Volumen 20 (Suplemento) by Marcos Meneses-Mayo
Objective. To characterize the fibrolytic enzymatic activity of Pleurotus ostreatus-IE8 and Fomes... more Objective. To characterize the fibrolytic enzymatic activity of Pleurotus ostreatus-IE8 and Fomes fomentarius-EUM1 in sugarcane bagasse (BCA); to evaluation of the kinetics of in vitro production of BCA treated by solid fermentation (FS), crude enzyme extract (ECE) of P. ostreatus-IE8 and Fibrozyme®. Materials and methods. In fungi measured radial growth rate ( Vcr ) and biomass production in two culture media (with or without nitrogen source); activity of xylanases, cellulases and FS on BCA at 0, 7 and 15 d. The chemical analysis and kinetic analysis of in vitro gas production in 4 treatments (ECE adding enzymes obtained from the direct addition FS or FS ), witness (Fibrozyme®) and a control without addition and analyzed by a was completely randomized design. Results. Xylanases (7 d ) showed 6.32 and 5.50 UI g-1 initial substrate dry weight (SSi) for fungi P. ostreatus-IE8 and F. fomentarius-EUM1 , respectively ; P. ostreatus-IE8 scored higher activity of laccases (10.65 g -1 UI SSi) and F. fomentarius-EUM1 (1.90 UI g-1 SSi) cellulases. The ECE of P. ostreatus-IE8 and commercial enzyme did not differences (p>0.05). In the chemical composition or the gas production kinetics. The 4 treatments evaluated decreased values of the variables measured in the kinetics of gas production compared to the control (p≤0.05). Conclusions. The ECE of P. ostreatus-IE8 was similar to commercial enzyme degradation in vitro, so it is feasible to use pre-digest high fiber products.
Papers by Marcos Meneses-Mayo
2Decanato de Ciencias Veterinarias
and incubation pH on in vitro degradation of NDF extracts of alfalfa and orchardgrass
The recurrent use of antibiotics has given the guideline so that bacteria will develop resistance... more The recurrent use of antibiotics has given the guideline so that bacteria will develop resistance to drugs used in medicine, which is why recent investigations have been directed to evaluate natural sources such as plants or fungi, which can fight the bacteria. Here the antibacterial activity of spent substrate of Pleurotus ostreatus combined with medicinal plants was evaluated. We designed six mixtures (barley straw, barley straw/Chenopodium ambrosioides L., barley straw/Mentha piperita L., barley straw/Rosmarinus officinalis L., barley straw/Litsea glaucescens Kunth and barley straw/Tagetes lucid Cav) to be used as a substrate of cultivation of mushroom. These were recovered after the harvest. We obtained aqueous extracts from spent substrates and resuspended them to different concentrations (25, 50 and 100 mg mL-1). These were tested for antibacterial activity against Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli; as a positive control we used azithromycin, c...
Agrociencia, 2010
Resumen es: La paja de sorgo, un esquilmo agricola abundante en Mexico y que se usa como alimento... more Resumen es: La paja de sorgo, un esquilmo agricola abundante en Mexico y que se usa como alimento para rumiantes, tiene baja digestibilidad por lo que se usan divers...
Actividad fibrolítica y producción de gas por Pleurotus ostreatus-IE8 y Fomes fomentarius-EUM1 en bagazo de caña
Revista Mvz Cordoba, 2015
Agrociencia, 2012
Las enzimas fibroliticas exogenas no son sintetizadas por los microorganismos ruminales, por lo c... more Las enzimas fibroliticas exogenas no son sintetizadas por los microorganismos ruminales, por lo cual se adicionan al alimento para aumentar la degradacion de la fibra. El objetivo de este estudio fue evaluar in vitro la actividad xilanasa, celulasa y lacasa, asi como la estabilidad enzimatica de xilanasas y celulasas de un producto enzimatico fibrolitico (Fibrozyme, Alltech, Inc.). El diseno experimental fue completamente al azar y los tratamientos fueron: amortiguador pH 6.0, amortiguador pH 7.0 y liquido ruminal clarificado pH 6.5. Los datos se analizaron con mediciones repetidas en el tiempo, mediante el procedimiento Mixto de SAS y la prueba de Tukey (p≤0.05). El producto enzimatico exogeno contenia xilanasas 292 UI g-1 y celulasas 36 UI g-1, sin actividad de lacasas. En amortiguador con pH 6 las celulasas fueron mas estables que las xilanasas, y ambas enzimas mostraron actividad hasta las 46 h. Las constantes de velocidad de inactivacion enzimatica (k) fueron —0.054 h-1 en xila...
