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Note: This study was presented at the ‘I Reunion de Biotecnologia aplicada a plantas medicinales y aromaticas’ (First Biotechnology Meeting on Medicinal and Aromatic Plants), Cordoba, Argentina, 2006. Figure 3. A: SDS-PAGE, B: PAGE/Gelatin. Lanes 1 and 5: RE (20 ug total proteins). Lanes 2 and 6: RE + beta-mercaptoethanol (20 ug). Lanes 3 and 7: RE + PMSF (15 pg). Lanes 4 and 8: RE + PMSF + beta- mercaptoethanol (15 pg). At the left the molecular mass of markers is indicated.

Figure 3 Note: This study was presented at the ‘I Reunion de Biotecnologia aplicada a plantas medicinales y aromaticas’ (First Biotechnology Meeting on Medicinal and Aromatic Plants), Cordoba, Argentina, 2006. Figure 3. A: SDS-PAGE, B: PAGE/Gelatin. Lanes 1 and 5: RE (20 ug total proteins). Lanes 2 and 6: RE + beta-mercaptoethanol (20 ug). Lanes 3 and 7: RE + PMSF (15 pg). Lanes 4 and 8: RE + PMSF + beta- mercaptoethanol (15 pg). At the left the molecular mass of markers is indicated.

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Figure 2. Photomicrography with PL. Starch grains in latex. Scale = 10 pm. Figure 2. Photomicrography with PL. Starch brguie Be LAL So MUCUMEINE Ue apres VV EURE V/7LVES A: Transverse section of leaf with laticifer Y configuration (arrow). B: Longitudinal section of stem with laticifer (arrow). C: Transverse section of flower with laticifer Y configuration. D: Longitudinal section of stem with Hellram test. Scale = 25 um: A; 50 um: B and D; 10 pm: C.
Figure 2. Photomicrography with PL. Starch grains in latex. Scale = 10 pm. Figure 2. Photomicrography with PL. Starch brguie Be LAL So MUCUMEINE Ue apres VV EURE V/7LVES A: Transverse section of leaf with laticifer Y configuration (arrow). B: Longitudinal section of stem with laticifer (arrow). C: Transverse section of flower with laticifer Y configuration. D: Longitudinal section of stem with Hellram test. Scale = 25 um: A; 50 um: B and D; 10 pm: C.
Table 2. Glycosidase activity: In situ protease detection allowed to observe clear areas in the coloured film. In the electrophoretic RE analysis by SDS-PAGE four major bands of apparent molecular weight of 97, 66, 45 and 40 KDa were detected. Proteolytic activity was evidenced in PAGE/gelatin as clear bands so much under reductive as non-reductive conditions. Bands were also noticed, which demonstrated activity in the presence of PMSF, thus indicating hat these enzymes didn't suffer inhibition.
Table 2. Glycosidase activity: In situ protease detection allowed to observe clear areas in the coloured film. In the electrophoretic RE analysis by SDS-PAGE four major bands of apparent molecular weight of 97, 66, 45 and 40 KDa were detected. Proteolytic activity was evidenced in PAGE/gelatin as clear bands so much under reductive as non-reductive conditions. Bands were also noticed, which demonstrated activity in the presence of PMSF, thus indicating hat these enzymes didn't suffer inhibition.
CONCLUSIONS vol 11 September-December 2006, 37-39 (Giordani, 2002). http//:www.idecefyn.com.ar
CONCLUSIONS vol 11 September-December 2006, 37-39 (Giordani, 2002). http//:www.idecefyn.com.ar
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