Papers by Rashmi Ranjan Mishra
World Journal of Agricultural Research, Jan 30, 2016
The present study was conducted to isolate phosphate solubilising bacteria from mangrove soils of... more The present study was conducted to isolate phosphate solubilising bacteria from mangrove soils of Mahanadi river delta, Odisha, India and evaluate their phosphate solubilising ability. In total forty-eight phosphate solubilising bacteria were isolated from different soil samples. Based on their size of halo zone formation on NBRIP agar medium and decrease in intensity of colour of the broth medium fourteen isolates were selected as efficient phosphate solubilising strains. Their efficiency on NBRIP agar medium were ranged from 108-175. Their ability to decrease the intensity of bluecolour of the NBRIP-BPB broth medium was ranged from 0.87 and 1.188 (O.D at 600nm). Phosphate solubilising ability test of these fourteen isolates showed that they can solubilise tricalciumphosphate from 8.21 to 48.70μg/ml and most of the isolates could acidified the medium supernatant below 4.0 from the initial pH 7.0. Morphological and biochemical characterisation of the isolates allowed us to identify them as members of the following genera: Pseudomonas, Bacillus, Alcaligens, Klebsiella, Serratia, Azotobacters and Micrococcus. All the fourteen PSB isolated from mangrove soil of Mahanadi river delta could efficiently solubilise tricalcium phosphate in the medium which could probably help for future application in biotechnology.
In the present study, physico-chemical parameters of water samples were compared with the water q... more In the present study, physico-chemical parameters of water samples were compared with the water quality standard of Bureau of Indian standard and the state pollution control board. Variations of different parameters investigated were as follows: Temperature (24.2-30.9°C), dissolved oxygen (2.9-10.9 mg/L), pH (6.05-8.6), electrical conductivity (5.16–17.33 mS/cm), TDS (4510–11900 mg/L), chloride content (4389-12575 mg/l), nitrate (13.03-24.01 mg/l), phosphate (0.55-2.59 mg/l), calcium (125.4-400.8 mg/l), magnesium (153.16-474.13 mg/l) and total hardness (800-2090 mg/l). The significant variations of p among different study sites with high load of calcium, chloride, nitrate and phosphate in most of the study sites indicating the pollution status of the estuarine water.
Annals of Microbiology, Jun 2, 2012
Mangrove forests occurring at the interface of terrestrial and marine ecosystems represent a rich... more Mangrove forests occurring at the interface of terrestrial and marine ecosystems represent a rich biological diversity of plants, animals and microorganisms. Microbes, being an important component of the mangrove environment, not only play a very critical role in creating and maintaining this biosphere but also serve as a source of biotechnologically valuable and important products. By participating in various steps of decomposition and mineralization of leaf litter, microbes make an essential contribution to the productivity of the mangrove ecosystem. They able to recycle nutrients, produce and consume gases that affect global climate, destroy pollutants, treat anthropogenic wastes and can also be used for biological control of plant and animal pests. Microorganisms from mangrove environments are a major source of antimicrobial agents and also produce a wide range of important medicinal compounds, including enzymes, antitumor agents, insecticides, vitamins, immunosuppressants, and immune modulators. However, the phylogenetic and functional description of microbial diversity in mangrove ecosystems has not been addressed to the same extent as for other environments. Even though the mangrove ecosystem is very rich in microbial diversity, less than 5% of species have been described; in many cases neither their ecological role nor their application potential is known. Recently developed technologies in molecular biology and genetics offer great promise to explore the potential of microbial diversity. Hence, the present paper makes an attempt to review the microbial diversity in mangrove ecosystems and explore their potential applications in various fields such as agriculture, pharmaceutical, industrial, environmental and medical sciences.
