SAMUEL WEMEDO

Effect of Oil Palm Bunch Ash (Elaeis Guineensis ) on the Bioremediation of Diesel Oil Polluted Soil

The physicochemical and microbiological characteristics of unpolluted soils, soils simulated with... more The physicochemical and microbiological characteristics of unpolluted soils, soils simulated with conditions of a major spill (diesel oil pollution) and soils treated with different weights of an organic supplement (oil palm bunch ash, OPBA) after simulation were investigated. Physicochemical parameters such as pH, water holding capacity and moisture contents decreased in both the unpolluted soils (control 1) and polluted soil without OPBA (control 2) throughout the study periods (Day 1 to Day 42). Addition of different weights of OPBA to the polluted soils caused an initial increase in pH, water holding capacity and moisture contents and decreased afterwards. Exchangeable cations also decreased in both the unpolluted soil and polluted soil without OPBA treatment. Treatment with OPBA increased nitrogen, phosphorus, calcium, magnesium, potassium and sodium contents. Both heterotrophic and diesel utilizing bacteria and fungi were isolated from unpolluted soil, polluted soil and OPBA t...

Download

The Inhibition Potentials of Different Honey against Staphylococcus aureus, Escherichia coli and Bacillus species Isolated from Clinical Source

International Journal of Pathogen Research, 2020

As a result of the increased prevalence of antibiotic resistance among different bacteria, differ... more As a result of the increased prevalence of antibiotic resistance among different bacteria, different plants and other natural products have been studied and found to be highly effective against pathogenic bacteria. Honey, over the years has been used as an antibacterial agent to treat certain infections caused by bacteria and is believed to be effective especially in rural areas. This study was thus aimed at comparing the effect of different honey samples against some pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Bacillus cereus) isolated from clinical source. This study was carried out in the microbiology laboratory, department of microbiology Rivers State University Nigeria from January 2018 to August 2019. The antibacterial sensitivity test was carried out using agar well diffusion method while the Minimum inhibitory concentration and Minimum bactericidal concentration were determined using broth tube micro dilution technique in two fold dilution. The inhibitio...

Download

Interaction Between Building Design and Indoor Airborne Microbial Load in Nigeria

Asian Journal of Biological Sciences, 2012

Biodegradation potential of Aspergillus Niger and Rhizopus arrhizus isolated from crude oil spilled site in Rivers State

Evaluation of biodegradation potentials of two fungal species (Aspergillus niger and Rhizopus arr... more Evaluation of biodegradation potentials of two fungal species (Aspergillus niger and Rhizopus arrhizus) isolated from a crude oil spilled site in Rivers State. The experimental set-up was done to determine degradation rates of fungal species during an incubation period of 28days. Total Petroleum Hydrocarbon content was taken on a bi-weekly basis while the Total Viable Count and pH readings were taken on a weekly basis respectively. Four experimental set-ups were used: mineral salt broth with crude oil (Control), Aspergillus species in mineral salt broth with crude oil (Set-up 1), Rhizopus species in mineral salt broth with crude oil (Set-up 2) and Aspergillus species +Rhizopus species in mineral salt broth with crude oil (Set-up 3) respectively. After the experiment there was an evident change in percentage degradation: Control 4.80%, Setup1 29.10%, Set-up2 26.32% and Set-up3 48%. The pH reading was observed to reduce from almost neutral to almost acidic: Control 6.4 to 4.8, Set-up1...