Evaluación nutritiva y fermentativa del ensilado de dos subproductos agroindustriales brócoli ("Brassica oleracea, var italica") y alcachofa ("Cynara scolymus") para su empleo en alimentación animal / dirección María Dolores Megías Rivas y Antonio Martínez Teruel
Revista Mvz Cordoba, 2015
espanolObjetivos. Caracterizar la actividad enzimatica fibrolitica de Pleurotus ostreatus-IE8 y F... more espanolObjetivos. Caracterizar la actividad enzimatica fibrolitica de Pleurotus ostreatus-IE8 y Fomes fomentarius-EUM1 en bagazo de cana de azucar (BCA) y evaluar la cinetica de produccion de gas in vitro del BCA por fermentacion solida (FS) o con extractos crudos enzimaticos (ECE) de P. ostreatus-IE8 o Fibrozyme®. Materiales y metodos. En los hongos de estudio se evaluo la velocidad de crecimiento radial (Vcr) y produccion de biomasa en dos medios de cultivo (con o sin fuente de nitrogeno); actividad de xilanasas, celulasas y lacasas de la FS sobre BCA a 0, 7 y 15 d. El analisis quimico y cinetica de produccion de gas in vitro en 4 tratamientos (proceso de FS o adicion de enzimas obtenidas de ECE de la FS), un testigo (Fibrozyme®) y un control sin adicion de enzimas, todo ello se analizo en un diseno completamente al azar. Resultados. Las xilanasas (7 d) mostraron 6.32 y 5.50 UI g-1 sustrato seco inicial (SSi) en P. ostreatus-IE8 y F. fomentarius-EUM1, respectivamente. P. ostreatus...
Effect of a formulation with nopal (Opuntia ficus indica), amaranth (Amaranthus cruentus) and mushroom (Pleurotus ostreatus) in a murine model of diet-induced cardiometabolic disruptions
Cardiovascular diseases (CVD) are the first cause of death in Mexican population. Metabolic disru... more Cardiovascular diseases (CVD) are the first cause of death in Mexican population. Metabolic disruptions induced by diet are, in turn, associated with CVD. Within treatment, functional foods, such as nopal, amaranth and mushrooms, may be incorporated in order to attenuate such cardio metabolic diseases. The aim of this study was to develop nutritional bars based on three foods with beneficial potential to prevent cardio metabolic diseases due to the presence of important phytochemicals: nopal, amaranth and oyster mushroom. For this purpose, two experiments were carried out. First, four formulations for nutritional bars were elaborated based on the aforementioned foods. Proximate analysis, phenolic and flavonoid content were obtained for the four formulations; subsequently, the two that exhibited higher amounts of fiber, phenolic and flavonoid content were chosen for an in vivo experiment which aimed to evaluate the effect of those formulations on lipid profile, glycemia and body weig...
1Ganadería. Campus Montecillo. Colegio de Posgraduados. 56230. Montecillo, Estado de México (marc... more 1Ganadería. Campus Montecillo. Colegio de Posgraduados. 56230. Montecillo, Estado de México (marcela @colpos.mx). 2Zootecnia, Universidad Autónoma Chapingo. 56230, Chapingo, Estado de México. 3Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa. Avenida Sn Rafael Atlixco No. 186. Colonia Vicentina. 09430. Delegación Iztapalapa. 4Facultad de Ciencias de la Salud (Nutrición), Universidad Anáhuac, México-Norte. Avenida Universidad Anáhuac No. 46, Colonia Lomas Anáhuac, Huixquilucan, Estado de México 52786 (mmayo@colpos.mx)
Fibrolytic enzymes produced by solid-state fermentation to improve sugar cane silage
Resumen es: Los pastos y forrajes son la principal fuente de alimentos para los bovinos en el tro... more Resumen es: Los pastos y forrajes son la principal fuente de alimentos para los bovinos en el tropico, pero la produccion forrajera varia en calidad y cantidad a tra...