Revista De Biologia Tropical, Dec 5, 2012
Different groups of microorganisms are present in mangrove areas, and they perform complex intera... more Different groups of microorganisms are present in mangrove areas, and they perform complex interactions for nutrient and ecological balances. Since little is known about microbial populations in mangroves, this study analyzed the microbial community structure and function in relation to soil physico-chemical properties in Bhitarkanika, a tropical mangrove ecosystem in India. Spatial and seasonal fluctuations of thirteen important groups of microorganisms were evaluated from the mangrove forest sediments during different seasons, along with soil physico-chemical parameters. The overall microbial load (x10 5 cfu/g soil) in soil declined in the order of heterotrophic, free living N 2 fixing, Gram-negative nitrifying, sulphur oxidizing, Gram-positive, spore forming, denitrifying, anaerobic, phosphate solubilizing, cellulose degrading bacteria, fungi and actinomycetes. Populations of the heterotrophic, phosphate solubilizing, sulphur oxidizing bacteria and fungi were more represented in the rainy season, while, Gram-negative, Gram-positive, nitrifying, denitrifying, cellulose decomposing bacteria and actinomycetes in the winter season. The pool size of most of other microbes either declined or maintained throughout the season. Soil nutrients such as N, P, K (Kg/ha) and total C (%) contents were higher in the rainy season and they did not follow any common trend of changes throughout the study period. Soil pH and salinity (mS/cm) varied from 6-8 and 6.4-19.5, respectively, and they normally affected the microbial population dynamics. Determination of bacterial diversity in Bhitarkanika mangrove soil by culture method showed the predominance of bacterial genera such as Bacillus, Pseudomonas, Desulfotomaculum, Desulfovibrio, Desulfomonas, Methylococcus, Vibrio, Micrococcus, Klebsiella and Azotobacter. Principal component analysis (PCA) revealed a correlation among local environmental variables with the sampling locations on the microbial community in the mangrove soil. Rev. Biol. Trop. 60 (2): 909-924. Epub 2012 June 01.
Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products
Novel Research in Microbiology Journal, 2021
ISSN 2277-386X Original Article Microbial Biodiversity in Mangrove soils of Bhitarkanika, Odisha, India
Department of Biotechnology, College of Enginerring and Technology, Bhubaneswar751003, Odisha, In... more Department of Biotechnology, College of Enginerring and Technology, Bhubaneswar751003, Odisha, India 2Department of Biotechnology, North Orissa University, Baripada-757003, Odisha, India 3Microbiology Laboratory, Division of Crop Production, Central Rice Research Institute, Cuttack753006, Odisha, India 4 Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar-751024, Odisha, India *Correspondence: Fax: 0674-2386182, e-mail:hn_thatoi@rediffmail.com
Asian Journal of Biological and Life Sciences, 2020
In the present study, thirteen thermotolerant acetic acid bacteria were isolated from waste pomeg... more In the present study, thirteen thermotolerant acetic acid bacteria were isolated from waste pomegranate and apple fruit in Carr agar medium following the dilution plate technique method at 37°C. The bacterial strain capable to change the colour of the medium from green to yellow were considered as Acetobacter sp. Most of the bacterial isolate could able to tolerate 3-4% of ethanol concentration, change the colour and pH of the medium and produce acetic acid in the range of 1.1-15.2 g/L. Among thirteen bacterial strains, the bacterial strain PAAB-3 could able to tolerate maximum ethanol (5%) and produce maximum acetic acid (15.2g/L) at 37°C. On the basis of morphological and biochemical characteristics the bacterial isolate, PAAB-3 is tentatively identified as Acetobacter aceti.
Biotechnology Research and Innovation, 2017
Microorganisms are capable of converting insoluble phosphate into a bioavailable form through sol... more Microorganisms are capable of converting insoluble phosphate into a bioavailable form through solubilization and mineralization processes. Hence in the present study a phosphate solubilizing bacterium, PSB-26, was isolated from mangrove of the Mahanadi delta using NBRIP-agar and NBRIP-BPB broth containing tricalcium phosphate as the phosphate source. Based on phenotypic and molecular characterization, the strain was identified as Alcaligenes faecalis. The maximum phosphate solubilizing activity of the strain was found to be 48 g/ml with decrease in pH of the growth medium from 7.0 to 3.2. During phosphate solubilization, various organic acids, such as oxalic acid (289 mg/L), citric acid (0.2 mg/L), malic acid (0.3 mg/L), succinic acid (0.5 mg/L) and acetic acid (0.4 mg/L) produced in the broth culture were detected through HPLC analysis. Crude alkaline phosphatase activity of the strain was determined by p-nitrophenyl phosphate assay and optimized with different growth parameters to obtain maximum enzyme production. Under optimized sets of conditions, maximum alkaline phosphatase activity of 93.7 U/ml was observed. Partially purified alkaline phosphatase exhibited three protein bands of sizes approximately 45 kDa, 25 kDa and 17 kDa. Partially purified alkaline phosphatase during characterization showed maximum activity at pH 9.0 (96.53 U/ml), temperature of 45 • C (97.99 U/ml) and substrate concentration of 1.75 mg/ml (96.51 U/ml). The effect of the bacterium on growth of Arabidopsis thaliana plant showed that inoculation of bacterial culture exhibited better growth in comparison to the control. Hence the phosphate solubilizing and alkaline phosphatase production activity of the bacterium may have probable use for future biotechnological application.
