Cheng Chen

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The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells

BioMed research international, 2015

Objective. To investigate the role and possible mechanism of α-Klotho in the calcification and th... more Objective. To investigate the role and possible mechanism of α-Klotho in the calcification and the osteogenic transition of cultured VSMCs. Methods. VSMCs were cultured in vitro and divided into 5 groups, each using a different medium: (1) control; (2) β-GP; (3) β-GP + Klotho; (4) β-GP + LiCl; (5) β-GP + Klotho + LiCl. Calcium deposits were visualized using Alizarin Red S staining. The calcium concentrations were determined by the o-cresolphthalein complexone method. BMP2, Runx2 and β-catenin levels were estimated by western blotting, and the level of α-SMA was determined by using immunofluorescence at day 12. Results. β-GP induced an increase in the expression of BMP2, Runx2, and β-catenin. The calcium content increased, and the expression of α-SMA decreased. Alizarin Red S staining was positive under the high phosphorus conditions. BMP2, Runx2, and β-catenin levels and the calcium content decreased when the cells were cultured with rmKlotho; however, the levels of each were upregu...

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency

Nature communications, 2015

Small RNA-induced gene silencing is essential for post-transcriptional regulation of gene express... more Small RNA-induced gene silencing is essential for post-transcriptional regulation of gene expression; however, it remains unclear how miRNA/siRNA efficiency is regulated. Here we show that TARBP2 is SUMOylated at K52, which can be enhanced by its phosphorylation. This modification can stabilize TARBP2 via repressing its K(48)-linked ubiquitination. We find that TARBP2 SUMOylation does not influence the overall production of mature miRNAs, but it regulates miRNA/siRNA efficiency. SUMOylated TARBP2 recruits Ago2 to constitute the RNA-induced silencing complex (RISC)-loading complex (RLC), and simultaneously promotes more pre-miRNAs to load into the RLC. Consequently, Ago2 is stabilized and miRNAs/siRNAs bound by TARBP2/Dicer is effectively transferred to Ago2. Thus, these processes lead to the formation of the effective RISC for RNA interference (RNAi). Collectively, our data suggest that SUMOylation of TARBP2 is required for regulating miRNA/siRNA efficiency, which is a general mecha...

Flexible Rotating Beam: Comparative Modelling Of Isotropic And Composite Material Including Geometric Non-linearity

Journal of Sound and Vibration, 1994

A comparative modelling of a flexible rotating beam has been considered in this study. The dynami... more A comparative modelling of a flexible rotating beam has been considered in this study. The dynamic model is based on a beam with either isotropic material or composite material. The composite beam is considered as orthotropic fiber-reinforced and symmetrically laminated. Both small linear as well as geometric non-linear deformations are studied. A continuous system is considered in the analytical approach.

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Roles of Arabidopsis PARC6 in coordination of the chloroplast division complex and negative regulation of FtsZ assembly

Plant physiology, Jan 2, 2015

Chloroplast division is driven by the simultaneous constriction of the inner FtsZ ring (Z ring) a... more Chloroplast division is driven by the simultaneous constriction of the inner FtsZ ring (Z ring) and outer DRP5B ring. The assembly and constriction of these rings in Arabidopsis are coordinated partly through the inner envelope membrane (IEM) protein ARC6. Previously, we showed that PARC6 (Paralog of ARC6), also in the IEM, negatively regulates FtsZ assembly and acts downstream of ARC6 to position the outer envelope membrane (OEM) protein PDV1, which functions together with its paralog PDV2 to recruit DRP5B from a cytosolic pool to the OEM. However, whether PARC6, like ARC6, also functions in coordination of the chloroplast division contractile complexes was unknown. Here, we report a detailed topological analysis of Arabidopsis PARC6, which shows that PARC6 has a single transmembrane domain and a topology resembling that of ARC6. The newly identified stromal region of PARC6 interacts not only with ARC3, a direct inhibitor of Z-ring assembly, but also with the Z-ring protein FtsZ2. ...

