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Table 1. Mean values of the chemotactic index I. for heterotrophic bacteria responding to the extracellular chemical product: of Synechococcus and Prochlorococcus. Mean + SD were calculated from triplicate experiments. Time: time elapsed since release of the chemoattractant

Table 1 Mean values of the chemotactic index I. for heterotrophic bacteria responding to the extracellular chemical product: of Synechococcus and Prochlorococcus. Mean + SD were calculated from triplicate experiments. Time: time elapsed since release of the chemoattractant

Related Figures (4)

Fig. 1. Microfluidic chemotaxis assay. Bacteria and chemoat- tractants were injected into the channel via Inlets A and B, re- spectively. C denotes the channel's outlet. The grey box rep- resents the position 3 mm downstream of the microinjector's tip where data were acquired. Inset: Magnified schematic of the channel region denoted by the grey box, showing the initial conditions within the channel, including the initial posi- tion and width of the chemoattractant band (Che), the direc- tion of flow, and the direction of diffusion of the chemo- attractant band (D) at the onset of the experiment Chemotaxis assay and image analysis. A microflu- idic chemotaxis assay, described in detail elsewhere (Seymour et al. 2008), was applied to study the chemo- tactic response of the 3 bacterial species to the extra- cellular chemical products of Synechococcus and Pro- chlorococcus. The assay employed a 45 mm long, 3mm wide and 50 pm deep microchannel (Fig. 1). Two in- line inlet ports simultaneously introduced bacteria (Inlet A) and chemoattractants (Inlet B) into the chan- nel, at 240 pm s™! using a syringe pump. Inlet B was joined to a 100 pm wide microinjector, which gener- ated a 300 pm wide band of chemoattractants in the centre of the channel. Bacteria were advected along either side of the chemoattractant band, until flow in
Fig. 1. Microfluidic chemotaxis assay. Bacteria and chemoat- tractants were injected into the channel via Inlets A and B, re- spectively. C denotes the channel's outlet. The grey box rep- resents the position 3 mm downstream of the microinjector's tip where data were acquired. Inset: Magnified schematic of the channel region denoted by the grey box, showing the initial conditions within the channel, including the initial posi- tion and width of the chemoattractant band (Che), the direc- tion of flow, and the direction of diffusion of the chemo- attractant band (D) at the onset of the experiment Chemotaxis assay and image analysis. A microflu- idic chemotaxis assay, described in detail elsewhere (Seymour et al. 2008), was applied to study the chemo- tactic response of the 3 bacterial species to the extra- cellular chemical products of Synechococcus and Pro- chlorococcus. The assay employed a 45 mm long, 3mm wide and 50 pm deep microchannel (Fig. 1). Two in- line inlet ports simultaneously introduced bacteria (Inlet A) and chemoattractants (Inlet B) into the chan- nel, at 240 pm s™! using a syringe pump. Inlet B was joined to a 100 pm wide microinjector, which gener- ated a 300 pm wide band of chemoattractants in the centre of the channel. Bacteria were advected along either side of the chemoattractant band, until flow in
Fig. 2. Silicibacter sp. TM1040. (A) Swimming trajectories of TM1040 cells across the microfluidic channel following injection o f/2 growth medium as a chemoattractant (control). Each white path is the trajectory of a single bacterium. (B) Swimming trajecto ries of TM1040 cells within a band of Synechococcus elongatus extracellular products, demonstrating strong accumulation ir the band of chemoattractant. Note that the orientation has been rotated 90° from Fig. 1
Fig. 2. Silicibacter sp. TM1040. (A) Swimming trajectories of TM1040 cells across the microfluidic channel following injection o f/2 growth medium as a chemoattractant (control). Each white path is the trajectory of a single bacterium. (B) Swimming trajecto ries of TM1040 cells within a band of Synechococcus elongatus extracellular products, demonstrating strong accumulation ir the band of chemoattractant. Note that the orientation has been rotated 90° from Fig. 1
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MicrobiologyAquatic Microbial EcologyEcologySpatial OrientationDissolved Organic Carbon
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