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All Journal AcTion: Aceh Nutrition Journal JSSF : Journal of Sport Science and Fitness
Gemala Anjani
Diponegoro University
Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Determination of vitamin D3 encapsulated in liprotide β-lactoglobulin crosslink oleic acid by HPLC and SEM Sylvia Rahmi Putri; Gemala Anjani; Faizah Fulyani; Diana Nur Afifah; Ahmad Syauqy; Fitriyono Ayustaningwarno
AcTion: Aceh Nutrition Journal Vol 8, No 4 (2023): December
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i4.1290

Abstract

Vitamin D is an unstable compound to light, heat, and certain chemicals, so it is easily degraded under various conditions. Therefore, instability is achieved through the encapsulation method using complex protein carrier compounds with fatty acids known as liprotide. This study aims to determine vitamin D3. Method, the determination of vitamin D3 was analyzed, including the determination of vitamin D3 standard eluents, calibration curve, and determining the retention time of vitamin D3 encapsulated in liprotide using HPLC (High-Performance Liquid Chromatography). Morphology of vitamin D3 encapsulated in liprotide using SEM (Scanning Electron Microscopy). The results showed that the standard vitamin D3 HPLC analysis using acetonitrile: methanol and acetonitrile: aquabidest obtained two peaks. Butanol: n-hexane eluent obtained one peak with a retention time of 2,170 minutes. The results of the standard calibration curve for vitamin D3 at a linearity value of R2 = 0,9997 and f(x) = 14,928x-117,930. The same retention time was obtained for vitamin D3 encapsulated in liprotide. Conclusion, an enlargement of the cubic structure, which occurred due to the encapsulation of vitamin D3 by the liprotide-forming micelles.
The Effect of Administration Ginger and Lemongrass Drink on Interleukine 6 Football Player After Exercise Miftahul adnan; Diana Nur Afifah; Gemala Anjani; Hardhono Susanto; Etika Ratna Noer; Sumardi Widodo
Journal of Sport Science and Fitness Vol. 10 No. 2 (2024): Journal of Sport Science and Fitness
Publisher : Universitas Negeri Semarang

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Abstract

Physical exercise can increase the production of free radicals. Free radical can trigger oxidative stress and inflammation. Ginger and lemongrass has bioactive compound that function as antioxidant and antiinflammatory. This study aim to determine the effecs of giving ginger and lemongras drink on IL6 level in football player. Quasi experimental study with pre and post control group design. The subject were 24 football player divided 2 namely control and intervention group. The intervention group given a ginger and lemongrass drink. The control group was only given brown sugar drink. The intervention are given after exercise for 28 days. Blood samples were collected and tested after exercise. Interleukine 6 were measured using the ELISA Method. Our result showed there was differences in interleukine 6 after giving ginger and lemongrass drink (p=0.004). The delta of interleukine 6 level after intervention was 3.24 pg/ml. Conclusion this research are there was a decrease in interleukine 6 level after giving ginger and lemongrass drink for 28 days to football players after exercise.
Co-Authors Ahmad Syauqy Diana Nur Afifah, Diana Nur Etika Ratna Noer Faizah Fulyani Fitriyono Ayustaningwarno Hardhono Susanto Miftahul adnan Sumardi Widodo Sylvia Rahmi Putri