A Novel Programming Method for Multilevel RRAM Based on Pulse Width Modulation

ECS Transactions

ABSTRACT

High Latent TB Infection Rate and Associated Risk Factors in the Eastern China of Low TB Incidence

PloS one, 2015

To disclose the associated risk factors for latent tuberculosis infection (LTBI) and the current ... more To disclose the associated risk factors for latent tuberculosis infection (LTBI) and the current situation of LTBI in the eastern China. A cross-sectional study was undertaken to evaluate the LTBI rate and risk factors. A total of 5305 subjects were finally included, with the IGRA positive rate of 19.98% (1060/5305). The LTBI rates were increasing with age (ORs were in significance from 6.60 to 20.92). Male gender significantly increased the risk of LTBI by 0.52 fold (OR = 1.52). Both smoking and drinking significantly increased the risk of LTBI (OR = 1.83 and OR = 1.67, respectively). Meanwhile, overweight and close contact with tuberculosis were risk factors for LTBI (OR = 1.36 and OR = 2.38, respectively). However, higher level of education and BCG vaccination lowered the risk of LTBI (OR = 0.16 and OR = 0.39, respectively). The multivariate logistic regression showed that age, male gender, smoking, overweight and close contacting with tuberculosis were risk factors for LTBI, but...

An Unconditionally Stable Explicit Integration Algorithm with Controllable Numerical Damping for Real-Time Testing

Structures Congress 2010, 2010

Minimizing Actuator Tracking and Energy Errors for Real-Time Hybrid Simulation through a New Adaptive Compensation Scheme

20th Analysis and Computation Specialty Conference, 2012

Real-Time Multi-Directional Hybrid Simulation of Building Piping Systems

Observations during past earthquakes have demonstrated the seismic vulnerability of nonstructural... more Observations during past earthquakes have demonstrated the seismic vulnerability of nonstructural components. Damage to these components can significantly reduce the functionality of essential facilities. Real-time hybrid simulation combines experimental testing and numerical simulation, and therefore provides an excellent technique for the dynamic testing of complete systems rather than testing components or subsystems. In this paper, a real-time multi-directional hybrid simulation of a system of nonstructural components is presented. In the simulation, a building piping system in a three-story moment resistant frame is subjected to bi-directional earthquake ground motions. The pressurized piping on the third story is selected as the experimental substructure, while the rest of the structure is modeled analytically. The Lehigh University Real-Time Multi-Directional Seismic Simulation Facility is used for the study. To ensure accuracy and stability during the simulation, the newly developed unconditionally stable explicit CR integration algorithm and inverse compensation method for actuator delay are used. The two horizontal components of the 1994 Northridge earthquake ground motion recorded at Canoga Park are scaled to the MCE seismic hazard level. Real-time hybrid simulation is performed to evaluate the seismic performance of the components of the piping system, including the bracing, joints, and piping members. The simulation results indicate that adequate piping joints and carefully designed bracing can enable the nonstructural piping system to perform well under strong earthquakes. The experimental study presented in this paper demonstrates the application of real-time hybrid simulation to the seismic testing of nonstructural components.

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Professional Development for Community College Students through Design and Seismic Evaluation of Three-Story Moment Resisting Frame

Earthquake has posed a great danger for the human society especially in the San Francisco Bay Are... more Earthquake has posed a great danger for the human society especially in the San Francisco Bay Area. Future earthquake disaster prevention and preparation require that young professional civil engineers are trained and recruited into the next generation workforce for the purpose of public safety. For the 2011 NASA CIPAIR (Curriculum Improvements and Partnership Award for the Integration of Research) internship program, four community college students from Cañada College participated in a ten-week study of earthquake engineering design and analysis. Students conducted research on seismic systems, structural design, and time history analysis. Specifically, a three-story steel special moment resisting frame was designed and evaluated under selected ground motions. During the structural design, the students not only utilized building codes to determine appropriate sizes for beam and column members of the building but also conducted research on dynamic time history analysis. Four sets of recorded earthquake ground motion -Landers, Loma Prieta, Kobe, and Northridge Earthquakes -were used to assess the performance of the design steel structure. Structural analysis program SAP2000 was incorporated into the design process for students to examine story drift, and the bending of the structure's members. In addition to learning about the fundamentals of earthquake engineering analysis and design, the interns also delivered an interactive presentation to local high school students to encourage them to pursue careers in math, science and engineering. Lastly, the interns created tutorials and videos to help improve community college and university curriculum. Internship programs such as the NASA CIPAIR help prepare community college students not only for their chosen university, but also for their professional career.

