Ni Luh Putu Eka Diarthini, Ni Luh Putu Eka
Bagian Parasitologi, Fakultas Kedokteran Universitas Udayana

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UJI EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH HIJAU “PIPER BETLE L” DAN MADU SEBAGAI ANTIMALARIA PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI Swastika, I Nengah Raka; Sri Laksemi, Dewa Ayu Agus; Damayanti, Putu Ayu Asri; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 12 No 8 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i08.P03

Abstract

Malaria is an infectious disease caused by the parasite Plasmodium sp. transmitted by the female Anopheles mosquito vector through its bite. Patients infected with malaria worldwide in the last two decades have increased, mainly due to the emergence of strains of Plasmodium falciparum that have become resistant to antimalarial drugs. This has resulted in the emergence of efforts to find alternative medicinal plants that can overcome this problem. One of the plants that have the potential to be developed is Piper betle L, or commonly known as green betel plant. This study aims to determine the effect of green betel leaf extract (Piper betle L) and honey at doses of 50, 100, and 200 mg/kgBW as antimalarials in Balb/c mice infected with Plasmodium berghei. This study is an in vivo experimental study using the Randomized Post-test Only Controlled Group method design using 24 samples of mice which were divided into four groups, one negative control group and three treatment groups. The average degree of parasitemia in the control group was 45.51%, the treatment group at a dose of 50 mg/kgBW was 35.71%, a dose of 100 mg/kgBW was 30.96% and a dose of 200 mg/kgBW was 31.67%. Based on the results of the analysis using One Way ANOVA, it was found that the mean difference between the control and treatment groups with p value <0.001 was found. The results showed that green betel leaf extract (Piper betle L) could inhibit the growth of Plasmodium berghei by looking at the mean difference between the control group and the treatment group and obtained that the average treatment group of 100 mg/kgBW and 200 mg/kgBW were equally effective at inhibiting parasitemia levels. Keywords : Antimalarial, Piper betle L, Degree of Parasitemia
GAMBARAN PENGETAHUAN DAN KONTAMINASI SOIL TRANSMITTED HELMINTHS (STH) SAYUR KUBIS (Brassica oleracea L.) DAN KEMANGI (Ocimum sanctum L.) PADA PEDAGANG LALAPAN DI DESA X, DENPASAR SELATAN Putra, I Putu Bagus Krisna Pramana; Laksemi, Dewa Ayu Agus Sri; Diarthini, Ni Luh Putu Eka; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 13 No 8 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i08.P11

Abstract

Soil Transmitted Helminths (STH) infection is caused by nematodes transmitted through the soil. In general, such as Ascaris lumbricoides, Trichuris trichiura, hookworm, or Strongyloides stercoralis. A habit of consuming not hygienic vegetables or lalapan like cabbage (Brassica oleracea L.) and basil (Ocimum sanctum L.) is a risk for transmission. Lalapan merchants play important role in food preparation, as of knowledge and good food safety are required. The study aimed to describe STH knowledge, personal hygiene, water sources, lalapan merchant’s safekeeping, and STH contamination in cabbage and basil. The study is descriptive cross-sectional with STH knowledge, STH determinants questionnaire, and microscopic identification with NaCl 0,9% sedimentation method for instruments. The sample was all lalapan merchants in X Village, South Denpasar. Total there are 26 respondents. 84,62% of respondents are male, 34,6% at age 17-25 years old, and 65,4% senior high school as tertiary education. Obtained, lalapan merchant’s knowledge is not good at 57,7%. All respondents with clean fingernails and 92,3% practiced hand washing. Water sources from municipal waterworks (76,9%), clean merchant’s water containers (88,5%), and closed (92,3%). The food safekeeping was clean, separated in 92,3%, and closed for 88.5% of respondents. Contamination was found in 8,6% of 58 samples. Hookworm eggs in three basil (10,3%) and one cabbage (3,4%), one larva STH in basil (3,4%). Although knowledge is not good, personal hygiene is widely practiced. Water sources are mostly from municipal waterworks. The food safekeeping is separated, closed, and clean. It’s necessary to improve the knowledge about STH infection to realize food safety principles. Keywords : soil transmitted helminths, STH knowledge, STH contamination
Prevalensi Infeksi Parasit Usus pada Siswa SD Negeri 5 Subagan Daerah Wisata Jasri Kabupaten Karangasem Tahun 2022 Suputra, I Wayan Bagus Abiyoga; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 12 No 12 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i12.P15

