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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Indonesian Journal of Chemistry Jurnal Jamu Indonesia Indonesian Journal of Applied Research (IJAR) Indonesian Journal of Jamu

Septaningsih, Dewi Anggraini

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia; Advanced Research Laboratory, Institute Of Research And Community Services, IPB University, Jl. Palem Kampus

Author-ID : 749027

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Engineering Environmental Science Health Professions Medicine & Pharmacology Other

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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Rafi, Mohamad; Wulansari, Laela; Septaningsih, Dewi Anggraini; Purnomo, Tsania Firqin; Auliatifani, Reza; Khaydanur, Khaydanur; Ilmiawati, Auliya; Yulianti, Wina; Nengsih, Nunuk Kurniati; Suparto, Irma Herawati; Kusuma, Wisnu Ananta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 Âµmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26â€“1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma mangga) Makmum Syafi’i; Eti Rohaeti; Wulan Tri Wahyuni; Mohamad Rafi; Dewi Anggraini Septaningsih
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Profil sidik jari kromatografi lapis tipis (KLT) dapat dimanfaatkan untuk kendali mutu bahan baku maupun produk tumbuhan obat. Penelitian ini bertujuan mengembangkan metode analisis sidik jari rimpang temu mangga (Curcuma mangga) untuk kendali mutunya. Rimpang kering temu mangga diekstraksi menggunakan metanol dan dianalisis sidik jari KLT. Kloroform:etil asetat (8.5:1.5) merupakan fase gerak optimum yang memisahkan 11 pita dari rimpang temu mangga dengan keterpisahan yang baik dan memiliki pita khas biru tua (Rf 0.36). Demetoksikurkumin terdeteksi pada rimpang temu mangga dengan warna jingga kecoklatan pada UV 366 nm (Rf 0.48) setelah diderivatisasi dengan pereaksi asam sulfat 10%. Pengujian spesifitas terhadap rimpang lain dalam satu genus, rimpang temu mangga menunjukan pola yang berbeda. Validasi metode analisis sidik jari KLT memenuhi kriteria keberterimaan, sehingga metode ini dapat digunakan untuk kendali mutu rimpang temu mangga.

Liquid Chromatography Mass Spectrometry (LC-MS) Fingerprint Combined with Chemometrics for Identification of Metabolites Content and Biological Activities of Curcuma aeruginosa Dewi Anggraini Septaningsih; Latifah Kosim Darusman; Farit Mochamad Afendi; Rudi Heryanto
Indonesian Journal of Chemistry Vol 18, No 1 (2018)
Publisher : Universitas Gadjah Mada

Curcuma aeruginosa is known as one of the components of herbal medicine with various biological activities. This research aims to identify the metabolites content of C. aeruginosa related to their biological activities using LC-MS fingerprint combined with chemometrics. C. aeruginosa from 3 areas in Java were collected and macerated with ethanol and then analyzed with LC-MS. Along with this analysis, the antioxidant activity of all samples was determined using CUPRAC method, and the toxicity was determined using Brine Shrimp Lethality Test (BSLT), and chemometric method was used Principle Component Analysis (PCA) and Partial Least square (PLS). Metabolites profiles showed 175 predicted compounds, in which the dominant compounds are from the sesquiterpene of Curcuma genus. The PCA metabolites profiles can separate the samples by their location of origin. Interpretation of the correlation between metabolites profiles and their bioactivities was determined using PLS technique. The results showed that the toxicity of samples was exerted by compounds with ion mass of 312.28 and 248.15, which have the highest antioxidant and toxicity potentials. Compounds with ion mass of 248.15 were predicted to be 9-Oxo-neoprocurcumenol, 7α,11α,-Epoxy-5β-hydroxy-9-guaiaen-8-one, Curcumenolactone A, or Curcumenolactone B. While compound with ion mass of 312.28 was predicted to tetrahydro-bisdemethoxycurcumin.

