The Humanities and Indigenous Knowledge by Amelia Hilgart
In Paulshoek, Namaqualand, three research projects focusing on medicinal plants were developed co... more In Paulshoek, Namaqualand, three research projects focusing on medicinal plants were developed concurrently. The projects were based in the disciplines of anthropology, botany and chemistry. In this paper, we explore how these projects related to one another and describe the conversations that occurred in the process of searching for transdisciplinary knowledge. The projects ostensibly shared a common object of knowledge, but it was through working together that the medicinal plants constituted us as a community of scholars. As our insight into our respective disciplinary relationships with the plants grew, so did our understanding of the limitations of our respective disciplinary positions. The process made possible a ‘reimagination’ of both the object of study and our relationships to it and to one another. The research project, conceptualised in 2009, engaged current debates on indigenous knowledge and its historical erasures, and offered an approach that has potential to produce new knowledges while respecting the integrity of the disciplines. This approach requires a non-competitive attitude to research and one that acknowledges the contributions that can be made by multiple approaches.
Papers by Amelia Hilgart
Plants, People and Health: Three Disciplines at work in Namaqualand, by Lesley Green, David Gammon, Timm Hoffman, Robert Morrell, Helen Verran, Josh Cohen, Nicola Wheat, Amelia Hilgart
In Paulshoek, Namaqualand, three research projects focussing on medicinal plants were developed c... more In Paulshoek, Namaqualand, three research projects focussing on medicinal plants were developed concurrently. The projects were based in the disciplines of anthropology, botany and chemistry. In this paper we explore how they related to one another and describe the conversations that occurred in the process of searching for transdisciplinary knowledge. The projects ostensibly shared a common object of knowledge but it was through working together that the medicinal plants constituted us as a community of scholars. As our insight into our respective disciplinary relationships with the plants grew, so did our understanding of the limitations of our respective disciplinary positions. The process made possible a reimagination of both the object of study and our relationships to it and to one another. The research project, conceptualised in 2009, engages current debates on indigenous knowledge and its historical erasures, and offers an approach that has potential to produce new knowledge...
A molecular physiological review of vegetative desiccation tolerance in the resurrection plant Xerophyta viscosa (Baker)
Planta, 2015
Provides a first comprehensive review of integrated physiological and molecular aspects of desicc... more Provides a first comprehensive review of integrated physiological and molecular aspects of desiccation tolerance Xerophyta viscosa . A synopsis of biotechnological studies being undertaken to improve drought tolerance in maize is given. Xerophyta viscosa (Baker) is a monocotyledonous resurrection plant from the family Vellociacea that occurs in summer-rainfall areas of South Africa, Lesotho and Swaziland. It inhabits rocky terrain in exposed grasslands and frequently experiences periods of water deficit. Being a resurrection plant it tolerates the loss of 95 % of total cellular water, regaining full metabolic competency within 3 days of rehydration. In this paper, we review some of the molecular and physiological adaptations that occur during various stages of dehydration of X. viscosa, these being functionally grouped into early and late responses, which might be relevant to the attainment of desiccation tolerance. During early drying (to 55 % RWC) photosynthesis is shut down, there is increased presence and activity of housekeeping antioxidants and a redirection of metabolism to the increased formation of sucrose and raffinose family oligosaccharides. Other metabolic shifts suggest water replacement in vacuoles proposed to facilitate mechanical stabilization. Some regulatory processes observed include increased presence of a linker histone H1 variant, a Type 2C protein phosphatase, a calmodulin- and an ERD15-like protein. During the late stages of drying (to 10 % RWC) there was increased expression of several proteins involved in signal transduction, and retroelements speculated to be instrumental in gene silencing. There was induction of antioxidants not typically found in desiccation-sensitive systems, classical stress-associated proteins (HSP and LEAs), proteins involved in structural stabilization and those associated with changes in various metabolite pools during drying. Metabolites accumulated in this stage are proposed, inter alia, to facilitate subcellular stabilization by vitrification process which can include glass- and ionic liquid formation.
