Academia.eduAcademia.edu

Figure 1 – uploaded by Carlo Brera

See full PDFdownloadDownload figure
Figure 1. (a) Histograms representing the distribution of the number of EAs quantified per sample. (b) Percentage of samples resulted above or below the limit of quantification (LOQ) value for each EA, the percentage of positive samples being the solid grey portion of the histogram. Of the 71 samples analysed, 62 samples (87%) were found to be contaminated with at least one of the 12 analysed EAs. In Figure la the number of EAs co-occurring in the analysed samples is reported; among the positive samples, 27% contained only one EA, while the eight samples (13%), four wheat-based and four rye-based, presented more than nine different EAs. The histograms of Figure 1b indicate the percentage of samples resulted above or below the LOQ value for each EA, the percentage of positive samples being the solid grey histogram.

Figure 1 (a) Histograms representing the distribution of the number of EAs quantified per sample. (b) Percentage of samples resulted above or below the limit of quantification (LOQ) value for each EA, the percentage of positive samples being the solid grey portion of the histogram. Of the 71 samples analysed, 62 samples (87%) were found to be contaminated with at least one of the 12 analysed EAs. In Figure la the number of EAs co-occurring in the analysed samples is reported; among the positive samples, 27% contained only one EA, while the eight samples (13%), four wheat-based and four rye-based, presented more than nine different EAs. The histograms of Figure 1b indicate the percentage of samples resulted above or below the LOQ value for each EA, the percentage of positive samples being the solid grey histogram.

Related Figures (4)

1 Precursor ion for each EAs was [M+H]'; 2 quantifier/qualifier. Table 1. Optimized mass spectrometric (MS/MS) parameters for the selected ergot alkaloids (EAs).
1 Precursor ion for each EAs was [M+H]'; 2 quantifier/qualifier. Table 1. Optimized mass spectrometric (MS/MS) parameters for the selected ergot alkaloids (EAs).
Table 2. The percentage of positive samples, the mean values (calculated with a medium bound approach), the standard deviation and the maximum contamination level obtained for each of the analysed EAs are reported.
Table 2. The percentage of positive samples, the mean values (calculated with a medium bound approach), the standard deviation and the maximum contamination level obtained for each of the analysed EAs are reported.
Figure 2. Histograms representing the percentage of -ine (grey histogram) and -inine (black histogram) content in each analysed food category. The distribution of the positive samples through the products is described in the Figure 3a, the pie chart shows that the most contaminated category was wheat bread. In the wheat bread samples, all the analysed EAs (-ine and -inine forms) were present with different co-occurrences. Of the samples, 56% were positive for ergocristine and the 46% for «-ergocryptine. The higher mean value was registered for the ergotamine (12.7 ug/kg), while the maximum value for a single EA was 314.3 ug/kg again for ergotamine. Ergosine and ergocristine were present in the 58% of rye bread samples but the contamination levels were lower than those recorded for the wheat bread samples.
Figure 2. Histograms representing the percentage of -ine (grey histogram) and -inine (black histogram) content in each analysed food category. The distribution of the positive samples through the products is described in the Figure 3a, the pie chart shows that the most contaminated category was wheat bread. In the wheat bread samples, all the analysed EAs (-ine and -inine forms) were present with different co-occurrences. Of the samples, 56% were positive for ergocristine and the 46% for «-ergocryptine. The higher mean value was registered for the ergotamine (12.7 ug/kg), while the maximum value for a single EA was 314.3 ug/kg again for ergotamine. Ergosine and ergocristine were present in the 58% of rye bread samples but the contamination levels were lower than those recorded for the wheat bread samples.
Related topics:
BiologyMedicineClaviceps purpurea
580 California St., Suite 400
San Francisco, CA, 94104
© 2025 Academia. All rights reserved