Diversity and seasonal fluctuation of predominant microbial communities in Bhitarkanika, a tropical mangrove ecosystem in India
Revista de Biología Tropical, 2012
Biocatalysis and Biotransformation, 2016
The present study was conducted to isolate phosphate solubilising bacteria from mangrove soils of... more The present study was conducted to isolate phosphate solubilising bacteria from mangrove soils of Mahanadi river delta, Odisha, India and evaluate their phosphate solubilising ability. In total forty-eight phosphate solubilising bacteria were isolated from different soil samples. Based on their size of halo zone formation on NBRIP agar medium and decrease in intensity of colour of the broth medium fourteen isolates were selected as efficient phosphate solubilising strains. Their efficiency on NBRIP agar medium were ranged from 108-175. Their ability to decrease the intensity of bluecolour of the NBRIP-BPB broth medium was ranged from 0.87 and 1.188 (O.D at 600nm). Phosphate solubilising ability test of these fourteen isolates showed that they can solubilise tricalciumphosphate from 8.21 to 48.70μg/ml and most of the isolates could acidified the medium supernatant below 4.0 from the initial pH 7.0. Morphological and biochemical characterisation of the isolates allowed us to identify them as members of the following genera: Pseudomonas, Bacillus, Alcaligens, Klebsiella, Serratia, Azotobacters and Micrococcus. All the fourteen PSB isolated from mangrove soil of Mahanadi river delta could efficiently solubilise tricalcium phosphate in the medium which could probably help for future application in biotechnology.
Turkish Journal of Fisheries and Aquatic …, 2008
Mangrove ecosystem being suitable for brackish water shrimp culture, large number of aquaculture ... more Mangrove ecosystem being suitable for brackish water shrimp culture, large number of aquaculture ponds have been developed in the periphery of Bhitarkanika National Park area of Orissa, India. These ponds are regularly fed by brackish water from nearby Brahmani-Baitarani river system and the same water is ultimately discharged into the mangrove ecosystem. In the present study, some physico-chemical parameters such as pH, temperature, conductivity, dissolved oxygen, calcium, magnesium, total hardness, chlorine, phosphate, nitrate, and total dissolved solid contents of seven aquaculture ponds have been analysed for assessment of pollution status with respect to river water taken as control. Studies were undertaken for a period of six months from November 2005 to April 2006 during which the physico-chemical parameters were compared with the water quality standard of Bureau of Indian Standard (BIS) and Central and the State Pollution Control Board (PCB). pH values of the aquaculture ponds range between 5.63-8.5 as against the value of standard water quality i.e.6.5-8.5. Nitrate, total hardness, calcium, magnesium, phosphate, and chlorine contents show variation within different sites. Dissolved oxygen values vary from 3.0 to 8.3 mg/L during six months of investigation. Some of the sites show higher dissolved oxygen values than the permissible limits (4.0-5.0 mg/L) prescribed by PCB and BIS indicating that these waters do not pose any threat to aquatic life. The study indicates that the aquaculture pond water at present do not possess any pollution problem for the mangrove ecosystem.
African Journal of Biotechnology, 2009
In the present study both Gram positive and Gram negative bacteria were isolated using enrichment... more In the present study both Gram positive and Gram negative bacteria were isolated using enrichment media from five different stations from mangroves soils of Bhitarkanika, Orissa, India. Among the bacterial populations studied, the Gram negative bacterial population was found to be more in all the stations. Out of several Gram negative bacterial isolates, six predominant and morphologically distinct isolates were selected and characterized. Phenotypical the isolates were identified as one each of Pseudomonas aeruginosa and P. alcaligenes and two strains each of Methylococcus sp. and Desulfotomaculum sp. The strain P. aeruginosa and one strain of Methylococcus sp. tolerated up to 13% NaCl and 10% sea salt, respectively. But the other four isolates tolerated 10% NaCl as well as sea salt. Most of the organisms were sensitive to several antibiotics tested except for the antifungal antibiotic Nystatin. The stress tolerant enzyme activities for catalase, peroxidase, polyphenol oxidase, asc...
Reduction of selenite to red elemental selenium by moderately halotolerant Bacillus megaterium strains isolated from Bhitarkanika mangrove soil and characterization of reduced product
Chemosphere, 2011
Two Gram (+) bacterial strains, BSB6 and BSB12, showing resistance and potential for Se(IV) reduc... more Two Gram (+) bacterial strains, BSB6 and BSB12, showing resistance and potential for Se(IV) reduction among 26 moderately halotolerant isolates from the Bhitarkanika mangrove soil were characterized by biochemical and 16S rDNA sequence analyses. Both of them were strictly aerobic and able to grow in a wide range of pH (4-11), temperature (4-40°C) and salt concentration (4-12%) having an optimum growth at 37°C, pH ∼7.5 and 7% salt (NaCl). The biochemical characteristics and 16S rDNA sequence analysis of BSB6 and BSB12 showed the closest phylogenetic similarity with the species Bacillus megaterium. Both the strains effectively reduced Se(IV) and complete reduction of selenite (up to 0.25 mM) was achieved within 40 h. SEM with energy dispersive X-ray and TEM analyses revealed the formation of nano size spherical selenium particles in and around the bacterial cells which were also supported by the confocal micrograph study. The UV-Vis diffuse reflectance spectra and XRD of selenium precipitates revealed that the selenium particles are in the nanometric range and crystalline in nature. These bacterial strains may be exploited further for bioremediation process of Se(IV) at relatively high salt concentrations and green synthesis of selenium nanoparticles.