Abstract

Infeksi parasit usus merupakan salah satu penyakit tropis terabaikan yang paling banyak terjadi pada anak-anak di seluruh dunia, termasuk di Indonesia. Kejadian infeksi parasit usus sangat dipengaruhi oleh sanitasi lingkungan dan masyarakat. Penelitian ini bertujuan untuk mengetahui prevalensi infeksi parasit usus pada anak-anak SD Negeri 5 Subagan, Desa Jasri, Kabupaten Karangasem pada tahun 2022. Penelitian ini berhasil mengumpulkan spesimen tinja dan kuesioner dari 121 anak. Spesimen tinja tersebut diperiksa secara mikroskopis dengan menggunakan metode direct wet mount. Hasil penelitian menunjukkan bahwa sebanyak 10 anak (8,3%) terdeteksi mengalami infeksi parasit usus. Kasus infeksi parasit usus pada penelitian ini terdiri dari kasus infeksi tunggal yang disebabkan oleh Giardia lamblia (4/121; 3,3%), Blastocystis spp. (3/121; 2,5%), Trichuris trichiura (1/121; 0,8%), Hymenolepis diminuta (1/121; 0,8%) serta kasus infeksi campuran yang disebabkan oleh Giardia lamblia dengan Taenia sp. (1/121; 0,8%). Penelitian ini menyimpulkan bahwa prevalensi infeksi parasit usus pada anak-anak SD Negeri 5 Subagan, Desa Jasri, Kabupaten Karangasem tahun 2022 termasuk dalam kategori rendah.
PERBANDINGAN PENDEKATAN BELAJAR (LEARNING APPROACHES) MAHASISWA TAHUN PERTAMA DAN TAHUN KETIGA FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA Sanjiwani, Made Indira Dianti; Wardani, Ni Putu; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 11 No 1 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.v11.i01.P01

Abstract

Learning approach is a method applied by individuals in studying material to achieve learning outcomes. Learning approaches can be classified into surface, deep, and strategic learning approach. Changes in the learning environment may affect the learning process of a student. This is prone to occur in first-year medical students who are experiencing a transition from high school education to university. The purpose of this study was to explore the comparison of the learning approaches used by first and third-year medical students. This study is an analytical study with a cross-sectional approach using the Indonesian version of The Approaches and Study Skills Inventory for Students (ASSIST) questionnaire. The sample collection technique used random sampling method with a sample size of 216 students. The collected data were analyzed in univariate and bivariate ways using a computer program. Respondents ranging from 18 to 22 years old and most of them were females (66.2%). First-year student respondents were 106 people (49.1%) and third-year students were 110 people (50.9%). Comparative analysis for categorical data was carried out using the chi-square test and obtained p-value = 0.011 (p <0.05), indicates that there is a significant difference between the types of learning approaches of first and third-year medical students. Third-year students were found to have a deep learning approach dominantly, while first-year students have a surface learning approach dominantly. Further research related to learning approaches should be carried out as a contribution to the curriculum formation process and may encourage students to adopt a good learning approach.
Tingkat Pengetahuan dan Sikap Santri Terhadap Skabies di Pondok Pesantren Syafa'ah Darussalam Bali kumara, i made bagus bayu; Swastika, I Kadek; Sudarmaja, I Made; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 12 No 9 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i09.P10