Phenolics Profiling and Free Radical Scavenging Activity of Annona muricata, Gynura procumbens, and Typhonium flagelliforme Leaves Extract Dewi Anggraini Septaningsih; Amalia Yunita; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.62124

The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelliforme (keladi tikus) have been used as traditional medicines in Indonesia. This study aims to determine the antioxidant capacity and putatively identified phenolics from the leaves of three medicinal plants forementioned above. We used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method for measuring radical scavenging (antioxidant assay) while the phenolics profiling was determined using UHPLC-Q-Orbitrap HRMS. The results showed that the percentage of radical scavenging activity of G. procumbens leaves extract in ethanol was higher than the other two plants. Phenolics profiling of the three medicinal plants was identified with 38 compounds belonging to flavones and flavanols hydroxycinnamic acid, and several other groups. The number of metabolites identified putatively was 12, 31, and 19 metabolites in the extracts of A. muricata, G. procumbens, and T. flagelliforme, respectively. The results confirmed the correlation between the phenolics presence and the antioxidant capacity of three plants used in this study.

Metabolite Profiling of Ebony (Diospyros celebica Bakh) Leaves and Wood Extracts Using LC-MS/MS Dien Atin Boritnaban; Alfi Hudatul Karomah; Dewi Anggraini Septaningsih; Muhammad Majiidu; Fifi Gus Dwiyanti; Iskandar Zulkarnaen Siregar; Mohamad Rafi
Indonesian Journal of Chemistry Vol 22, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.68529

Ebony (Diospyros celebica Bakh) is an endemic forest tree species found in Sulawesi whose basic information on its metabolite profile is still lacking. The objective of this research was to separate and identify putatively metabolite present in the leaves and wood of ebony across Sulawesi. Separation and identification of ebony metabolites were carried out using UHPLC-Q-Orbitrap HRMS analysis. Using ultrasonication with ethanol as the extracting solvent, we used powdered ebony leaves and wood. The results showed that the metabolites contained in the leaves and wood of ebony were 59 metabolites. About 14 compounds were found in the leaves and wood, 21 compounds in the wood, and 24 compounds in the leaves. The identified metabolites are flavonoids, terpenoids, amino aldehydes, alkaloids, quinones, steroids, amino acids, fatty acids, and saccharides. Clustering of ebony using principal component analysis obtained leaves and wood groups using peak area of known compounds as the variable.

Metabolite Profiling of Java Turmeric (Curcuma xanthoriza) Essential Oil with Different Harvest Times Mohamad Rafi; Dewi Anggraini Septaningsih; Rudi Heryanto
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Java turmeric (Curcuma xanthoriza) is one of the native plants from Indonesia. Java turmeric is known as a medicinal plant with a high content of volatile oils. In this study, we investigated the chemical profile of java turmeric essential oil obtained by hydrodistillation associated with different harvesting times of the rhizome (7, 9, and 11 months). The constituent of the essential oil was determined by using gas chromatography-mass spectrometer. About 19, 20 and 34 compounds were identified in 7, 9, and 11-month old java turmeric. Fifteen compounds were found in all samples namely β-elemene, zingiberene, γ-elemene, β-farnesene, α-curcumene, benzofuran, α-cedrene, epicurzerenone, ar-curcumene, germacrone, aromadendrene, α–longipene, trans-caryophilene, curcuphenol, and xanthorrhizol. This study showed the level of all compound detected in 7, 9, and 11 months old of java turmeric is different.

Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Mohamad Rafi; Laela Wulansari; Dewi Anggraini Septaningsih; Tsania Firqin Purnomo; Reza Auliatifani; Khaydanur Khaydanur; Auliya Ilmiawati; Wina Yulianti; Nunuk Kurniati Nengsih; Irma Herawati Suparto; Wisnu Ananta Kusuma
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.14

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 Ã‚Âµmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26Ã¢â‚¬â€œ1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

Phytochemical Profiling of Gynura procumbens (Lour.) Merr. Leaves and Stem Extracts Using UHPLC-Q-Orbitrap HRMS Dewi Anggraini Septaningsih; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.74236

