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Table 2: Cannabis sativa L. pretreated by steam explosion. Biotransformation in 50 mM buffer acetate pH 5.0 T = 50 °C, for 24, 48 and 72 h incubation The results of conversion of Cannabis sativa L., pretreated with AAS using the commercial enzymatic cocktail in combination with the arabinofuranosidase, highlighted that the yield of glucose after 72 h of bioconversion remained substantially unchanged (Table 3) whilst a higher yield in xylose was obtained with the use of the auxiliary enzyme. Analyzing the sugars recovery utilizing hemp SE pretreated as substrate, it was obtained a high yield of glucose (62.0%) and xylose (95.8 %), after 72 h of biotransformation (pH 5.0, T 50 °C and 500 rpm). The addition of the a-L-arabinofuranosidase (ara) as auxiliary enzyme did not substantially influence the xylose recovery (97.1 %) (Table 2).

Table 2 Cannabis sativa L. pretreated by steam explosion. Biotransformation in 50 mM buffer acetate pH 5.0 T = 50 °C, for 24, 48 and 72 h incubation The results of conversion of Cannabis sativa L., pretreated with AAS using the commercial enzymatic cocktail in combination with the arabinofuranosidase, highlighted that the yield of glucose after 72 h of bioconversion remained substantially unchanged (Table 3) whilst a higher yield in xylose was obtained with the use of the auxiliary enzyme. Analyzing the sugars recovery utilizing hemp SE pretreated as substrate, it was obtained a high yield of glucose (62.0%) and xylose (95.8 %), after 72 h of biotransformation (pH 5.0, T 50 °C and 500 rpm). The addition of the a-L-arabinofuranosidase (ara) as auxiliary enzyme did not substantially influence the xylose recovery (97.1 %) (Table 2).

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Table 1: Macromolecular composition of untreated and pretreated Hemp. Chemical composition (% total dry weight + standard deviations) 3.2 Effect of enzymatic hydrolysis on pretreated hemp. Influence of the applied pretreatment methods on the effectiveness of enzymatic hydrolysis, with the use of the commercial enzymatic cocktail previously described, was analyzed. The conditions of temperature and agitation adopted in saccharification experiments were those at which all the tested enzymes retained almost 80% of their initial activities. The following main effects were revealed in the different examined conditions.
Table 1: Macromolecular composition of untreated and pretreated Hemp. Chemical composition (% total dry weight + standard deviations) 3.2 Effect of enzymatic hydrolysis on pretreated hemp. Influence of the applied pretreatment methods on the effectiveness of enzymatic hydrolysis, with the use of the commercial enzymatic cocktail previously described, was analyzed. The conditions of temperature and agitation adopted in saccharification experiments were those at which all the tested enzymes retained almost 80% of their initial activities. The following main effects were revealed in the different examined conditions.
Table 3: Cannabis sativa L. pretreated by AAS. Biotransformation in 50 mM buffer acetate pH 5.0 T = 50 °C, for 24, 48 and 72 h incubation
Table 3: Cannabis sativa L. pretreated by AAS. Biotransformation in 50 mM buffer acetate pH 5.0 T = 50 °C, for 24, 48 and 72 h incubation
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