Abstract

Latar belakang: Skabies atau yang lebih dikenal kudis adalah infestasi dari Sarcoptes scabiei varian hominis yang menyebabkan penyakit kulit. Pada tahun 2008 berdasarkan data Depkes RI, tercatat angka kejadian skabies yaitu sebesar 5,6% sampai 12,95% dengan insiden tertinggi terjadi di Pondok pesantren.Rancangan penelitian ini yaitu deskriptif kuantitatif dengan menggunakan metode Cross Sectional Study. Penelitian ini menggunakan metode total sampling kepada semua santri yang ada di Pesantren. Responden yang menjadi sampel dalam penelitian ini adalah santri di Pesantren Syafa’ah Darussalam Bali dimana jumlah responden yaitu 63 orang. kelompok usia santri yang paling banyak yaitu usai 13 tahun berjumlah 27 orang (42,8%), dan usia yang paling sedikit yaitu usia 15 tahun berjumlah 15 orang (23,8%). Tingkat pengetahuan santri terhadap skabies dengan kategori baik berjumlah 14 orang (22,2%), 35 orang (55,6%) dengan kategori cukup, 14 orang (22,2%) dengan kategori kurang. Sikap santri dalam upaya pencegahan skabies dengan kategori baik berjumlah 45 orang (71,4%), kategori cukup berjumlah 18 orang (28,6) dan kategori kurang berjumlah 0 orang (0%). Kata kunci: skabies, tingkat pengetahuan, sikap
PREVALENSI INFEKSI CACING USUS PADA SISWA SEKOLAH DASAR DI DESA PED KABUPATEN KLUNGKUNG Dyatmika, I Nyoman Adidana Jaya; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Damayanti, Putu Ayu Asri
E-Jurnal Medika Udayana Vol 12 No 8 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i08.P05

Abstract

Background : Intestinal worms is still a health problem in Indonesia, intestinal worm infections are included in neglected disease, which means this disease has not received special attention from the sufferer. Ped Village, Klungkung Regency is an area with warm temperatures, sandy loamy soil, and clean water has not been reached. This factors raise the suspicion that in Ped Village, Klungkung Regency there is one or more species of intestinal worms, so it is very important to determine the prevalence of intestinal worm infections in elementary school students Ped Village, Klungkung Regency. Aims : To determine prevalence of intestinal worm infection in elementary school students in Ped Village, Klungkung Regency. Methods : This study uses a descriptive cross sectional method, where researchers will determine the prevalence of intestinal worm infection through fecal samples examined by the kato-katz method, intestinal worms are identified under a microscope with an objective magnification of 10x to 40x. Results : There were 139 respondents who met the inclusion criteria of the study, including 72 men and 67 women, from the results of laboratory tests, prevalence of intestinal worm infection was 0.7%. Infection occurs in male children with the worm species Enterobius vermicularis. Conclusion : The prevalence of intestinal worm infection was 0.7% in elementary school students Ped Village, the species found was Enterobius vermicularis in boys. The cause of this infection is thought due to environmental and personal hygiene factors which are still not well maintained in students. Keywords: Intestinal worm infection, prevalence, elementary school children, Ped village
THE EFFECT OF PANDAN WANGI ROOTS ETHANOL EXTRACT (Pandanus amaryllifolius Roxb.) ON THE DEGREE OF PLASMODIUM BERGHEI PARASITEMIA IN VIVO Saraswati Devi, Ni Made Kumbha Mella; Laksemi, Dewa Ayu Agus Sri; Diarthini, Ni Luh Putu Eka; Sudarmaja, I Made
E-Jurnal Medika Udayana Vol 13 No 2 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i02.P13