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Cadmium exposure and risk of pancreatic cancer: a meta-analysis of prospective cohort studies and case-control studies among individuals without occupational exposure history

Environmental science and pollution research international, 2015

Pancreatic cancer is one of the most deadly types of cancer for both genders. Classified as a hum... more Pancreatic cancer is one of the most deadly types of cancer for both genders. Classified as a human carcinogen, cadmium has been related to diverse cancers. However, the association between cadmium exposure and the risk of pancreatic cancer is still unclear. We quantitatively reviewed the observational studies on the association of cadmium exposure with pancreatic cancer risk among individuals without occupational exposure history published through July 2014 in PubMed by using a fixed-effect model. Four prospective cohort studies (112,934 participants with 335 events) and two case-control studies (177 cases and 539 controls) were identified. The summarized relative risk (RR) with a 95 % confidence interval (CI) was 2.05 (95 % CI = 1.58-2.66), comparing the highest to the lowest category of cadmium exposure. This positive association persisted in men (RR = 1.78, 95 % CI = 1.04-3.05) but not in women (RR = 1.02, 95 % CI = 0.63-1.65). Further research is needed to provide more solid ev...

Analysis of decimation techniques to improve computational efficiency of a frequency-domain evaluation approach for real-time hybrid simulation

SMART STRUCTURES AND SYSTEMS

Accurate actuator tracking is critical to achieve reliable real-time hybrid simulation results fo... more Accurate actuator tracking is critical to achieve reliable real-time hybrid simulation results for earthquake engineering research. The frequency-domain evaluation approach provides an innovative way for more quantitative post-simulation evaluation of actuator tracking errors compared with existing time domain based techniques. Utilizing the Fast Fourier Transfonn the approach analyzes the actuator en-or in terms of amplitude and phrase errors. Existing application of the approach requires using the complete length of the experimental data. To improve the computational efficiency, two techniques including data decimation and frequency decimation are analyzed to reduce the amount of data involved in the frequency-domain evaluation. The presented study aims to enhance the computational efficiency of the approach in order to utilize it for future on-line actuator tracking evaluation. Both computational simulation and laboratory experimental results are analyzed and recommendations on t...

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Solvent-Free Synthesis of Crystalline Metal Phosphate Oxalates with a (4,6)-Connected fsh Topology

Inorganic chemistry, Jan 16, 2015

Two new organically templated manganese phosphate oxalates were synthesized under solvent-free co... more Two new organically templated manganese phosphate oxalates were synthesized under solvent-free conditions. They have different three-dimensional structures with a (4,6)-connected fsh topology. It is interesting that the amine molecules can reverse the structure-building roles of the metal phosphate skeleton and metal oxalate substructure for formation of their fsh-type frameworks.

e-service robot in home healthcare

Proceedings. 2000 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2000) (Cat. No.00CH37113), 2000

... We apply color cues in this system in two ways. First one is the background of the navigation... more

Plasma retinol and α-tocopherol status of the Taiwanese elderly population

Biochemical assessment of vitamin A and vitamin E status of Taiwanese elderly persons was conduct... more Biochemical assessment of vitamin A and vitamin E status of Taiwanese elderly persons was conducted by quantitative analysis of the concentration of retinol and α-tocopherol in plasma samples collected in the Elderly Nutrition and Health Survey in Taiwan (1999-2000). Plasma samples were analyzed by a reverse phase HPLC that can detect retinol and α-tocopherol simultaneously. The mean (SE) plasma retinol

WSS25, a sulfated polysaccharide, inhibits RANKL-induced mouse osteoclast formation by blocking SMAD/ID1 signaling

