Vinay Dwivedi

Aktu, Biotechnology, Faculty Member

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Papers by Vinay Dwivedi

ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

Journal of Pure and Applied Microbiology

Chromium (VI) is a well-known pollutant that is present in industrially polluted soil and water, ... more Chromium (VI) is a well-known pollutant that is present in industrially polluted soil and water, and has been reported to be mutagenic and carcinogenic. In the present study, we investigated the effective use of Leptolyngbya boryana (cyanobacterium) as an eco-friendly option to overcome Cr (VI) toxicity in tannery effluents. The main objective of this study was to identify the Cr reductase (ChrR) gene and its variability in the context of Cr (VI) stress. Industrial polluted soil samples were collected and processed according to standard protocols for ChrR variation and 16S rDNA gene analysis. Genomic DNA was isolated from the collected samples and the ChrR and 16S rDNA genes were amplified by PCR. Amplified 16S rDNA was sequenced and aligned with known sequences. In the present study, a strong correlation was established between the nucleotide sequences of the ChrR and 16S rDNA genes. The Minimum Inhibitory Concentration (MIC) was determined for Cr (VI), and pure strains of L. borya...

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Opium effluent treatment via biological means: A short discussion on evidences

Materials Today: Proceedings

An eco-friendly approach on different industrial wastes for production of alternate walling materials

Materials Today: Proceedings

Fatty acid synthase (FAS) machinery in the apicoplast: An efficient drug target for Plasmodium falciparum

Materials Today: Proceedings

Seaweeds as potential source of bioactive compounds with special emphasis on bioprospecting in COVID-19 situation

Bioprospecting of Microbial Diversity, 2022

Media optimization for cultivation freshwater microalga Scenedesmussp. under controlled conditions

NATIONAL JOURNAL OF LIFE SCIENCES, 2020

Microalgae are photoautotrophic organisms found in diverse habitat of fresh water as well as sea ... more Microalgae are photoautotrophic organisms found in diverse habitat of fresh water as well as sea water and have several applications in aquaculture, nutraceuticals and CO mitigation etc. Microalgae can also be utilized in 2 production of biofuels, lipids, proteins and many high value chemicals. The large-scale production of biofuels and other value-added products from algae has several challenges and the most effective growing media with a robust contamination control strategy are critical for economic production of algal biomass. In this study, media recipes were optimized for cultivation of Scenedesmus sp. under laboratory conditions. Three media were used with and without 0.25% sodium bicarbonate. The results revealed that the Bold's Basel Media (BBM) with and without sodium bicarbonate were most effective for the growth of Scenedesmus sp. and the highest growth (4.51 OD) was observed in BBM with sodium bicarbonate followed by (4 OD) BBM media without addition of sodium bicarbonate. Both media recipes yielded statistically higher biomass growth compared to other media. The nutrient consumption and Fv/Fm data of Scenedesmus sp. in BBM with sodium bicarbonate was comparable with the BBM without sodium bicarbonate containing media. As path forward these media compositions are required to be validated at larger scale outdoor.

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Impact of Climate Change on Localized Plant–Microbe Signalling and Technology Advancement in Microbial Quorum Sensing

Soil Biology, 2021

Identification, Cultivation and Potential Utilization of Microalgae in Domestic Wastewater Treatment

Phycology-Based Approaches for Wastewater Treatment and Resource Recovery, 2021

Characterization and Growth Evaluation of Marine Chlorella sp. for Biomass Production

Acta Scientific Microbiology, 2021

The fresh water and nutrients are costly inputs in algal cultivation. Nowadays, marine algae are ... more The fresh water and nutrients are costly inputs in algal cultivation. Nowadays, marine algae are in focus since they can be cultivated in seawater with minimum freshwater addition. In this study, marine Chlorella sp. was characterized in lab and open ponds (1m 2) of greenhouse to evaluate the potential of biomass production. In lab studies, the growth performances (OD and biomass production) of algae in industrial grade nutrient sources were found similar to lab grade sources. The growth at 12:12 h. of light: dark cycle was at par with 24 h. of continuous light illumination. The minimum inhibitory concentrations for hydrogen peroxide, benzalkonium chloride, and sodium hypochlorite were determined to be 2.5, 5, and 5 mgL-1 respectively. In pond studies, the strain was found to tolerate salinity up to 5.5%. Highest aerial, volumetric productivities and nutrient removal were observed at 10 cm as compared to 15 and 20 cm depths. The aerial productivities and biomass composition in semiturbidostat mode cultivation at 0.5 and 0.7 OD's of harvest were found comparable and semiturbidostat mode was found more productive than batch mode. Overall study showed that the marine Chlorella sp. is a robust strain and can be cultivated in open ponds using seawater.

