLIB and Mg 2 Al-Cl-GLIB showed a 13% and 30% solubility enhancement in relation to the isolated drug (GLIB), respectively. In addition, Zn 2 Al-Cl-GLIB presented antioxidant activity, suggesting potential action against free radicals produced in the chronic phase of DM-II. For both systems mesopore formation and absence of cellular toxicity were observed in macrophages. Glibenclamide (GLIB) is a second-generation sulfonylurea used to treat diabetes mellitus type II (DM-II). Due to its high affinity for pancreatic receptors and its potent ability to stimulate insulin secretion, i.e., secretagogue action, GLIB is greatly effective in clinical practice,
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Papers by Teresinha Silva