Download

Seasonal Influence on Microbial Load in Waste Engine Oil Polluted Soil of Automechanic Workshop in Port Harcourt Metropolis

Automechanic workshops are engine servicing centres and are source of automobile waste oil, the s... more Automechanic workshops are engine servicing centres and are source of automobile waste oil, the significant environmental impact associated with automechanic activities is the seepage of waste engine oil into the soil. This study was aimed at identifying the microbial species that are associated with waste engine oil polluted soil in automechanic workshops at Rumuoke and Elechi all in Port Harcourt City using molecular characterization. The soil samples were collected with the aid of a hand augar for microbiological analysis. The data obtained from this study were analyzed using analysis of variance (ANOVA) and Duncan Multiple using (DMRT). Based on Duncan multiple range test. The bacterial counts obtained during dry season ranged from 1.0x 10 4 cfu/g to 9.1x10 5 cfu/g while the rainy season were within the range of 1.0x10 4 cfu/g to 3.2x10 6 cfu/g. The fungal counts obtained during dry season ranged from 2.0x102cfu/g to 5.1x103cfu/g while the rainy season were within the range of 2...

Download

Ecotoxicological Evaluatation of Artisanal Effluents on Bacillus, Enterobacter, Amorphotheca, Cladosporium and Penicillium Species in Brackish Water

Microbiology Research Journal International

Aim: To assess the effect of the hydrocarbon discharges from the artisanal refineries on the comm... more Aim: To assess the effect of the hydrocarbon discharges from the artisanal refineries on the community structure of microbial mats in an aquatic environment Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: The microbial mats, surface water and sediments samples were collected from four hydrocarbon polluted stations (A, B, C and D) and a control sampling station in Yellow island (Iyalla kiri) in Degema Local Government Area, in Rivers state Nigeria. The samples were immediately transported with ice packs to the Microbiology Laboratory of Rivers State University, Port Harcourt. The study lasted from March 2020 to February 2021, covering both wet and dry seasons. Methodology: Different concentrations of fresh effluent (0, 1.625, 3.25, 6.5, 12.5, 25, 50 and 75%) were prepared in test tubes to final volume of 10ml. Each of the test tubes was inoculated with one milliliter (1ml) of the test organism. Fiv...

Download

Evaluation of the Bacteriological Diversity and the Physicochemical Properties of Kolo-Creek Oil Field Wastewater

Journal of Advances in Microbiology

Evaluation of bacteriological diversity and physicochemical parameters as well as the heavy metal... more Evaluation of bacteriological diversity and physicochemical parameters as well as the heavy metals present in the water samples collected from Kolo creek flow station located in Bayelsa State in the Niger Delta, Nigeria was carried out for the period of eight months (March to October). Oilfield wastewater samples were obtained in four different locations of the Kolo creek in sterile bottles and were transported to the Microbiology Laboratory of the Rivers State University for analysis.10-fold serial dilutions were carried out and aliquots of 0.1 ml were inoculated and spread plated unto sterile nutrient agar, MacConkey agar and mineral salt agar plates. The water samples were analyzed for the total heterotrophic bacteria, coliform bacteria and hydrocarbon utilizing bacteria. The total heterotrophic and coliform bacteria load in log10CFU/ml ranged from 6.25 to 13.62 and 4.04 to 5.75 respectively, while the hydrocarbon utilizing bacteria ranged from 0 to 4.81. Higher Mean monthly coun...

Evaluation of the Bacteriological Diversity and the Physicochemical Properties of Kolo-Creek Oil Field Wastewater

Journal of Advances in Microbiology

Evaluation of bacteriological diversity and physicochemical parameters as well as the heavy metal... more Evaluation of bacteriological diversity and physicochemical parameters as well as the heavy metals present in the water samples collected from Kolo creek flow station located in Bayelsa State in the Niger Delta, Nigeria was carried out for the period of eight months (March to October). Oilfield wastewater samples were obtained in four different locations of the Kolo creek in sterile bottles and were transported to the Microbiology Laboratory of the Rivers State University for analysis.10-fold serial dilutions were carried out and aliquots of 0.1 ml were inoculated and spread plated unto sterile nutrient agar, MacConkey agar and mineral salt agar plates. The water samples were analyzed for the total heterotrophic bacteria, coliform bacteria and hydrocarbon utilizing bacteria. The total heterotrophic and coliform bacteria load in log10CFU/ml ranged from 6.25 to 13.62 and 4.04 to 5.75 respectively, while the hydrocarbon utilizing bacteria ranged from 0 to 4.81. Higher Mean monthly coun...

