Papers by Aleksandra Obrępalska-Stęplowska
Bulletin of Entomological Research, May 24, 2018
Diagnostyka molekularna polskich populacji niszczyka zjadliwego (Ditylenchus dipsaci)
Progress in Plant Protection, 2010
Wybrane mechanizmy nabywania odpornosci organizmow na srodki ochrony roslin
Postepy Biologii Komorki, 2006
bioRxiv (Cold Spring Harbor Laboratory), Jul 8, 2022
Deciphering of BTH-induced response of tomato (Solanum lycopersicum L.) and its effect on plant v... more Deciphering of BTH-induced response of tomato (Solanum lycopersicum L.) and its effect on plant virus infection through the multi-omics approach Frąckowiak Patryk https://orcid.
Molecular diagnostic of Polish population of Ditylenchus dipsaci
Progress in Plant Protection, 2010
Clinical significance of the insulin-like growth factor I gene promoter (P1) polymorphism in thyroid nodular disease
Neuro endocrinology letters, Dec 1, 2005
Objectives: Due to the recent increase of incidence of thyroid nodules and the known risk of mali... more Objectives: Due to the recent increase of incidence of thyroid nodules and the known risk of malignant transformation, there is an elevated risk of thyroid cancer in Poland. Several approaches, including molecular, have been proposed to support fine needle aspiration biopsy in the early detection of malignant lesions. Although the IGF-I system in thyroid cancer has been studied, little is known about the gene and its promoter structure changes. Our aim was to assess, whether the analysis of the IGF-I gene promoter region and 5'UTR exon 1 structure may be useful in assessing the risk of thyroid carcinoma. Material: Our study included 46 patients that underwent strumectomy due to a presence of thyroid nodules. Methods: All patients underwent clinical examination and laboratory investigations to assess their thyroid structure and function. Tissues obtained during the surgery were used for DNA extraction, PCR, SSCP and direct sequencing. Results: Among 46 patients, 14 had a nucleotide difference in one of the examined regions. In our study we revealed no significant difference between carcinomatous and non-carcinomatous groups of patients in terms of presence of nucleotide change, but Fisher's exact test gave a significant result in terms of the efficacy of detecting follicular adenoma. Moreover, the patients with nucleotide change had thyroid glands significantly smaller in volume. Conclusions: We conclude that the molecular analysis of the IGF-I gene promoter is thought to be of a functional significance, but probably could not be considered useful in the assessment of risk of thyroid cancer in thyroid nodules.
Biotechnologia, 2008
Microorganisms in biological control of plant-parasitic nematodes S u m m a r y Nematodes are uns... more Microorganisms in biological control of plant-parasitic nematodes S u m m a r y Nematodes are unsegmented roundworms that numerously and successfully adapted to all regions and environments on earth. The last ones were usually classified into feeding types: free-living, predaceous, and parasitic-including plant-parasitic. They are of great significance in terms of damage they cause. Plant-parasitic nematodes have been reported to be responsible for the losses amounting to over $100 billion throughout the world. Because of the big difficulties in their eradication some of them are considered as quarantine species. The plant-parasitic nematodes are controlled using chemical methodsmainly chemical nematicides. However, because of many drawbacks including health and environmental concerns, other control methods are considered. One of them is biological control and application of antagonistic microorganisms to decrease densities of nematodes populations. Microbial antagonists parasitizing various developmental stages of their hosts may affect nematodes by secretion of antibiotics, toxins and other secondary metabolites. The most important virulence factors are extracellular enzymes that participate in destroying the nematodes' cuticle or the eggshell or in further phases of infection. This publication presents the examples of microorganisms investigated in terms of biological control, those that are already available commercially as well as some mechanisms involved in nematode-microbes interactions.