In Indonesia, Gynura procumbens (Lour.) Merr., known as Longevity Spinach or Sambung Nyawa, is commonly grown in tropical and subtropical Asian countries. Many biological activities of G. procumbens have been reported. As we know, the composition and concentration of metabolites, as well as plant parts will significantly affect the biological activities. In this work, UHPLC-Q-Orbitrap-HRMS was used for the putative identification of metabolites present in 70% ethanol extract of G. procumbens leaves and stem extract. Also, we performed clustering of G. procumbens leaves and stem extracts using principal component analysis (PCA) with the peak area of the identified metabolites as the variable. Thirty-one metabolites were identified, and the number of identified peaks in the leaves is higher than in the stem. Those identified metabolites are phenolics, fatty acids, oxo monocarboxylic acids, porphyrins, and chlorophyll fragments. The PCA results showed that the leaves and stem extracts could be grouped, indicating that the composition and concentration of detected compounds differed.

UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts Alfi Hudatul Karomah; Mohamad Rafi; Dewi Anggraini Septaningsih; Auliya Ilmiawati; Utami Dyah Syafitri; Nanik Siti Aminah; Muhamad Insanu; Abdul Rohman
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.770-778

Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.

Identification of Sida rhombifolia from Its Related Plants Using Thin-Layer Chromatographic Analysis Mohamad Rafi; Sefni Reza Yolanda; Dewi Anggraini Septaningsih; Maria Bintang; Nanik Siti Aminah; Muhamad Insanu; Abdul Rohman
Indonesian Journal of Chemistry Vol 23, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.73077

Sida rhombifolia belongs to the Malvaceae family and is often used to treat gout in Indonesia. S. rhombifolia has many efficacies and contains many different chemical components. The abundance and variation of chemical content and chemical compounds in this medicinal plant are challenging factors in ensuring medicinal plants' safety and quality control. Thin-layer chromatography (TLC) fingerprint analysis derived from S. rhombifolia extract can also be used for the medicinal plant's quality control. This research aimed to develop the optimum condition for the chemical fingerprint analysis of S. rhombifolia using a TLC fingerprint analysis. A total of 11 bands were produced with optimum separation using silica gel 60 F254 TLC plate, a mixed mobile phase condition with chloroform, ethyl acetate, and methanol (6.5:2:1.5). This fingerprint analysis performed an excellent separation in the TLC plate at 366 nm with sulfuric acid as reagent derivatization. In general, the results of the analysis validation, including stability, specificity, precision, and robustness of TLC fingerprints, met the acceptance criteria. The TLC fingerprint of S. rhombifolia can be distinguished from 2 related plants with similar leaf shapes, Turnera ulmifolia L. and Hibiscus rosa-sinensis. The developed method was validated, so it could be used to control S. rhombifolia quality.

Co-Authors Abdul Rohman Abdul Rohman Alfi Hudatul Karomah Alfi Hudatul Karomah Amalia Yunita Athaya, Ardelia Auliatifani, Reza Auliya Ilmiawati Cecep Abdurohman Putra Dien Atin Boritnaban Eti Rohaeti Farit Mochamad Afendi Fifi Gus Dwiyanti Irma Herawati Suparto ISKANDAR ZULKARNAEN SIREGAR Karomah, Alfi Hudatul Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Latifah Kosim Darusman M. Rafi Madya, Muhammad Miftahul Makmum Syafi’i Maria Bintang MEGA SAFITHRI Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Muhamad Insanu Muhammad Majiidu Nanik Siti Aminah Nengsih, Nunuk Kurniati Nunuk Kurniati Nengsih Purnomo, Tsania Firqin Purwanto, Ukhradiya Magharaniq Safira Reza Auliatifani Rinaldi Idroes Rudi Heryanto Sefni Reza Yolanda Silvia, Riva Siti Aisyah SUMINAR SETIATI ACHMADI Taopik Ridwan Tsania Firqin Purnomo Utami Dyah Syafitri Wina Yulianti Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela

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