la actividad xilana-sa, celulasa y lacasa, asi como la estabilidad enzimatica de xilanasas y celu... more la actividad xilana-sa, celulasa y lacasa, asi como la estabilidad enzimatica de xilanasas y celulasas de un producto enzimatico fibrolitico (Fibrozyme, Alltech, Inc.). El diseno experimental fue com-pletamente al azar y los tratamientos fueron: amortiguador pH 6.0, amortiguador pH 7.0 y liquido ruminal clarificado pH 6.5. Los datos se analizaron con mediciones repetidas en el tiempo, mediante el procedimiento Mixto de SAS y la prueba de Tukey (p0.05). El producto enzimatico exoge
Oyster mushroom (Pleurotus ostreatus), nopal (Opuntia ficus-indica) and amaranth (Amaranthus spp.... more Oyster mushroom (Pleurotus ostreatus), nopal (Opuntia ficus-indica) and amaranth (Amaranthus spp.) are pre-hispanic foods widely consumed in Mexico. However, there are no standard products developed with these ingredients as functional cookies. This study evaluated the impact of partial replacement (50%) of whole-wheat flour (WWF) with three formulations of P. ostreatus, nopal and amaranth flours (POF, NF and AF, respectively) in the nutritional/antioxidant properties of fortified cookies. The proportion of flours´ ingredients (WWF:AF:NF:POF) were 100% WWF (traditional cookies), 50:35:10:5 (formulation 1), 50:30:15:5 (formulation 2) and 50:40:5:5 (formulation 3). Proximal composition, phenolic/flavonoid contents, and ABTS+ scavenging activity were determined in flours and cookies. POF, NF and AF possess a high nutritional value comprising polyphenols/flavonoids and a significant antioxidant potential. Total protein, ashes and flavonoids were higher in fortified cookies than controls...
Blood and Genomics
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease. Lupus nephritis (LN) affects... more Systemic lupus erythematosus (SLE) is a systemic autoimmune disease. Lupus nephritis (LN) affects 30% to 60% of adult Mexican patients. This study aimed to determine whether immunoglobulin free light chains (FLCs) constitute a biological marker for LN. FLCs have been previously studied as disease activity biomarkers, but patients with kidney damage are frequently excluded. A total of 75 consecutive SLE patients were recruited from a third-level hospital in Mexico City. Thirty-eight patients were diagnosed with LN, and the remaining 37 patients without kidney failure were included as a control group. FLCs concentration was measured by nephelometry. The results showed that SLE patients with high FLCs concentration had a higher rate of kidney failure and that the probability of renal failure increased by 2.4% for each mg/dL of κ FLCs, and 1.7% for each mg/dL of λ FLCs. This study suggests that high FLCs concentration is associated with LN, and therefore may be considered as a possible biomarker for SLE activity.
Prehispanic Functional Foods and Nutraceuticals in the Treatment of Dyslipidemia Associated to Cardiovascular Disease: a Mini-Review
International Journal for Vitamin and Nutrition Research, 2017
Dyslipidemia is an important modifi able risk factor for cardiovascular and metabolic diseases, w... more Dyslipidemia is an important modifi able risk factor for cardiovascular and metabolic diseases, which are responsible for a large number of mortality and disability cases around the globe. Different strategies have been used within the treatment of dyslipidemia, including lifestyle modifi cations, pharmacologic therapy, as well as functional foods and nutraceuticals. Functional foods have been used worldwide since ancient times, particularly, the prehispanic civilizations utilized several as medicinal foods. In the current pandemic of dyslipidemia as well as the nutritional transition, particularly in Latin America, the use of native functional foods represents an attractive target for the treatment and/ or prevention of these conditions. In this mini-review, evidence regarding different functional foods such as cacao, amaranth, chia, nopal, spirulina, as well as their nutraceutical compounds, including fl avonoids, omega-3 PUFAs, fi ber, prebiotics, lovastatin, c-phycocyanin, among...
Journal of Agricultural Science, 2015
The recurrent use of antibiotics has given the guideline so that bacteria will develop resistance... more The recurrent use of antibiotics has given the guideline so that bacteria will develop resistance to drugs used in medicine, which is why recent investigations have been directed to evaluate natural sources such as plants or fungi, which can fight the bacteria. Here the antibacterial activity of spent substrate of Pleurotus ostreatus combined with medicinal plants was evaluated. We designed six mixtures (barley straw, barley straw/Chenopodium ambrosioides L., barley straw/Mentha piperita L., barley straw/Rosmarinus officinalis L., barley straw/Litsea glaucescens Kunth and barley straw/Tagetes lucid Cav) to be used as a substrate of cultivation of mushroom. These were recovered after the harvest. We obtained aqueous extracts from spent substrates and resuspended them to different concentrations (25, 50 and 100 mg mL-1). These were tested for antibacterial activity against Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli; as a positive control we used azithromycin, cephalexin and dicloxacillin. The protocol was a completely randomized assay with a factorial arrangement design. The data were analyzed with PROC GLM, SAS. The spent substrate from Pleurotus ostreatus that contained Mentha piperita L. presented the largest zone of inhibition against Staphylococcus epidermidis (40.00 mm) which was similar to control antibiotics (40.00 mm). Second in toxicity was the spent substrate from barley straw extract (33.33 mm). In conclusion, the results suggest that it is possible to use the spent substrate of Pleurotus ostreatus as source of extracts with antibacterial activity, being the best option the combination of barley straw with Mentha piperita L.