Progress in Plant Protection, 2012
In June 2010, the presence of 40 cysts of nematodes from the Globodera genus was revealed in a so... more In June 2010, the presence of 40 cysts of nematodes from the Globodera genus was revealed in a soil sample collected from the field in the area of Opolskie Voivodeship in Poland. The results of the morphological and morphometric analyses revealed the presence of 30 cysts with features characteristic for the species Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959, and 10 cysts with features characteristic for the species G. pallida (Stone, 1973) Behrens, 1975. The results of molecular tests (PCR-Polymerase Chain Reaction and PCR-RFLP-Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) confirmed the presence of both nematode species in the sample. Sequence of rDNA fragment (ribosomal deoxyribonucleic acid) obtained in the PCR amplification and comparative analysis of the sequence revealed approximately 97% identity with a sequence characteristic for G. pallida deposited in the NCBI database (National Center for Biotechnology Information), which confirmed the presence of this species in the sample.
Analysis of the Human Growth Hormone Receptor and IGF-I Coding Sequences in Children with Growth Disorders
Journal of Pediatric Endocrinology and Metabolism, 2003
Analysis of GHR and IGF-I coding sequences in 47 children with normal serum levels of GH, low IGF... more Analysis of GHR and IGF-I coding sequences in 47 children with normal serum levels of GH, low IGF-I and growth disorders generally did not show mutation in the genes studied. Only one boy had a mutation located in the fifth exon of the GHR gene (C-->T in codon 88). This suggests that the growth disorders in this group of children might be due to a defect in a DNA region regulating expression of the GHR and IGF1 genes or genes involved in their regulation.
Archives of Virology, Feb 18, 2016
Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in ... more Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.
Analysis of Coding and Promoter Sequences of the IGF-I Gene in Children with Growth Disorders Presenting with Normal Level of Growth Hormone
Journal of Pediatric Endocrinology and Metabolism, 2003
The insulin-like growth factor-I (IGF-I) gene was analyzed in a population of children with growt... more The insulin-like growth factor-I (IGF-I) gene was analyzed in a population of children with growth disorders presenting normal GH and low IGF-I. We thus tried to detect any mutation in the IGF-I gene that could be responsible for short stature in children, using PCR, single-strand conformation polymorphism (SSCP) analysis, followed by DNA cloning and sequencing. We demonstrated in all examined children significant changes in the promoter region of the IGF-I gene (P1 IGF-I). Nucleotide sequence changes, such as CC-->GT and A-->G, and their localization are described. The results obtained excluded mutations in the coding sequence of the IGF-I gene. We conclude that testing the IGF-I P1 region, using PCR/SSCP analysis, could be useful in the diagnosis of growth disorders.
Parasitology Research, May 16, 2008
Cyst nematodes from the genus Globodera are common, and widely distributed parasites of Solaracea... more Cyst nematodes from the genus Globodera are common, and widely distributed parasites of Solaraceae. Intact cysts persist in soil even up to 10 years without detriment. Out of two Globodera species occurring in Poland, Globodera rostochiensis is considered by European and Mediterranean Plant Protection Organization (EPPO) as a quarantine pest, while Globodera artemisiae is not. Therefore, the distinction between these two species is crucial. Classic methods of detection and differentiation are laborious and time-consuming. Instead, application of molecular biology techniques allows obtaining of rapid and reliable results. The aim of this study was to establish detection and differentiation method of two species, G. rostochiensis and G. artemisiae, based upon real-time polymerase chain reaction with the use of TaqMan probes. In reaction with primers and probes specific for the nematodes' ribosomal DNA (rDNA), the samples used were DNAs isolated from the two species, alone or in mixture, as well as crushed single cysts. Applied probes enable not only to identify the species in DNA mixtures but also in a single cyst. The use of a crushed cyst eliminates long-lasting procedure of DNA isolation and reduces costs of analysis.
