The ZLI is necessary and sufficient for the expression of thalamic differentiation genes in explant culture
<b>Copyright information:</b>Taken from "Tissue interactions in the developing c... more <b>Copyright information:</b>Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. Pro-Pth and pro-ZLI + pro-Pth explants were isolated from stage HH10 embryos, cultured for 72 hours and analysed for the expression of (red) and (blue). Pro-Th and pro-ZLI + pro-Th explants were isolated from stage HH10 embryos, cultured for 72 hours and analysed for the expression of (red) and (blue).
Co-culture of prechordal and epichordal neural explants results in ZLI induction
<b>Copyright information:</b>Taken from "Tissue interactions in the developing c... more <b>Copyright information:</b>Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. Explanted brain regions are schematically shown in a stage HH14 chick brain stained by hybridisation for the expression of (anterior to the left, dorsal to the top): 1, telencephalon; 2, pro-Pth; 3, pro-ZLI; 4, pro-Th; 5, midbrain; 6, hindbrain. Explants from brain regions as indicated in (A) were cultured in isolation (left two columns) or in combination (right column) for 24 hours and analysed for the expression of by hybridisation. Note induction of at the interface of co-cultured pro-Pth + pro-Th, pro-Pth + midbrain and telencephalon + pro-Th tissue, but not after co-culture of pro-Th + pro-Th or telencephalon + hindbrain. The yellow broken line in panel '2 + 4' indicates the interface between pro-Pth and pro-Th tissue. Explants from brain regions as indicated in (A) were cultured in isolation (left two columns) or in combination (right column) for 24 hours and analysed for the expression of (all in blue) and (red) by hybridisation. Note gap of expression in '2 + 4' explants (red arrow) and induction of in '1 + 5' explants (red arrowheads).
ZLI formation occurs independently from ventral tissues after stage HH10 and ZLI morphogenesis depends on the integrity of its flanking regions
<b>Copyright information:</b>Taken from "Tissue interactions in the developing c... more <b>Copyright information:</b>Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. Diencephalic explants were taken from stage HH10 and stage HH14 embryos and were analysed by hybridisation for the expression of (a-d, g-j) and (E, F) at the time of dissection (pre-cult.) or after culture for 48 (B, D, J) or 24 hours (F) (post-cult.). (A, B) Explants comprising pro-Pth, pro-ZLI, pro-Th and basal tissue (red). Note basal expression of at the time of dissection and formation of a ZLI after culture (red arrowhead). (C, D) Pro-Pth + pro-ZLI + pro-Th explants excluding basal tissue. Note absence of expression at the time of dissection and induction of a ZLI after culture. (E, F) Explants as in (C, D). Note formation of a -free stripe of cells after culture (red arrowhead). (G, H) Pro-Pth + pro-ZLI explants; (I, J) pro-ZLI + pro-Th explants. Note induction of in a patch, rather than a line, after culture (compare to (B, D)).
<b>Copyright information:</b>Taken from "Tissue interactions in the developing c... more <b>Copyright information:</b>Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. Whole embryos and explants were analysed by double hybridisation for expression of (red) and (blue). (A) Embryo at stage HH10, corresponding to the time when explants were dissected. Note expression of along the dorsal midline of the diencephalon and midbrain (black arrowheads). (B) Embryo at stage HH23, correponding to the developmental stage of explants after culture for 72 hours. Note expression of in a dorsal triangluar domain flanking the ZLI posteriorly (black arrowhead). (C) Explants excluding dorsal diencephalic tissue express only little at the time of explantation (pre-cult.) and form a ZLI after 72 hours in culture (D) (post-cult.). (E) Explants including dorsal diencephalic tissue express high levels of at the time of dissection and after culture but fail to express after culture (F).
Gene expression in the diencephalon during ZLI formation
Copyright information: Taken from "Tissue interactions in the developing chick diencephalon&... more Copyright information: Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. hybridisation was used to analyse the expression of (blue in (A, D); red in (C, E)), (blue in (B, E; red in (F)) and to stage HH14 (A, B), HH15 (C), HH18.5 (D) and HH22 (E, F) chick embryos. Whole heads are shown in (A-D) and hemisected brains in (E, F) (anterior to the left, dorsal to the top). Note expression of and in the ZLI (yellow arrowheads in (B, D-F)).