Main conclusion Provides a first comprehensive review of integrated physiological and molecular a... more Main conclusion Provides a first comprehensive review of integrated physiological and molecular aspects of desiccation tolerance Xerophyta viscosa. A synopsis of biotechnological studies being undertaken to improve drought tolerance in maize is given. Xerophyta viscosa (Baker) is a monocotyledonous resurrection plant from the family Vellociacea that occurs in summer-rainfall areas of South Africa, Lesotho and Swaziland. It inhabits rocky terrain in exposed grasslands and frequently experiences periods of water deficit. Being a resurrection plant it tolerates the loss of 95 % of total cellular water, regaining full metabolic competency within 3 days of rehydration. In this paper, we review some of the molecular and physiological adaptations that occur during various stages of dehydration of X. viscosa, these being functionally grouped into early and late responses, which might be relevant to the attainment of desiccation tolerance. During early drying (to 55 % RWC) photosynthesis is shut down, there is increased presence and activity of housekeeping antioxidants and a redirection of metabolism to the increased formation of sucrose and raffinose family oligosaccharides. Other metabolic shifts suggest water replacement in vacuoles proposed to facilitate mechanical stabilization. Some regulatory processes observed include increased presence of a linker histone H1 variant, a Type 2C protein phosphatase, a calmodulin-and an ERD15-like protein. During the late stages of drying (to 10 % RWC) there was increased expression of several proteins involved in signal transduction, and retroelements speculated to be instrumental in gene silencing. There was induction of antioxidants not typically found in desiccation-sensitive systems, classical stress-associated proteins (HSP and LEAs), proteins involved in structural stabilization and those associated with changes in various metabolite pools during drying. Metabolites accumulated in this stage are proposed, inter alia, to facilitate subcellular stabilization by vitrification process which can include glass-and ionic liquid formation. Keywords Physiology Á Proteome Á Resurrection plant Á Transcriptome Á Vegetative desiccation tolerance Abbreviation ERD (LRD) Early (Late) response to desiccation
ISRN Obstetrics and Gynecology, 2011
Maternal vitamin B12 deficiency during pregnancy is an independent risk factor for neural tube de... more Maternal vitamin B12 deficiency during pregnancy is an independent risk factor for neural tube defects and other neurological problems in infants. We determined the vitamin B12 status of 143 pregnant women in Nigeria representing all trimesters who presented to an antenatal clinic in Jos, Nigeria, using holotranscobalamin II levels (holoTCII), which is a measure of the vitamin B12 that is available for uptake into tissues. The holoTCII concentration ranged from 13 to 128 pmol/L. Using a cutoff of 40 pmol/L, 36% of the women were classified as vitamin B12-deficient. HoloTCII concentrations correlated negatively with plasma homocysteine levels (r = −0.24, P = 0.003) and positively with red blood cell folate concentrations (r = 0.28, P < 0.001). These data underscore the importance of supplementing pregnant women in Nigeria with vitamin B12 in order to ensure adequate vitamin B12 status and decrease the risk for neural tube defects.
A molecular physiological review of vegetative desiccation tolerance in the resurrection plant Xerophyta viscosa (Baker)
Planta, 2015
Provides a first comprehensive review of integrated physiological and molecular aspects of desicc... more Provides a first comprehensive review of integrated physiological and molecular aspects of desiccation tolerance Xerophyta viscosa . A synopsis of biotechnological studies being undertaken to improve drought tolerance in maize is given. Xerophyta viscosa (Baker) is a monocotyledonous resurrection plant from the family Vellociacea that occurs in summer-rainfall areas of South Africa, Lesotho and Swaziland. It inhabits rocky terrain in exposed grasslands and frequently experiences periods of water deficit. Being a resurrection plant it tolerates the loss of 95 % of total cellular water, regaining full metabolic competency within 3 days of rehydration. In this paper, we review some of the molecular and physiological adaptations that occur during various stages of dehydration of X. viscosa, these being functionally grouped into early and late responses, which might be relevant to the attainment of desiccation tolerance. During early drying (to 55 % RWC) photosynthesis is shut down, there is increased presence and activity of housekeeping antioxidants and a redirection of metabolism to the increased formation of sucrose and raffinose family oligosaccharides. Other metabolic shifts suggest water replacement in vacuoles proposed to facilitate mechanical stabilization. Some regulatory processes observed include increased presence of a linker histone H1 variant, a Type 2C protein phosphatase, a calmodulin- and an ERD15-like protein. During the late stages of drying (to 10 % RWC) there was increased expression of several proteins involved in signal transduction, and retroelements speculated to be instrumental in gene silencing. There was induction of antioxidants not typically found in desiccation-sensitive systems, classical stress-associated proteins (HSP and LEAs), proteins involved in structural stabilization and those associated with changes in various metabolite pools during drying. Metabolites accumulated in this stage are proposed, inter alia, to facilitate subcellular stabilization by vitrification process which can include glass- and ionic liquid formation.
English
South African Journal of Science, 2015
Plants, People and Health: Three Disciplines at work in Namaqualand, by Lesley Green, David Gammon, Timm Hoffman, Robert Morrell, Helen Verran, Josh Cohen, Nicola Wheat, Amelia Hilgart
In Paulshoek, Namaqualand, three research projects focussing on medicinal plants were developed c... more In Paulshoek, Namaqualand, three research projects focussing on medicinal plants were developed concurrently. The projects were based in the disciplines of anthropology, botany and chemistry. In this paper we explore how they related to one another and describe the conversations that occurred in the process of searching for transdisciplinary knowledge. The projects ostensibly shared a common object of knowledge but it was through working together that the medicinal plants constituted us as a community of scholars. As our insight into our respective disciplinary relationships with the plants grew, so did our understanding of the limitations of our respective disciplinary positions. The process made possible a reimagination of both the object of study and our relationships to it and to one another. The research project, conceptualised in 2009, engages current debates on indigenous knowledge and its historical erasures, and offers an approach that has potential to produce new knowledge...
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The Humanities and Indigenous Knowledge by Amelia Hilgart
Papers by Amelia Hilgart