Papers by Deborah A Cook
Protein Profiles of Mannitol-Shocked Soybean Cell Suspension Cultures
Biodeterioration Research, 1990
Water deficit stress causes numerous cellular and biochemical consequences in plants. When the in... more Water deficit stress causes numerous cellular and biochemical consequences in plants. When the individual consequences of water stress are expanded to a regional if not global level, they involve periods of drought and concomitantly reduced crop yields. Water deficit stress is induced by the addition of a nonpermeating osmoticum, such as either mannitol or polyethylene glycol (PEG) to the growth medium (Bressan et al., 1981; Bressan, et al. 1982; Handa et al., 1982; Hasegawa et al., 1984; Heyser and Nabors, 1981; Premecz et al., 1978).
Plant Cell, Tissue and Organ Culture, 1989
Organogenic callus cultures of seashore mallow, Kosteletzkya virginica (L.) Presl., originated fr... more Organogenic callus cultures of seashore mallow, Kosteletzkya virginica (L.) Presl., originated from excised mature embryos or stem sections of aseptically germinated plants initially cultured on Murashige & Skoog minimal organics medium containing 30000 mg 1glucose, 2.0 mg 1-~ indoleacetic acid and 1.0 mg 1-~ kinetin. Plants were regenerated via shoots and roots from callus cultures following transfer through a series of media with different cytokinin/auxin ratios and changes in carbohydrate source. Meristematic regions, shoot and root primordia were observed during histological examination of the tissues. Somatic embryos were not found.
Microporous and Mesoporous Materials, 1998
Heulandite-clinoptilolite zeolites can be synthesized over the complete range of Si/Al ratio (2.5... more Heulandite-clinoptilolite zeolites can be synthesized over the complete range of Si/Al ratio (2.5 to 6) found for the natural minerals. The unit cell parameters, framework infrared vibrational characteristics and thermal properties are similar to those of their natural counterparts. Results of environmental studies involving the removal of toxic metal ions such as lead, strontium and cesium from solution show that though natural clinoptilolite efficiently removes these ions from solution, the higher aluminum content of heulandite results in an improvement in the capacity of this topology for divalent cations. The ability to readily prepare synthetic heulandite and clinoptilolite using environmentally benign methods is expected to lead to improvements in the use of this family of zeolites for similar environmental applications.
Journal of Experimental Botany, 1993
In the process of selection for salt tolerance, it is important to understand the physiological b... more In the process of selection for salt tolerance, it is important to understand the physiological basis of ion management executed by the cells through the exclusion, accumulation or maintenance of ratios of specific ions. Intracellular accumulation of Na + , K + and Cl ions in the cells in vitro was studied as a factor in salt tolerance in suspension cultures of Medicago media cv. Rambler. Cells selected for NaCl tolerance (R151-S) and the non-selected R151 (normal) cells were grown in MS medium containing 0, 100, 250, 400 and 550 mM NaCl, Na + , Clor K + for 5 weeks and examined for relative growth rate per week (RGR/week) and intracellular concentration of Na + , Cl and K + ions. R151-S cells were found to be significantly more tolerant to the elevated Na + and Cl levels than R151. When the effects of Na + and Cl ions were compared, R151-S appeared to be more tolerant of higher concentrations of Clas compared to the equimolar concentrations of Na +. As the concentration of NaCl in the medium increased, the intracellular concentrations of Na + increased, though not at a proportional rate. Also, when the external Cl concentration increased, there was no significant change in the intracellular accumulation of Na +. On the contrary, intracellular Na + decreased when the external K + was increased. In the cells adapted to high concentration of NaCl, the concentration of intracellular K + was lower than the non-adapted cells. The adequate intracellular K + concentration for optimum growth in M. media suspension cultures was found to be 3000 µM/g dry weight. Excess or deficiency of intracellular K + caused severe reduction in the growth rates in all cell lines.
This is a synopsis of a dissertation on the topic.
Conference Presentations by Deborah A Cook
Southern Regional Grant ConferenceA, 2022
Comparing and contrasting the scientific method with logic models required in some grant proposal... more Comparing and contrasting the scientific method with logic models required in some grant proposal applications.
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Papers by Deborah A Cook
Conference Presentations by Deborah A Cook