Se ha realizado un estudio sobre la utilizacion de dos subproductos agroindustriales (brocoli esc... more Se ha realizado un estudio sobre la utilizacion de dos subproductos agroindustriales (brocoli escaldado y crudo y alcachofa cruda) mismos que tienen una mayor produccion por parte del sector industrial, asi como mayor demanda en el sector ganadero. El estudio comprende diferentes aspectos, dentro de los que destacan la conservacion de estos subproductos mediante el empleo de microsilos y silos-bolsa; en los que se ha estudiado el comportamiento fermentativo y la evaluacion quimico-nutritiva. Por otro lado, se ha evaluado la capacidad contaminante de los efluentes generados durante en ensilaje, asi como un estudio complementario sobre la presencia de fitosanitarios antes del ensilaje y despues de este. Tambien se ha realizado un estudio de digestibilidad in vivo en caprinos de la raza murciano-granadina, donde se han ofrecido dos dietas: una vase de ensilado de brocoli escaldadoy heno de alfalfa y otra con ensilado de alcachofa cruda. Adicionalmente se ha realizado un estudio de degr...
Bioresource Technology, 2008
Two strains of Pleurotus ostreatus (IE-8 and CP-50) were grown on defined medium added with wheat... more Two strains of Pleurotus ostreatus (IE-8 and CP-50) were grown on defined medium added with wheat straw extract (WSE). Mycelia from these cultures were used as an inoculum for solid fermentation using sugar cane bagasse (C:N=142). This substrate was used separately either as a mixture of heterogeneous particle sizes (average size 2.9mm) or as batches with two different particle sizes
Anti-inflammatory effect of aqueous extracts of spent Pleurotus ostreatus substrates in mouse ears treated with 12-O-tetradecanoylphorbol-13-acetate
Indian Journal of Pharmacology, 2016
To evaluate the application of spent Pleurotus ostreatus substrates, enriched or not with medicin... more To evaluate the application of spent Pleurotus ostreatus substrates, enriched or not with medicinal herbs, as a source of anti-inflammatory compounds. P. ostreatus was cultivated on five different substrates: Barley straw (BS) and BS combined 80:20 with medicinal herbs (Chenopodium ambrosioides L. [BS/CA], Rosmarinus officinalis L. [BS/RO], Litsea glaucescens Kunth [BS/LG], and Tagetes lucida Cav. [BS/TL]). The anti-inflammatory activity of aqueous extracts of spent mushroom substrates (SMSs) (4 mg/ear) was studied using an acute inflammation model in the mouse ear induced with 2.5 μg/ear 12-O-tetradecanoylphorbol13-acetate (TPA). Groups treated with BS/CA, BS/RO, and BS/LG aqueous extracts exhibited the best anti-inflammatory activity (94.0% ± 5.5%, 92.9% ± 0.6%, and 90.4% ± 5.0% inhibition of auricular edema [IAO], respectively), and these effects were significantly different (P < 0.05) from that of the positive control indomethacin (0.5 mg/ear). BS/TL and BS were also able to reduce TPA-induced inflammation but to a lesser extent (70.0% ± 6.7% and 43.5% ± 6.6% IAO, respectively). Spent P. ostreatus substrate of BS possesses a slight anti-inflammatory effect. The addition of CA L. to mushroom substrate showed a slightly synergistic effect while RO L. had an additive effect. In addition, LG Kunth and TL Cav. enhanced the anti-inflammatory effect of SMS. However, to determine whether there is a synergistic or additive effect, it is necessary to determine the anti-inflammatory effect of each medicinal herb.
Uploads
Volumen 20 (Suplemento) by Marcos Meneses-Mayo
Papers by Marcos Meneses-Mayo