Acta pharmacologica Sinica, Jan 24, 2015

WSS25 is a sulfated polysaccharide extracted from the rhizome of Gastrodia elata BI, which has be... more WSS25 is a sulfated polysaccharide extracted from the rhizome of Gastrodia elata BI, which has been found to bind to bone morphogenetic protein 2 (BMP-2) in hepatocellular cancer cells. Since BMP-2 may regulate both osteoclasts and osteoblasts, here we investigated the effects of WSS25 on osteoclastogenesis in vitro and bone loss in ovariectomized mice. RAW264.7 cells or mouse bone marrow macrophages (BMMs) were treated with RANKL to induce osteoclastogenesis, which was assessed using TRAP staining, actin ring formation and pit formation assays, as well as bone resorption assay. Cell viability was detected with MTT assay. The mRNA levels of osteoclastogenesis-related genetic markers (TRAP, NFATc1, MMP-9 and cathepsin K) were detected using RT-PCR, while the protein levels of p-Smad1/5/8 and Id1 were measure with Western blotting. WSS25 was administered to ovariectomized mice (100 mg·kg(-1)·d(-1), po) for 3 months. After the mice were euthanized, total bone mineral density and cortic...

A loop partition technique for reducing cache bank conflict in multithreaded architecture

IEE Proceedings - Computers and Digital Techniques, 1996

Parallel multithreaded architectures take advantage of the ability to execute more than one threa... more Parallel multithreaded architectures take advantage of the ability to execute more than one thread simultaneously on a single chip at low synchronisation and communication costs and high hardware resource utilisation. However, a high bandwidth cache, such as a multibank cache, is especially critical to serve memory accesses issued at the same time from different threads. To prevent bank conflicts of multibank cache from seriously degrading system performance, a loop partition method is proposed to reduce or even eliminate bank conflicts. The partition allows each thread access to certain bank modules and prevents any two from accessing the same bank module. The method neither slows down the clock rate nor increases the array subscript expression complexity. The performance gains of the bank-conflict-free loop partition approach are shown in simulation results.

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Fluoxetine ameliorates cognitive impairments induced by chronic cerebral hypoperfusion via down-regulation of HCN2 surface expression in the hippocampal CA1 area in rats

Pharmacology, biochemistry, and behavior, Jan 5, 2015

Chronic cerebral hypoperfusion (CCH) causes cognitive impairments and increases the risk of Alzhe... more Chronic cerebral hypoperfusion (CCH) causes cognitive impairments and increases the risk of Alzheimer disease (AD) and vascular dementia (VD) through several biologically plausible pathways, yet the underlying neurobiological mechanisms is still poorly understood. In this study, we investigated whether fluoxetine, a selective serotonin reuptake inhibitor (SSRI), could play neuroprotective role against chronic cerebral hypoperfusion injury and to clarify underlying mechanisms of its efficacy. Rats were subjected to permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO). Two weeks later, rats were treated with 30mg/kg fluoxetine (intragastric injection, i.g.) for 6weeks. Cognitive function was evaluated by Morris water maze (MWM) and novel objects recognition (NOR) test. Long-term potentiation (LTP) was used to address the underlying synaptic mechanisms. Western blotting was used to quantify the protein levels. Our results showed that fluoxetine treat...

Dynamic Environment Modeling with Gridmap: A Multiple-Object Tracking Application

2006 9th International Conference on Control, Automation, Robotics and Vision, 2006

The Bayesian occupancy filter (BOF) (1) has achieved promising results in the object tracking app... more The Bayesian occupancy filter (BOF) (1) has achieved promising results in the object tracking applications. This paper presents a new development of BOF which inherits original BOF&#39;s advantages in handling occlusion and representing objects&#39; shape. Meanwhile, the new formulation has significantly reduced original BOF&#39;s complexities and can be run in realtime. In Bayesian occupancy filter, the environment is finely divided

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Grey Markov Model Forecast in Economic System under Incomplete Information and its Application on Foreign Direct Investment

2011 International Conference on Information Management, Innovation Management and Industrial Engineering, 2011

Foreign direct investment (FDI) plays an extraordinary role in developing countries and its fluct... more Foreign direct investment (FDI) plays an extraordinary role in developing countries and its fluctuations reflect the changes of influencing factors during time, and therefore the models to simulate and forecast the trends are of great significance. Model GM (1, 1) is used in this paper to overcome the problems of small sample size and poor data. In the pre-procession of

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