Academia.eduAcademia.edu

Figure 4 – uploaded by NORA ALLIOUI

See full PDFdownloadDownload figure
FicurRE 4: Chromatogram of the HPLC analysis of purified extracts from Alg.24B1 (a) and Alg.24B2 (b). Peaks corresponding to iturin (It), fengycin (Fen), and surfactin (Sf) are indicated.

Figure 4 FicurRE 4: Chromatogram of the HPLC analysis of purified extracts from Alg.24B1 (a) and Alg.24B2 (b). Peaks corresponding to iturin (It), fengycin (Fen), and surfactin (Sf) are indicated.

Related Figures (6)

TABLE 1: Primers used for PCR for gene content detection. diversity in physiological properties, as well as their capacity to produce numerous antimicrobial compounds, favor their ubiquitous distribution in soil, aquatic environments, food, and gut microbiota of arthropods and mammals. TABLE 2: Identification of bacteria based on 16S rRNA sequence homology.
TABLE 1: Primers used for PCR for gene content detection. diversity in physiological properties, as well as their capacity to produce numerous antimicrobial compounds, favor their ubiquitous distribution in soil, aquatic environments, food, and gut microbiota of arthropods and mammals. TABLE 2: Identification of bacteria based on 16S rRNA sequence homology.
Ficure 1: Effect of the Bacillus sp. strains on the in vitro growth of Z. tritici. Values were reported as means + SD of three measurements. 699 COR DD Vertical bars represent standard errors of the means. ; , and “***” show statistically significant differences between the tested Bacillus species each day separately. p values less than 0.05 were considered statistically significant.
Ficure 1: Effect of the Bacillus sp. strains on the in vitro growth of Z. tritici. Values were reported as means + SD of three measurements. 699 COR DD Vertical bars represent standard errors of the means. ; , and “***” show statistically significant differences between the tested Bacillus species each day separately. p values less than 0.05 were considered statistically significant.
Figure 3: PCR results for Alg.24B1 strain (B1). (+) positive control, (-) negative control, (M) DNA marker: Lambda/Pstl. FicureE 2: Detection of enzymatic activities in strains Alg.24B1 and Alg.24B2. (a) Protease activity. (b) B-glucanase activity. (c) Chitinase activity. (d) Effect of proteinase K on the antifungal activity of both strains: S: supernatant; S+pK: supernatant treated by proteinase K.
Figure 3: PCR results for Alg.24B1 strain (B1). (+) positive control, (-) negative control, (M) DNA marker: Lambda/Pstl. FicureE 2: Detection of enzymatic activities in strains Alg.24B1 and Alg.24B2. (a) Protease activity. (b) B-glucanase activity. (c) Chitinase activity. (d) Effect of proteinase K on the antifungal activity of both strains: S: supernatant; S+pK: supernatant treated by proteinase K.
TaBLE 3: Yield evaluation of lipopeptides from Alg.24B1 and Alg.24B2. Bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or as a signaling molecule in inter-/intraspecific interactions. However, overproduction of surfactin by B. amyloliquefa- ciens MT45 was observed at a titer of 2930mgI’, which is equivalent to half of the maximum biomass. The amounts of fengycin produced by both strains are the lowest compared with those of iturin and surfactin (Table 3), which is in agree- ment with the results reported by Chowdhury et al. [67] for B. amyloliquefaciens. The amount of fengycin could be increased when special culture conditions are applied.
TaBLE 3: Yield evaluation of lipopeptides from Alg.24B1 and Alg.24B2. Bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or as a signaling molecule in inter-/intraspecific interactions. However, overproduction of surfactin by B. amyloliquefa- ciens MT45 was observed at a titer of 2930mgI’, which is equivalent to half of the maximum biomass. The amounts of fengycin produced by both strains are the lowest compared with those of iturin and surfactin (Table 3), which is in agree- ment with the results reported by Chowdhury et al. [67] for B. amyloliquefaciens. The amount of fengycin could be increased when special culture conditions are applied.
Related topics:
Medicine
580 California St., Suite 400
San Francisco, CA, 94104
© 2025 Academia. All rights reserved