Academia.eduAcademia.edu

Figure 1 – uploaded by 曼妙 楊

See full PDFdownloadDownload figure
Table 1. The content of chlorophyll-related compounds and carotenoid of the mature oval-pointed cecidomyiid gall and the in- fected-leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.

Table 1 The content of chlorophyll-related compounds and carotenoid of the mature oval-pointed cecidomyiid gall and the in- fected-leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.

Related Figures (7)

Figure 1. Morphology, color (A) and anatomy (B) of the ma- ture oval-pointed cecidomyiid gall on the lower epidermis of leaf of M. thunbergii. A mature larva, undergoing pupation, re- sides in the growth chamber.
Figure 1. Morphology, color (A) and anatomy (B) of the ma- ture oval-pointed cecidomyiid gall on the lower epidermis of leaf of M. thunbergii. A mature larva, undergoing pupation, re- sides in the growth chamber.
Table 2. Porphyrins and their mole percentage of the mature cecidomyiid gall and the infected leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.
Table 2. Porphyrins and their mole percentage of the mature cecidomyiid gall and the infected leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.
Figure 2. Pigment-protein complexes of thylakoid membranes isolated from the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii. Pigment-protein complexes are fractionated by Thornber (A) and MARS (B) electrophoretic systems. An oligomeric form (O) of the CPI complex is visible migrating between CPI and CPIL. L, leaf; G, gall. The Thornber electrophoretic system shows that the infected leaf of M. thunbergii contains both the CPI and CPII pigment-protein complexes usually found in higher plants, whereas the mature insect-induced gall contains only CPII complex (Figure 2A). CPI contains only chloro- phyll a and is derived from PSI, whereas CPII contains both chlorophyll a and b and is derived from the light-harvest- ing complex of PSI (LHCID (Markwell, 1986). By using the MARS electrophoretic system, only one (AB3) of the three chlorophyll b-containing pigment-protein complexes (AB1, AB2, and AB3) present in the thylakoid membranes of nor- mal higher plant leaf is detectable in the insect-induced gall, which is also deficient in Al (Figure 2B). Al is a constitu- ent part of the core of PSI and contains almost no chloro- phyll b. AB1, AB2, and AB3, containing either chlorophyll a or chlorophyll b, are derived from LHCI (Markwell, 1986).
Figure 2. Pigment-protein complexes of thylakoid membranes isolated from the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii. Pigment-protein complexes are fractionated by Thornber (A) and MARS (B) electrophoretic systems. An oligomeric form (O) of the CPI complex is visible migrating between CPI and CPIL. L, leaf; G, gall. The Thornber electrophoretic system shows that the infected leaf of M. thunbergii contains both the CPI and CPII pigment-protein complexes usually found in higher plants, whereas the mature insect-induced gall contains only CPII complex (Figure 2A). CPI contains only chloro- phyll a and is derived from PSI, whereas CPII contains both chlorophyll a and b and is derived from the light-harvest- ing complex of PSI (LHCID (Markwell, 1986). By using the MARS electrophoretic system, only one (AB3) of the three chlorophyll b-containing pigment-protein complexes (AB1, AB2, and AB3) present in the thylakoid membranes of nor- mal higher plant leaf is detectable in the insect-induced gall, which is also deficient in Al (Figure 2B). Al is a constitu- ent part of the core of PSI and contains almost no chloro- phyll b. AB1, AB2, and AB3, containing either chlorophyll a or chlorophyll b, are derived from LHCI (Markwell, 1986).
Table 3. Secondary metabolites of the mature cecidomyiid gall and the infected leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.
Table 3. Secondary metabolites of the mature cecidomyiid gall and the infected leaf of M. thunbergii Sieb & Zucc. The results were average of three determinations.
Figure 3. Room temperature absorption spectra of pigment- protein complexes from thylakoid membranes of the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii, fractionated by Thornber electrophoretic systems. A, B, and C are CPI, CPII, and FP of leaf, respectively; D and E are CPII and FP of gall, respectively.
Figure 3. Room temperature absorption spectra of pigment- protein complexes from thylakoid membranes of the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii, fractionated by Thornber electrophoretic systems. A, B, and C are CPI, CPII, and FP of leaf, respectively; D and E are CPII and FP of gall, respectively.
Figure 4. Room temperature absorption spectra of pigment- protein complexes from thylakoid membranes of the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii, fractionated by MARS electrophoretic systems. A, B, C, D, and E are Al, AB1, AB2, AB3, and FP of leaf, respectively; F, G, and H are A2, AB3, and FP of gall, respectively. It is widely accepted that the LHCII pigment-protein complex regulates the formation of grana in the thylakoid membrane; that is, no normal grana are formed if no LHCII complex is assembled in the chloroplast (Allen, 1992; Bennett, 1991). However, the electron microscopy which followed showed that this may not be the case.
Figure 4. Room temperature absorption spectra of pigment- protein complexes from thylakoid membranes of the mature oval-pointed cecidomyiid gall and the infected leaf of M. thunbergii, fractionated by MARS electrophoretic systems. A, B, C, D, and E are Al, AB1, AB2, AB3, and FP of leaf, respectively; F, G, and H are A2, AB3, and FP of gall, respectively. It is widely accepted that the LHCII pigment-protein complex regulates the formation of grana in the thylakoid membrane; that is, no normal grana are formed if no LHCII complex is assembled in the chloroplast (Allen, 1992; Bennett, 1991). However, the electron microscopy which followed showed that this may not be the case.
Figure 5. Ultrastructural morphology of thylakoid membrane and chloroplast from the mature cecidomyiid gall and the infected leaf of M. thunbergii. Notes: A, mature leaf; B, oval-pointed gall. The magnification of both chloroplasts was 25,000X.
Figure 5. Ultrastructural morphology of thylakoid membrane and chloroplast from the mature cecidomyiid gall and the infected leaf of M. thunbergii. Notes: A, mature leaf; B, oval-pointed gall. The magnification of both chloroplasts was 25,000X.
Related topics:
BotanyPlant BiologyBiologyProtein Complex DetectionPhotosynthetic Pigment
580 California St., Suite 400
San Francisco, CA, 94104
© 2025 Academia. All rights reserved