The energetic parameters of Escherichia coli were analyzed for the aerobic/anaerobic transition. ... more The energetic parameters of Escherichia coli were analyzed for the aerobic/anaerobic transition. The electrochemical proton potential (Δ p) across the cytoplasmic membrane was determined in the steady state of respiration with O2, nitrate, fumarate, dimethylsulfoxide (Me2SO), and for fermentation. With O2, a proton potential of −160 mV was obtained. For anaerobic respiration with nitrate, fumarate or Me2SO, Δ p decreased only slightly by about 20 mV in contrast to earlier assumptions, whereas Δ p dropped by approximately 40 mV during fermentation. Under all conditions, the membrane potential (Δ @KY) contributed the major portion to Δ p. The cellular ATP levels were highest for aerobic growth (about 13 μmol/g dry cells) and decreased to 3−6 μmol/g in anaerobic metabolism. Δ .cf2.G.cf2..cf1..es.cf1.′.cf5..rb.eiPhos.rb, however, was constant due to equivalent changes of the ADP contents. Transition to the stationary growth phase caused a massive drop in the ATP content. It is concluded...
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