Scientific Reports, Jul 17, 2023
To ensure sufficient food supply worldwide, plants are treated with pesticides to provide protect... more To ensure sufficient food supply worldwide, plants are treated with pesticides to provide protection against pathogens and pests. Herbicides are the most commonly utilised pesticides, used to reduce the growth of weeds. However, their long-term use has resulted in the emergence of herbicideresistant biotypes in many weed species. Cornflower (Centaurea cyanus L., Asteraceae) is one of these plants, whose biotypes resistant to herbicides from the group of acetolactate synthase (ALS) inhibitors have begun to emerge in recent years. Some plants, although undesirable in crops and considered as weeds, are of great importance in phytomedicine and food production, and characterised by a high content of health-promoting substances, including antioxidants. Our study aimed to investigate how the acquisition of herbicide resistance affects the health-promoting properties of plants on the example of cornflower, as well as how they are affected by herbicide treatment. To this end, we analysed non-anthocyanin polyphenols and antioxidant capacity in flowers of C. cyanus from herbicide-resistant and susceptible biotypes. Our results indicated significant compositional changes associated with an increase in the content of substances and activities that have health-promoting properties. High antioxidant activity and higher total phenolic and flavonoid compounds as well as reducing power were observed in resistant biotypes. The latter one increased additionally after herbicide treatment which might also suggest their role in the resistance acquisition mechanism. Overall, these results show that the herbicide resistance development, although unfavourable to crop production, may paradoxically have very positive effects for medicinal plants such as cornflower. Plant-based food production largely relies on agrochemicals, especially pesticides, used to prevent crop losses caused by pests and pathogens. The highest share of applied pesticides is constituted by herbicides (47.5%) that are used in conventional agriculture to limit crop infestation by weeds. The use of these chemicals has resulted not only in their accumulation in water and soil but also in their presence in the food chain 2. Pesticides affect their target organisms, a range of non-target species, and plant-associated communities, including microbiota. Furthermore, biodiversity losses are observed and natural habitats for numerous non-target species decline. Eventually, herbicide exposures can lead to substantial compositional shifts within target-plant-associated communities altering interspecies interactions 3. The generalised herbicide usage has resulted in the evolution of
Journal of Economic Entomology, Aug 1, 2009
The confused ßour beetle, Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) is a st... more The confused ßour beetle, Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) is a stored-product pest that contaminates a wide range of food products, from ßour and cereals to spices. The insect reduces food quality and is responsible for large economic losses every year. Although several methods for detection of stored-product pests are common and widely used, they are time-consuming and expensive. Therefore, establishing molecular methods of detection of stored-product pests could provide a useful alternative method. We have undertaken attempts to establish methods of detection of T. confusum based on molecular biology techniques of standard and real-time polymerase chain reaction (PCR). Total DNA of T. confusum and red ßour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), used as a negative control, was isolated from insects and used as a template in standard and real-time PCR reactions. SpeciÞc primers have been designed on the basis of sequences of internal transcribed spacer (ITS) fragment of rDNA and subunit I of mitochondrial cytochrome oxidase of T. confusum available in the GenBank database. Standard PCR reactions with primers speciÞc to the ITS fragment proved to be reliable and sensitive. Real-time PCR reactions with primers speciÞc for mitochondrial DNA are considered to serve as a supplemental detection method for quantitative assessment of the infestation level.
bioRxiv (Cold Spring Harbor Laboratory), Jun 30, 2023
Root-knot nematodes (RKNs) are distributed worldwide group of plant parasitic nematodes, with a v... more Root-knot nematodes (RKNs) are distributed worldwide group of plant parasitic nematodes, with a very wide host range, including mono-and dicotyledonous hosts. Meloidogyne arenaria is, next to M. hapla, M. incognita, and M. javanica, one of the most economically important species from this genus. RKNs during parasitism hijack host metabolism to establish giant cells and to break down plant defense mechanisms. To date, studies on the interaction of RKN with maize (Zea mays L.) have been underrepresented, and a description of the early response to attack by these nematodes, vital to understanding the process, is scarce in the literature. We hypothesize that in the early stage of maize response to M. arenaria infection, significant changes in the accumulation level of proteins mainly related to plant defense response, plant cell wall modifications, and phytohormone biosynthesis can be observed. In this study, a mass spectrometry approach and a label-free quantification technique were used to assess the qualitative and quantitative composition of proteins changes in the proteome of maize roots after M. arenaria infection. We used a susceptible maize variety and carried out analyses of plant proteome at two time points: 24 hours after nematode inoculation and 3 days after inoculation. Statistical analyses of significant differences between protein intensities were performed for the datasets obtained from healthy and M. arenaria-infected plants, and differentially expressed proteins (DEPs), with both lower and higher abundance were determined. DEPs were mapped, classified to the gene ontology (GO) terms into functional categories, and assigned to appropriate Kyoto Encyclopedia of Genes and Genomes (KEGG) processes and pathways. As a result, a total of 3,743 proteins were identified with 124 DEPs at 24 hpi and 66 at 3 dpi, and significant changes in the accumulation of proteins associated with processes such as cell wall modifications, reaction to stress, as well as processes and pathways related to phenylpropanoid biosynthesis and metabolism, signal transduction and to S-adenosylmethionine biosynthesis. .