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Experimental Review on “Evaluation of Antimicrobial activity of Metabolites from the fruit coat of Cucumis Sativus”

International Journal of Advanced Engineering Research and Science, 2020

Synergistic effect of graphene oxide coated nanotised apigenin with paclitaxel (GO-NA/PTX): A ROS dependent mitochondrial mediated apoptosis in ovarian cancer

Anti-cancer agents in medicinal chemistry, Jan 24, 2017

Ovarian cancer is most lethal among all gynecologic malignancies. Paclitaxel (PTX) is well used c... more Ovarian cancer is most lethal among all gynecologic malignancies. Paclitaxel (PTX) is well used chemotherapeutic regimen for cancer control; however its undesired toxicity has been always a matter of concern for clinicians. Here we used the graphene oxide coated nanotised apigenin (GO-NA) as chemo sensitizing agent to enhance the efficacy of paclitaxel to overcome the limitations of chemotherapy. GO and GO-Apigenin was prepared by modified Hummers method and the nanoparticles were characterized by dynamic light scattering and transmission electron microscopy Human ovarian adenocarcinomas (SKOV-3) cells was treated by DMSO, Group I (Control)-McCoy's 5A Medium, Group II-Paclitaxel (5nM) alone, Group III- Nanotised Apigenin (GO-NA-10μM) Group IV- Paclitaxel (5nM) + GO-NA (10μM). Cell viability and IC-50 value were determined by MTT assay, synergism by Compusyn software, ROS by DCFH-DA assay, SOD activity by commercially available kit and MMP were examined by JC-1 and mitotracker/D...

Polymorphic stages of the fresh water blue-green alga, Gomphosphaeria aponina

ALGAE, 2010

The natural growth of a population of Gomphosphaeria aponina Kützing (Chroococcales, Cyanoprocary... more The natural growth of a population of Gomphosphaeria aponina Kützing (Chroococcales, Cyanoprocaryota) was studied in a cemented freshwater tank in Allahabad, India. This population appeared to be a polymorphic species. Different species of the genus Gomphosphaeria have been segregated based on morphological features of colonies, cells and mucilage. However, these features are not well defined for different species. Our observations revealed many feature variations and, interestingly, certain features that have been described for different Gomphosphaeria species were seen in a single population. In this study, records of such variable morphological features were possible due to the availability of numerous specimens and continuous observations for more than two years. Further, this study revealed two points: (i) more detailed morphological studies are required both from nature as well as in culture to identify critical differences among the species, and (ii) molecular characterization of taxa appears to be necessary for final species settlement.

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Morphological Distinction of Cyanodermatium (Hydrococcaceae) and Radaisia (Hyellaceae) of Chroococcales (Cyanoprokaryota)

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2014

Radaisia gomontiana Sauvageau has been studied for their morphology from the material collected f... more Radaisia gomontiana Sauvageau has been studied for their morphology from the material collected from natural habitat and from cultures. The continuous collections of the organism from nature did not show any sign of baeocyte formation. However, in the seventeenth collection, after 2 months growth in a water body, it did reveal the formation of baeocytes only for 2 days, whereas under culture conditions, stages of baeocyte and monocyte formation were recorded as distinct features. The organism is identified as Radaisia (Hyellaceae) when baeocytes are observed. Its vegetative stage which is recognized as Cyanodermatium (Hydrococcaceae).

Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) & Trigonella foenum graecum (Methi) against some selected bacterial & fungal strains

Plants are very useful, self-generating machines, producing a variety of useful bioactive product... more Plants are very useful, self-generating machines, producing a variety of useful bioactive products. Keeping in view this idea, the crude aqueous extract of two medicinal plants were subjected to in vitro antimicrobial assay against human pathogenic Escherichia coli ,Pseudomonas putida (bacteria) and Malassezia furfur (fungus) by employing disc diffusion method. The zones of inhibition, minimum inhibitory concentration (MIC) were also determined. Among the plants tested Oscimu m sanctu m (Tulsi) was found to be the most effec tive against fungal strain in comparison to bacterial strain. Trigonella foenum graecum (Methi) extract showed maximum effect on E. coli. Trigonella and Oscimum both were found ineffective against Pseudomonas putida . Largest zone of inhibition (12 mm) is obtained with M. fu rfur at 100% conc. when treated with tulsi. In case of methi, largest zone of inhibition (14mm) is obtained with E. coli at 50% conc. Minimum Inhibitory Concentration (MIC) value of 5 mg/ml ...

Implications of Fungal Synthesis of Nanoparticles and Its Various Applications

Functionalized Nanomaterials II

Comparative anticancer potential of clove (Syzygium aromaticum)--an Indian spice--against cancer cell lines of various anatomical origin

Asian Pacific journal of cancer prevention : APJCP, 2011

Spices, active ingredients of Indian cooking, may play important roles in prevention and treatmen... more Spices, active ingredients of Indian cooking, may play important roles in prevention and treatment of various cancers. The objective of the present study is to compare the in vitro anticancer activities of three different extracts of Clove (Syzygium aromaticum L), a commonly used spice and food flavouring agent, against different kinds of cancer cell lines of various anatomical derivations. Water, ethanol and oil extracts were screened for anti proliferative activity against HeLa (cervical cancer), MCF-7 (ER + ve) and MDA-MB-231 (ER - ve) breast cancer, DU-145 prostate cancer and TE-13 esophageal cancer cell lines, along with normal human peripheral blood lymphocytes. Inhibition of cell proliferation was assessed using MTT assay as a vital stain. In the examined five cancer cell lines, the extracts showed different patterns of cell growth inhibition activity, with the oil extract having maximal cytotoxic activity. Morphological analysis and DAPI staining showed cytotoxicity to be a ...

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Cytotoxic potential of Indian spices (extracts) against esophageal squamous carcinoma cells

Asian Pacific journal of cancer prevention : APJCP, 2011

Diet is one of the important factors in cancer etiology and prevention. The Indian diet is partic... more Diet is one of the important factors in cancer etiology and prevention. The Indian diet is particularly interesting in its many unique dietary constituents, including spices like chili pepper, cloves, black pepper and black cumin, that have promise as chemopreventive agents. The objective of the present study was to compare the in vitro anticancer activities of aqueous and ethanolic extracts against the TE-13 (esophageal squamous cell carcinoma) cell line. All extracts showed cytotoxic activity but aqueous extracts were found to be more potent than alcoholic extracts. Morphological analysis, DAPI staining and DNA fragmentation assays showed maximum cell death and apoptotic cell demise (88%) to occur within 24 hours with an aqueous extract of chili pepper at 300 μl/ml.

Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) & Trigonella foenum graecum (Methi) against some selected bacterial & fungal strains

Enzymatic degradation of pyridine raffinate using response surface and artificial neural network simulation

Genetic Variability Screening of the Leptolyngbya Boryana with Expressed ChrR Gene for the Biotransformation of Cr (VI) to Cr (III) Reduction

Chromium is well known pollutant for its mutagenicity, and carcinogenicity in humans. Excessive u... more Chromium is well known pollutant for its mutagenicity, and carcinogenicity in humans. Excessive uses of chromium in leather tanning industries, stainless-steel production, and wood preservatives have resulted as chromium contamination in soil and water. This investigation indicates the effective use of Leptolyngbya boryana as an eco-friendly option to overcome Chromium (VI) toxicity in tannery effluents. The main objective of this research was to find out ChrR gene and its variability in the context of Cr (VI) stress. This is a novel study in the relation of Leptolyngbya boryana. Industrial polluted soil samples were collected and processed according to the standard protocols for ChrR variation and 16S rRNA gene. DNA was isolated and amplified through PCR. Amplified DNA was sequenced and aligned with the known sequences. In this study a strong co-relation was established in the nucleotide sequences of ChrR and 16S rRNA genes. MIC was determined for Cr (VI) and pure strains of Leptol...

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