Abstract

Malaria is an disease caused by Plasmodium genus and transmitted by female Anopheles mosquitoes via bites. The occurrence of antimalarial drug resistance in various places, such as Chloroquine and Artemisinin Combination Therapy, be a challenge for researchers to find new herbal and effective alternative antimalarial drugs. Pandan Wangi roots (Pandanus amaryllifolius Roxb.) contain compounds with antimalarial properties. This study aims to determine the effect of Pandan Wangi roots ethanol extract at doses of 1, 100, and 1,000 mg/kgBW (Body Weight on the degree of Plasmodium berghei parasitemia in vivo. This study employs an experimental in vivo method using the Post-Test Only Controlled Group Design. Sample of 24 mice were divided into one negative control group and three treatment groups. The data analysis technique employed is One Way ANOVA and Post Hoc Test. In this study, the mean degree of parasitemia in negative control group was 32.60%, treatment group with dose of 1 mg/kgBW was 22.27%, treatment group with dose of 100 mg/kgBW was 18.49%, and treatment group with dose of 1,000 mg/kgBW was 14.88%. The One Way ANOVA analysis resulted in p-value of 0.001, which is < 0.05, indicating a significant difference in the average degree of parasitemia among the three treatment groups. Thus, the effect of Pandan Wangi roots ethanol extract has antimalarial activity. Administration dose of 1,000 mg/kgBW proved to be the most effective in suppressing the growth of Plasmodium berghei parasitemia. Keywords : Pandanus amaryllifolius Roxb., Antimalarial, Plasmodium berghei, Degree of Parasitemia
GAMBARAN TINGKAT KECEMASAN MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA SEMESTER PERTAMA DALAM MENGHADAPI UJIAN OBJECTIVE STRUCTURED CLINICAL EXAMINATION Harisutha, Made Dwiki Pradnyana; Diarthini, Ni Luh Putu Eka; Laksemi, Dewa Ayu Agus Sri; Wardani, Ni Putu
E-Jurnal Medika Udayana Vol 11 No 3 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i03.P05

Abstract

Anxiety experienced by medical students when compared to the general population of the same age has a greater prevalence and is at a higher level. Anxiety can be one of some factors that can affect exam performances which can be a determining factor in medical students’ academic achievement. One of the conditions that can cause medical students to experience anxiety in the educational process is the result of stress in facing the Objective Structured Clinical Examination (OSCE). The OSCE results can be affected by students’ exam performances. This study aims to describe the anxiety levels in first semester students of the Udayana University Faculty of Medicine in facing OSCE. This study applied cross-sectional descriptive method using primary data of anxiety levels on first semester medical students who will face OSCE for the first time dan secondary data of students’ OSCE results. Zung Self-Rating Anxiety Scale (ZSAS) questionnaire were used to collect the primary data which were retrieved only once for each student. During the study, 106 respondents data were collected, consisting of 17 to 21 years range of age, and most of them were females (61.32%). This study was resulting in the form of a description of the level of anxiety towards OSCE with the most occur anxiety level towards OSCE in medical students is mild anxiety level (84.91%), followed by moderate anxiety level (14,15%), and severe anxiety level (0,94%). The results can be used to present a basic data to conduct further research in Udayana University Faculty of Medicine as a contribution for improving student readiness in facing OSCE. Keywords: anxiety levels, objective structured clinical examination, medical students.
THE EFFECT OF JOHAR PLANT FLOWER EXTRACT “CASSIA SIAMEA” ON THE DEGREE OF PLASMODIUM BERGHEI PARASITEMIA IN VIVO Pandango, Ivana Griselarosa Koni; Laksemi, Dewa Ayu Agus Sri; Sudarmaja, I Made; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 13 No 3 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i03.P09

