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Angiogenesis in Ischemic Stroke Patients Aged 30 - 80 years at Gatot Subroto Army Central Hospital (RSPAD) Jakarta Hidayat, Andri; Arief, Mansyur; Wijaya, Andi; As’ad, Suryani
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.898 KB)

Abstract

Background: Angiogenesis, is one of the neurorepair process which plays an important role after ischemic stroke incident. vascular-endothelial growth factor (VEGF), Angiopoietin-1 (ANG1) and fibroblast growth factor (FGF) are the angiogenic factors involved in the process of angiogenesis. In this study we observed VEGF, ANG1 and FGF concentration to represent the angiogenesis process occurred in ischemic stroke patients with different onset time. Methods: This was a cross sectional study involving 63 ischemic stroke subjects aged 30 – 80 years old from The Central Hospital of the Army (RSPAD) Gatot Subroto Jakarta. Subjects were divided into 3 groups due to stroke onset time.: < than 7 days (Group A: 11 subjects), onset 7 – 30 days (Group B: 26 subjects) and > 30 days (Group C: 26 Subjects). VEGF, FGF and ANG1 serum levels were measured using multiplex method with luminex Magpix instrument. Results: VEGF, ANG1, and FGF were not significantly different between all groups. We did not find any significant correlation in all groups except FGF with ANG1 in group C. VEGF and ANG1 levels found to be highest in group B, and FGF levels in group A. Conclusions: VEGF, FGF and ANG1 increased soon after ischemic injury. FGF immediately increased in first week after onset and then decreased. VEGF and ANG1 levels reach their peak levels between 7-30 days after injury, showed that the optimal process of angiogenesis occurs in this period. There is no significant correlation between VEGF, FGF and ANG1 in all groups but we found a correlation between FGF and ANG1 in subjects with onset > 30 days after injury.
Dynamics of Human Mesenchymal Stem Cells, M1 Microglia/Macrophage, and Fractalkine in Ischemic Stroke Patients Herminawati, Lucia; Wijaya, Andi; Arief, Mansyur; As’ad, Suryani
BALI MEDICAL JOURNAL Vol 5 No 1 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: About 85% of strokes are ischemic strokes, caused by occlusion of cerebral artery that induced brain inflammation. A deep understanding of ischemic stroke mechanism will lead to better neurorestorative treatment. Objective: This study investigates the dynamics of human mesenchymal stem cells, fractalkine, and M1 microglia/macrophage in ischemic stroke patients. Results: We found the same fractalkine levels and M1 microglia/macrophage cells on patients with stroke onset 0 to 14 days, then decrease until 30 days of stroke onset. MSCs was increase 7 days after stroke onset, peaked by 14 days, then decreased until 30 days after stroke ischemic onset. Conclusions: This study found an interaction between microglia/macrophage, fractalkine, and MSCs on ischemic stroke patients, so therapeutic strategy could be developed.
Grade Komposisi Vitamin A Air Susu Ibu (ASI) pada Penyimpanan Microkontroler Arduino Nano dengan Penyimpanan pada Lemari Pendingin dan Suhu Ruangan: Comparison Between the Grade of Vitamin A in Breast Milk Stored in Arduino Nano Microcontroller Storage and the One Stored in Refrigerator and Room Temperature Nuraiman, Nuraiman; Mardiana Ahmad; As’ad, Suryani
Poltekita : Jurnal Ilmu Kesehatan Vol. 14 No. 2 (2020): October
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v14i2.172

Abstract

Penelitan ini bertujuan membandingkan komposisi Vitamin A pada ASI yang disimpan menggunakan Mikrocontroller Arduino Nano, dengan Lemari Pendingin dan Suhu Ruangan. Penelitian ini berjenis percobaan murni. Teknik penyampelan yang digunakan adalah penyampelan purposive dan diperoleh sebanyak 45 sampel. Kadar komposisi Vitamin A ASI diuji dengan Spektrofotometrik UV-Vis, Perlakuan dilakukan dengan memompa menggunakan pompa doubell pumping kemudian disimpan menggunakan Mikrocontroller Arduino Nano, Lemari Pendingin dan Suhu Ruangan selama 8 jam, Analisa data menggunakan uji One-Way Anova. Hasil penelitian menunjukan bahwa komposisi Vitamin A ASI yang disimpan menggunakan penyimpanan Mikrocontroller Arduino Nano pada suhu 300C lebih tinggi dibanding ASI yang disimpan pada Lemari pendingin pada suhu 40C dan pada Suhu Ruangan (p=0,064). Vitamin A lebih baik disimpan menggunakan Mikrocontroller Arduino Nano.
Developmental performance in small for gestational age children with and without catch-up growth Hesti Lestari; Suryani As’ad; Irawan Yusuf; Adrian Umboh; Andi Dwi Bahagia Febriani
Paediatrica Indonesiana Vol 55 No 4 (2015): July 2015
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.027 KB) | DOI: 10.14238/pi55.4.2015.199-202

Abstract

Background Infants born small for gestational age (SGA) have an increased risk of developmental delay. The influence of catch-up growth on developmental function remains unknown.Objective To compare the development of SGA children with and without catch-up growth.Methods We conducted a cross-sectional study in Manado from March to July 2013. Subjects were children aged 2-3 years, that born SGA from March 2010 to June 2011 in Prof. Dr. R.D. Kandou Hospital. Catch-up growth was defined as height-for-age more than -2SD on the 2006 WHO growth chart, and subjects were classified into the with and without catch-up growth groups. Developmental status was assessed using the Ages and Stages Questionnaire (ASQ) 3rd edition, through interviews with parents. We compared the developmental status between the with and without catch-up growth groups used Mann-Whitney test with a significance level of P < 0.05.Results Of the 112 SGA children, 66 (58.9%) had catch-up growth and 46 (41.1%) did not. The SGA children with catch up growth had significant better development performances of gross motor, fine motor, and problem solving. The mean ASQ centiles of the with and without catch-up groups were 55.15 (SD 7.843) [95%CI 53.52 to 57.08] and 48.80 (SD 11.264) [95%CI 45.46 to 52.15] in gross motor, respectively; 42.5 (SD 13.163) [95%CI 39.26 to 45.74] and 32.93 (SD 14.475) [95%CI 28.64 to 37.23] in fine motor, respectively; 46.74 (SD 13.112) [95%CI 43.52 to 49.97] and 40.98 (SD 11.480) [95%CI 37.57 to 44.39] in problem solving, respectively.Conclusion Small for gestational age children with catch-up growth have significantly better gross motor, fine motor, and problem-solving performance than those without catch-up growth.