Effect of Oil Palm Bunch Ash ( Elaeis Guineensis ) on the Bioremediation of Diesel Oil Polluted Soil

by SAMUEL WEMEDO, Chinomso Amajuoyi, and Ijeoma John

The physicochemical and microbiological characteristics of unpolluted soils, soils simulated with... more The physicochemical and microbiological characteristics of unpolluted soils, soils simulated with conditions of a major spill (diesel oil pollution) and soils treated with different weights of an organic supplement (oil palm bunch ash, OPBA) after simulation were investigated. Physicochemical parameters such as pH, water holding capacity and moisture contents decreased in both the unpolluted soils (control 1) and polluted soil without OPBA (control 2) throughout the study periods (Day 1 to Day 42). Addition of different weights of OPBA to the polluted soils caused an initial increase in pH, water holding capacity and moisture contents and decreased afterwards. Exchangeable cations also decreased in both the unpolluted soil and polluted soil without OPBA treatment. Treatment with OPBA increased nitrogen, phosphorus, calcium, magnesium, potassium and sodium contents. Both heterotrophic and diesel utilizing bacteria and fungi were isolated from unpolluted soil, polluted soil and OPBA treated soils. There was significant difference among controls and diesel oil polluted soils treated with different weights of OPBA for heterotrophic and diesel utilizing bacteria and fungi. Application of diesel oil and OPBA according to weight applied stimulated both bacteria and fungi which gradually declined with time as higher fractions of diesel oil were degraded. TPH ranges and percentage reduction observed throughout the experiment for all the treatments are as follows: US 16.96±1.27 to 11.21±2.47 (33.9%), PS 131.31±11.96 to 125.50±11.25 (4.4%), PS+50gOPBA 127.89±12.86 to 18.29±0.96 (85.7%), PS+100gOPBA 125.54±2.28 to 20.68±0.51 (83.5%), PS+200gOPBA 124.71±11.50 to 28.21±0.49 (77.38%) and PS+500gOPBA 124.04±5.02 to 36.25±0.38 (70.78%). Means of microbial counts are as follows: THB (×105 cfu/g) US 1.22±0.08, PS 0.9±0.06, PS+50gOPBA 16.99±0.0, PS+100gOPBA 18.79±0.0, PS+200gOPBA 21.70±0.0 and PS+500gOPBA 27.15±0.0. THF (×105 cfu/g) US 0.88±0.08, PS 0.33±0.06, PS+50gOPBA 15.70±0.0, PS+100gOPBA 17.32±0.0, PS+200gOPBA 20.38±0.0 and PS+500gOPBA 24.23±0.0. HUB (×105 cfu/g) US 0.93±0.08, PS 0.84±0.06, PS+50gOPBA 3.51±0.0, PS+100gOPBA 5.20±0.0, PS+200gOPBA 5.54±0.0 and PS+500gOPBA 6.58±0.0. HUF (×105 cfu/g) US 0.73±0.08, PS 0.48±0.06, PS+50gOPBA 2.81±0.0, PS+100gOPBA 3.70±0.0, PS+200gOPBA 4.02±0.0 and PS+500gOPBA 4.43±0.0. Microbial counts increased at the beginning of the experiment upon addition of OPBA and decreased with time. Bacterial and fungal species isolated were: Klebsiella sp, Acinetobacter sp, Streptomyces sp, Flavobacterium sp, Pseudomonas sp, Nocardia sp, Bacillus sp, Aerococcus sp, Alcaligenes sp and Micrococcus sp., Rhizopus sp, Aspergillus sp, Penicillium sp, Trichodema sp, Mucor sp, Fusarium sp and Cladosporium sp. OPBA enhanced oil degradation with %TPH reduction highest at PS+50gOPBA which is the optimal level likely to impact maximum economic gain.

Download

Uploads

Papers by SAMUEL WEMEDO

Log In