Saleem Javeed

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Phosphorus Adsorption as Described by Freundlich Adsorption Isotherms Under Rainfed Conditions of Pakistan

Soil phosphorus availability is the limiting factor for crop production in Pakistan. The objectiv... more Soil phosphorus availability is the limiting factor for crop production in Pakistan. The objective of the study was to investigate the phosphorus adsorption as described by Freundlich adsorption isotherm under rainfed conditions. Ten soil series of Pothwar Plateau were taken for study. These soils are treated with three different P fertilizers i.e DAP, SSP and NP at equilibrium solution concentration of 10, 20, 40, 60, and 80 µ g ml-1. A decrease in Padsorption with successive increase in equilibrium phosphorus solution concentration was recorded in all the soils under study. Maximum Freundlich adsorption parameters i.e maximum adsorption (µg g-1) and buffer capacity (ml g-1) were observed in Chakwal soil followed by Balkassar soil. The minimum values of these two Freundlich parameters were observed in Kahuta soil. Maximum value of K OC was observed in Chakwal soil with DAP, SSP and NP while minimum value of K OC was observed in Bather soil with all fertilizers under investigation. The minimum ∆G-20.19 KJ mol-1 ,-20.55 KJ mol-1 and-20.28 KJ mol-1 with DAP. SSP and NP, respectively was observed in Chakwal soil, while maximum values of ∆G was observed in Bather soil. The SSP showed maximum adsorption on the whole and DAP resulted in minimum adsorption of P fertilizer calculated by Freundlich adsorption isotherm. Buffer capacity was highest with SSP treatment and minimum with DAP fertilizer treatment in all ten soils under study. Further studies on P release pattern are suggested under rainfed conditions of Pakistan which would be important for plant nutrition.

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Suitability of soil types for Paecilomyces lilacinus and Pochonia chlamydosporia and their performance against root-knot nematode, Meloidogyne incognita on Lycopersicon esculentum in glass house

Indian Journal of Agricultural Sciences, 2013

During 2007-09, five broad soil types, black cotton, red laterite, alluvial, loamy sand and mount... more During 2007-09, five broad soil types, black cotton, red laterite, alluvial, loamy sand and mountain soils collected from Regional Centre, NBSSLUP, Bangalore, were examined for their suitability to NBAII isolates of antagonistic fungi, Paecilomyces lilacinus (Samson) Thomson and Pochonia chlamydosporia (Goddard) Zare, Gams and Evans in terms of their establishment; temporal survival and behaviour; pathogenicity to Meloidogyne incognita (Kofoid and White) Chitw. under glasshouse conditions and to arrive at the baseline data for field guidance. Except black cotton soil, other soil types examined were suitable for tomato root growth, root-knot nematode infection and effective parasitization of the egg masses by the fungi under report. The time-course behaviour of P. lilacinus and P. chlamydosporia in red laterite, alluvial, loamy and mountain soils were more or less similar. Both the fungi established in the first 2-3 weeks in soils tested, increased in number in the next 6-7 weeks wit...

Effect of inoculum morphology on production of Nigerloxin by solid state fermentation

Nigerloxin (2-amido-3-hydroxy-6-methoxy-5-methyle-4-(prop-1'enyl) benzoic acid) is a Lipoxygenase... more Nigerloxin (2-amido-3-hydroxy-6-methoxy-5-methyle-4-(prop-1'enyl) benzoic acid) is a Lipoxygenase and Aldose reductase inhibitor produced by Aspergillus niger MTCC 5116. It is produced only under solid state fermentation (SSF) and the inoculum required for the inhibitor production is developed in submerged condition. As this is a newly discovered enzyme inhibitor with potential commercial success against diabetic complications, such as neuropathy and cataract, its complete fermentation process parametric study is not reported. In this study, the role of physical parameters (spore suspension, initial pH, incubation temperature and agitation) in spore germination and pellet size in inoculum development broth in submerged fermentation for the enhanced production of nigerloxin through SSF has been studied. It was concluded that 500 µl of spore suspension in the inoculum development broth at pH 7, incubated at 30C and 200 rpm gave an ideal pellet size 1.23 mm resulting in 6.0 mg of nigerloxin/g dry weight of wheat bran in SSF.

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Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation

Journal of Industrial Microbiology & Biotechnology, 2009

Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through sol... more Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through solidstate fermentation of wheat bran by Aspergillus niger V. Teigh (MTCC-5166). The aim of this study is to investigate the possibility of using different agro-industrial residues as nutritional supplements along with wheat bran to enhance the production of nigerloxin. Nigerloxin produced by SSF was quantified spectrophotometrically at 292 nm. The results indicate that the inhibitor production was influenced by the type of solid substrate supplemented, moisture content, pH and size of the inoculum. Individually optimized supplements were tested in different combinations to determine their effects on nigerloxin production. A twofold increase in the production of nigerloxin (4.9 ± 0.3 mg gds-1) was achieved by supplementing wheat bran with 10% w/w sweet lemon peel and 5% v/w methanol at optimized process parameters, that is, an initial moisture content of 65% v/w and incubation period of 6 days with an initial inoculum size of 2 ml (8 9 10 5 spores gds-1). Nigerloxin production was stable between pH of 4 and 5.

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