New Biotechnology, Jul 1, 2016
Research Square (Research Square), Aug 9, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Transcriptome analysis indicates the involvement of herbicide‐responsive and plant–pathogen interaction pathways in the development of resistance to <scp>ACCase</scp> inhibitors in <scp><i>Apera spica‐venti</i></scp>
Pest Management Science, Feb 21, 2023
BackgroundThe continuous use of the herbicides contributes to the emergence of the resistant popu... more BackgroundThe continuous use of the herbicides contributes to the emergence of the resistant populations of numerous weed species that are tolerant to multiple herbicides with different modes of action (multiple resistance) which is provided by non‐target‐site resistance mechanisms. In this study, we addressed the question of rapid acquisition of herbicide resistance to pinoxaden (acetyl CoA carboxylase inhibitor) in Apera spica‐venti, which endangers winter cereal crops and has high adaptation capabilities to inhabit many rural locations. To this end, de novo transcriptome of Apera spica‐venti was assembled and RNA‐sequencing analysis of plants resistant and susceptible to pinoxaden treated with this herbicide was performed.ResultsThe obtained data showed that the prime candidate genes responsible for herbicide resistance were those encoding 3‐ketoacyl‐CoA synthase 12‐like, UDP‐glycosyltransferases (UGT) including UGT75K6, UGT75E2, UGT83A1‐like, and glutathione S‐transferases (GSTs) such as GSTU1 and GSTU6. Also, such highly accelerated herbicide resistance emergence may result from the enhanced constitutive expression of a wide range of genes involved in detoxification already before herbicide treatment and may also influence response to biotic stresses, which was assumed by the detection of expression changes in genes encoding defence‐related proteins, including receptor kinase‐like Xa21. Moreover, alterations in the expression of genes associated with methylation in non‐treated herbicide‐resistant populations were identified.ConclusionThe obtained results indicated genes that may be involved in herbicide resistance. Moreover, they provide valuable insight into the possible effect of resistance on the weed interaction with the other stresses by indicating pathways associated with both abiotic and biotic stresses. © 2023 Society of Chemical Industry.
Plant and Soil
Background and aims Meloidogyne arenaria is an economically important root-knot nematode species.... more Background and aims Meloidogyne arenaria is an economically important root-knot nematode species. Successful plant infection by nematode is facilitated by parasite effectors. This study aimed to characterize a candidate M. arenaria effector, indicate its molecular partners from maize, and analyze its role during infection. Material and methods At first, we performed EST database mining to find candidate effector protein from M. arenaria. The expression of its coding gene in nematode developmental stages was assessed using digital droplet PCR. Candidate effector molecular partners were determined using yeast two-hybrid screening of maize cDNA library and interactions were confirmed by co-immunoprecipitation after co-expression in Nicotiana benthamiana. Candidate effector and its molecular partners were GFP-fused and localization in N. benthamiana leaves was observed under confocal microscope. Then, expression level of genes encoding interacting proteins from maize was measured. Resul...
Microorganisms in biological control of plant-parasitic nematodes
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Papers by Aleksandra Obrępalska-Stęplowska