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Calculated times (days) to reach 10%, 50% and 90% of degradation, based on a log-logistic model with three parameters. DT values were only calculated for the pre-exposed communities. replicates inoculated with the community pre-exposed for nine months did not allow us to calculate the degradation time (DT) for 10%, 50% and 90% removal.

Table 2 Calculated times (days) to reach 10%, 50% and 90% of degradation, based on a log-logistic model with three parameters. DT values were only calculated for the pre-exposed communities. replicates inoculated with the community pre-exposed for nine months did not allow us to calculate the degradation time (DT) for 10%, 50% and 90% removal.

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Multiple reaction monitoring (MRM) parameters and precursor/product ion pair for the analyses of MET and GUA. Table 1
Multiple reaction monitoring (MRM) parameters and precursor/product ion pair for the analyses of MET and GUA. Table 1
Fig. 1. Biodegradation of metformin (C/C0O) by communities pre-exposed (left) and not pre-exposed (right) to MET in biodegradation tests (measured by LC-MS/MS). Activated sludge was not pre-exposed but is represented in both figure (dotted orange line), as well as the abiotic control (full green line), to facilitate comparison, Lines show the fitted model (three-parameter logistic) for each condition. Points and shapes represent the average elimination from each conditions and error bars correspond to the standard deviation of the three replicates used to calculated the average biodegradation in each condition.
Fig. 1. Biodegradation of metformin (C/C0O) by communities pre-exposed (left) and not pre-exposed (right) to MET in biodegradation tests (measured by LC-MS/MS). Activated sludge was not pre-exposed but is represented in both figure (dotted orange line), as well as the abiotic control (full green line), to facilitate comparison, Lines show the fitted model (three-parameter logistic) for each condition. Points and shapes represent the average elimination from each conditions and error bars correspond to the standard deviation of the three replicates used to calculated the average biodegradation in each condition.
Fig. 2. Transformation of metformin to guanylurea. Metformin degradation (%) is based on the measured concentration in sample divided by the original measure concentration (C/Co). Guanylurea percentage (%) is based on the theoretical maximal transformation of metformin to guanylurea, at the original measured con- centration of metformin.
Fig. 2. Transformation of metformin to guanylurea. Metformin degradation (%) is based on the measured concentration in sample divided by the original measure concentration (C/Co). Guanylurea percentage (%) is based on the theoretical maximal transformation of metformin to guanylurea, at the original measured con- centration of metformin.
Fig. 3. (Left) PCoA ordination of the different communities over time, using a weighted Unifrac distance method. The gradient of color shown in the legend represents the time (d) since the inoculation of the chemostats with activated sludge. (right) NMDS ordination of the Bray-Curtiss dissimilarity between communities. Confidence ellipses level at 0.95.
Fig. 3. (Left) PCoA ordination of the different communities over time, using a weighted Unifrac distance method. The gradient of color shown in the legend represents the time (d) since the inoculation of the chemostats with activated sludge. (right) NMDS ordination of the Bray-Curtiss dissimilarity between communities. Confidence ellipses level at 0.95.
Fig. 4. (A) Differential analysis representing each genus whose abundance changed significantly (p < 0.05) over time from the original inoculum (0), in chemostat MET. Log2FoldChange is the log-ratio of observed differences between the tested sample and the original inoculum. Genera with a Log2FoldChange > 0 were enriched in comparison to the original inoculum while a Log2FoldChange < 0 indicates that the abundance of the genus decreased. Colors represent the class of the presented genus. (B) Heat map representing the abundance of each genus displayed in (A). Inocula represent the time of exposure used in biodegradation testing (0, 1, 3, 9 months).
Fig. 4. (A) Differential analysis representing each genus whose abundance changed significantly (p < 0.05) over time from the original inoculum (0), in chemostat MET. Log2FoldChange is the log-ratio of observed differences between the tested sample and the original inoculum. Genera with a Log2FoldChange > 0 were enriched in comparison to the original inoculum while a Log2FoldChange < 0 indicates that the abundance of the genus decreased. Colors represent the class of the presented genus. (B) Heat map representing the abundance of each genus displayed in (A). Inocula represent the time of exposure used in biodegradation testing (0, 1, 3, 9 months).
Fig. 5. (A) Differential analysis representing each genus whose abundance changed significantly (p < 0.01) over time from the original inoculum (0), in chemostat Blank. Log2FoldChange is the log-ratio of observed differences between the tested sample and the original inoculum. Genera with a Log2FoldChange > 0 were enriched in comparison to the original inoculum while a Log2FoldChange < 0 indicates that the abundance of the genus decreased. Colors represent the class of the presented genus. (B) Heatmap representing the abundance of each genus displayed in (A) with a Log2FoldChange > 0. (C) Heatmap representing the abundance of each genus displayed in (A) with a Log2FoldChange > 0. Inocula represent the time of exposure used in biodegradation testing (0, 1 month, 3 months 9 months).
Fig. 5. (A) Differential analysis representing each genus whose abundance changed significantly (p < 0.01) over time from the original inoculum (0), in chemostat Blank. Log2FoldChange is the log-ratio of observed differences between the tested sample and the original inoculum. Genera with a Log2FoldChange > 0 were enriched in comparison to the original inoculum while a Log2FoldChange < 0 indicates that the abundance of the genus decreased. Colors represent the class of the presented genus. (B) Heatmap representing the abundance of each genus displayed in (A) with a Log2FoldChange > 0. (C) Heatmap representing the abundance of each genus displayed in (A) with a Log2FoldChange > 0. Inocula represent the time of exposure used in biodegradation testing (0, 1 month, 3 months 9 months).
ECETOCG, 2013. TR 123 - Environmental Risk Assessment of Ionisable Compounds. Ecetoc. 1.29.19. http://www.ecetoc.org/publication/tr-123-environmental-risk-assessment- of-ionisable-compounds/.
ECETOCG, 2013. TR 123 - Environmental Risk Assessment of Ionisable Compounds. Ecetoc. 1.29.19. http://www.ecetoc.org/publication/tr-123-environmental-risk-assessment- of-ionisable-compounds/.
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