Abstract

Malaria is a disease caused by a parasite of the genus Plasmodium sp. that spreads through the bite of the female Anopheles mosquito. Malaria is still a global problem because almost half of the world’s population is at risk of malaria which is endemic to 86 tropical and subtropical countries. In addition, resistance to antimalarial drugs that occur in some places makes many researchers try to find antimalarial drugs made from natural ingredients so that they can reduce the risk of resistance with minimal effects. One natural ingredient that is thought to be used as an antimalarial drug is the johar flower (Cassia siamea). The objective of this study is to determine the effect of johar flower extract (Cassia siamea) at doses of 10 mg/kgBW, 100 mg/kgBW, and 1000 mg/kgBW on the degree of parasitemia in male mice Balb/c strain infected with Plasmodium berghei. This study is an experimental study in vivo using the design of the Randomized Post-test Only Controlled Group method using 24 samples of mice which were divided into four groups, one negative control group and three treatment groups. The average degree of parasitemia was obtained in the control group of 24.9%, the 10 mg/kgBW dose treatment group of 13.4%, the 100 mg/kgBW dose treatment group of 10.3% and the 1000 mg/kgBW dose treatment group of 12.2%. Based on the results of the analysis with One-way ANOVA, an average difference was found between the control and treatment groups with a p-value <0.001. The result showed that giving johar flower extract (Cassia siamea) had an influence on antimalarial activity in reducing the percentage of parasitemia of mice infected with Plasmodium berghei by looking at the average difference between the control group and the treatment and obtained the average results of the treatment group of 100 mg/kgBW and 1000 mg/kgBW had the highest influence in reducing the degree of parasitemia. Keywords: Cassia siamea, Antimalaria, Plasmodium berghei, Degree of Parasitemia
PENGETAHUAN, SIKAP, DAN PERILAKU TERHADAP DEMAM BERDARAH DENGUE PADA WISATAWAN DI KECAMATAN UBUD, GIANYAR, BALI Putri, Luh Kadek Meilina; Damayanti, Putu Ayu Asri; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 11 No 3 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i03.P02

Abstract

ABSTRAK Demam Berdarah Dengue (DBD) menjadi salah satu masalah kesehatan pada dunia pariwisata di Indonesia. Nyamuk Aedes sebagai vektor virus dengue bisa menularkan DBD pada wisatawan yang berkunjung ke wilayah endemis, salah satunya di Provinsi Bali. Penelitian ini bertujuan untuk mengetahui perbedaan pengetahuan, sikap, serta perilaku terhadap DBD antara wisatawan asing dan lokal, serta hubungan antara tingkat pengetahuan dengan sikap, pengetahuan dengan perilaku, serta sikap dengan perilaku terhadap DBD pada wisatawan di Kecamatan Ubud, Gianyar, Bali. Jenis penelitian adalah analitik dengan pendekatan cross-sectional. Sampel penelitian diambil dengan teknik consecutive sampling dengan 138 wisatawan yang berkunjung di Ubud, Gianyar, Bali pada bulan Agustus-September 2020. Analisis perbedaan dengan uji chi square dan analisis hubungan dengan uji korelasi Spearman Rank. Hasil penelitian didapat 80,3% wisatawan lokal dan 58,2% wisatawan asing memiliki pengetahuan baik. Sikap positif pada wisatawan lokal sebanyak 93% sementara pada wisatawan asing 34,3%. Sedangkan untuk perilaku pencegahan DBD, wisatawan lokal yang memiliki perilaku baik sebanyak 84,5% sedangkan pada wisatawan asing sebesar 52,2%. Pengetahuan, sikap, dan perilaku tentang DBD antara wisatawan lokal dan asing didapatkan perbedaan yang bermakna masing-masing dengan nilai p 0,005; <0,001; dan <0,001. Terdapat hubungan yang signifikan antara pengetahuan dengan sikap (p < 0,001, r = 0,398), pengetahuan dengan perilaku (p < 0,001, r = 0,303), serta sikap dengan perilaku (p < 0,001, r = 0,482) terhadap DBD. Temuan ini bermanfaat dalam pengembangan informasi mengenai DBD pada pelayanan kesehatan serta dalam travel medicine kepada wisatawan. Kata kunci: pengetahuan, sikap, perilaku, demam berdarah dengue, wisatawan