Peer support interventions for people living with HIV and AIDS (PLWHA) are widely effective, but ... more Peer support interventions for people living with HIV and AIDS (PLWHA) are widely effective, but their associated time and material costs for the recipient and the health system make them reachable for only a small proportion of PLWHA. Internet-based interventions are an effective alternative for delivering psychosocial interventions for PLWHA as they are more accessible and offer anonymity.Currently, no reviews are focusing on internet-based interventions with peer support components. This study aims to map the existing literature on psychosocial interventions for PLWHA based on peer support and delivered through the internet. We conducted a systematic scoping review of academic literature following PRISMA guidelines. Nine articles met our criteria, and we summarized them using a narrative synthesis method. We found internet-based peer support interventions for PLWHA are feasible and acceptable to participants and healthcare workers. We conclude that internet-based peer support int...
The impact that connected community have on precision health or medicine and vice versa offers op... more The impact that connected community have on precision health or medicine and vice versa offers opportunities for any type of research and survey information, e. g, to predict trends in health-related issues, specifically people behavior towards drug use. Here, precision medicine influences the way to treat the information and get a better outcome to support the stakeholder decision. Online social networks analysis seems to be good tools to quickly monitor the population behavior where users freely share large amounts of information related to their own lives on day-to-day basis. This novel kind of data can be used to get additional real time insights from people to understand their actual behavior related to drug use . The aim of this research is to generated an information fusion model of marijuana use tendency confident enough to be employed by stakeholders. So, we will: (a) collect and process the data from Twitter; (b) design a set of algorithms to estimate the tendency of marijuana use in relation to age, localization and gender, moreover, used a set of processes and activities to verify if our model were performing as expected; and (c) fusion of the information in a model to fully characterize the marijuana use population comparable to the national marijuana consume survey for policy makers utilization to improve drug use prevention. First,we collect the data from Twitter accounts based in Chile using an algorithm for traversing graph data structures, we collected the data from Twitter accounts based in Chile. Then, we estimated marijuana user prevalence during a period from 2006 to 2018 and, within each of the years we predicted the prevalence of user population in relation with age (in range), the localization (regions) and gender. Finally, we built indicators to explore the similarity between data obtained through Twitter (our results) and the actual data collected by the National Service for the Prevention and Rehabilitation of Drug and Alcohol (SENDA) under the same variables analyze in their own survey. When we compare the results of the algorithms and methods developed by us with those provided by the SENDA, we observed that most of the indicators present similar trends, i.e., the variation of the prevalence by years in the age, location and gender, showed similar changes in both analyzes. Also, the algorithms effectiveness and capacity to predict variations of complex cases like marijuana use in Chilean population. This study is a key opportunity to obtain in a faster, low cost and continuous way information about marijuana use, also, is an excellent tool for marijuana surveillance to get information to support policy makers and stakeholder decisions.
Background The developing vertebrate brain is patterned first by global signalling gradients that... more Background The developing vertebrate brain is patterned first by global signalling gradients that define crude anteroposterior and dorsoventral coordinates, and subsequently by local signalling centres (organisers) that refine cell fate assignment within pre-patterned regions. The interface between the prethalamus and the thalamus, the zona limitans intrathalamica (ZLI), is one such local signalling centre that is essential for the establishment of these major diencephalic subdivisions by secreting the signalling factor Sonic hedgehog. Various models for ZLI formation have been proposed, but a thorough understanding of how this important local organiser is established is lacking. Results Here, we describe tissue explant experiments in chick embryos aimed at characterising the roles of different forebrain areas in ZLI formation. We found that: the ZLI becomes specified unexpectedly early; flanking regions are required for its characteristic morphogenesis; ZLI induction can occur inde...
Is expressed in the dorsal diencephalon and is able to suppress ZLI formation
<b>Copyright information:</b>Taken from "Tissue interactions in the developing c... more <b>Copyright information:</b>Taken from "Tissue interactions in the developing chick diencephalon"http://www.neuraldevelopment.com/content/2/1/25Neural Development 2007;2():25-25.Published online 13 Nov 2007PMCID:PMC2217525. Stage HH15 chick head, hybridisation for expression of . Note expression in the dorsoanterior part of the eye, anterior to the MHB and in the epithalamus (red arrowhead). Stage HH16 embryos were electroporated with CYP1B1-IRES-GFP, cultured to stage HH21/22 and analysed by hybridisation for expression of followed by immunochemical detection of green flourescent protein (GFP; lateral views of hemisected brains, anterior to the left, dorsal to the top). (B) Non-electroporated control side (co). (C, D) Electroporated side (ep); (D) an overlay with the anti-GFP fluorescent signal in green. Note reduced expression in the ZLI even in areas where only few cells are transfected (red arrowhead), suggesting a non-autonomous effect.
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Papers by Flavia Guinazu