Universal Journal of Microbiology Research, 2016
As knowledge of sulphur oxidising bacterial communities in mangrove sediments is very sparse and ... more As knowledge of sulphur oxidising bacterial communities in mangrove sediments is very sparse and sulphur oxidizers have wide application such as in treatment of wastewater containing high sulphate levels which causes unwanted H 2 S production by sulfate reducing bacteria (SRB), oxidation of elemental sulphur to plant available sulphate for plant growth promotion, bio leaching, biocontroling agent etc. Hence, in the present study an attempt has been made to explore the diversity of sulphur oxidizing bacteria from mangroves of Mahanadi delta, Odisha to evaluate their biotechnological potential. Two sulphur oxidising bacteria (SOB-7and SOB-8) were isolated from mangrove soils of Mahanadi river delta, based on the change of colour of the thiosulphate broth medium from purple to colour less by reducing the pH. Based on morphological, biochemical and 16S rRNA gene sequencing the two strains (SOB-7) and (SOB-8) were identified as Klebsiella sp., and Micrococcus sp. respectively. The gene bank accession number of the strains are KR632644 (SOB-7) and KR632643 (SOB-8). The strain Klebsiella sp. and Micrococcus sp showed sulphate ion production ability of 243 mg/ml and 240 mg/ml respectively with decrease in pH from 7.0 to 4.0. Among these two isolates, SOB-8 showed higher sulphide oxidase production ability (126.83 U/ml) than the isolate, SOB-7 (126.0 U/ml). Maximum sulphide oxidase by both the strains were obtained at temperature 45°C, peptone as nitrogen source, thiosulphate concentration of 10 mg/ml. pH 9.0 for the isolate SOB-8 whereas pH 7.0 for the isolate, SOB-7. Partially purified enzyme of both the isolate showed higher activity at same substrate concentration of 1.5mg/ml, but varies with pH and temperature.
Microbial Biodiversity in Mangrove soils of Bhitarkanika, Odisha, India
Rev. Biol. Trop, Jan 1, 2012
Different groups of microorganisms are present in mangrove areas, and they perform complex intera... more Different groups of microorganisms are present in mangrove areas, and they perform complex interactions for nutrient and ecological balances. Since little is known about microbial populations in mangroves, this study analyzed the microbial community structure and function in relation to soil physico-chemical properties in Bhitarkanika, a tropical mangrove ecosystem in India. Spatial and seasonal fluctuations of thirteen important groups of microorganisms were evaluated from the mangrove forest sediments during different seasons, along with soil physico-chemical parameters. The overall microbial load (x10 5 cfu/g soil) in soil declined in the order of heterotrophic, free living N 2 fixing, Gram-negative nitrifying, sulphur oxidizing, Gram-positive, spore forming, denitrifying, anaerobic, phosphate solubilizing, cellulose degrading bacteria, fungi and actinomycetes. Populations of the heterotrophic, phosphate solubilizing, sulphur oxidizing bacteria and fungi were more represented in the rainy season, while, Gram-negative, Gram-positive, nitrifying, denitrifying, cellulose decomposing bacteria and actinomycetes in the winter season. The pool size of most of other microbes either declined or maintained throughout the season. Soil nutrients such as N, P, K (Kg/ha) and total C (%) contents were higher in the rainy season and they did not follow any common trend of changes throughout the study period. Soil pH and salinity (mS/cm) varied from 6-8 and 6.4-19.5, respectively, and they normally affected the microbial population dynamics. Determination of bacterial diversity in Bhitarkanika mangrove soil by culture method showed the predominance of bacterial genera such as Bacillus, Pseudomonas, Desulfotomaculum, Desulfovibrio, Desulfomonas, Methylococcus, Vibrio, Micrococcus, Klebsiella and Azotobacter. Principal component analysis (PCA) revealed a correlation among local environmental variables with the sampling locations on the microbial community in the mangrove soil. Rev. Biol. Trop. 60 (2): 909-924. Epub 2012 June 01.
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Papers by Rashmi Ranjan Mishra